IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The Mre11:Rad50 structure shows an ATP-dependent molecular clamp in DNA double-strand break repair. The Mre11:Rad50 structure shows an ATP-dependent molecular clamp in DNA double-strand break repair. Cell (0092-8674) 2011 imex curation Lammens K., Bemeleit DJ., Mockel C., Clausing E., Schele A., Hartung S., Schiller CB., Lucas M., Angermuller C., Soding J., Strasser K., Hopfner KP. SPR data not captured as sequence of the nucleic acid probe was not given in the paper. hopfner@lmb.uni-muenchen.de 26-Sep-2011: Contacted by JYOTI. mgt Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink ms participant Identification by mass spectrometry ms participant Lammens K., Bemeleit DJ., Mockel C., Clausing E., Schele A., Hartung S., Schiller CB., Lucas M., Angermuller C., Soding J., Strasser K., Hopfner KP. 1,6-Hexane-bisvinylsulfone SPR data not captured as sequence of the nucleic acid probe was not given in the paper. hopfner@lmb.uni-muenchen.de Accepted 2011-JUL-20 by MGT 26-Sep-2011: Contacted by JYOTI. Cell (0092-8674) 2011 mgt Only protein-protein interactions imex curation The Mre11:Rad50 structure shows an ATP-dependent molecular clamp in DNA double-strand break repair. The Mre11:Rad50 structure shows an ATP-dependent molecular clamp in DNA double-strand break repair. Cell (0092-8674) 2011 imex curation Lammens K., Bemeleit DJ., Mockel C., Clausing E., Schele A., Hartung S., Schiller CB., Lucas M., Angermuller C., Soding J., Strasser K., Hopfner KP. SPR data not captured as sequence of the nucleic acid probe was not given in the paper. hopfner@lmb.uni-muenchen.de 26-Sep-2011: Contacted by JYOTI. mgt Only protein-protein interactions in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Lammens K., Bemeleit DJ., Mockel C., Clausing E., Schele A., Hartung S., Schiller CB., Lucas M., Angermuller C., Soding J., Strasser K., Hopfner KP. 1,6-Hexane-bisvinylsulfone SPR data not captured as sequence of the nucleic acid probe was not given in the paper. hopfner@lmb.uni-muenchen.de Accepted 2011-JUL-20 by MGT 26-Sep-2011: Contacted by JYOTI. Cell (0092-8674) 2011 mgt Only protein-protein interactions imex curation The Mre11:Rad50 structure shows an ATP-dependent molecular clamp in DNA double-strand break repair. The Mre11:Rad50 structure shows an ATP-dependent molecular clamp in DNA double-strand break repair. Cell (0092-8674) 2011 imex curation Lammens K., Bemeleit DJ., Mockel C., Clausing E., Schele A., Hartung S., Schiller CB., Lucas M., Angermuller C., Soding J., Strasser K., Hopfner KP. SPR data not captured as sequence of the nucleic acid probe was not given in the paper. hopfner@lmb.uni-muenchen.de 26-Sep-2011: Contacted by JYOTI. mgt Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column anti tag western anti tag western blot anti tag western Lammens K., Bemeleit DJ., Mockel C., Clausing E., Schele A., Hartung S., Schiller CB., Lucas M., Angermuller C., Soding J., Strasser K., Hopfner KP. 1,6-Hexane-bisvinylsulfone SPR data not captured as sequence of the nucleic acid probe was not given in the paper. hopfner@lmb.uni-muenchen.de Accepted 2011-JUL-20 by MGT 26-Sep-2011: Contacted by JYOTI. Cell (0092-8674) 2011 mgt Only protein-protein interactions imex curation The Mre11:Rad50 structure shows an ATP-dependent molecular clamp in DNA double-strand break repair. The Mre11:Rad50 structure shows an ATP-dependent molecular clamp in DNA double-strand break repair. Cell (0092-8674) 2011 imex curation Lammens K., Bemeleit DJ., Mockel C., Clausing E., Schele A., Hartung S., Schiller CB., Lucas M., Angermuller C., Soding J., Strasser K., Hopfner KP. SPR data not captured as sequence of the nucleic acid probe was not given in the paper. hopfner@lmb.uni-muenchen.de 26-Sep-2011: Contacted by JYOTI. mgt Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Lammens K., Bemeleit DJ., Mockel C., Clausing E., Schele A., Hartung S., Schiller CB., Lucas M., Angermuller C., Soding J., Strasser K., Hopfner KP. BMOE SPR data not captured as sequence of the nucleic acid probe was not given in the paper. hopfner@lmb.uni-muenchen.de Accepted 2011-JUL-20 by MGT 26-Sep-2011: Contacted by JYOTI. Cell (0092-8674) 2011 mgt Only protein-protein interactions imex curation The Mre11:Rad50 structure shows an ATP-dependent molecular clamp in DNA double-strand break repair. The Mre11:Rad50 structure shows an ATP-dependent molecular clamp in DNA double-strand break repair. Cell (0092-8674) 2011 imex curation Lammens K., Bemeleit DJ., Mockel C., Clausing E., Schele A., Hartung S., Schiller CB., Lucas M., Angermuller C., Soding J., Strasser K., Hopfner KP. SPR data not captured as sequence of the nucleic acid probe was not given in the paper. hopfner@lmb.uni-muenchen.de 26-Sep-2011: Contacted by JYOTI. mgt Only protein-protein interactions in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Lammens K., Bemeleit DJ., Mockel C., Clausing E., Schele A., Hartung S., Schiller CB., Lucas M., Angermuller C., Soding J., Strasser K., Hopfner KP. SPR data not captured as sequence of the nucleic acid probe was not given in the paper. hopfner@lmb.uni-muenchen.de Accepted 2011-JUL-20 by MGT 26-Sep-2011: Contacted by JYOTI. Cell (0092-8674) 2011 mgt Only protein-protein interactions imex curation The Mre11:Rad50 structure shows an ATP-dependent molecular clamp in DNA double-strand break repair. The Mre11:Rad50 structure shows an ATP-dependent molecular clamp in DNA double-strand break repair. Cell (0092-8674) 2011 imex curation Lammens K., Bemeleit DJ., Mockel C., Clausing E., Schele A., Hartung S., Schiller CB., Lucas M., Angermuller C., Soding J., Strasser K., Hopfner KP. SPR data not captured as sequence of the nucleic acid probe was not given in the paper. hopfner@lmb.uni-muenchen.de 26-Sep-2011: Contacted by JYOTI. mgt Only protein-protein interactions in vitro In vitro atpase assay atpase assay predetermined predetermined participant predetermined Lammens K., Bemeleit DJ., Mockel C., Clausing E., Schele A., Hartung S., Schiller CB., Lucas M., Angermuller C., Soding J., Strasser K., Hopfner KP. SPR data not captured as sequence of the nucleic acid probe was not given in the paper. hopfner@lmb.uni-muenchen.de Accepted 2011-JUL-20 by MGT 26-Sep-2011: Contacted by JYOTI. Cell (0092-8674) 2011 mgt Only protein-protein interactions imex curation The Mre11:Rad50 structure shows an ATP-dependent molecular clamp in DNA double-strand break repair. The Mre11:Rad50 structure shows an ATP-dependent molecular clamp in DNA double-strand break repair. Cell (0092-8674) 2011 imex curation Lammens K., Bemeleit DJ., Mockel C., Clausing E., Schele A., Hartung S., Schiller CB., Lucas M., Angermuller C., Soding J., Strasser K., Hopfner KP. SPR data not captured as sequence of the nucleic acid probe was not given in the paper. hopfner@lmb.uni-muenchen.de 26-Sep-2011: Contacted by JYOTI. mgt Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lammens K., Bemeleit DJ., Mockel C., Clausing E., Schele A., Hartung S., Schiller CB., Lucas M., Angermuller C., Soding J., Strasser K., Hopfner KP. SPR data not captured as sequence of the nucleic acid probe was not given in the paper. hopfner@lmb.uni-muenchen.de Accepted 2011-JUL-20 by MGT 26-Sep-2011: Contacted by JYOTI. Cell (0092-8674) 2011 mgt Only protein-protein interactions imex curation phix174_dsdna Enterobacteria phage phiX174 linear dsDNA ds dna double stranded deoxyribonucleic acid ds DNA ds dna bpphx Enterobacteria phage phiX174 Bacteriophage phi-X174 amp-pnp AMP-PNP small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) atp ATP Adenosine 5'-triphosphate Adenosine triphosphate ADENOSINE-5'-TRIPHOSPHATE Adephos Adetol Adynol Atipi ATP Atriphos Cardenosine Fosfobion Glucobasin H4atp Myotriphos Triadenyl Triphosphaden adenosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) mre11_pyrfu DNA double-strand break repair protein Mre11 mre11 pfMre11 PF1166 protein protein pyrfu Pyrococcus furiosus MKFAHLADIHLGYEQFHKPQREEEFAEAFKNALEIAVQENVDFILIAGDLFHSSRPSPGTLKKAIALLQIPKEHSIPVFAIEGNHDRTQRGPSVLNLLEDFGLVYVIGMRKEKVENEYLTSERLGNGEYLVKGVYKDLEIHGMKYMSSAWFEANKEILKRLFRPTDNAILMLHQGVREVSEARGEDYFEIGLGDLPEGYLYYALGHIHKRYETSYSGSPVVYPGSLERWDFGDYEVRYEWDGIKFKERYGVNKGFYIVEDFKPRFVEIKVRPFIDVKIKGSEEEIRKAIKRLIPLIPKNAYVRLNIGWRKPFDLTEIKELLNVEYLKIDTWRIKERTDEESGKIGLPSDFFTEFELKIIDILGEKDFDDFDYIIKLITEGKVEEEGPLEEAVKKVSEEKGKTVRQKIESIPKKKRGTLDSWLGGAR DIP protein Q8U1N9 original sequence version: 56 639092C2F9C24DCD q9x1x0_thema Nuclease SbcCD subunit D sbcD TM_1635 protein protein thema Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) MINLKELKILHTSDWHLGVTSWTSSRPVDRREELKKALDKVVEEAEKREVDLILLTGDLLHSRNNPSVVALHDLLDYLKRMMRTAPVVVLPGNHDWKGLKLFGNFVTSISSDITFVMSFEPVDVEAKRGQKVRILPFPYPDESEALRKNEGDFRFFLESRLNKLYEEALKKEDFAIFMGHFTVEGLAGYAGIEQGREIIINRALIPSVVDYAALGHIHSFREIQKQPLTIYPGSLIRIDFGEEADEKGAVFVELKRGEPPRYERIDASPLPLKTLYYKKIDTSALKSIRDFCRNFPGYVRVVYEEDSGILPDLMGEIDNLVKIERKSRREIEEVLRESPEEFKEELDKLDYFELFKEYLKKREENHEKLLKILDELLDEVKKSEA rad50_thema Probable DNA double-strand break repair Rad50 ATPase rad50 TM_1636 protein protein thema Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) MRPERLTVRNFLGLKNVDIEFQSGITVVEGPNGAGKSSLFEAISFALFGNGIRYPNSYDYVNRNAVDGTARLVFQFERGGKRYEIIREINALQRKHNAKLSEILENGKKAAIAAKPTSVKQEVEKILGIEHRTFIRTVFLPQGEIDKLLISPPSEITEIISDVFQSKETLEKLEKLLKEKMKKLENEISSLQALYTAIWKYLEENDLEVLKSELKTVSEKKKELLKKREELQKEEEQLKRLLEKYRELVKKKERLRVLSLRRNELQKEVIYEQKVKKAKELEPLFREIYLRQREFERFSQELNSREKRYKELESEKEAISKEIPVHRERLSKLEEIGEKIKEELDLLEKVLKASRPLLEQRIRLKENLTRLEEEFRRLVGEKEKREKELLSIEKTENETKNELEKLLDELSILKKDHMKWLAYQIASSLNEGDTCPVCGGVFHGKVEAVEFNIDEFEKLDQKRSELENTLNVLKERKKSLSSLIEDLLMKIEEGKKNLKSIRNQIEKIEEELHRLGYSEDLEEKLDEKRKKLRKIEEERHSISQKITAADVQISQIENQLKEIKGEIEAKRETLKEQREEMDQLKSDFFDRLRKIGIGFEEFRILVKEEVKDAEKELGVVETEIRLLEESLKELESENVRDVSEDYEKVRNQLEALSQEISDLERKEGRLNHLIEETLRRERELKSLEKKLKEMSDEYNNLDLLRKYLFDKSNFSRYFTGRVLEAVLKRTKAYLDILTNGRFDIDFDDEKGGFIIKDWGIERPARGLSGGERALISISLAMSLAEVASGRLDAFFIDEGFSSLDTENKEKIASVLKELERLNKVIVFITHDREFSEAFDRKLRITGGVVVNE magnesium(2+) Magnesium(2+) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) adenosine 5'-[gamma-thio]triphosphate Adenosine 5'-[gamma-thio]triphosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) q9x1x0-rad50 7 13 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. Table 1 3.4A Rworking = 25.8% Rfree = 29.5% amp_pnp-magnesium_2_ 7 14 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Supp. Table 2 1.9A Rworking = 15.7% Rfree = 19.1% adenosine_5__-q9x1x0 5 12 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 15 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Fig. 3B atp-q9x1x0 5 13 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 3B rad50-1 1 13 unspecified role unspecified role neutral component neutral component Q9X1X1:p.[Pro31Cys;Glu806Cys] mutation mutation certain certain sequence position certain certain certain sequence position certain P C certain certain sequence position certain certain certain sequence position certain E C Wed May 02 16:06:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro31cys,glu806cys' Tue Jun 30 19:50:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro31Cys;Glu806Cys]' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 3C crosslinking efficiency substantially increases further in the presence of both ATPgammaS and DNA. q9x1x0-rad50-1 3 13 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. Fig. 3A-3D Expt +/- cross-linking agents suggest there is no interactions between Mre11:Rad50 heterotetramers. phix174-q9x1x0-1 2 8 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. Fig. 3E Expt +/- cross-linking agents suggest there is no interactions between Mre11:Rad50 heterotetramers. atp-q9x1x0-2 6 10 enzyme target enzyme target substrate substrate neutral component neutral component 12 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9X1X0:p.Phe291Ser mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F S Wed May 02 15:37:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe291ser' Tue Jun 30 16:32:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe291Ser' ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9X1X1:p.Ile760Cys mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I C Wed May 02 17:31:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile760cys' Tue Jun 30 18:13:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile760Cys' ecoli Escherichia coli (strain K12) atpase reaction atpase reaction Supp. Fig. 3G q9x1x0 4 12 unspecified role unspecified role neutral component neutral component Q9X1X0:p.Ser110Cys mutation mutation certain certain sequence position certain certain certain sequence position certain S C Wed May 02 14:58:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser110cys' Tue Jun 30 17:22:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser110Cys' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 5C mre11-1 4 11 unspecified role unspecified role neutral component neutral component Q8U1N9:p.Phe101Cys mutation mutation certain certain sequence position certain certain certain sequence position certain F C Wed May 02 18:49:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe101cys' Tue Jun 30 16:42:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe101Cys' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 5D