IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A hormone-dependent module regulating energy balance. A hormone-dependent module regulating energy balance. Cell (0092-8674) 2011 imex curation Wang B., Moya N., Niessen S., Hoover H., Mihaylova M.M., Shaw R.J., Yates J.R., Fischer W.H., Thomas J.B., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis jthomas@salk.edu, montminy@salk.edu 26-Sep-2011: Contacted by JYOTI. jyoti Only protein-protein interactions mouse-hepatocyte Mouse hepatocyte hepatocyte Liver primary cell A hepatocyte is a cell of the main tissue of the liver. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang B., Moya N., Niessen S., Hoover H., Mihaylova M.M., Shaw R.J., Yates J.R., Fischer W.H., Thomas J.B., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis jthomas@salk.edu, montminy@salk.edu Accepted 2011-JUL-06 by JYOTI 26-Sep-2011: Contacted by JYOTI. Cell (0092-8674) 2011 jyoti Only protein-protein interactions imex curation A hormone-dependent module regulating energy balance. A hormone-dependent module regulating energy balance. Cell (0092-8674) 2011 imex curation Wang B., Moya N., Niessen S., Hoover H., Mihaylova M.M., Shaw R.J., Yates J.R., Fischer W.H., Thomas J.B., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis jthomas@salk.edu, montminy@salk.edu 26-Sep-2011: Contacted by JYOTI. jyoti Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Wang B., Moya N., Niessen S., Hoover H., Mihaylova M.M., Shaw R.J., Yates J.R., Fischer W.H., Thomas J.B., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis jthomas@salk.edu, montminy@salk.edu Accepted 2011-JUL-06 by JYOTI 26-Sep-2011: Contacted by JYOTI. Cell (0092-8674) 2011 jyoti Only protein-protein interactions imex curation A hormone-dependent module regulating energy balance. A hormone-dependent module regulating energy balance. Cell (0092-8674) 2011 imex curation Wang B., Moya N., Niessen S., Hoover H., Mihaylova M.M., Shaw R.J., Yates J.R., Fischer W.H., Thomas J.B., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis jthomas@salk.edu, montminy@salk.edu 26-Sep-2011: Contacted by JYOTI. jyoti Only protein-protein interactions in vitro In vitro deacetylase assay deacetylase assay western blot western blot Immuno blot Immuno blot Wang B., Moya N., Niessen S., Hoover H., Mihaylova M.M., Shaw R.J., Yates J.R., Fischer W.H., Thomas J.B., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis jthomas@salk.edu, montminy@salk.edu Accepted 2011-JUL-06 by JYOTI 26-Sep-2011: Contacted by JYOTI. Cell (0092-8674) 2011 jyoti Only protein-protein interactions imex curation A hormone-dependent module regulating energy balance. A hormone-dependent module regulating energy balance. Cell (0092-8674) 2011 imex curation Wang B., Moya N., Niessen S., Hoover H., Mihaylova M.M., Shaw R.J., Yates J.R., Fischer W.H., Thomas J.B., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis jthomas@salk.edu, montminy@salk.edu 26-Sep-2011: Contacted by JYOTI. jyoti Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang B., Moya N., Niessen S., Hoover H., Mihaylova M.M., Shaw R.J., Yates J.R., Fischer W.H., Thomas J.B., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis jthomas@salk.edu, montminy@salk.edu Accepted 2011-JUL-06 by JYOTI 26-Sep-2011: Contacted by JYOTI. Cell (0092-8674) 2011 jyoti Only protein-protein interactions imex curation A hormone-dependent module regulating energy balance. A hormone-dependent module regulating energy balance. Cell (0092-8674) 2011 imex curation Wang B., Moya N., Niessen S., Hoover H., Mihaylova M.M., Shaw R.J., Yates J.R., Fischer W.H., Thomas J.B., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis jthomas@salk.edu, montminy@salk.edu 26-Sep-2011: Contacted by JYOTI. jyoti Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip fingerprinting peptide massfingerprinting fingerprinting Wang B., Moya N., Niessen S., Hoover H., Mihaylova M.M., Shaw R.J., Yates J.R., Fischer W.H., Thomas J.B., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis jthomas@salk.edu, montminy@salk.edu Accepted 2011-JUL-06 by JYOTI 26-Sep-2011: Contacted by JYOTI. Cell (0092-8674) 2011 jyoti Only protein-protein interactions imex curation A hormone-dependent module regulating energy balance. A hormone-dependent module regulating energy balance. Cell (0092-8674) 2011 imex curation Wang B., Moya N., Niessen S., Hoover H., Mihaylova M.M., Shaw R.J., Yates J.R., Fischer W.H., Thomas J.B., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis jthomas@salk.edu, montminy@salk.edu 26-Sep-2011: Contacted by JYOTI. jyoti Only protein-protein interactions in vitro In vitro acetylase assay acetylase assay western blot western blot Immuno blot Immuno blot Wang B., Moya N., Niessen S., Hoover H., Mihaylova M.M., Shaw R.J., Yates J.R., Fischer W.H., Thomas J.B., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis jthomas@salk.edu, montminy@salk.edu Accepted 2011-JUL-06 by JYOTI 26-Sep-2011: Contacted by JYOTI. Cell (0092-8674) 2011 jyoti Only protein-protein interactions imex curation A hormone-dependent module regulating energy balance. A hormone-dependent module regulating energy balance. Cell (0092-8674) 2011 imex curation Wang B., Moya N., Niessen S., Hoover H., Mihaylova M.M., Shaw R.J., Yates J.R., Fischer W.H., Thomas J.B., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis jthomas@salk.edu, montminy@salk.edu 26-Sep-2011: Contacted by JYOTI. jyoti Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang B., Moya N., Niessen S., Hoover H., Mihaylova M.M., Shaw R.J., Yates J.R., Fischer W.H., Thomas J.B., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis jthomas@salk.edu, montminy@salk.edu Accepted 2011-JUL-06 by JYOTI 26-Sep-2011: Contacted by JYOTI. Cell (0092-8674) 2011 jyoti Only protein-protein interactions imex curation hdac4_mouse Histone deacetylase 4 Hdac4 protein protein mouse Mus musculus Mouse MSSQSHPDGLSGRDQPVELLNPARVNHMPSTVDVATALPLQVAPTAVPMDLRLDHQFSLPLEPALREQQLQQELLALKQKQQIQRQILIAEFQRQHEQLSRQHEAQLHEHIKQQQEMLAMKHQQELLEHQRKLERHRQEQELEKQHREQKLQQLKNKEKGKESAVASTEVKMKLQEFVLNKKKALAHRNLNHCISSDPRYWYGKTQHSSLDQSSPPQSGVSASYNHPVLGMYDAKDDFPLRKTASEPNLKLRSRLKQKVAERRSSPLLRRKDGPVATALKKRPLDVTDSACSSAPGSGPSSPNSSSGNVSTENGIAPTVPSAPAETSLAHRLVTREGSVAPLPLYTSPSLPNITLGLPATGPAAGAAGQQDAERLALPALQQRILFPGTHLTPYLSTSPLERDGAAAHNPLLQHMVLLEQPPTQTPLVTGLGALPLHSQSLVGADRVSPSIHKLRQHRPLGRTQSAPLPQNAQALQHLVIQQQHQQFLEKHKQQFQQQQLHLSKIISKPSEPPRQPESHPEETEEELREHQALLDEPYLDRLPGQKEPSLAGVQVKQEPIESEEEEAEATRETEPGQRPATEQELLFRQQALLLEQQRIHQLRNYQASMEAAGIPVSFGSHRPLSRAQSSPASATFPMSVQEPPTKPRFTTGLVYDTLMLKHQCTCGNTNSHPEHAGRIQSIWSRLQETGLRGKCECIRGRKATLEELQTVHSEAHTLLYGTNPLNRQKLDSSLTSVFVRLPCGGVGVDSDTIWNEVHSSGAARLAVGCVVELVFKVATGELKNGFAVVRPPGHHAEESTPMGFCYFNSVAVAAKLLQQRLNVSKILIVDWDVHHGNGTQQAFYNDPNVLYMSLHRYDDGNFFPGSGAPDEVGTGPGVGFNVNMAFTGGLEPPMGDAEYLAAFRTVVMPIANEFAPDVVLVSSGFDAVEGHPTPLGGYNLSAKCFGYLTKQLMGLAGGRLVLALEGGHDLTAICDASEACVSALLGNELEPLPEKVLHQRPNANAVHSMEKVMDIHSKYWRCLQRLSSTVGHSLIEAQKCEKEEAETVTAMASLSVGVKPAEKRSEEEPMEEEPPL DIP protein Q6NZM9 original sequence version: 71 DIP protein Q6NZM9 original sequence version: 84 A3CCC3CCCBB903A0 foxo1_mouse Forkhead box protein O1 Foxo1 Fkhr Foxo1a Forkhead box protein O1A Forkhead in rhabdomyosarcoma protein protein mouse Mus musculus Mouse MAEAPQVVETDPDFEPLPRQRSCTWPLPRPEFNQSNSTTSSPAPSGGAAANPDAAASLASASAVSTDFMSNLSLLEESEDFARAPGCVAVAAAAAASRGLCGDFQGPEAGCVHPAPPQPPPTGPLSQPPPVPPSAAAAAGPLAGQPRKTSSSRRNAWGNLSYADLITKAIESSAEKRLTLSQIYEWMVKSVPYFKDKGDSNSSAGWKNSIRHNLSLHSKFIRVQNEGTGKSSWWMLNPEGGKSGKSPRRRAASMDNNSKFAKSRGRAAKKKASLQSGQEGPGDSPGSQFSKWPASPGSHSNDDFDNWSTFRPRTSSNASTISGRLSPIMTEQDDLGDGDVHSLVYPPSAAKMASTLPSLSEISNPENMENLLDNLNLLSSPTSLTVSTQSSPGSMMQQTPCYSFAPPNTSLNSPSPNYSKYTYGQSSMSPLPQMPMQTLQDSKSSYGGLNQYNCAPGLLKELLTSDSPPHNDIMSPVDPGVAQPNSRVLGQNVMMGPNSVMPAYGSQASHNKMMNPSSHTHPGHAQQTASVNGRTLPHVVNTMPHTSAMNRLTPVKTPLQVPLSHPMQMSALGSYSSVSSCNGYGRMGVLHQEKLPSDLDGMFIERLDCDMESIIRNDLMDGDTLDFNFDNVLPNQSFPHSVKTTTHSWVSG 3FF58636EA85205F q8i9j6_drome Histone deacetylase HDAC4 HDAC4b CG1770 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSSPDDRIPIHDLPSEAGSDERLLHITPATLTLDFKPHPAVDIDQQIMELKKSQELQKQRLINSFQEQSKQMELEHKLQLEHKYQFAVNSHGAFQELRNESMVTAAAAAVAQEQHRQQLHQQQQQHQQQQQQQQQHQQQQQQQQQQARGRDGMKLKQNCSANASPEVKQILNCFILSRKSQAAASNGTTTTSPYRNRGVVKSSSGESLPAGTVTSAHPYKIPQPPPSLLKYESDFPLRKTASEPNLLKIRLKQSVIERKARIGGPAGARRHERLLQAAQRRQQKNSVLTNCNSTPDSGPNSPPSAAALAVGVVGSRGSPTSAPIQEENEEGSQYQPGQRSSINDLPLFSSPSLPNISLGRPHLPNSAQAHAQVNAQVAAQAQAQAQAQAQAHAMFAALAAAQGGCGQPGYYNPLGMAFVGRQPAPLAMIPATGIAPQQPSPVVRSASATSTSSSQASLVGDVAPPQAHAASTILPSSSSYMQQLGSVAGSGVNLHAAAVAAAAAAAAAAGSLPPTNSHGHGHGSHAHPHPHAHGHGHGHGHGIYAGHQHNVPITDAQVAQVHLHKQGHRPLGRTQSAPLPLGHPMLTGAVQLNVVQTHYENSEAERQAYEHQVVNQKVRQTVLTRSGAAAAAAAAAGVSVVREAQLKEEDDDSAAEVMDLTDKKKPPKTVLTSTIATSTSQNLPEALAAAAAAAAYRAPHNASSNSASATKSGIKLRDQEYLQQQREQLLLLQQEEELAKSLMRPLSRTLSSPLVPLGPHGLSQIPDTGQQPAPIATSSSADHIPPVNLSLPHRQHRQLMSTLYASQLRNHQPSASGSPPHKVTTGLAYDPLMLKHSCICGDNAQHPEHSGRLQSVWARLNETDLVKRCDRLRARKATQEELQTVHTEAHAMLFGSNQCQLSRPKLENTLSASFVRLSCGGLGVDLDTTWNEHHTATAARMAAGCVIDLALKTAKGDLRNGFAVVRPPGHHAEANLAMGFCFFNSIAIAAKLLRQRMPEVRRILIVDWDVHHGNGTQQAFYQSPDILYLSIHRHDDGNFFPGTGGPTECGSGAGLGFNVNISWSGALNPPLGDAEYIAAFRTVVMPIARSFNPDIVLVSSGFDAATGHPAPLGGYHVSPACFGFMTRELLQLANGKVVLALEGGYDLAAICDSAQECVRALLGDPAAPIAKAELERPPCQNAINTLQKTIAIQQTHWPCVRMLEHTVGLSALETLKVEHDESETINAMAGLSMQSMHRTLSRDDSEEPMDQDETK A067E29C4ECCD82A q9vyk0_drome Smr CG12719 CG4013 Dmel\CG4013 E52 SMR SMRTER anon-WO0153538.73 l(1)G0060 l(1)G0361 lincRNA.983 smr Dmel_CG4013 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly 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 18231C54E29F46AE akt1_drome RAC serine/threonine-protein kinase Akt1 CG4006 Akt Protein kinase B protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNYLPFVLQRRSTVVASAPAPGSASRIPESPTTTGSNIINIIYSQSTHPNSSPTSGSAEKFSWQQSWPSRTSAAPTHDSGTMSINTTFDLSSPSVTSGHALTEQTQVVKEGWLMKRGEHIKNWRQRYFVLHSDGRLMGYRSKPADSASTPSDFLLNNFTVRGCQIMTVDRPKPFTFIIRGLQWTTVIERTFAVESELERQQWTEAIRNVSSRLIDVGEVAMTPSEQTDMTDVDMATIAEDELSEQFSVQGTTCNSSGVKKVTLENFEFLKVLGKGTFGKVILCREKATAKLYAIKILKKEVIIQKDEVAHTLTESRVLKSTNHPFLISLKYSFQTNDRLCFVMQYVNGGELFWHLSHERIFTEDRTRFYGAEIISALGYLHSQGIIYRDLKLENLLLDKDGHIKVADFGLCKEDITYGRTTKTFCGTPEYLAPEVLDDNDYGQAVDWWGTGVVMYEMICGRLPFYNRDHDVLFTLILVEEVKFPRNITDEAKNLLAGLLAKDPKKRLGGGKDDVKEIQAHPFFASINWTDLVLKKIPPPFKPQVTSDTDTRYFDKEFTGESVELTPPDPTGPLGSIAEEPLFPQFSYQGDMASTLGTSSHISTSTSLASMQ DIP protein Q8INB9 original sequence version: 105 C139380152580934 foxo_drome Forkhead box protein O foxo Afx Protein FKHR CG3143 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MMDGYAQEWPRLTHTDNGLAMDQLGGDLPLDVGFEPQTRARSNTWPCPRPENFVEPTDELDSTKASNQQLAPGDSQQAIQNANAAKKNSSRRNAWGNLSYADLITHAIGSATDKRLTLSQIYEWMVQNVPYFKDKGDSNSSAGWKNSIRHNLSLHNRFMRVQNEGTGKSSWWMLNPEAKPGKSVRRRAASMETSRYEKRRGRAKKRVEALRQAGVVGLNDATPSPSSSVSEGLDHFPESPLHSGGGFQLSPDFRQRASSNASSCGRLSPIRAQDLEPDWGFPVDYQNTTMTQAHAQALEELTGTMADELTLCNQQQQGFSAASGLPSQPPPPPYQPPQHQQAQQQQQQQSPYALNGPASGYNTLQPQSQCLLHRSLNCSCMHNARDGLSPNSVTTTMSPAYPNSEPSSDSLNTYSNVVLDGPADTAALMVQQQQQQQQQQQLSASLEGQCLEVLNNEAQPIDEFNLENFPVGNLECNVEELLQQEMSYGGLLDINIPLATVNTNLVNSSSGPLSISNISNLSNISSNSGSSLSLNQLQAQLQQQQQQQQAQQQQQAQQQQQQHQQHQQQLLLNNNNNSSSSLELATQTATTNLNARVQYSQPSVVTSPPSWVH B472822ACA169BF9 ebi_drome F-box-like/WD repeat-containing protein ebi ebi CG4063 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSFSSDEVNFLVYRYLQESGFLHSAYVFGIESHISQSNINGALVPPAALLTILQKGLLYTEVEWSVGEDGEVARPIEGLSLIDAVMPEVKPLKPIVKTEPGKPGAVDSSAPAGGNQNNNAKPEIKIEPGTGVAGSAGGNKIAGSTTGTSTPTDQSASEVDSSGNAANNAGGTYAGNNGAGGNQASTGGSNSTSTPAGGDLAAPGASQKKSQNSNEAGSSSSGNAGNANATSTDDAASSTSTNGNSSTSSSVEQPTSGLTPAGGTVSTSNPDAAASGGASTATGSKAPSGAVTIRVGAQGNNVQSGSSNAQSSAPSGTISSSTSGGAGTPAALVPMDIDENIEIPESKARVLRGHESEVFICAWNPSRDLLASGSGDSTARIWDMSDANTNSNQLVLRHCIQKGGAEVPSNKDVTSLDWNCDGSLLATGSYDGYARIWKTDGRLASTLGQHKGPIFALKWNKCGNYILSAGVDKTTIIWDASTGQCTQQFAFHSAPALDVDWQTNQAFASCSTDQRIHVCRLGVNEPIKTFKGHTNEVNAIKWCPQGQLLASCSDDMTLKIWSMNRDRCCHDLQAHSKEIYTIKWSPTGPGTNNPNTNLILASASFDSTVRLWDVERGSCIHTLTKHTEPVYSVAFSPDGKHLASGSFDKCVHIWSTQTGQLVHSYKGTGGIFEVCWNSKGTKVGASASDGSVFVLDLRKF 28C6D8D07BB79FB7 q7kts4_drome Histone deacetylase DHDAC3 DmHDAC3 Dmel\CG2128 HDAC Hdac 3 dHDAC3 dmHDA402 hdac3 Dmel_CG2128 Hdac3 HDAC3 CG2128 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTDRRVSYFYNADVGNFHYGAGHPMKPQRLAVTHSLVMNYGLHKKMKIYRPYKASAQDMLRFHSDEYIAYLQQVTPQNIQCNSVAYTKYLAHFSVGEDCPVFDGLFDFCAMYTGASLEGAQKLNHNHSDICINWSGGLHHAKKFEASGFCYVNDIVIGILELLKYHPRVLYIDIDVHHGDGVQEAFYLTDRVMTASFHKYGNYFFPGTGDMYEIGAESGRYYSVNVPLKEGIDDQSYFQVFKPIISAIMDFYRPTAIVLQCGADSLAGDRLGCFSLSTKGHGECVKFVKELNVPTLVVGGGGYTLRNVARCWTHETSLLVDQDIENDLPATEYYDFFAPDFTLHPEINSRQDNANSKQYLELIVKHVYENLKMCQHSPSVQMVQTPPDVDLEELRSNREEASDPDVRISVADEDKLVDAKNEFYDGDQDQDKPDSAES 7527C32A40C4BA41 q4qqa7_drome Sik3 CG15072 CG18604 Dmel\CG42856 EMK KIAA0999 SIK SIK3 CG42856 Dmel_CG42856 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MATTPTAGPAAAPPTSSTPQNYKVPSTSKISVDKLLRVGYYELEKTIGKGNFAVVKLATNIVTKTKVAIKIIDKTCLNEEYLNKTFREIAILKSLRHPHITRLYEVMESQSMIYLVTEYAPNGEIFDHLVANGRMKEPEAARVFTQLVSAVHYCHRRGVVHRDLKAENVLLDKDMNIKLADFGFSNHYEEGATLKTWCGSPPYAAPEVFQGLEYDGPKSDIWSLGVVLYALVCGALPFDGKTILELKSRVVLGKFRIPFFMSQECEQLIRNMLVVEPDRRYTIKQIIKHRWLSEWQSEMQEQERFGDMSPGSGTVSKSASTSSLGSASDSPPQLDSVVMTHMLQLPGLTADMIAQSVHEQRFDNIYAIYNLLHDKLQQRRRENQRLQHHASLAYSRSRKTSITTGVVDRSEPVKQESLDRLSPLSNANASSSALGFGWSDVAVDLEKYGDFELECLARSNDPPVNAQHLSAHAGGGVNGANTRRHTVGPGDVAHEQALANPNVPPIDFKCPPQCSDPTQPVPYYPVNLPMLQNQPLHNLTIKDQHLLKPPVVMGASSFGRRASDGGANLHIYYPATGTVVGPAQGQQMDTAGYYINPNCGTDPLAVQELSPLNEQSVAQMQCCQENATGECNEELQSYMQKRGGTQRHTVGCTEDLSVAHGSGAGSQSQAPTTSSNMRQRRTGLLTVTERPPGALERLTKIL 441253EA5484647D sik3 2 16 putative self putative self putative self putative self region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined drome Drosophila melanogaster (Fruit fly) Fruit fly phosphorylation phosphorylation reaction phosphorylation true Fig. 2B akt1-q4qqa7 2 12 enzyme enzyme unspecified role unspecified role drome Drosophila melanogaster (Fruit fly) Fruit fly 16 enzyme target enzyme target substrate substrate unspecified role unspecified role Q4QQA7:p.[Tyr281Ala;Ser293Ala;Ser401Ala;Thr486Ala] mutation mutation certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain T A Wed May 02 17:58:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr281ala,ser293ala,ser401ala,thr486ala' Tue Jun 30 15:45:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr281Ala;Ser293Ala;Ser401Ala;Thr486Ala]' Q4QQA7:p.Lys70Met mutation mutation certain certain sequence position certain certain certain sequence position certain K M Wed May 02 20:09:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys70met' Tue Jun 30 16:25:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys70Met' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined drome Drosophila melanogaster (Fruit fly) Fruit fly phosphorylation phosphorylation reaction phosphorylation Fig. 2D SIK3 Thr/Ser/Tyr mutations alter kinetics of interaction. Lsy-70 mutation makes SIK3 catalytically inactive. hdac4-q4qqa7 2 10 enzyme target enzyme target substrate substrate neutral component neutral component Q8I9J6:p.[Ser242Ala;Ser576Ala;Ser751Ala] mutation mutation certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Wed May 02 17:40:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser242ala,ser576ala,ser751ala' Tue Jun 30 16:14:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser242Ala;Ser576Ala;Ser751Ala]' 16 enzyme enzyme neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined drome Drosophila melanogaster (Fruit fly) Fruit fly phosphorylation phosphorylation reaction phosphorylation Fig. 2B Ser mutations in HDAC4 alter kinetics of reaction. hdac4-foxo 4 10 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey physical association physical association foxo-hdac4 3 10 enzyme enzyme neutral component neutral component 13 enzyme target enzyme target substrate substrate neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region ac1res monoacetylated residue Acetyl Acetylation (N terminus, N epsilon of Lysine, O of Serine) (Ac) Acetylation Ac1Res undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined deacetylation deacetylation reaction deacetylation Fig. 6E hdac4-foxo-1 7 10 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey physical association physical association Supp. FIg. 6A Overexpression of constitutively active SIK3T.196E disrupted the FOXO:HDAC interaction ep300-foxo 6 13 enzyme target enzyme target substrate substrate neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined acetylation acetylation reaction acetylation Supp. Fig. 6G hdac4-ebi 5 14 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role prey prey association association Supp. Fig. 6H foxo1-hdac4 1 9 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association Supp. Fig. 7C