InnateDB InnateDB The orphan nuclear receptor SHP acts as a negative regulator in inflammatory signaling triggered by Toll-like receptors. The orphan nuclear receptor SHP acts as a negative regulator in inflammatory signaling triggered by Toll-like receptors. Nat. Immunol. (1529-2908) 2011 imex curation Yuk J.M., Shin D.M., Lee H.M., Kim J.J., Kim S.W., Jin H.S., Yang C.S., Park K.A., Chanda D., Kim D.K., Huang S.M., Lee S.K., Lee C.H., Kim J.M., Song C.H., Lee S.Y., Hur G.M., Moore D.D., Choi H.S., Jo E.K. hsc@chonnam.ac.kr 23-Sept-2011: Contacted by JYOTI. orchard Only protein-protein interactions Curated by: InnateDB - a knowledge resource for innate immunity human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yuk J.M., Shin D.M., Lee H.M., Kim J.J., Kim S.W., Jin H.S., Yang C.S., Park K.A., Chanda D., Kim D.K., Huang S.M., Lee S.K., Lee C.H., Kim J.M., Song C.H., Lee S.Y., Hur G.M., Moore D.D., Choi H.S., Jo E.K. hsc@chonnam.ac.kr Accepted 2011-SEP-12 by ORCHARD 23-Sept-2011: Contacted by JYOTI. Nat. Immunol. (1529-2908) 2011 orchard Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity The orphan nuclear receptor SHP acts as a negative regulator in inflammatory signaling triggered by Toll-like receptors. The orphan nuclear receptor SHP acts as a negative regulator in inflammatory signaling triggered by Toll-like receptors. Nat. Immunol. (1529-2908) 2011 imex curation Yuk J.M., Shin D.M., Lee H.M., Kim J.J., Kim S.W., Jin H.S., Yang C.S., Park K.A., Chanda D., Kim D.K., Huang S.M., Lee S.K., Lee C.H., Kim J.M., Song C.H., Lee S.Y., Hur G.M., Moore D.D., Choi H.S., Jo E.K. hsc@chonnam.ac.kr 23-Sept-2011: Contacted by JYOTI. orchard Only protein-protein interactions Curated by: InnateDB - a knowledge resource for innate immunity mouse-bmm mouse bone marrow derived macrophages bmm bone marrow derived macrophages Bone marrow derived macrophages (BMM) were generated in vitro from precursor cells harvested from the femurs of C57BL/6 mice (7�8 weeks) by proliferation and differentiation in the presence of L-cell conditioned medium. as a source of CSF-1 confocal microscopy confocal microscopy immunostaining immunostaining Yuk J.M., Shin D.M., Lee H.M., Kim J.J., Kim S.W., Jin H.S., Yang C.S., Park K.A., Chanda D., Kim D.K., Huang S.M., Lee S.K., Lee C.H., Kim J.M., Song C.H., Lee S.Y., Hur G.M., Moore D.D., Choi H.S., Jo E.K. hsc@chonnam.ac.kr Accepted 2011-SEP-12 by ORCHARD 23-Sept-2011: Contacted by JYOTI. Nat. Immunol. (1529-2908) 2011 orchard Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity The orphan nuclear receptor SHP acts as a negative regulator in inflammatory signaling triggered by Toll-like receptors. The orphan nuclear receptor SHP acts as a negative regulator in inflammatory signaling triggered by Toll-like receptors. Nat. Immunol. (1529-2908) 2011 imex curation Yuk J.M., Shin D.M., Lee H.M., Kim J.J., Kim S.W., Jin H.S., Yang C.S., Park K.A., Chanda D., Kim D.K., Huang S.M., Lee S.K., Lee C.H., Kim J.M., Song C.H., Lee S.Y., Hur G.M., Moore D.D., Choi H.S., Jo E.K. hsc@chonnam.ac.kr 23-Sept-2011: Contacted by JYOTI. orchard Only protein-protein interactions Curated by: InnateDB - a knowledge resource for innate immunity mouse-raw264_7 Mus musculus monocyte macrophage cell line RAW264 raw264_7 Mouse monocyte macrophage cellline RAW264.7 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yuk J.M., Shin D.M., Lee H.M., Kim J.J., Kim S.W., Jin H.S., Yang C.S., Park K.A., Chanda D., Kim D.K., Huang S.M., Lee S.K., Lee C.H., Kim J.M., Song C.H., Lee S.Y., Hur G.M., Moore D.D., Choi H.S., Jo E.K. hsc@chonnam.ac.kr Accepted 2011-SEP-12 by ORCHARD 23-Sept-2011: Contacted by JYOTI. Nat. Immunol. (1529-2908) 2011 orchard Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity The orphan nuclear receptor SHP acts as a negative regulator in inflammatory signaling triggered by Toll-like receptors. The orphan nuclear receptor SHP acts as a negative regulator in inflammatory signaling triggered by Toll-like receptors. Nat. Immunol. (1529-2908) 2011 imex curation Yuk J.M., Shin D.M., Lee H.M., Kim J.J., Kim S.W., Jin H.S., Yang C.S., Park K.A., Chanda D., Kim D.K., Huang S.M., Lee S.K., Lee C.H., Kim J.M., Song C.H., Lee S.Y., Hur G.M., Moore D.D., Choi H.S., Jo E.K. hsc@chonnam.ac.kr 23-Sept-2011: Contacted by JYOTI. orchard Only protein-protein interactions Curated by: InnateDB - a knowledge resource for innate immunity mouse-bmm mouse bone marrow derived macrophages bmm bone marrow derived macrophages Bone marrow derived macrophages (BMM) were generated in vitro from precursor cells harvested from the femurs of C57BL/6 mice (7�8 weeks) by proliferation and differentiation in the presence of L-cell conditioned medium. as a source of CSF-1 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yuk J.M., Shin D.M., Lee H.M., Kim J.J., Kim S.W., Jin H.S., Yang C.S., Park K.A., Chanda D., Kim D.K., Huang S.M., Lee S.K., Lee C.H., Kim J.M., Song C.H., Lee S.Y., Hur G.M., Moore D.D., Choi H.S., Jo E.K. hsc@chonnam.ac.kr Accepted 2011-SEP-12 by ORCHARD 23-Sept-2011: Contacted by JYOTI. Nat. Immunol. (1529-2908) 2011 orchard Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity nr0b2_mouse Nuclear receptor subfamily 0 group B member 2 Nr0b2 Shp Orphan nuclear receptor SHP Small heterodimer partner protein protein mouse Mus musculus Mouse MSSGQSGVCPCQGSAGRPTILYALLSPSPRTRPVAPASHSHCLCQQQRPVRLCAPHRTCREALDVLAKTVAFLRNLPSFCHLPHEDQRRLLECCWGPLFLLGLAQDAVTFEVAEAPVPSILKKILLEEASSGTQGAQPSDRPQPSLAAVQWLQRCLESFWSLELGPKEYAYLKGTILFNPDVPGLRASCHIAHLQQEAHWALCEVLEPWYPASQGRLARILLMASTLKNIPGTLLVDLFFRPIMGDVDITELLEDMLLLR 7B859325AA73DCA1 traf6_mouse TNF receptor-associated factor 6 RING-type E3 ubiquitin transferase TRAF6 Traf6 E3 ubiquitin-protein ligase TRAF6 protein protein mouse Mus musculus Mouse MSLLNCENSCGSSQSSSDCCAAMAASCSAAVKDDSVSGSASTGNLSSSFMEEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLTVKCPNKGCLQKMELRHLEDHQVHCEFALVNCPQCQRPFQKCQVNTHIIEDCPRRQVSCVNCAVSMAYEEKEIHDQSCPLANIICEYCGTILIREQMPNHYDLDCPTAPIPCTFSVFGCHEKMQRNHLARHLQENTQLHMRLLAQAVHNVNLALRPCDAASPSRGCRPEDPNYEETIKQLESRLVRQDHQIRELTAKMETQSMYVGELKRTIRTLEDKVAEMEAQQCNGIYIWKIGNFGMHLKSQEEERPVVIHSPGFYTGRPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEALIRQNHEEVMDAKPELLAFQRPTIPRNPKGFGYVTFMHLEALRQGTFIKDDTLLVRCEVSTRFDMGGLRKEGFQPRSTDAGV DIP protein P70196 original sequence version: 139 DIP protein P70196 original sequence version: 128 DIP protein P70196 original sequence version: 144 DIP protein P70196 original sequence version: 83 DIP protein P70196 original sequence version: 98 DIP protein P70196 original sequence version: 104 DIP protein P70196 original sequence version: 112 DIP protein P70196 original sequence version: 163 F8389250425E4E2D nr0b2_human Nuclear receptor subfamily 0 group B member 2 NR0B2 SHP Orphan nuclear receptor SHP Small heterodimer partner protein protein human Homo sapiens Human MSTSQPGACPCQGAASRPAILYALLSSSLKAVPRPRSRCLCRQHRPVQLCAPHRTCREALDVLAKTVAFLRNLPSFWQLPPQDQRRLLQGCWGPLFLLGLAQDAVTFEVAEAPVPSILKKILLEEPSSSGGSGQLPDRPQPSLAAVQWLQCCLESFWSLELSPKEYACLKGTILFNPDVPGLQAASHIGHLQQEAHWVLCEVLEPWCPAAQGRLTRVLLTASTLKSIPTSLLGDLFFRPIIGDVDIAGLLGDMLLLR DIP protein Q15466 original sequence version: 79 DIP protein Q15466 original sequence version: 113 14BEE2B3FF46154A tf65_mouse Transcription factor p65 Nuclear factor NF-kappa-B p65 subunit Rela Nfkb3 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 protein protein mouse Mus musculus Mouse MDDLFPLIFPSEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGYYEADLCPDRSIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFHVPIEEQRGDYDLNAVRLCFQVTVRDPAGRPLLLTPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFNGPTEPRPPTRRIAVPTRNSTSVPKPAPQPYTFPASLSTINFDEFSPMLLPSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTPGPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSEFQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDEDFSSIADMDFSALLSQISS DIP protein Q04207 original sequence version: 158 DIP protein Q04207 original sequence version: 117 DIP protein Q04207-1 original sequence version: 117 DIP protein Q04207-1 original sequence version: 118 DIP protein Q04207 original sequence version: 119 DIP protein Q04207 original sequence version: 141 DIP protein Q04207-1 original sequence version: 141 DIP protein Q04207-1 original sequence version: 149 DIP protein Q04207 original sequence version: 149 DIP protein Q04207 original sequence version: 168 DIP protein Q04207 original sequence version: 125 BDF1673D2FE398B9 nr0b2-rela-1 3 8 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association Fig. 4E, 7H, S19 LPS or MSP nr0b2-traf6 3 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association Fig. 5D LPS traf6-nr0b2 3 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association Fig. S7 LPS nr0b2-rela-3 2 5 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 4F, 4G LPS nr0b2-tf65 2 8 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 4F, 4G LPS nr0b2-traf6-1 2 6 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 5E LPS traf6-nr0b2-1 4 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association Fig. 5B, 5C LPS nr0b2-traf6-2 1 6 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 5G nr0b2-rela-4 3 8 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait association association Fig. 5F