MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it N-Glycosylation influences the structure and self-association abilities of recombinant nucleolin. N-Glycosylation influences the structure and self-association abilities of recombinant nucleolin. FEBS J. (1742-464X) 2011 imex curation Losfeld ME., Leroy A., Coddeville B., Carpentier M., Mazurier J., Legrand D. Only protein-protein interactions in vitro In vitro bn-page blue native page bn-page weight by comassie molecular weight estimation by coomasie staining weight by comassie Losfeld ME., Leroy A., Coddeville B., Carpentier M., Mazurier J., Legrand D. FEBS J. (1742-464X) 2011 Only protein-protein interactions imex curation N-Glycosylation influences the structure and self-association abilities of recombinant nucleolin. N-Glycosylation influences the structure and self-association abilities of recombinant nucleolin. FEBS J. (1742-464X) 2011 imex curation Losfeld ME., Leroy A., Coddeville B., Carpentier M., Mazurier J., Legrand D. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Losfeld ME., Leroy A., Coddeville B., Carpentier M., Mazurier J., Legrand D. FEBS J. (1742-464X) 2011 Only protein-protein interactions imex curation trfl_human Lactotransferrin LTF LF Talalactoferrin GIG12 Growth-inhibiting protein 12 protein protein human Homo sapiens Human MKLVFLVLLFLGALGLCLAGRRRSVQWCAVSQPEATKCFQWQRNMRKVRGPPVSCIKRDSPIQCIQAIAENRADAVTLDGGFIYEAGLAPYKLRPVAAEVYGTERQPRTHYYAVAVVKKGGSFQLNELQGLKSCHTGLRRTAGWNVPIGTLRPFLNWTGPPEPIEAAVARFFSASCVPGADKGQFPNLCRLCAGTGENKCAFSSQEPYFSYSGAFKCLRDGAGDVAFIRESTVFEDLSDEAERDEYELLCPDNTRKPVDKFKDCHLARVPSHAVVARSVNGKEDAIWNLLRQAQEKFGKDKSPKFQLFGSPSGQKDLLFKDSAIGFSRVPPRIDSGLYLGSGYFTAIQNLRKSEEEVAARRARVVWCAVGEQELRKCNQWSGLSEGSVTCSSASTTEDCIALVLKGEADAMSLDGGYVYTAGKCGLVPVLAENYKSQQSSDPDPNCVDRPVEGYLAVAVVRRSDTSLTWNSVKGKKSCHTAVDRTAGWNIPMGLLFNQTGSCKFDEYFSQSCAPGSDPRSNLCALCIGDEQGENKCVPNSNERYYGYTGAFRCLAENAGDVAFVKDVTVLQNTDGNNNEAWAKDLKLADFALLCLDGKRKPVTEARSCHLAMAPNHAVVSRMDKVERLKQVLLHQQAKFGRNGSDCPDKFCLFQSETKNLLFNDNTECLARLHGKTTYEKYLGPQYVAGITNLKKCSTSPLLEACEFLRK DIP protein P02788 original sequence version: 141 DIP protein P02788 original sequence version: 187 0489CABA6D13C098 nucl_human Nucleolin NCL Protein C23 protein protein human Homo sapiens Human MVKLAKAGKNQGDPKKMAPPPKEVEEDSEDEEMSEDEEDDSSGEEVVIPQKKGKKAAATSAKKVVVSPTKKVAVATPAKKAAVTPGKKAAATPAKKTVTPAKAVTTPGKKGATPGKALVATPGKKGAAIPAKGAKNGKNAKKEDSDEEEDDDSEEDEEDDEDEDEDEDEIEPAAMKAAAAAPASEDEDDEDDEDDEDDDDDEEDDSEEEAMETTPAKGKKAAKVVPVKAKNVAEDEDEEEDDEDEDDDDDEDDEDDDDEDDEEEEEEEEEEPVKEAPGKRKKEMAKQKAAPEAKKQKVEGTEPTTAFNLFVGNLNFNKSAPELKTGISDVFAKNDLAVVDVRIGMTRKFGYVDFESAEDLEKALELTGLKVFGNEIKLEKPKGKDSKKERDARTLLAKNLPYKVTQDELKEVFEDAAEIRLVSKDGKSKGIAYIEFKTEADAEKTFEEKQGTEIDGRSISLYYTGEKGQNQDYRGGKNSTWSGESKTLVLSNLSYSATEETLQEVFEKATFIKVPQNQNGKSKGYAFIEFASFEDAKEALNSCNKREIEGRAIRLELQGPRGSPNARSQPSKTLFVKGLSEDTTEETLKESFDGSVRARIVTDRETGSSKGFGFVDFNSEEDAKAAKEAMEDGEIDGNKVTLDWAKPKGEGGFGGRGGGRGGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE DIP protein P19338 original sequence version: 93 DIP protein P19338 original sequence version: 123 C97F6E34E5CA6727 ncl-ncl 1 R3CT 4 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified P19338:p.Asn492Ala mutation disrupting interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N A Tue Jul 12 10:57:12 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 20:22:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn492ala' Tue Jun 30 18:00:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn492Ala' binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain R3CT 4 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P19338:p.Asn492Ala mutation disrupting interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N A Tue Jul 12 10:20:48 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 17:41:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn492ala' Tue Jun 30 17:20:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn492Ala' direct interaction direct interaction f6 homomint mint As shown in Fig. 6, unlike R3CT and RCT which markedly migrated as single major bands towards the cathode, NCT and R1CT exhibited no or very poor migrations on the gel. Only faint bands can indeed be observed in the upper part of the gel. This suggests that both proteins may be self-associated. ncl-ncl-1 1 RCT 4 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P19338:p.Asn317Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N A Tue Jul 12 10:20:30 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 16:13:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn317ala' Tue Jun 30 17:12:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn317Ala' P19338:p.Asn492Ala mutation disrupting interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N A Tue Jul 12 10:57:07 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 14:56:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn492ala' Tue Jun 30 16:55:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn492Ala' RCT 4 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P19338:p.Asn317Ala mutation disrupting interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N A Tue Jul 12 10:57:05 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 17:19:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn317ala' Wed May 02 17:41:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn317ala' Tue Jun 30 15:39:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn317Ala' P19338:p.Asn492Ala mutation disrupting interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N A Tue Jul 12 10:20:33 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 19:33:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn492ala' Tue Jun 30 20:25:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn492Ala' direct interaction direct interaction f6 homomint mint As shown in Fig. 6, unlike R3CT and RCT which markedly migrated as single major bands towards the cathode, NCT and R1CT exhibited no or very poor migrations on the gel. Only faint bands can indeed be observed in the upper part of the gel. This suggests that both proteins may be self-associated. ncl-ncl-2 2 NCT 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain NCT 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction homomint f7 mint ncl-ncl-3 2 R3CT 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P19338:p.Asn492Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N A Tue Jul 12 10:23:07 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 18:20:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn492ala' Tue Jun 30 16:20:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn492Ala' R3CT 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P19338:p.Asn492Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N A Tue Jul 12 10:58:27 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 18:35:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn492ala' Tue Jun 30 17:39:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn492Ala' direct interaction direct interaction homomint f7 mint ncl-ncl-4 2 RCT 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified P19338:p.Asn492Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N A Tue Jul 12 10:58:14 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 16:30:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn492ala' Tue Jun 30 18:25:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn492Ala' P19338:p.Asn317Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N A Tue Jul 12 10:22:52 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 16:06:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn317ala' Tue Jun 30 17:59:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn317Ala' binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain RCT 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified P19338:p.Asn492Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N A Tue Jul 12 10:22:49 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 14:39:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn492ala' Tue Jun 30 18:37:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn492Ala' P19338:p.Asn317Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N A Tue Jul 12 10:22:50 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 19:34:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn317ala' Tue Jun 30 16:51:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn317Ala' binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction homomint f7 mint ncl-ltf 2 NCT 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain lactoferrin 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified direct interaction direct interaction homomint mint f8 we have investigated the ability of the different proteins to interact with lactoferrin using SPR (Biacore 3000). In this aim, NCT, R1CT, R3CT and RCT were immobilized onto a CM5 sensorchip and concentrations ranging from 50 to 800 nM of human lactoferrin were injected. The results of a representative study among three separate studies are shown in Fig. 8. We can observe that all nucleolin-derived proteins, regardless of their N-glycosylation status, interact with similar binding parameters, with an affinity of about several hundred nM, similar to that of native full-size nucleolin reported by Legrand et al. [13]