MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it The oligomeric state of CtBP determines its role as a transcriptional co-activator and co-repressor of Wingless targets. The oligomeric state of CtBP determines its role as a transcriptional co-activator and co-repressor of Wingless targets. EMBO J. (0261-4189) 2011 imex curation Bhambhani C., Chang JL., Akey DL., Cadigan KM. Only protein-protein interactions drome-kc167 Drosophila melanogaster Kc167 cells kc167 Drosophila anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Bhambhani C., Chang JL., Akey DL., Cadigan KM. EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation ctbp_drome C-terminal-binding protein CtBP CG7583 dCtBP protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDKNLMMPKRSRIDVKGNFANGPLQARPLVALLDGRDCSIEMPILKDVATVAFCDAQSTSEIHEKVLNEAVGALMWHTIILTKEDLEKFKALRIIVRIGSGTDNIDVKAAGELGIAVCNVPGYGVEEVADTTMCLILNLYRRTYWLANMVREGKKFTGPEQVREAAHGCARIRGDTLGLVGLGRIGSAVALRAKAFGFNVIFYDPYLPDGIDKSLGLTRVYTLQDLLFQSDCVSLHCTLNEHNHHLINEFTIKQMRPGAFLVNTARGGLVDDETLALALKQGRIRAAALDVHENEPYNVFQGALKDAPNLICTPHAAFFSDASATELREMAATEIRRAIVGNIPDVLRNCVNKEYFMRTPPAAAAGGVAAAVYPEGALHHRAHSTTPHDGPHSTTNLGSTVGGGPTTVAQAAAAAVAAAAAAALLPSPVPSHLSPQVGGLPLGIVSSQSPLSAPDPNNHLSSSIKTEVKAESTEAP DIP protein O46036 original sequence version: 97 DD5F85535BEB56C1 ctbp-ctbp 1 CtBP 2 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell O46036:p.Asn138Arg mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N R Tue Jul 12 10:54:50 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 20:08:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn138arg' Tue Jun 30 19:59:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn138Arg' ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O46036:p.Arg142Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Tue Jul 12 10:54:52 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 16:04:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg142ala' Tue Jun 30 18:01:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg142Ala' O46036:p.Arg141Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Tue Jul 12 10:16:52 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 19:22:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg141ala' Tue Jun 30 19:43:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg141Ala' O46036:p.Cys134Tyr mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain C Y Tue Jul 12 10:54:57 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 15:17:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys134tyr' Tue Jun 30 18:48:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys134Tyr' CtBP 2 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell O46036:p.Asn138Arg mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N R Tue Jul 12 10:54:50 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 16:18:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn138arg' Tue Jun 30 17:16:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn138Arg' flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O46036:p.Arg142Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Tue Jul 12 10:16:48 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 19:48:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg142ala' Tue Jun 30 19:29:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg142Ala' O46036:p.Arg141Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Tue Jul 12 10:16:49 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 15:06:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg141ala' Tue Jun 30 18:32:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg141Ala' O46036:p.Cys134Tyr mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain C Y Tue Jul 12 10:16:51 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 15:14:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys134tyr' Tue Jun 30 17:59:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys134Tyr' physical association physical association homomint mint As expected, co-expression of CtBPBasic and CtBPAcidic led to association of these molecules at levels similar to those seen with CtBPWT (Supplementary Figure S4; Figure 6B, lane 2). f1b sf4 sf6a Mutations also disrupted the ability of CtBPMono to homo-oligomerize as judged by this assay (Figure 1B). These results demonstrate that CtBPMono cannot dimerize, resulting in a forced mono- mer version of CtBP. ctbp-ctbp-1 1 CtBP 2 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell v5 tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O46036:p.Arg173Glu mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R E Tue Jul 12 10:18:50 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 17:43:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg173glu' Tue Jun 30 19:26:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg173Glu' O46036:p.Arg171Glu mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R E Tue Jul 12 10:18:50 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 16:04:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg171glu' Tue Jun 30 18:20:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg171Glu' CtBP 2 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell O46036:p.Arg171Glu mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R E Tue Jul 12 10:17:03 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 16:22:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg171glu' Tue Jun 30 18:02:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg171Glu' flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O46036:p.Arg173Glu mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R E Tue Jul 12 10:56:06 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 17:01:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg173glu' Tue Jun 30 16:43:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg173Glu' physical association physical association homomint mint As expected, co-expression of CtBPBasic and CtBPAcidic led to association of these molecules at levels similar to those seen with CtBPWT (Supplementary Figure S4; Figure 6B, lane 2). Co-expression of differentially tagged versions of CtBPAcidic did not result in an appreciable co-IP (Figure 6B, lane 1). f6b sf6b f7a f7b ctbp-ctbp-2 1 CtBP 2 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell v5 tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined CtBP 2 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association homomint mint f7a f7b