MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it The role of trimerization in the osmoregulated betaine transporter BetP. The role of trimerization in the osmoregulated betaine transporter BetP. EMBO Rep. (1469-221X) 2011 imex curation Perez C., Khafizov K., Forrest LR., Krämer R., Ziegler C. Only protein-protein interactions in vitro In vitro bn-page blue native page bn-page weight by comassie molecular weight estimation by coomasie staining weight by comassie Perez C., Khafizov K., Forrest LR., Krämer R., Ziegler C. EMBO Rep. (1469-221X) 2011 Only protein-protein interactions imex curation The role of trimerization in the osmoregulated betaine transporter BetP. The role of trimerization in the osmoregulated betaine transporter BetP. EMBO Rep. (1469-221X) 2011 imex curation Perez C., Khafizov K., Forrest LR., Krämer R., Ziegler C. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Perez C., Khafizov K., Forrest LR., Krämer R., Ziegler C. EMBO Rep. (1469-221X) 2011 Only protein-protein interactions imex curation The role of trimerization in the osmoregulated betaine transporter BetP. The role of trimerization in the osmoregulated betaine transporter BetP. EMBO Rep. (1469-221X) 2011 imex curation Perez C., Khafizov K., Forrest LR., Krämer R., Ziegler C. Only protein-protein interactions in vitro In vitro electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy predetermined predetermined participant predetermined Perez C., Khafizov K., Forrest LR., Krämer R., Ziegler C. EMBO Rep. (1469-221X) 2011 Only protein-protein interactions imex curation The role of trimerization in the osmoregulated betaine transporter BetP. The role of trimerization in the osmoregulated betaine transporter BetP. EMBO Rep. (1469-221X) 2011 imex curation Perez C., Khafizov K., Forrest LR., Krämer R., Ziegler C. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink predetermined predetermined participant predetermined Perez C., Khafizov K., Forrest LR., Krämer R., Ziegler C. EMBO Rep. (1469-221X) 2011 Only protein-protein interactions imex curation betp_corgl Glycine betaine transporter BetP Glycine betaine permease Cgl0892 cg1016 betP protein protein corgl Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) MTTSDPNPKPIVEDAQPEQITATEELAGLLENPTNLEGKLADAEEEIILEGEDTQASLNWSVIVPALVIVLATVVWGIGFKDSFTNFASSALSAVVDNLGWAFILFGTVFVFFIVVIAASKFGTIRLGRIDEAPEFRTVSWISMMFAAGMGIGLMFYGTTEPLTFYRNGVPGHDEHNVGVAMSTTMFHWTLHPWAIYAIVGLAIAYSTFRVGRKQLLSSAFVPLIGEKGAEGWLGKLIDILAIIATVFGTACSLGLGALQIGAGLSAANIIEDPSDWTIVGIVSVLTLAFIFSAISGVGKGIQYLSNANMVLAALLAIFVFVVGPTVSILNLLPGSIGNYLSNFFQMAGRTAMSADGTAGEWLGSWTIFYWAWWISWSPFVGMFLARISRGRSIREFILGVLLVPAGVSTVWFSIFGGTAIVFEQNGESIWGDGAAEEQLFGLLHALPGGQIMGIIAMILLGTFFITSADSASTVMGTMSQHGQLEANKWVTAAWGVATAAIGLTLLLSGGDNALSNLQNVTIVAATPFLFVVIGLMFALVKDLSNDVIYLEYREQQRFNARLARERRVHNEHRKRELAAKRRRERKASGAGKRR 8354B41C834CEFE2 mint 8354B41C834CEFE2 betp 4 BetP 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified P54582:p.Trp101Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W A Tue Jul 12 10:12:03 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 14:46:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp101ala' Tue Jun 30 19:07:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp101Ala' P54582:p.Thr351Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain T A Tue Jul 12 10:09:49 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 16:50:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr351ala' Tue Jun 30 18:39:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr351Ala' Stoichiometry: 3.0 direct interaction direct interaction f4a mint betp-1 1 BetP 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P54582:p.Trp101Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W A Tue Jul 12 10:14:57 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 18:28:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp101ala' Tue Jun 30 17:05:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp101Ala' P54582:p.Thr351Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T A Tue Jul 12 10:14:55 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 18:35:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr351ala' Tue Jun 30 18:55:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr351Ala' P54582:p.Phe345Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain F A Tue Jul 12 10:15:15 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 15:23:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe345ala' Tue Jun 30 16:45:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe345Ala' Stoichiometry: 3.0 direct interaction direct interaction mint f2 sf2 betp-1 1 BetP 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P54582:p.Trp101Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W A Tue Jul 12 10:12:57 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 15:43:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp101ala' Tue Jun 30 19:32:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp101Ala' P54582:p.Thr351Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T A Tue Jul 12 10:12:56 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 15:12:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr351ala' Tue Jun 30 16:01:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr351Ala' Stoichiometry: 3.0 direct interaction direct interaction mint f2 sf2 betp-1 1 BetP 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified P54582:p.Leu330Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Tue Jul 12 10:53:01 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 15:29:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu330ala' Tue Jun 30 19:24:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu330Ala' P54582:p.Leu341Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Tue Jul 12 10:52:59 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 18:50:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu341ala' Tue Jun 30 17:47:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu341Ala' P54582:p.Asn331Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N A Tue Jul 12 10:52:59 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 15:56:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn331ala' Tue Jun 30 18:32:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn331Ala' Stoichiometry: 3.0 direct interaction direct interaction mint f2 sf2 betp-1 1 BetP 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified P54582:p.Trp101Ala mutation disrupting interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W A Tue Jul 12 10:12:38 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 14:44:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp101ala' Tue Jun 30 17:40:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp101Ala' P54582:p.Thr351Ala mutation disrupting interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T A Tue Jul 12 10:52:35 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 16:04:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr351ala' Tue Jun 30 18:27:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr351Ala' Stoichiometry: 3.0 direct interaction direct interaction mint f2 sf2 betp-1 1 BetP 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified P54582:p.Thr351Ala mutation disrupting interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T A Tue Jul 12 10:17:16 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 16:23:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr351ala' Tue Jun 30 19:23:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr351Ala' P54582:p.Trp101Ala mutation disrupting interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W A Tue Jul 12 10:17:22 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 18:04:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp101ala' Tue Jun 30 16:27:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp101Ala' P54582:p.Phe345Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A Tue Jul 12 10:54:24 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 16:19:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe345ala' Tue Jun 30 17:58:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe345Ala' Stoichiometry: 3.0 direct interaction direct interaction mint f3 sf4 betp-1 1 BetP 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified P54582:p.Trp101Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W A Tue Jul 12 10:54:31 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 15:49:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp101ala' Tue Jun 30 18:45:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp101Ala' P54582:p.Thr351Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T A Tue Jul 12 10:54:29 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 18:52:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr351ala' Tue Jun 30 19:04:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr351Ala' Stoichiometry: 3.0 direct interaction direct interaction mint f3 sf4 Wild-type BetP and the mutants BetP-T351A, BetP-W101A/ T351A and BetPDC-W101A/T351A were then reconstituted into E. coli polar lipid liposomes, and their oligomeric state in the membrane was analysed by freeze-fracture electron microscopy (Fig 3; supplementary Fig S4 online)The mean particle diameters and areas are shown in Table 1, and their size distribution is shown in Fig 3B and supplementary Fig S4B online. Wild-type BetP and BetP-T351A are mainly trimeric in the membrane of proteoliposomes, whereas the double and triple mutants are mainly monomeric (approximately 95% monomers and approximately 5% a mixture of trimers and dimers). betp-1 2 BetP 5 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified P54582:p.Trp101Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W A Tue Jul 12 10:15:25 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 14:35:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp101ala' Tue Jun 30 18:20:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp101Ala' P54582:p.Thr351Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T A Tue Jul 12 10:15:28 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 16:46:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr351ala' Tue Jun 30 18:59:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr351Ala' Stoichiometry: 3.0 direct interaction direct interaction sf3 mint betp-1 3 BetP 5 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P54582:p.Trp101Ala mutation disrupting interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W A Tue Jul 12 10:18:39 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 18:53:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp101ala' Tue Jun 30 16:50:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp101Ala' P54582:p.Thr351Ala mutation disrupting interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T A Tue Jul 12 10:18:38 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 16:54:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr351ala' Tue Jun 30 20:02:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr351Ala' Stoichiometry: 3.0 direct interaction direct interaction mint f3 sf4