MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it PTK7/Otk interacts with Wnts and inhibits canonical Wnt signalling. PTK7/Otk interacts with Wnts and inhibits canonical Wnt signalling. EMBO J. (0261-4189) 2011 imex curation Peradziryi H., Kaplan NA., Podleschny M., Liu X., Wehner P., Borchers A., Tolwinski NS. Only protein-protein interactions drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Peradziryi H., Kaplan NA., Podleschny M., Liu X., Wehner P., Borchers A., Tolwinski NS. EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation PTK7/Otk interacts with Wnts and inhibits canonical Wnt signalling. PTK7/Otk interacts with Wnts and inhibits canonical Wnt signalling. EMBO J. (0261-4189) 2011 imex curation Peradziryi H., Kaplan NA., Podleschny M., Liu X., Wehner P., Borchers A., Tolwinski NS. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Peradziryi H., Kaplan NA., Podleschny M., Liu X., Wehner P., Borchers A., Tolwinski NS. EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation PTK7/Otk interacts with Wnts and inhibits canonical Wnt signalling. PTK7/Otk interacts with Wnts and inhibits canonical Wnt signalling. EMBO J. (0261-4189) 2011 imex curation Peradziryi H., Kaplan NA., Podleschny M., Liu X., Wehner P., Borchers A., Tolwinski NS. Only protein-protein interactions xenla-embryo Xenopus laevis embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Peradziryi H., Kaplan NA., Podleschny M., Liu X., Wehner P., Borchers A., Tolwinski NS. EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation ptk7_drome Tyrosine-protein kinase-like otk otk Tyrosine-protein kinase-like 7 homolog Gp160-Dtrk Off-track CG8967 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTARMISICGLVMALMMASVLASSSRFQRVPQSQSVVENESVKFECESTDSYSELHYDWLHNGHRIAYDKRVHQIGSNLHIEAVRRTEDVGNYVCIATNLASGAREASPPAKLSVIYLESASVQLLGSNRNELLLKCHVEGASGDLEPLEIEWYRNSEKLSTWKNVQLDQHRLIIRQPGSEDDGLYRCTASNAAGRVMSKQGYVYQSSVKCLPRLPRRKNEKMMESWDKQTFLCRGKRGGAAGLEALPAAPEDLRIVQGPIGQSIIKEGEPTALTCLYELPDELKNQRIQLRWRKDGKLLRQVELGGSAPIPGHSFDSGKDALLREDARLVLHKQNGTLSFASIIASDAGQYQCQLQLEAHAPINSSPGILEVIEQLKFVPQPTSKNLELDAVVAKVHCKAQGTPTPQVQWVRDGENTTLPDHVEVDANGTLIFRNVNSEHRGNYTCLATNSQGQINATVAINVVVTPKFSVPPVGPIETSEQGTVVMHCQAIGDPKPTIQWDKDLKYLSENNTDRERFRFLENGTLEIRNVQVEDEGSYGCTIGNSAGLKREDVQLVVKTTGDGFAPEESGGDGFLVTRAVLITMTVALAYIVLVVGLMLWCRYRRQARKARLNDLSTKEAGGDQPDVAGNGKGSEQEPCLSKQHNGHSKSRSKSSGDAQKSDDTACSQQSRASKKSAHIYEQLALPRSGLSELIQIGRGEFGDVFVGKLKATLVTSPSDKDADTEKQHSNSENGSGGSGSGSTTLSTLNEKRRSKTSMDDIEEIKEEEQDQHNQSGLEQLVLVKALNKVKDEQACQEFRRQLDLLRAISHKGVVRLFGLCREKDPHYMVLEYTDWGDLKQFLLATAGKVNTATAGSSSPPPLTTSQVLAVAYQIARGMDAIYRARFTHRDLATRNCVISSEFIVKVSYPALCKDKYSREYHKHRNTLLPIRWLAPECIQEDEYTTKSDIFAYGVVVWELFNQATKLPHEELTNEQVVQRSQAGSLEWSVAEATPDSLREILLSCWVSNPKERPSFSQLGAALSKAMQSAEK 559659540BDE66F5 ptk7_human Inactive tyrosine-protein kinase 7 CCK4 Colon carcinoma kinase 4 Protein-tyrosine kinase 7 PTK7 Pseudo tyrosine kinase receptor 7 Tyrosine-protein kinase-like 7 protein protein human Homo sapiens Human MGAARGSPARPRRLPLLSVLLLPLLGGTQTAIVFIKQPSSQDALQGRRALLRCEVEAPGPVHVYWLLDGAPVQDTERRFAQGSSLSFAAVDRLQDSGTFQCVARDDVTGEEARSANASFNIKWIEAGPVVLKHPASEAEIQPQTQVTLRCHIDGHPRPTYQWFRDGTPLSDGQSNHTVSSKERNLTLRPAGPEHSGLYSCCAHSAFGQACSSQNFTLSIADESFARVVLAPQDVVVARYEEAMFHCQFSAQPPPSLQWLFEDETPITNRSRPPHLRRATVFANGSLLLTQVRPRNAGIYRCIGQGQRGPPIILEATLHLAEIEDMPLFEPRVFTAGSEERVTCLPPKGLPEPSVWWEHAGVRLPTHGRVYQKGHELVLANIAESDAGVYTCHAANLAGQRRQDVNITVATVPSWLKKPQDSQLEEGKPGYLDCLTQATPKPTVVWYRNQMLISEDSRFEVFKNGTLRINSVEVYDGTWYRCMSSTPAGSIEAQARVQVLEKLKFTPPPQPQQCMEFDKEATVPCSATGREKPTIKWERADGSSLPEWVTDNAGTLHFARVTRDDAGNYTCIASNGPQGQIRAHVQLTVAVFITFKVEPERTTVYQGHTALLQCEAQGDPKPLIQWKGKDRILDPTKLGPRMHIFQNGSLVIHDVAPEDSGRYTCIAGNSCNIKHTEAPLYVVDKPVPEESEGPGSPPPYKMIQTIGLSVGAAVAYIIAVLGLMFYCKKRCKAKRLQKQPEGEEPEMECLNGGPLQNGQPSAEIQEEVALTSLGSGPAATNKRHSTSDKMHFPRSSLQPITTLGKSEFGEVFLAKAQGLEEGVAETLVLVKSLQSKDEQQQLDFRRELEMFGKLNHANVVRLLGLCREAEPHYMVLEYVDLGDLKQFLRISKSKDEKLKSQPLSTKQKVALCTQVALGMEHLSNNRFVHKDLAARNCLVSAQRQVKVSALGLSKDVYNSEYYHFRQAWVPLRWMSPEAILEGDFSTKSDVWAFGVLMWEVFTHGEMPHGGQADDEVLADLQAGKARLPQPEGCPSKLYRLMQRCWALSPKDRPSFSEIASALGDSTVDSKP 304926A1774EB5F4 q6pa07_xenla ptk7.L MGC68806 ptk7 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MGPIVLSAFILLVVVSTRAAILFTKEPYSQDALHGRSAILRCEVEEPVEVQFEWLQNGVPVRDTERRYQEGGSLTFTAVDRKQDGGTFQCVARNPNTGEEGRSNIAYFNIKWIESGGVTLQYPGSASDIQSSSRVILRCHIDGHPRPICLWFRDGTPLADDGQAISLSNRERTLTLHSAGPDDSGEYYCCAYNAAGSVCSNRNFTLSIIDESFPQAVVIPEDQVVNKNEDAMFHCLFSATPPPSQEWFFEDQVPLSNKSRITVFQNGSLLISSVRPRSSGVYSCVGTGYRGQKAVLKASLRLADIDKMRPLPPRVLTADMVQRITCDGPQGIPPPTVWWERDGTRVPAEGRVYQDGMELVFKPITEGDSGSYICHAANKAGERRQELQITVATVPEWLERPTDSLMEEGKPGFLHCQSKASLEPNITWYRNGNPVSKDSRFEVFPNGTLKILHVEVYDGTVYRCVSSTPAGYIDAQARVHVQENLKFTPIPRPHQCMELDKEMTVHCSATGREKPNIQWIKGDGSDLPGHVESKAGLLHFQPVTRRDAGNYTCIASNSLQGEIQAAVHLTVAVLVSFKIEPENTTVYQGHTAVLHCQAEGDPTPNIQWQSRDVMVAAKHYPRIQVMPNGSLVIYRVTNEDAGKYACIAGNACDIKHRDTFLYVVDKPPGGANEPDSPYKLIQTIVLSVVAAVTYIIIVLGLMLYCKKRRKARRHGKHEGDEPEMECLNGDALLQNGQTTAEIPEEVPLTTLSNKRHSSGDKISFPRANLHPITTLGRGEFGEVFLAKAPNPDSTSGEAVVLVKALQTRDEQLQMDFRRELDMFSKLNHANVVRLVGQCREAEPHYMVLEYIDLGDLKQFLRISRSREEKPKPLSSKHKVSLCTQVALGMEHLSNSRFVHKDLAARNCLVSAQRLVKVSALGLSKDVYSSEYHPLRQTKVPLRWMSPEAVQDDDFSTKSDVWSFGVLMWEVFTLGELPYTSLPDEEVLAGLQNGSLKLPAPEGCSSRVYRLMQRCWAPSPKDRPSFSEIVNALGDLSSGSKTQG mint 15FF0F3CA0A91786 q9y1t2_drome Apc2 E-APC CG6193 APC APC2 D-APC2 Dm APC2 Dmel\CG6193 E-APC dAPC2 apc2 d-APC2 dAPC2 dApc2 dapc2 e-apc Dmel_CG6193 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTLNESDATRLELTRNFLELSRNPETCTALRSSDCIQLLVQILHANDEGLSTAKKYASQALHNIVHNNPEEKERQREVKMLRLLDQILDYCNFLHTQLQSGGEAIADDEDRHPLAAMKLLMKASFDEEHRQTMCELGALKAIPNLVHLDHAVHGPAAGREQCNALRSYGLMALTNLTFGDENVHNKSYLCGQRQFMEVVIAQLNTAPDELLQVLAGVLRNLSWRADKHMKTIFNELGTVTSLARAAMQNKNENTLKAILSALWNLSAHCSTNKAEFCAVDGALAFLVGMLSYEGPSKTLKIIENAGGILRNVSSHIAVCEPYRQILRRYNCLAILLQQLKSESLTVVSNSCGTLWNLSARCPEDQQYLIDHNAIPLLRALISSKNSMIAEGSASALKNLVNFRATQELMPNGDGGSLPLDKEAGHGGTLPRRFSSLRLSSNPTGSLKKVRPSTVSTTGFLNRKCESRESIYSGKSDSTKYSTKSEGAKNPFEIVTPTEEQPIDYSMKYMEHKPNSSKTFEIDLDQPTDFSARYKERRSAQTAQPELKSETNEIRSKELQLTKSSSATELRNSPGLVAVSAAKQKIATETETETAERPINYCEEGTPGSFSRFDSLNSLTEKPEKCMPPKTPTKTAVLPVHVDGNTPQNIDSALETPLMFSRRSSMDSLVGDDETVACEDNGSVISEYSRMQSGVISPSELPDSPTQSMPQSPRRDRKVSTQNNLDTPEQKPSTVFEDKLNRFHVEHTPAAFSCATSLSNLSMMDDSNANAIRGQRGNDINGNGDAPRSYCTEDTTAVLSKAPSNSDLSILSIPNDLNANEAQPVPAPRADVTGMDTRMPAEDAISKMRCGGNALPSYLPVSDEMSKYYVEDSPCTFSVISGLSHLTVGSAKAGPVLKLPMRTAEEAQAPKLPPRRSAVQGDAEPRLPPKKSDSLSSLSMDSDDDCNLLSQAIAAGSCRPQPSGASTSSSLANASTSTLCRENGQSKKQVEHGDKPNYSSDDSLDDDDDDARSKSLFEQCILSGMHKSNDALESEGEPPGQRQEISARDRFVSNQVRQIESMLAGRQH DIP protein Q9Y1T2 original sequence version: 113 20C8F5F6121888F8 wnt4_drome Protein Wnt-4 Wnt4 Wnt-4 dWnt-4 CG4698 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MPSPTGVFVLMILTHLSFGLGQVRNEDQLLMVGQNGDLDSSNPAIHHQQHQQHQQHQQHQQHQSNHNLNNGNMNSTILNTLMGNNAGQVVNSSPGGGGSMINQLGSSTSSVPSVIGGGVGSVGNPWHSAVGLGVPGNGMGLPSSHGLGGNMGSHPHGHALAGLAKLGIIVPGGQGLPGNLGYGGTMLNGGGVGGAAGMGLGIGSNTNNMDMQQGLYNEHFISEHTVMAVFTSQGQVGGPCRYMPATRRQNHQCRKETGLPGTLSEARRLATTHCEEQFRYDRWNCSIETRGKRNIFKKLYKETAFVHALTAAAMTHSIARACAEGRMTKCSCGPKKHNREAQDFQWGGCNDNLKHGKRVTRSFLDLRGGDGDEVSEILRHDSEVGIEAVSSQMMDKCKCHGVSGSCSMKTCWKKMADFNATATLLRQKYNEAIRKAPNQRSMRQVSSSRMKKPKQRRKKPQQSQYTTLYYLETSPSYCAVTKDRQCLHPDNCGTLCCGRGYTTQVVKQVEKCRCRFNNGRCCQLICDYCQRLENKYFCK 6682C8B3D729D067 axn_drome Axin Axn CG7926 Axis inhibition protein d-Axin protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSGHPSGIRKHDDNECSGPRPPVPGEESRVKKMTEGVADTSKNSSPSYLNWARTLNHLLEDRDGVELFKKYVEEEAPAYNDHLNFYFACEGLKQQTDPEKIKQIIGAIYRFLRKSQLSISDDLRAQIKAIKTNPEIPLSPHIFDPMQRHVEVTIRDNIYPTFLCSEMYILYIQQMSAQQERCTSSGATGSGSAGSSGSGGSSLAGACALPPTTASGKQQLPQLVPPGAFINLPVSSVSGPPAGTCSASGSVYGPSTSASSSGSISATDTLPRSSTLPTLHEDSVLSLCDDFEKVQMQEGGGSLGSGSVGAGARAPDYPIRLTRDLLIATQKRRLEIRPPGAHGYVYNPSTTNTSYVPNSRVDSERASVSSGGRTDSDTMSISSCSMDGRPYIQRRHSSTESKAIRQSAMANKETNTFQVIPRTQRLHSNEHRPLKEEELVSLLIPKLEEVKRKRDLEERARERNPGAALLTNERSSASDRAFAEAIREKFALDEDNDQDILDQHVSRVWKDQTPHRSPGTMSPCPPIPSRRRTATHDSGMVSDGAMSLSGHSMKHSKSMPDHSSCSRKLTNKWPSMNTDSGISMFSADTVTKYKDASSRSGSSTASKLEEAKRRLEDEPRRSRRYAQPPMQHLSQQPLASFSSSSSGGSISLPHQPPPLPAKPPETIVVFSFCEEPVPYRIKIPGTQPTLRQFKDYLPRRGHFRFFFKTHCEDPDSPVIQEEIVNDSDILPLFGDKAMGLVKPSD DIP protein Q9V407 original sequence version: 122 31A502528CEE84BA fzd7a_xenla Frizzled-7-A fzd7-a fz7-a protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MSSTVSLLFCCLFLQLCPSAQQYHGEKGISVPDHGFCQPISIPLCTDIAYNQTIMPNLLGHTNQEDAGLEVHQFYPLVKVQCSPELRFFLCSMYAPVCTVLEQAIPPCRSLCERARQGCEALMNKFGFQWPERLRCENFPVHGAGEICVGQNTSDNSPSGPTARPSPYLPDSITFQPHPHRDFTCPRQLKVPPYLAYRFLGEKDCGAPCEPGKANGLMYFKEEEVRFARLWVGIWAILCCISTLFTVLTYLVDMRRFSYPERPIIFLSGCYFMVAVAYTAGFLLEERAVCVERFSEDSYRTVAQGTKKEGCTILFMILYFFGMASSIWWVILSLTWFLSAGMKWGHEAIEANSQYFHLAAWAVPAVKTITILAMGQVDGDVLSGVCYVGINSVDSLRGFVLAPLFVYLFIGTSFLLAGFVSLFRIRTIMKHDGTKTEKLEKLMVRIGVFSVMYTVPATIVLACYFYEQAFRDTWEKTWLVQTCKGYAVPCPNYNFAPMSPDFTVFMIKYLMTMIVGITSSFWIWSGKTLQSWRRFYHRLSNGSKGETAV DA44954BFF8F594B dsh_drome Segment polarity protein dishevelled dsh CG18361 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDADRGGGQETKVIYHIDDETTPYLVKIPIPSAQVTLRDFKLVLNKQNNNYKYFFKSMDADFGVVKEEIADDSTILPCFNGRVVSWLVSADGTNQSDNCSELPTSECELGMGLTNRKLQQQQQQHQQQQQQQQQQHQQQQQQQQQQVQPVQLAQQQQQQVLHHQKMMGNPLLQPPPLTYQSASVLSSDLDSTSLFGTESELTLDRDMTDYSSVQRLQVRKKPQRRKKRAPSMSRTSSYSSITDSTMSLNIITVSINMEAVNFLGISIVGQSNRGGDGGIYVGSIMKGGAVALDGRIEPGDMILQVNDVNFENMTNDEAVRVLREVVQKPGPIKLVVAKCWDPNPKGYFTIPRTEPVRPIDPGAWVAHTQALTSHDSIIADIAEPIKERLDQNNLEEIVKAMTKPDSGLEIRDRMWLKITIPNAFIGADAVNWVLENVEDVQDRREARRIVSAMLRSNYIKHTVNKLTFSEQCYYVVNEERNPNLLGRGHLHPHQLPHGHGGHALSHADTESITSDIGPLPNPPIYMPYSATYNPSHGYQPIQYGIAERHISSGSSSSDVLTSKDISASQSDITSVIHQANQLTIAAHGSNKSSGSSNRGGGGGGGGGGNNTNDQDVSVFNYVL 0BA24ED350666CF5 wnt8_xenla Protein Wnt-8 wnt8 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MQNTTLFILATLLIFCPFFTASAWSVNNFLMTGPKAYLTYSASVAVGAQNGIEECKYQFAWERWNCPESTLQLATHNGLRSATRETSFVHAISSAGVMYTLTRNCSMGDFDNCGCDDSRNGRIGGRGWVWGGCSDNAEFGERISKLFVDGLETGQDARALMNLHNNEAGRLAVKETMKRTCKCHGISGSCSIQTCWLQLAEFRDIGNHLKIKHDQALKLEMDKRKMRSGNSADNRGAIADAFSSVAGSELIFLEDSPDYCLKNISLGLQGTEGRECLQSGKNLSQWERRSCKRLCTDCGLRVEEKKTEIISSCNCKFHWCCTVKCEQCKQVVIKHFCARRERDSNMLNTKRKNRGHRR 8BC8B9E20016E504 wnt3a_xenla Protein Wnt-3a wnt3a protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MGCFGYLLLIIGLHQVLATYPIWWSLAVGQQYSSLGTQPIPCGTIPGLVAKQMRFCRNYMEIMPSVAEGVKIGIQECQHQFRGRRWNCTTVNDNLAIFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGSATICGCDTHHKGPPGEGWKWGGCSEDMDFGSMVSREFADARENRPDARSAMNRHNNEAGRTSILDHRHLKCKCHGLSGSCEVKTCWWSQPDFRVIGDYLKDKYDSASEMVVEKHRESRGWVETLRPKYTFFKPPIERDLIYYESSPNFCEPNPETGSFGTRDRECNVTSHGIDGCDLLCCGRGQNTRTEKRKEKCHCIFHWCCYVSCQECMRVYDVHTCK mint 4B5F93F5DF08D6C5 apc2-axn-1 1 Axin 9 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Apc2 7 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association homomint mint sf1d wnt4-otk 1 Wnt4 8 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Otk 4 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association mint f3a sf1c otk-dsh 1 Otk 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell binding site binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-7036265]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 1034-1034, sequence length 1033 [EBI-7036265]607-1034 ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Dsh 11 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association f3b mint ptk7-wnt3a 3 PTK7 6 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Wnt3a 13 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f4a mint ptk7-wnt8 3 PTK7 6 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Wnt8 12 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f4b mint wnt8-ptk7 3 PTK7 6 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Wnt8 12 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association sf2b mint As the extracellular domain of PTK7 (exPTK7) was precipitated by Wnt3a and Wnt8 (immunoprecipitation; Supplementary Figure S2), it is sufficient for interaction with Wnt and, therefore, likely required for the PTK7-mediated inhibition of Wnt signalling. wnt3a-ptk7 3 PTK7 6 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Wnt3a 13 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association sf2a mint As the extracellular domain of PTK7 (exPTK7) was precipitated by Wnt3a and Wnt8 (immunoprecipitation; Supplementary Figure S2), it is sufficient for interaction with Wnt and, therefore, likely required for the PTK7-mediated inhibition of Wnt signalling. fzd7_a-ptk7 2 PTK7 6 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Fz7 10 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f4e mint fzd7_a-wnt3a 2 Wnt3a 13 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell Fz7 10 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f4f mint we asked if the PTK7 Wnt interaction requires the presence of Fz7. Cell supernatants of HEK293 cells secreting an HA-tagged construct consisting of the extracellular domain of PTK7 (sPTK7), or a myc-tagged construct comprising the extracellular Fz7 domain (sFz7) (Winklbauer et al, 2001), were incubated in combination with recombinant Wnt3a protein. The ability of HA-tagged PTK7 to co-precipitate recombinant Wnt3a was tested in the presence or absence of myc-tagged Fz7. Interestingly, sPTK7 could only precipitate Wnt3a in the presence of sFz7 (Figure 4F). ptk7-wnt3a-1 2 Wnt3a 13 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell PTK7 5 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association f4f mint we asked if the PTK7 Wnt interaction requires the presence of Fz7. Cell supernatants of HEK293 cells secreting an HA-tagged construct consisting of the extracellular domain of PTK7 (sPTK7), or a myc-tagged construct comprising the extracellular Fz7 domain (sFz7) (Winklbauer et al, 2001), were incubated in combination with recombinant Wnt3a protein. The ability of HA-tagged PTK7 to co-precipitate recombinant Wnt3a was tested in the presence or absence of myc-tagged Fz7. Interestingly, sPTK7 could only precipitate Wnt3a in the presence of sFz7 (Figure 4F).