IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Acetylation of Yeast AMPK Controls Intrinsic Aging Independently of Caloric Restriction. Acetylation of Yeast AMPK Controls Intrinsic Aging Independently of Caloric Restriction. Cell (0092-8674) 2011 imex curation Lu J.Y., Lin Y.Y., Sheu J.C., Wu J.T., Lee F.J., Chen Y., Lin M.I., Chiang F.T., Tai T.Y., Berger S.L., Zhao Y., Tsai K.S., Zhu H., Chuang L.M., Boeke J.D. heng.zhu@jhmi.edu, leeming@ntu.edu.tw, jboeke@jhmi.edu 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Lu J.Y., Lin Y.Y., Sheu J.C., Wu J.T., Lee F.J., Chen Y., Lin M.I., Chiang F.T., Tai T.Y., Berger S.L., Zhao Y., Tsai K.S., Zhu H., Chuang L.M., Boeke J.D. heng.zhu@jhmi.edu, leeming@ntu.edu.tw, jboeke@jhmi.edu Accepted 2011-OCT-20 by MGT 21-Dec-2011: Contacted by JYOTI. Cell (0092-8674) 2011 Only protein-protein interactions imex curation Acetylation of Yeast AMPK Controls Intrinsic Aging Independently of Caloric Restriction. Acetylation of Yeast AMPK Controls Intrinsic Aging Independently of Caloric Restriction. Cell (0092-8674) 2011 imex curation Lu J.Y., Lin Y.Y., Sheu J.C., Wu J.T., Lee F.J., Chen Y., Lin M.I., Chiang F.T., Tai T.Y., Berger S.L., Zhao Y., Tsai K.S., Zhu H., Chuang L.M., Boeke J.D. heng.zhu@jhmi.edu, leeming@ntu.edu.tw, jboeke@jhmi.edu 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro deacetylase assay deacetylase assay predetermined predetermined participant predetermined Lu J.Y., Lin Y.Y., Sheu J.C., Wu J.T., Lee F.J., Chen Y., Lin M.I., Chiang F.T., Tai T.Y., Berger S.L., Zhao Y., Tsai K.S., Zhu H., Chuang L.M., Boeke J.D. heng.zhu@jhmi.edu, leeming@ntu.edu.tw, jboeke@jhmi.edu Accepted 2011-OCT-20 by MGT Cell (0092-8674) 2011 Only protein-protein interactions imex curation Acetylation of Yeast AMPK Controls Intrinsic Aging Independently of Caloric Restriction. Acetylation of Yeast AMPK Controls Intrinsic Aging Independently of Caloric Restriction. Cell (0092-8674) 2011 imex curation Lu J.Y., Lin Y.Y., Sheu J.C., Wu J.T., Lee F.J., Chen Y., Lin M.I., Chiang F.T., Tai T.Y., Berger S.L., Zhao Y., Tsai K.S., Zhu H., Chuang L.M., Boeke J.D. heng.zhu@jhmi.edu, leeming@ntu.edu.tw, jboeke@jhmi.edu 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Lu J.Y., Lin Y.Y., Sheu J.C., Wu J.T., Lee F.J., Chen Y., Lin M.I., Chiang F.T., Tai T.Y., Berger S.L., Zhao Y., Tsai K.S., Zhu H., Chuang L.M., Boeke J.D. heng.zhu@jhmi.edu, leeming@ntu.edu.tw, jboeke@jhmi.edu Accepted 2011-OCT-20 by MGT 21-Dec-2011: Contacted by JYOTI. Cell (0092-8674) 2011 Only protein-protein interactions imex curation hda1_yeast Histone deacetylase HDA1 HDA1 N2819 YNL021W protein protein yeast Saccharomyces cerevisiae Baker's yeast MDSVMVKKEVLENPDHDLKRKLEENKEEENSLSTTSKSKRQVIVPVCMPKIHYSPLKTGLCYDVRMRYHAKIFTSYFEYIDPHPEDPRRIYRIYKILAENGLINDPTLSGVDDLGDLMLKIPVRAATSEEILEVHTKEHLEFIESTEKMSREELLKETEKGDSVYFNNDSYASARLPCGGAIEACKAVVEGRVKNSLAVVRPPGHHAEPQAAGGFCLFSNVAVAAKNILKNYPESVRRIMILDWDIHHGNGTQKSFYQDDQVLYVSLHRFEMGKYYPGTIQGQYDQTGEGKGEGFNCNITWPVGGVGDAEYMWAFEQVVMPMGREFKPDLVIISSGFDAADGDTIGQCHVTPSCYGHMTHMLKSLARGNLCVVLEGGYNLDAIARSALSVAKVLIGEPPDELPDPLSDPKPEVIEMIDKVIRLQSKYWNCFRRRHANSGCNFNEPINDSIISKNFPLQKAIRQQQQHYLSDEFNFVTLPLVSMDLPDNTVLCTPNISESNTIIIVVHDTSDIWAKRNVISGTIDLSSSVIIDNSLDFIKWGLDRKYGIIDVNIPLTLFEPDNYSGMITSQEVLIYLWDNYIKYFPSVAKIAFIGIGDSYSGIVHLLGHRDTRAVTKTVINFLGDKQLKPLVPLVDETLSEWYFKNSLIFSNNSHQCWKENESRKPRKKFGRVLRCDTDGLNNIIEERFEEATDFILDSFEEWSDEE 4E7069E66D03264D 4E7069E66D03264D sch9_yeast Serine/threonine-protein kinase SCH9 YHR205W SCH9 KOM1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MMNFFTSKSSNQDTGFSSQHQHPNGQNNGNNNSSTAGNDNGYPCKLVSSGPCASSNNGALFTNFTLQTATPTTAISQDLYAMGTTGITSENALFQMKSMNNGISSVNNNNSNTPTIITTSQEETNAGNVHGDTGGNSLQNSEDDNFSSSSTTKCLLSSTSSLSINQREAAAAAYGPDTDIPRGKLEVTIIEARDLVTRSKDSQPYVVCTFESSEFISNGPESLGAINNNNNNNNNNQHNQNQHINNNNENTNPDAASQHHNNNSGWNGSQLPSIKEHLKKKPLYTHRSSSQLDQLNSCSSVTDPSKRSSNSSSGSSNGPKNDSSHPIWHHKTTFDVLGSHSELDISVYDAAHDHMFLGQVRLYPMIHNLAHASQHQWHSLKPRVIDEVVSGDILIKWTYKQTKKRHYGPQDFEVLRLLGKGTFGQVYQVKKKDTQRIYAMKVLSKKVIVKKNEIAHTIGERNILVTTASKSSPFIVGLKFSFQTPTDLYLVTDYMSGGELFWHLQKEGRFSEDRAKFYIAELVLALEHLHDNDIVYRDLKPENILLDANGNIALCDFGLSKADLKDRTNTFCGTTEYLAPELLLDETGYTKMVDFWSLGVLIFEMCCGWSPFFAENNQKMYQKIAFGKVKFPRDVLSQEGRSFVKGLLNRNPKHRLGAIDDGRELRAHPFFADIDWEALKQKKIPPPFKPHLVSETDTSNFDPEFTTASTSYMNKHQPMMTATPLSPAMQAKFAGFTFVDESAIDEHVNNNRKFLQNSYFMEPGSFIPGNPNLPPDEDVIDDDGDEDINDGFNQEKNMNNSHSQMDFDGDQHMDDEFVSGRFEI BA84BE0F27143AB5 sip2_yeast SNF1 protein kinase subunit beta-2 SIP2 SPM2 YGL208W G1155 Protein SPM2 SNF1-interacting protein 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MGTTTSHPAQKKQTTKKCRAPIMSDVREKPSNAQGCEPQEMDAVSKKVTELSLNKCSDSQDAGQPSREGSITKKKSTLLLRDEDEPTMPKLSVMETAVDTDSGSSSTSDDEEGDIIAQTTEPKQDASPDDDRSGHSSPREEGQQQIRAKEASGGPSEIKSSLMVPVEIRWQQGGSKVYVTGSFTKWRKMIGLIPDSDNNGSFHVKLRLLPGTHRFRFIVDNELRVSDFLPTATDQMGNFVNYIEVRQPEKNPTNEKIRSKEADSMRPPTSDRSSIALQIGKDPDDFGDGYTRFHEDLSPRPPLEYTTDIPAVFTDPSVMERYYYTLDRQQSNTDTSWLTPPQLPPQLENVILNKYYATQDQFNENNSGALPIPNHVVLNHLVTSSIKHNTLCVASIVRYKQKYVTQILYTPIESS CBB4FCE0070A563F CBB4FCE0070A563F snf1_yeast Carbon catabolite-derepressing protein kinase SNF1 CAT1 CCR1 GLC2 PAS14 Sucrose nonfermentating protein 1 D8035.20 YDR477W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSNNNTNTAPANANSSHHHHHHHHHHHHHGHGGSNSTLNNPKSSLADGAHIGNYQIVKTLGEGSFGKVKLAYHTTTGQKVALKIINKKVLAKSDMQGRIEREISYLRLLRHPHIIKLYDVIKSKDEIIMVIEYAGNELFDYIVQRDKMSEQEARRFFQQIISAVEYCHRHKIVHRDLKPENLLLDEHLNVKIADFGLSNIMTDGNFLKTSCGSPNYAAPEVISGKLYAGPEVDVWSCGVILYVMLCRRLPFDDESIPVLFKNISNGVYTLPKFLSPGAAGLIKRMLIVNPLNRISIHEIMQDDWFKVDLPEYLLPPDLKPHPEEENENNDSKKDGSSPDNDEIDDNLVNILSSTMGYEKDEIYESLESSEDTPAFNEIRDAYMLIKENKSLIKDMKANKSVSDELDTFLSQSPPTFQQQSKSHQKSQVDHETAKQHARRMASAITQQRTYHQSPFMDQYKEEDSTVSILPTSLPQIHRANMLAQGSPAASKISPLVTKKSKTRWHFGIRSRSYPLDVMGEIYIALKNLGAEWAKPSEEDLWTIKLRWKYDIGNKTNTNEKIPDLMKMVIQLFQIETNNYLVDFKFDGWESSYGDDTTVSNISEDEMSTFSAYPFLHLTTKLIMELAVNSQSN F5C63565C986C4E3 F5C63565C986C4E3 rpd3_yeast Histone deacetylase RPD3 RPD3 SDI2 YNL330C N0305 MOF6 REC3 SDS6 Transcriptional regulatory protein RPD3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVYEATPFDPITVKPSDKRRVAYFYDADVGNYAYGAGHPMKPHRIRMAHSLIMNYGLYKKMEIYRAKPATKQEMCQFHTDEYIDFLSRVTPDNLEMFKRESVKFNVGDDCPVFDGLYEYCSISGGGSMEGAARLNRGKCDVAVNYAGGLHHAKKSEASGFCYLNDIVLGIIELLRYHPRVLYIDIDVHHGDGVEEAFYTTDRVMTCSFHKYGEFFPGTGELRDIGVGAGKNYAVNVPLRDGIDDATYRSVFEPVIKKIMEWYQPSAVVLQCGGDSLSGDRLGCFNLSMEGHANCVNYVKSFGIPMMVVGGGGYTMRNVARTWCFETGLLNNVVLDKDLPYNEYYEYYGPDYKLSVRPSNMFNVNTPEYLDKVMTNIFANLENTKYAPSVQLNHTPRDAEDLGDVEEDSAEAKDTKGGSQYARDLHVEHDNEFY 34FFD72A7E7425DB 34FFD72A7E7425DB h2az_yeast Histone H2A.Z HTZ1 H2AZ YOL012C HTA3 O2345 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSGKAHGGKGKSGAKDSGSLRSQSSSARAGLQFPVGRIKRYLKRHATGRTRVGSKAAIYLTAVLEYLTAEVLELAGNAAKDLKVKRITPRHLQLAIRGDDELDSLIRATIASGGVLPHINKALLLKVEKKGSKK 1D8FCEE3C8A504E7 1D8FCEE3C8A504E7 rpd3-sip2 2 6 enzyme target enzyme target substrate substrate unspecified role unspecified role region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region naclys N-acetylated L-lysine NAcLys undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 enzyme enzyme unspecified role unspecified role region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeast Saccharomyces cerevisiae Baker's yeast deacetylation deacetylation reaction deacetylation Fig. 1C Trichostatin A (TSA) hda1-htz1 2 9 enzyme target enzyme target substrate substrate unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeast Saccharomyces cerevisiae Baker's yeast 4 enzyme enzyme unspecified role unspecified role region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeast Saccharomyces cerevisiae Baker's yeast deacetylation deacetylation reaction deacetylation Supp. Fig. 2B Trichostatin A (TSA) sip2-snf1-2 3 7 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeast Saccharomyces cerevisiae Baker's yeast 6 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeast Saccharomyces cerevisiae Baker's yeast physical association physical association Fig. 4B, 4C, 4D. The interaction is decreased in old cells but increased in rpd3-/- strain. The interaction significantly increases in acetylation mimetics (sip2-3KQ and sip2-4KQ mutants). The interaction between sip2 and snf1 is decreased in ageing cells. sch9-snf1 1 5 enzyme target enzyme target substrate substrate unspecified role unspecified role region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeast Saccharomyces cerevisiae Baker's yeast 7 enzyme enzyme unspecified role unspecified role yeast Saccharomyces cerevisiae Baker's yeast phosphorylation phosphorylation reaction phosphorylation Fig. 5A, Supp. Fig. 6F