IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Identification of cytochrome P450 2C2 protein complexes in mouse liver. Identification of cytochrome P450 2C2 protein complexes in mouse liver. Proteomics (1615-9853) 2011 imex curation Li B., Yau P., Kemper B. Apoptosis - Interactions involving proteins with a function related to apoptosis CYP2D1 has been entered as CYP2D10:- the authors confirm that the antibody used to detect Mouse CYP2D10 was anti-Rat CYP2D1 (Millipore), as this antibody cross reacts with mouse CYP2D10 ( proteins have ~85% homology). byronkem@illinois.edu 23-Sept-2011: Contacted by JYOTI. orchard Only protein-protein interactions mouse-liver Mus musculus liver liver anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Li B., Yau P., Kemper B. Apoptosis - Interactions involving proteins with a function related to apoptosis CYP2D1 has been entered as CYP2D10:- the authors confirm that the antibody used to detect Mouse CYP2D10 was anti-Rat CYP2D1 (Millipore), as this antibody cross reacts with mouse CYP2D10 ( proteins have ~85% homology). CYP2D1 has been entered as CYP2D10:- the authors confirm that the antibody used to detect Mouse CYP2D10 was anti-Rat CYP2D1 (Millipore), as this antibody cross reacts with mouse CYP2D10 ( proteins have ~85% homology). The authors confirm that the protein "PRD" is CPR- cytochrome P450 reductase. byronkem@illinois.edu Accepted 2011-AUG-30 by ORCHARD 23-Sept-2011: Contacted by JYOTI. Proteomics (1615-9853) 2011 orchard Only protein-protein interactions imex curation I think I have used the column as you suggest, this time.. Identification of cytochrome P450 2C2 protein complexes in mouse liver. Identification of cytochrome P450 2C2 protein complexes in mouse liver. Proteomics (1615-9853) 2011 imex curation Li B., Yau P., Kemper B. Apoptosis - Interactions involving proteins with a function related to apoptosis CYP2D1 has been entered as CYP2D10:- the authors confirm that the antibody used to detect Mouse CYP2D10 was anti-Rat CYP2D1 (Millipore), as this antibody cross reacts with mouse CYP2D10 ( proteins have ~85% homology). byronkem@illinois.edu 23-Sept-2011: Contacted by JYOTI. orchard Only protein-protein interactions mouse-liver Mus musculus liver liver density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Li B., Yau P., Kemper B. Apoptosis - Interactions involving proteins with a function related to apoptosis CYP2D1 has been entered as CYP2D10:- the authors confirm that the antibody used to detect Mouse CYP2D10 was anti-Rat CYP2D1 (Millipore), as this antibody cross reacts with mouse CYP2D10 ( proteins have ~85% homology). Different distribution patterns at higher molecular weights were observed for different proteins, indicating multiple complexes. The authors have a limited idea of what is colocalising/reacting with what - so I have entered all the reactants shown on the image together as "colocalisation" (really should be 'partial colocalisation') - as I would hesitate to put reactants into distinct reacting groups on the basis of the info given here - maybe this Expt should be deleted entirely? Mouse liver M2 affinity-purified protein complexes were analyzed in step sucrose gradients from 5% to 45% sucrose. byronkem@illinois.edu Accepted 2011-AUG-30 by ORCHARD 23-Sept-2011: Contacted by JYOTI. Proteomics (1615-9853) 2011 orchard Only protein-protein interactions imex curation I think I have used the column as you suggest, this time.. Identification of cytochrome P450 2C2 protein complexes in mouse liver. Identification of cytochrome P450 2C2 protein complexes in mouse liver. Proteomics (1615-9853) 2011 imex curation Li B., Yau P., Kemper B. Apoptosis - Interactions involving proteins with a function related to apoptosis CYP2D1 has been entered as CYP2D10:- the authors confirm that the antibody used to detect Mouse CYP2D10 was anti-Rat CYP2D1 (Millipore), as this antibody cross reacts with mouse CYP2D10 ( proteins have ~85% homology). byronkem@illinois.edu 23-Sept-2011: Contacted by JYOTI. orchard Only protein-protein interactions mouse-liver Mus musculus liver liver anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Li B., Yau P., Kemper B. Apoptosis - Interactions involving proteins with a function related to apoptosis CYP2D1 has been entered as CYP2D10:- the authors confirm that the antibody used to detect Mouse CYP2D10 was anti-Rat CYP2D1 (Millipore), as this antibody cross reacts with mouse CYP2D10 ( proteins have ~85% homology). Purified was by affinity to M2 agarose (Flag) and then further purification by affinity to Ni-NTA (Flag+His). The authors confirm that GI:1576133 should have been GI:576133 (GST Pi) and GI:20071598 should have been GI:20071589 (Bdh1). byronkem@illinois.edu Accepted 2011-AUG-30 by ORCHARD 23-Sept-2011: Contacted by JYOTI. Proteomics (1615-9853) 2011 orchard Only protein-protein interactions imex curation I think I have used the column as you suggest, this time.. Identification of cytochrome P450 2C2 protein complexes in mouse liver. Identification of cytochrome P450 2C2 protein complexes in mouse liver. Proteomics (1615-9853) 2011 imex curation Li B., Yau P., Kemper B. Apoptosis - Interactions involving proteins with a function related to apoptosis CYP2D1 has been entered as CYP2D10:- the authors confirm that the antibody used to detect Mouse CYP2D10 was anti-Rat CYP2D1 (Millipore), as this antibody cross reacts with mouse CYP2D10 ( proteins have ~85% homology). byronkem@illinois.edu 23-Sept-2011: Contacted by JYOTI. orchard Only protein-protein interactions mouse-liver Mus musculus liver liver anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Li B., Yau P., Kemper B. Apoptosis - Interactions involving proteins with a function related to apoptosis CYP2D1 has been entered as CYP2D10:- the authors confirm that the antibody used to detect Mouse CYP2D10 was anti-Rat CYP2D1 (Millipore), as this antibody cross reacts with mouse CYP2D10 ( proteins have ~85% homology). byronkem@illinois.edu Accepted 2011-AUG-30 by ORCHARD 23-Sept-2011: Contacted by JYOTI. Proteomics (1615-9853) 2011 orchard Only protein-protein interactions imex curation I think I have used the column as you suggest, this time.. q3unw2_mouse Cytochrome P450 Cyp2d9 protein protein mouse Mus musculus Mouse MELLTGTDLWPVAIFTVIFILLVDLTHQRQRWTSRYPPGPVPWPVLGNLLQVDLGNMPYSLYKLQNRYGDVFSLQMAWKPMVVINGLKAMKEMLLTCGEDTADRPPVPIFEYLGVKPGSQGVVLAPYGPEWREQRRFSVSTLRNFGLGKKSLEDWVTKEANHLCDAFTAQAGQPINPNPMLNKSTCNVIASLIFARRFEYEDPFLIRMLKVLEQSLTEVSGLIPEVLNAFPILLRIPRLADKALQGQKSFIAILDNLLTENRTTWDPVQAPRNLTDAFLAEIEKAKGNPESSFNDENLLMVVRDLFGAGMLTTSTTLSWALVLMILHPDVQRRVQQEIDEVIGQVRHPEMADQAHMPYTNAVIHEVQRFGDIVPVNLPRITSHDIEVQDFLIPKGTILLPNMSSMLKDESVWEKPLRFHPEHFLDAQGHFVKPEAFMPFSAGRRSCLGEALARMELFLFFTCLLQRFSFSVPDGQPQPSNSGVYGILVAPSPYQLCAVVRDQGH 628D647285F4B3B2 dhb13_mouse 17-beta-hydroxysteroid dehydrogenase 13 Hsd17b13 Alcohol dehydrogenase PAN1B-like Scdr9 Short-chain dehydrogenase/reductase 9 protein protein mouse Mus musculus Mouse MNLILEFLLLVGVIIYSYLESLVKFFIPRRRKSVTGQTVLITGAGHGIGRLTAYEFAKQKSRLVLWDINKRGVEETADKCRKLGAVVHVFVVDCSNRAEIYNSVDQVKREVGDVEIVVNNAGAIYPADLLSAKDEEITKTFEVNILGHFWIIKALLPSMLRRNSGHIVTVASVCGHGVIPYLIPYCSSKFAAVGFHRALTAELDTLGKTGIQTSCLCPVFVNTGFTKNPSTRLWPVLEPEEVARSLINGILTNKKMIFVPSYINISLILEKGPGFSSKHPHGGSQQPVTPIPGDLTPSSDFLKH 91D3A09C56024F01 cp2dq_mouse Cytochrome P450 2D26 Cyp2d26 Cyp2d-26 CYPIID26 protein protein mouse Mus musculus Mouse MGLLVGDDLWAVVIFTAIFLLLVDLVHRRQRWTACYPPGPVPFPGLGNLLQVDFENIPYSFYKLQNRYGNVFSLQMAWKPVVVVNGLKAVRELLVTYGEDTSDRPLMPIYNHIGYGHKSKGVILAPYGPEWREQRRFSVSTLRDFGLGKKSLEQWVTEEAGHLCDAFTKEAEHPFNPSPLLSKAVSNVIASLIYARRFEYEDPFFNRMLKTLKESLGEDTGFVGEVLNAIPMLLHIPGLPDKAFPKLNSFIALVNKMLIEHDLTWDPAQPPRDLTDAFLAEVEKAKGNPESSFNDKNLRIVVIDLFMAGMVTTSTTLSWALLLMILHPDVQRRVHQEIDEVIGHVRHPEMADQARMPYTNAVIHEVQRFADIVPTNLPHMTSRDIKFQDFFIPKGTTLIPNLSSVLKDETVWEKPLRFYPEHFLDAQGHFVKHEAFMPFSAGRRSCLGEPLARMELFLFFTCLLQRFSFSVPDGQPRPSDYGIYTMPVTPEPYQLCAVAR B3DDCD88DFA3F265 bdh_mouse D-beta-hydroxybutyrate dehydrogenase, mitochondrial 3-hydroxybutyrate dehydrogenase Bdh1 Bdh protein protein mouse Mus musculus Mouse MLAARLSRPLSQLPGKALSVRDRENGTRHTLLFYPASFSPDTRRTYASQADAASGKAILITGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGDAGVKELDSLKSDRLRTIQLNVCNSEEVEKAVETIRSGLKDPEKGMWGLVNNAGISTFGEVEFTSMETYKEVAEVNLWGTVRTTKSFLPLLRRAKGRVVNISSMLGRMANPARSPYCITKFGIEAFSDCLRYEMHPLGVKVSVVEPGNFIAATSLYSPERIQAIAKKMWDDLPEVVRKDYGRKYFDEKIAKMETYCNSGSTDTSSVINAVTHALTAATPYTRYHPMDYYWWLRMQIMTHFPGAISDKIYIH B72B0550713EDB7F met7b_mouse Thiol S-methyltransferase METTL7B Mettl7b Methyltransferase-like protein 7B protein protein mouse Mus musculus Mouse MDALVLFLQLLVLLLTLPLHLLALLGCWQPICKTYFPYFMAMLTARSYKKMESKKRELFSQIKDLKGTSGNVALLELGCGTGANFQFYPQGCKVTCVDPNPNFEKFLTKSMAENRHLQYERFIVAYGENMKQLADSSMDVVVCTLVLCSVQSPRKVLQEVQRVLRPGGLLFFWEHVAEPQGSRAFLWQRVLEPTWKHIGDGCHLTRETWKDIERAQFSEVQLEWQPPPFRWLPVGPHIMGKAVK 1E760F4BB0004637 cp2da_mouse Cytochrome P450 2D10 Cyp2d10 Cyp2d-10 CYPIID10 Cytochrome P450-16-alpha Cytochrome P450CB Testosterone 16-alpha hydroxylase protein protein mouse Mus musculus Mouse MELLTGAGLWSVAIFTVIFILLVDLMHRHQRWTSRYPPGPVPWPVLGNLLQVDLDNMPYSLYKLQNRYGDVFSLQMGWKPMVVINGLKAMKEVLLTCGEDTADRPQVPIFEYLGVKPGSQGVVLAPYGPEWREQRRFSVSTLRNFGLGKKSLEDWVTKEARHLCDAFTAQAGQPINPNTMLNNAVCNVIASLIFARRFEYEDPYLIRMQKVLEDSLTEISGLIPEVLNMFPILLRIPGLPGKVFQGQKSLLAIVENLLTENRNTWDPDQPPRNLTDAFLAEIEKVKGNAESSFNDENLRMVVLDLFTAGMVTTSTTLSWALLLMILHPDVQRRVQQEIDAVIGQVRHPEMADQARMPYTNAVIHEVQRFGDIAPLNLPRITSRDIEVQDFLIPKGSILIPNMSSVLKDETVWEKPLRFHPEHFLDAQGHFVKPEAFMPFSAGRRSCLGEPLARMELFLFFTCLLQHFSFSVPNGQPRPRNLGVFPFPVAPYPYQLCAVMREQGH 41375F9834489401 q811m6_mouse Igtp protein protein mouse Mus musculus Mouse MDLVTKLPQNIWKTFTLFINMANYLKRLISPWSKSMTAGESLYSSQNSSSPEVIEDIGKAVTEGNLQKVIGIVKDEIQSKSRYRVKIAVTGDSGNGMSSFINALRFIGHEEEDSAPTGVVRTTKKPACYSSDSHFPYVELWDLPGLGATAQSVESYLEEMQISTYHLIIIVASEQFSSNHVKLAITMQRMRKRFYVVWTKLDRDLSTSTFPEPQLLQSIQRNIRENLQQAQVRDPPLFLISCFSPSFHDFPELRNTLQKDIFSIRYRDPLEIISQVCDKCISNKAFSLKEDQMLMKDLEAAVSSEDDTANLERGLQTYQKVFGVDDGSLQQVARSTGRLEMGSRALQFQDLIKMDRRLELMMCFAVNKFLRVLESSWWYGLWNVVTRYFRHQRHKLVIEIVAENTKTSLRKALKDSVLPPEIH 1094233681D25B8C hyep_mouse Epoxide hydrolase 1 Ephx1 Epoxide hydratase Microsomal epoxide hydrolase protein protein mouse Mus musculus Mouse MWLELILASVLGFVIYWFVSRDKEETLPLEDGWWGPGSKPSAKEDESIRPFKVETSDEEIKDLHQRIDRFRASPPLEGSRFHYGFNSSYLKKVVSFWRNEFDWRKQVEILNQYPHFKTKIEGLDIHFIHVKPPQLPSGRTPKPLLMVHGWPGSFYEFYKIIPLLTDPKTHGLSDEHVFEVICPSIPGYGFSEASSKKGLNSVATARIFYKLMSRLGFQKFYIQGGDWGSLICTNIAQMVPNHVKGLHLNMSFISRNIYSLTPLLGQRFGRFLGYTEKDLELLYPFKEKVFYNIMRESGYLHIQATKPDTVGCALNDSPVGLAAYILEKFSTWTKSEYRELEDGGLERKFSLEDLLTNIMIYWTTGTIVSSQRFYKENLGQGVMVHRHEGMKVFVPTGYSAFPSEILHAPEKWVKVKYPKLISYSYMERGGHFAAFEEPKLLAQDIRKFVSLAELQ 1567F619D9114C58 rdh7_mouse Retinol dehydrogenase 7 Rdh7 Crad2 Cis-retinol/3alpha-hydroxysterol short-chain dehydrogenase isozyme 2 Cis-retinol/androgen dehydrogenase type 2 protein protein mouse Mus musculus Mouse MWLYLVALVGLWTLLRFFRERQVVSHLQDKYVFITGCDSGFGNLLARQLDRRGMRVLAACLTEKGAEQLRNKTSDRLETVILDVTKTESIVAATQWVKERVGNRGLWGLVNNAGICVFAINEWLKKEDFANILDVNLLGMIEVTLSMLPLVRKARGRVVNISSSMGRVSLCGGGYCISKYGVEAFSDSLRREISYFGVKVAIIEPGGFRTNVSNYERLSHSIEKLWDQTSSEVKEVYDKNFLDSYIKAIQSLTDTCSDDLSVVTDCMEHALTACHPRTRYSAGWDAKLFYLPLSYMPTFLVDAMLYWSSVKPAQAL 0BFB9A1C2DDE3C17 pgrc1_mouse Membrane-associated progesterone receptor component 1 Pgrmc1 Pgrmc protein protein mouse Mus musculus Mouse MAAEDVVATGADPSELEGGGLLHEIFTSPLNLLLLGLCIFLLYKIVRGDQPGASGDNDDDEPPPLPRLKRRDFTPAELRRFDGVQDPRILMAINGKVFDVTKGRKFYGPEGPYGVFAGRDASRGLATFCLDKEALKDEYDDLSDLTPAQQETLSDWDSQFTFKYHHVGKLLKEGEEPTVYSDDEEPKDETARKNE 1FFD17AFAE6C81FC bap31_mouse B-cell receptor-associated protein 31 Bcap31 Bap31 p28 protein protein mouse Mus musculus Mouse MSLQWTTVATFLYAEVFAVLLLCIPFISPKRWQKVFKSRLVELVVTYGNTFFVVLIVILVLLVIDAVREILKYDDVTEKVNLQNNPGAMEHFHMKLFRAQRNLYIAGFSLLLSFLLRRLVTLISQQATLLASNEAFKKQAESASEAAKKYMEENDQLKKGAAEDGDKLDIGNTEMKLEENKSLKNDLRKLKDELASTKKKLEKAENEALAMQKQSEGLTKEYDRLLEEHAKLQASVRGPSVKKEE A8C53B7A04E06C97 cp2c2_rabit Cytochrome P450 2C2 CYP2C2 CYPIIC2 Cytochrome P450 PBc2 Cytochrome P450 PHP2 Laurate omega-1 hydroxylase protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MDLVVVLGLCLSCLLLPSLWKQSHGGGKLPPGPTPFPILGNVLQLDFKDLSKSLTNLSKVYGPVFTVYLGMKPTVVVHGYEAVKEALVDLGHELSGRSRFLVTAKLNKGFGVIFSNGKRWTETRRFSLMTLRNFGMGKRSIEERVQEEAHCLVEELRKTNASPCDPTFILGAAPCNVICSVIFQNRFDYTDQDFLSLMGKFNENFKILNSPWVQFCNCFPILFDYFPGSHRKAVKNIFYVKNYITEQIKEHQKSLDINNPRDFIDCFLIKMEQEKCNQQSEFTIENLLTTVSDVFMAGTETTSTTLRYGLLLLMKHPEVIAKVQEEIERVIGRHRSPCMQDRSRMPYTDATVHEIQRYINLIPNNVPHTTICNLKFRNYLIPKGTDVLTSLSSVLHDDKEFPNPDRFDPGHFLDASGNFRKSDYFMPFSTGKRVCVGEALARMELFLFLTAILQNFTPKPLVNPNNVDENPFSSGIVRVPPLYRVSFIPV C105A6C6F3687E23 ud19_mouse UDP-glucuronosyltransferase 1A9 Ugt1a9 Ugt1 Ugt1a12 UDP-glucuronosyltransferase 1-7 UGT1A12 UGTP4 UDP-glucuronosyltransferase 1-9 protein protein mouse Mus musculus Mouse MAPVAFPTSFFLCLLLASGLAQAGRLLVVPMDGSHWFTMQMVVEKLIHRGHEVVVVIPEVSWQLGKSLNCTVKTYSISHTLEDLDREFKYLSYTQWKTPEHSIRSFLTGSARGFFELTFSHCRSLFNDKKLVEYLKQRFFDAVFLDPFDVCGLIVAKYFSLPSVIFARGVFCDYLEEGAQCPSLPSYVPRLFSKYTDTMTFKERVWNHLIYIEEHAFCSYFLRTAVEVASEILQTPVTMTDLFSPVSIWLLRTDFVLEFPRPVMPNMVFIGGINCLQKKSLSKEFEAYVNASGEHGIVVFSLGSMVSEIPEKKAMEIAEALGRIPQTVLWRYTGTRPSNLAKNTILVKWLPQNDLLGHPKTRAFITHSGSHGIYEGICNGVPMVMMPLFGDQMDNAKRMETRGAGVTLNVLEMTADDLENALKTVINNKSYKENIMRLSSLHKDRPIEPLDLAVFWVEYVMRHKGAPHLRPAAHDLTWYQYHSLDVIGFLLAIVLTVVFIVFKCCAYGCRKCFGGKGRVKKSHKSKTH B051E4F510EADCB4 gstp1_mouse Glutathione S-transferase P 1 Gstp1 Gstpib GST YF-YF GST-piB GST class-pi Preadipocyte growth factor protein protein mouse Mus musculus Mouse MPPYTIVYFPVRGRCEAMRMLLADQGQSWKEEVVTIDTWMQGLLKPTCLYGQLPKFEDGDLTLYQSNAILRHLGRSLGLYGKNQREAAQMDMVNDGVEDLRGKYVTLIYTNYENGKNDYVKALPGHLKPFETLLSQNQGGKAFIVGDQISFADYNLLDLLLIHQVLAPGCLDNFPLLSAYVARLSARPKIKAFLSSPEHVNRPINGNGKQ DAC7762F4E34FF27 cp3ab_mouse Cytochrome P450 3A11 Cyp3a11 Cyp3a-11 CYPIIIA11 Cytochrome P-450IIIAM1 Cytochrome P-450UT protein protein mouse Mus musculus Mouse MDLVSALSLETWVLLAISLVLLYRYGTRKHELFKKQGIPGPKPLPFLGTVLNYYKGLWKFDMECYKKYGKTWGLFDGQTPLLAVTDPETIKNVLVKECFSVFTNRRDFGPVGIMSKAISISKDDEWKRYRALLSPTFTSGKLKEMFPVIEQYGDILVKYLRQKAKKGKPVTMKDVLGAYSMDVITSTSFGVNVDSLNNPEDPFVEKAKKLLRFDFFDPLLFSVVLFPFLTPVYEMLNICMFPKDSIEFFKKFVDRMKESRLDSKQKHRVDFLQLMMNSHNNSKDKVSHKALSDMEITAQSIIFIFAGYETTSSTLSFTLHSLATHPDIQKKLQDEIDEALPNKAPPTYDTVMEMEYLDMVLNETLRLYPIANRLERVCKKDVELNGVYIPKGSTVMIPSYALHHDPQHWSEPEEFQPERFSKENKGSIDPYVYLPFGNGPRNCLGMRFALMNMKLALTKIMQNFSFQPCKETQIPLKLSRQGLLQPEKPIVLKVVPRDAVITGA E369AF71CE23F180 ncpr_mouse NADPH--cytochrome P450 reductase Por protein protein mouse Mus musculus Mouse MGDSHEDTSATVPEAVAEEVSLFSTTDIVLFSLIVGVLTYWFIFKKKKEEIPEFSKIQTTAPPVKESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEEYDLADLSSLPEIDKSLVVFCMATYGEGDPTDNAQDFYDWLQETDVDLTGVKFAVFGLGNKTYEHFNAMGKYVDQRLEQLGAQRIFELGLGDDDGNLEEDFITWREQFWPAVCEFFGVEATGEESSIRQYELVVHEDMDTAKVYTGEMGRLKSYENQKPPFDAKNPFLAAVTTNRKLNQGTERHLMHLELDISDSKIRYESGDHVAVYPANDSTLVNQIGEILGADLDVIMSLNNLDEESNKKHPFPCPTTYRTALTYYLDITNPPRTNVLYELAQYASEPSEQEHLHKMASSSGEGKELYLSWVVEARRHILAILQDYPSLRPPIDHLCELLPRLQARYYSIASSSKVHPNSVHICAVAVEYEAKSGRVNKGVATSWLRTKEPAGENGRRALVPMFVRKSQFRLPFKPTTPVIMVGPGTGVAPFMGFIQERAWLREQGKEVGETLLYYGCRRSDEDYLYREELARFHKDGALTQLNVAFSREQAHKVYVQHLLKRDKEHLWKLIHEGGAHIYVCGDARNMAKDVQNTFYDIVAEFGPMEHTQAVDYVKKLMTKGRYSLDVWS 060282A7A55483AF hsp7c_mouse Heat shock cognate 71 kDa protein Heat shock 70 kDa protein 8 Hspa8 Hsc70 Hsc73 protein protein mouse Mus musculus Mouse MSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIISWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD DIP protein P63017 original sequence version: 76 DIP protein P63017 original sequence version: 118 03A27B30E6C076ED dhi1_mouse Corticosteroid 11-beta-dehydrogenase isozyme 1 Hsd11b1 Hsd11 11-beta-hydroxysteroid dehydrogenase 1 11beta-HSD1A protein protein mouse Mus musculus Mouse MAVMKNYLLPILVLFLAYYYYSTNEEFRPEMLQGKKVIVTGASKGIGREMAYHLSKMGAHVVLTARSEEGLQKVVSRCLELGAASAHYIAGTMEDMTFAEQFIVKAGKLMGGLDMLILNHITQTSLSLFHDDIHSVRRVMEVNFLSYVVMSTAALPMLKQSNGSIAVISSLAGKMTQPMIAPYSASKFALDGFFSTIRTELYITKVNVSITLCVLGLIDTETAMKEISGIINAQASPKEECALEIIKGTALRKSEVYYDKSPLTPILLGNPGRKIMEFFSLRYYNKDMFVSN ADE42B11D82DD6CD ktn1_mouse Kinectin Ktn1 protein protein mouse Mus musculus Mouse MELYESTYFIVLIPSVVITVIFLFFWLFMKETLYDEVLAKQKREQKLISTKTDKKKAEKKKNKKKEIQNGTLRESDSEHVPRDFKLSDASPAEDEQFVPAPLNVAETSSSVRERQKKEKKQKPSLEEQVIKESDASKIPGKKVEPVLVTKQPAPPPPLEAAALKKKAGQKKSKNGSEEQDKKVEMLMAPSKEQDVLLSHQDTKQEGGLGKKKGLSKKQKSENVAVLVDEPLIHATTYMPLDNANSNLMMDKREIIDMIKPDHVEGIQKSGTKKLKIETDKENAEVKFKDFLLSLKTMMFSEDEALCVVDLLKEKSGVIKEALKKSNKGELSGLLHQLQEKERLLSAMKEDAAASKERCKRLTQEMMTEKERSSVVIARMKDRIGTLEKEHNIFQNKMHASYQETQQMQMKFQQVQEQMEAEIAHLKQENGILRDAVSNTTNQLESKQSAELNKLRQDCGRLVSELNEKTGKLQQEGVQKKNAEQAATQLKVQLQEAERRWEEVQSYIRKRTAEHEAAQQDLQSKFVAKENEVQSLHSKLTDTLVSKQQLEQRLMQLMESEQKRASKEESLQIQVQDILEQNEALKAQIQQFHSQIAAQTSASVLAEELHKVIAEKDKQLKQTEDSLANEQDHLASKEEELKDVQNMNFLLKAEVQKWQALANEQAATAHEVEKMQKSIHVKEDEIRLLEEQLQHEVASKMEELKILSEQNKALQSEVRKLQTAVSQQPNKDVVEQMEKCIQEKDEKLRTVEELLETGLIQVATREEELSAIRTENSTLTREVQELKAKQSDQVSFVSLIEDLKRVIHEKDGQIKSVEELLEVELLKVANKEKTVQALKQEIEVLKEEIGNAQLEKAHQLSVTSQVQELQNLLRGKEEQVNSMKAALEDRDRGLTGRGTCAQVCSTPQFEELESVLKEKDNEIKRIEVKLKDTESDVSKMSELLKEVQEENKFLKCQLSHQKHQQASFPSQEELQTVISEKEKEITDLCNELESLKNAVEHQRKKNNDLREKNWEAMEALASTEKMLQDRVNKTSKERRQHVEAIELESKDLLKRLFPTVSVPSNLNYSEWLRGFEKKAKACVAGTSDAEAVKVLEHRLKEASEMHTLLQLECEKYKSVLAETEGILQKLQRSVEQEESKWKIKADESQRMIKQMQSSFTASERELERLRQENKDMENLRREREHLEMELEKAEMERSTYVMEVRELKDLLTELQKKLDDSYSEAVRQNEELNLLKTQLNETHSKLQNEQTERKKVADDLHKAQQSLNSIHSKISLKAAGDTVVIENSDISPEMESPEKETMSVSLNQTVTQLQQLLQEVNQQLTKET 2B79EB332D2470E5 acsl1_mouse Long-chain-fatty-acid--CoA ligase 1 Acsl1 Acsl2 Facl2 Long-chain acyl-CoA synthetase 1 Arachidonate--CoA ligase Phytanate--CoA ligase protein protein mouse Mus musculus Mouse MEVHELFRYFRMPELIDIRQYVRTLPTNTLMGFGAFAALTTFWYATRPKALKPPCDLSMQSVEIAGTTDGIRRSAVLEDDKLLVYYYDDVRTMYDGFQRGIQVSNNGPCLGSRKPNQPYEWISYKEVAELAECIGSGLIQKGFKPCSEQFIGLFSQNRPEWVIVEQGCFSYSMVVVPLYDTLGADAITYIVNKAELSVIFADKPEKAKLLLEGVENKLTPCLKIIVIMDSYGSDLVERGKKCGVEIISLKALEDLGRVNRVKPKPPEPEDLAIICFTSGTTGNPKGAMITHQNIINDCSGFIKATESAFIASTDDVLISFLPLAHMFETVVECVMLCHGAKIGFFQGDIRLLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQANTSLKRWLLDFASKRKEAELRSGIVRNNSLWDKLIFHKIQSSLGGKVRLMITGAAPVSATVLTFLRTALGCQFYEGYGQTECTAGCCLSLPGDWTAGHVGAPMPCNYVKLVDVEEMNYLASKGEGEVCVKGANVFKGYLKDPARTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENIYLRSEAVAQVFVHGESLQAFLIAVVVPDVESLPSWAQKRGLQGSFEELCRNKDINKAILDDLLKLGKEAGLKPFEQVKGIAVHPELFSIDNGLLTPTLKAKRPELRNYFRSQIDELYATIKI C8319972A764CCFA rtn3_mouse Reticulon-3 Rtn3 protein protein mouse Mus musculus Mouse MAESSAATQSPSVSSSSSGAEPSALGGGGGSPGACPALGAKSCGSSCADSFVSSSSSQPVSIFSTSQAGLSSLCSDEPPSKSMTSSFLSSSEIHNPDPTTPLGEKSETLGSQFVLAKGKDPLVLLDKKKLDSPQGTNKDRVDAPVSLATGIPCSHPSIPDSFPEQPAFLSKEIGPAEEWVVKDQEPKNPNKVPDGEDRSALDFGQSKAEHICTYSLSPSELPVASVEKDSPESPFEVIIDKATFDREFKDLYKENPNDLGGWAAHGDRESPADLLEMNDKLFPLRNKEAGRYPSSVLLGRQFSHTTAALEEVSRCVNDMHNFTNEILTWDLDPQAKQQANKTSCTTESTGLDRSELRSEIPVINLKTNPQQKMPVCSFNGSTPITKSTGDWTEAFTEGKPVRDYLSSTKEAGGNGVPGSSQLHSELPGSMPEKWVSGSGAATVEVTLPNLRGAWPNSVMGEVTEVDSSGESDDTVIEDITEKPDSLPSAAAKTSEREIKETPSRETVRSEMCENSEQPQAQPETPTQKSLEGEVASQVPNTLNEVTPEKLDMTNNPKVCSAAPPSVLNETGFSLTVPASAKLESLLGKYVEDTDGSSPEDLMAVLTGAEEKGIVDKEEGDVLEAVLEKIADFKNTLPVELLHESELSGSETKNIKSKYSEDSRETTGGAPTMSPDLEQEQLTIRAIKELGERQAEKVQDEGISSGGKLKQTFAPQSGPQSSSDILEHTDVKTGSDLGIPKNPTIIKNTRIDSISSLTKTEMVNKNVLARLLSDFPVHDLIFWRDVKKTGFVFGTTLIMLLSLAAFSVISVVSYLILALLSVTISFRVYKSVIQAVQKSEEGHPFKAYLDVDITLSSEAFHNYMNAAMVHVNKALKLIIRLFLVEDLVDSLKLAVFMWLMTYVGAVFNGITLLILAELLVFSVPIVYEKYKTQIDHYVGIARDQTKSIVEKIQAKLPGIAKKKAE 5170809A696F8F7A apoe_mouse Apolipoprotein E Apoe protein protein mouse Mus musculus Mouse MKALWAVLLVTLLTGCLAEGEPEVTDQLEWQSNQPWEQALNRFWDYLRWVQTLSDQVQEELQSSQVTQELTALMEDTMTEVKAYKKELEEQLGPVAEETRARLGKEVQAAQARLGADMEDLRNRLGQYRNEVHTMLGQSTEEIRARLSTHLRKMRKRLMRDAEDLQKRLAVYKAGAREGAERGVSAIRERLGPLVEQGRQRTANLGAGAAQPLRDRAQAFGDRIRGRLEEVGNQARDRLEEVREHMEEVRSKMEEQTQQIRLQAEIFQARLKGWFEPIVEDMHRQWANLMEKIQASVATNPIITPVAQENQ 3B36FA897CC34170 fmo5_mouse Flavin-containing monooxygenase 5 Fmo5 Dimethylaniline oxidase 5 Hepatic flavin-containing monooxygenase 5 Dimethylaniline monooxygenase [N-oxide-forming] 5 NAPDH oxidase protein protein mouse Mus musculus Mouse MAKKRIAVIGAGASGLTCIKCCLEEGLEPVCFERSGDIGGLWRFQEAPEEGRASIYQSVVINTSKEMMCFSDYPIPDHYPNYMHNSQVLEYFRMYAKEFDLLKYIQFKTTVCSVKKQPDFSTSGQWQVVTECEGKQQVDVFDGVLVCTGHHTDAHLPLESFPGIEKFKGKYFHSRDYKNPVEFTGKRVIVIGIGNSGGDLAVEISHTAKQVFLSTRRGAWILNRVGKHGYPIDLLLSSRIMYYLSRICGPSLKNNYMEKQMNQRFDHEMFGLKPKHRALSQHPTVNDDLPNRIIAGLVKVKGNVKEFTETAAVFEDGSREDGIDVVIFATGYSFAFPFLEDSVKVVKNKVSLYKKVFPPNLEKPTLAIIGLIQPLGAIMPISELQGRWATQVFKGLKKLPSQSEMMAEINKAREEMAKRYVDSQRHTIQGDYIDTMEEIADLVGVRPNILPLVFTDPRLALRLLLGPCTPVQYRLQGPGKWAGARKTILTTEDRVRKPLMTRVVERDSSGGSLVTVRVLMLAVAFFAVILAYF 4B9B246C4D7EBE34 bip_mouse Endoplasmic reticulum chaperone BiP Immunoglobulin heavy chain-binding protein Hspa5 Grp78 78 kDa glucose-regulated protein Binding-immunoglobulin protein Heat shock protein 70 family protein 5 Heat shock protein family A member 5 protein protein mouse Mus musculus Mouse MMKFTVVAAALLLLGAVRAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSGGPPPTGEEDTSEKDEL DIP protein P20029 original sequence version: 154 DIP protein P20029 original sequence version: 113 DIP protein P20029 original sequence version: 112 DIP protein P20029 original sequence version: 117 DIP protein P20029 original sequence version: 165 AFB795D15E20FAC2 nb5r3_mouse NADH-cytochrome b5 reductase 3 Cyb5r3 Dia1 Diaphorase-1 protein protein mouse Mus musculus Mouse MGAQLSTLSHVVLSPVWFIYSLFMKLFQRSTPAITLENPDIKYPLRLIDKEVISPDTRRFRFALPSPQHILGLPIGQHIYLSTRIDGNLVIRPYTPVSSDDDKGFVDLVVKVYFKDTHPKFPAGGKMSQYLENMKIGDTIEFRGPNGLLVYQGKGKFAIRADKKSNPVVRTVKSVGMIAGGTGITPMLQVIRAVLKDPNDHTVCYLLFANQSEKDILLRPELEELRNEHSARFKLWYTVDKAPDAWDYSQGFVNEEMIRDHLPTPGEEPLILMCGPPPMIQFACLPNLERVGHPKERCFTF DIP protein Q9DCN2 original sequence version: 98 984D5B73430F725D cyp2c2-acsl1-9 3 8 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey 21 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 28 unspecified role unspecified role prey prey 25 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey 19 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 26 unspecified role unspecified role prey prey 27 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey association association Fig 1, Table 1 , Supplementary Table S1. cyp2c2-acsl1-10 1 12 unspecified role unspecified role prey prey 27 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 19 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey association association Fig 2A cyp2c2-acsl1-7 2 14 unspecified role unspecified role neutral component neutral component 29 unspecified role unspecified role neutral component neutral component 16 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 27 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component 24 unspecified role unspecified role neutral component neutral component 19 unspecified role unspecified role neutral component neutral component 22 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component association association Fig 3 fmo5 4 19 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 27 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey association association Fig 4B The fraction of PGRMC1 and mEH coprecipitating from the M2-bound fractions was much greater than from the M2-unbound fraction. acsl1-hyep 4 14 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey association association Fig 4B-2 Coimmunoprecipitation with ACSL1 of PGRMC1 and mEH occurs, but not with 11beta-HSD1 in the M2-bound fraction. hsd1-dhi1 4 27 unspecified role unspecified role prey prey 22 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey association association Fig 4B-3 PGRMC1 selectively coimmunoprecipitated in the M2-bound fraction compared with the M2-unbound fraction, whereas FMO5 and Cyp3a11 did not coimmunoprecipitate efficiently in the M2-bound fraction. meh-hyep 4 10 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey association association Fig 4B-4 mEH coimmunoprecipitated in the M2-bound fraction with PGRMC1 and Cyp2d10, but not with Cyp3a11. cyp2d1 4 10 unspecified role unspecified role bait bait 22 unspecified role unspecified role prey prey association association Fig 4B-5