IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Assembly Mechanism of FCT Region Type 1 Pili in Serotype M6 Streptococcus pyogenes. Assembly Mechanism of FCT Region Type 1 Pili in Serotype M6 Streptococcus pyogenes. J. Biol. Chem. (0021-9258) 2011 imex curation Nakata M., Kimura K.R., Sumitomo T., Wada S., Sugauchi A., Oiki E., Higashino M., Kreikemeyer B., Podbielski A., Okahashi N., Hamada S., Isoda R., Terao Y., Kawabata S. kawabata@dent.osaka-u.ac.jp 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions strp6 Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394) anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Nakata M., Kimura K.R., Sumitomo T., Wada S., Sugauchi A., Oiki E., Higashino M., Kreikemeyer B., Podbielski A., Okahashi N., Hamada S., Isoda R., Terao Y., Kawabata S. kawabata@dent.osaka-u.ac.jp Accepted 2011-NOV-16 by MGT 21-Dec-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation Assembly Mechanism of FCT Region Type 1 Pili in Serotype M6 Streptococcus pyogenes. Assembly Mechanism of FCT Region Type 1 Pili in Serotype M6 Streptococcus pyogenes. J. Biol. Chem. (0021-9258) 2011 imex curation Nakata M., Kimura K.R., Sumitomo T., Wada S., Sugauchi A., Oiki E., Higashino M., Kreikemeyer B., Podbielski A., Okahashi N., Hamada S., Isoda R., Terao Y., Kawabata S. kawabata@dent.osaka-u.ac.jp 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions strp6 Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394) comigration in sds comigration in sds page comigration in sds western blot western blot Immuno blot Immuno blot Nakata M., Kimura K.R., Sumitomo T., Wada S., Sugauchi A., Oiki E., Higashino M., Kreikemeyer B., Podbielski A., Okahashi N., Hamada S., Isoda R., Terao Y., Kawabata S. The K175A mutation given in this paper for the T6 molecule (as found in the VAKS sequence) is K163A in UniProt entry P18481 (S.Pyogenes M6). kawabata@dent.osaka-u.ac.jp Accepted 2011-NOV-16 by MGT J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation tee6_strp6 Trypsin-resistant surface T6 protein tee6 T6 antigen M6_Spy0160 protein protein strp6 Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394) MLACLAILAVVGLGMTRVSALSKDDTAQLKITNIEGGPTVTLYKIGEGVYNTNGDSFINFKYAEGVSLTETGPTSQEITTIANGINTGKIKPFSTENVSISNGTATYNARGASVYIALLTGATDGRTYNPILLAASYNGEGNLVTKNIDSKSNYLYGQTSVAKSSLPSITKKVTGTIDDVNKKTTSLGSVLSYSLTFELPSYTKEAVNKTVYVSDNMSEGLTFNFNSLTVEWKGKMANITEDGSVMVENTKIGIAKEVNNGFNLSFIYDSLESISPNISYKAVVNNKAIVGEEGNPNKAEFFYSNNPTKGNTYDNLDKKPDKGNGITSKEDSKIVYTYQIAFRKVDSVSKTPLIGAIFGVYDTSNKLIDIVTTNKNGYAISTQVSSGKYKIKELKAPKGYSLNTETYEITANWVTATVKTSANSKSTTYTSDKNKATDNSEQVGWLKNGIFYSIDSRPTGNDVKEAYIESTKALTDGTTFSKSNEGSGTVLLETDIPNTKLGELPSTGSIGTYLFKAIGSAAMIGAIGIYIVKRRKA 70F7251EEFDBF644 q5xe69_strp6 M6_Spy0159 protein protein strp6 Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394) MYSRLKRELVIVINRKKKYKLIRLMVTVGLIFSQLVLPIRRLGLQMISTQTKVIPQEIVTQTETQGTQVVATKQKLESENSSLKVALKRESGFEHNATIDASLDTESQGDNSQRSVTQAIVTMALELRKQGLSIVDTKIVRIQSSTNQRNDITTTLTFKNGLSLEGASTEANDPNVRVGIVNPNDTVQTITPTIKQDADGKVKNLVFTGRLGKQVIIVSTTRLKEEQTISLDSYGELVIDGAVGLSQKDRPPYSKPITVNILKPKLSSIESSLDSKDFEIVKTIDNLYTWDDQFYLLDFISKQYEVLKTDYQSAKDSTPQTRDILFGEYTVEPLVMNKGHNNTINIYIRSTRPLGLKPIGAAPALIQPRSFRSLTPRSTRMKRSAPVEKFEGELEHHKRIDYLGDNQNNPDTTIDDKEDEHDTSDLYRLYLDMTGKKNPLDILVVVDKSGSMQEGIGSVQRYRYYAQRWDDYYSQWVYHGTFDYSSYQGESFNRGQIHYRYRGIVSVSDGIRRDDAVKNSLLGVNGLLQRFVNINPENKLSVIGFQGSADYHAGKWYPDQSPRGGFYQPNLNNSRDAELLKGWSTNSLLDPNTLTALHNNGTNYHAALLKAKEILNEVKDDGRRKIMIFISDGVPTFYFGEDGYRSGNGSSNDRNNVTRSQEGSKLAIDEFKARYPNLSIYSLGVSKDINSDTASSSPVVLKYLSGEEHYYGITDTAELEKTLNKIVEDSKLSQLGISDSLSQYVDYYDKQPDVLVTRKSKVNDETEILYQKDQVQEAGKDIIDKVVFTPKTTSQPKGKVTLTFKSDYKVDDEYTYTLSFNVKASDEAYEKYKDNEGRYSEMGDSDTDYGTNQTSSGKGGLPSNSDASVNYMADGREQKLPYKHPVIQVKTVPITFTKVDADNNQKKLAGVEFELRKEDKKIVWEKGTTGSNGQLNFKYLQKGKTYYLYETKAKLGYTLPENPWEVAVANNGDIKVKHPIEGELKSKDGSYMIKNYKIYQLPSSGGRGSQIFIIVGSMTATVALLFYRRQHRKKQY 507C118865F0FFEB tee6-tee6 2 3 unspecified role unspecified role neutral component neutral component lpstg region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P18481:p.Lys163Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A Wed May 02 16:20:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys163ala' Tue Jun 30 19:08:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys163Ala' P18481:p.Thr507Ser mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T S Wed May 02 15:33:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr507ser' Tue Jun 30 19:04:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr507Ser' 3 unspecified role unspecified role neutral component neutral component P18481:p.Thr507Ser mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T S Wed May 02 19:14:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr507ser' Tue Jun 30 17:24:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr507Ser' lpstg region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P18481:p.Lys163Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A Wed May 02 19:36:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys163ala' Tue Jun 30 17:44:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys163Ala' physical association physical association Fig. 2, 3, 4, 5, 6, Supp. Fig. 4 q5xe69-tee6 2 3 unspecified role unspecified role neutral component neutral component P18481:p.Thr507Ser mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T S Wed May 02 15:20:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr507ser' Tue Jun 30 17:08:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr507Ser' P18481:p.Lys163Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A Wed May 02 17:17:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys163ala' Tue Jun 30 17:52:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys163Ala' 4 unspecified role unspecified role neutral component neutral component pss region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q5XE69:p.Ser1004Thr mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S T Wed May 02 15:00:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser1004thr' Tue Jun 30 16:58:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser1004Thr' physical association physical association Fig. 2, 3, 4, 5, 6, Supp. Fig. 5 q5xe69-tee6-2 1 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association Supp. Fig. 3