IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Role of the C. elegans U2 snRNP protein MOG-2 in sex determination, meiosis, and splice site selection. Role of the C. elegans U2 snRNP protein MOG-2 in sex determination, meiosis, and splice site selection. imex curation 2011-04-06: Puoti A.; Department of Biology, University of Fribourg, Switzerland sequence of U2 RNA not given hence 4D not curated. Also the library used is provided but the clone picked up not mentioned in the text. alessandro.puoti@unifr.ch 21-Dec-2011: Contacted by JYOTI. orchard Only protein-protein interactions caeel Caenorhabditis elegans fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence 2011-04-06: Puoti A.; Department of Biology, University of Fribourg, Switzerland sequence of U2 RNA not given hence 4D not curated. Also the library used is provided but the clone picked up not mentioned in the text. alessandro.puoti@unifr.ch Accepted 2011-MAY-11 by ORCHARD 21-Dec-2011: Contacted by JYOTI. orchard Only protein-protein interactions imex curation Role of the C. elegans U2 snRNP protein MOG-2 in sex determination, meiosis, and splice site selection. Role of the C. elegans U2 snRNP protein MOG-2 in sex determination, meiosis, and splice site selection. imex curation 2011-04-06: Puoti A.; Department of Biology, University of Fribourg, Switzerland sequence of U2 RNA not given hence 4D not curated. Also the library used is provided but the clone picked up not mentioned in the text. alessandro.puoti@unifr.ch 21-Dec-2011: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined 2011-04-06: Puoti A.; Department of Biology, University of Fribourg, Switzerland sequence of U2 RNA not given hence 4D not curated. Also the library used is provided but the clone picked up not mentioned in the text. alessandro.puoti@unifr.ch Accepted 2011-MAY-11 by ORCHARD orchard Only protein-protein interactions imex curation Role of the C. elegans U2 snRNP protein MOG-2 in sex determination, meiosis, and splice site selection. Role of the C. elegans U2 snRNP protein MOG-2 in sex determination, meiosis, and splice site selection. imex curation 2011-04-06: Puoti A.; Department of Biology, University of Fribourg, Switzerland sequence of U2 RNA not given hence 4D not curated. Also the library used is provided but the clone picked up not mentioned in the text. alessandro.puoti@unifr.ch 21-Dec-2011: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro pull down pull down autoradiography autoradiography 2011-04-06: Puoti A.; Department of Biology, University of Fribourg, Switzerland sequence of U2 RNA not given hence 4D not curated. Also the library used is provided but the clone picked up not mentioned in the text. alessandro.puoti@unifr.ch Accepted 2011-MAY-11 by ORCHARD 21-Dec-2011: Contacted by JYOTI. orchard Only protein-protein interactions imex curation q21323_caeel rnp-3 K08D10.3 CELE_K08D10.3 protein protein caeel Caenorhabditis elegans MADINPNHTIYVNNLNEKVKKDELKRSLHMVFTQFGEIIQLMSFRKEKMRGQAHIVFKEVSSASNALRALQGFPFYGKPMRIQYAREDSDVISRAKGTFVEKRQKSTKIAKKPYEKPAKNGKSAAEPTQKEPQETDGPGLPNNILFCSNIPEGTEPEQIQTIFSQFPGLREVRWMPNTKDFAFIEYESEDLSEPARQALDNFRITPTQQITVKFASK u2af2_caeel Splicing factor U2AF 65 kDa subunit uaf-1 Y92C3B.2 U2 auxiliary factor 65 kDa subunit U2 snRNP auxiliary factor large subunit protein protein caeel Caenorhabditis elegans MSDHQDGMKLEDIERQFLDVAQREGGLEAIQPTTGPLENEENLKSSTGGGGGEDDNDRKKRKRSRSRDRDTRRRSRSRDRGERRGGGGGGDRDRSRSRERRRGGGGRDEPRRRGGDDEARSRREPEPQKPREPKKYRFWDVPPTGFETTTPMEYKNMQAAGQVPRGSVQSAVPVVGPSVTCQSRRLYVGNIPFGCNEEAMLDFFNQQMHLCGLAQAPGNPILLCQINLDKNFAFIEFRSIDETTAGMAFDGINFMGQQLKVRRPRDYQPSQNTFDMNSRMPVSTIVVDSANKIFIGGLPNYLTEDQVKELLCSFGPLKAFSLNVDSQGNSKGYAFAEYLDPTLTDQAIAGLNGMQLGDKQLVVQLACANQQRHNTNLPNSASAIAGIDLSQGAGRATEILCLMNMVTEDELKADDEYEEILEDVRDECSKYGIVRSLEIPRPYEDHPVPGVGKVFVEFASTSDCQRAQAALTGRKFANRTVVTSYYDVDKYHNRQF 1A967DE89DC9373C ru2a_caeel Probable U2 small nuclear ribonucleoprotein A' Masculinization of germline protein 2 Spliceosome-associated protein mog-2 mog-2 sap-1 H20J04.8 protein protein caeel Caenorhabditis elegans MVRLTTELFAERPQFVNSVNMREINLRGQKIPVIENMGVTRDQFDVIDLTDNDIRKLDNFPTFSRLNTLYLHNNRINYIAPDIATKLPNLKTLALTNNNICELGDIEPLAECKKLEYVTFIGNPITHKDNYRMYMIYKLPTVRVIDFNRVRLTEREAAKKMFKGKSGKKARDAIQKSVHTEDPSEIEPNENSSGGGARLTDEDREKIKEAIKNAKSLSEVNYLQSILASGKVPEKGWNRQMDQNGADGEAMES ru2a-q21323 2 4 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association 4A q21323-ru2a 2 4 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 4A ru2a-q21323-1 3 4 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 4B ru2a-uaf_1 1 5 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization 4E