I2D I2D Annexin-1 interacts with NEMO and RIP1 to constitutively activate IKK complex and NF-κB: implication in breast cancer metastasis. Annexin-1 interacts with NEMO and RIP1 to constitutively activate IKK complex and NF-κB: implication in breast cancer metastasis. Oncogene (0950-9232) 2011 imex curation Bist P., Leow S.C., Phua Q.H., Shu S., Zhuang Q., Loh W.T., Nguyen T.H., Zhou J.B., Hooi S.C., Lim L.H. Cancer - Interactions investigated in the context of cancer A Sephacryl 200 column. linalim@nus.edu.sg 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions Curated by: I2D - Interologous Interaction Database human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bist P., Leow S.C., Phua Q.H., Shu S., Zhuang Q., Loh W.T., Nguyen T.H., Zhou J.B., Hooi S.C., Lim L.H. Cancer - Interactions investigated in the context of cancer A Sephacryl 200 column. linalim@nus.edu.sg Accepted 2011-SEP-12 by MGT 23-Sept-2011: Contacted by JYOTI. Oncogene (0950-9232) 2011 mgt Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Annexin-1 interacts with NEMO and RIP1 to constitutively activate IKK complex and NF-κB: implication in breast cancer metastasis. Annexin-1 interacts with NEMO and RIP1 to constitutively activate IKK complex and NF-κB: implication in breast cancer metastasis. Oncogene (0950-9232) 2011 imex curation Bist P., Leow S.C., Phua Q.H., Shu S., Zhuang Q., Loh W.T., Nguyen T.H., Zhou J.B., Hooi S.C., Lim L.H. Cancer - Interactions investigated in the context of cancer A Sephacryl 200 column. linalim@nus.edu.sg 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions Curated by: I2D - Interologous Interaction Database human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Bist P., Leow S.C., Phua Q.H., Shu S., Zhuang Q., Loh W.T., Nguyen T.H., Zhou J.B., Hooi S.C., Lim L.H. Cancer - Interactions investigated in the context of cancer A Sephacryl 200 column. linalim@nus.edu.sg Accepted 2011-SEP-12 by MGT 23-Sept-2011: Contacted by JYOTI. Oncogene (0950-9232) 2011 mgt Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Annexin-1 interacts with NEMO and RIP1 to constitutively activate IKK complex and NF-κB: implication in breast cancer metastasis. Annexin-1 interacts with NEMO and RIP1 to constitutively activate IKK complex and NF-κB: implication in breast cancer metastasis. Oncogene (0950-9232) 2011 imex curation Bist P., Leow S.C., Phua Q.H., Shu S., Zhuang Q., Loh W.T., Nguyen T.H., Zhou J.B., Hooi S.C., Lim L.H. Cancer - Interactions investigated in the context of cancer A Sephacryl 200 column. linalim@nus.edu.sg 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions Curated by: I2D - Interologous Interaction Database human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Bist P., Leow S.C., Phua Q.H., Shu S., Zhuang Q., Loh W.T., Nguyen T.H., Zhou J.B., Hooi S.C., Lim L.H. Cancer - Interactions investigated in the context of cancer A Sephacryl 200 column. Author confirms 1% formaldehyde was used for ChIP experiments. linalim@nus.edu.sg Accepted 2011-SEP-12 by MGT 23-Sept-2011: Contacted by JYOTI. Oncogene (0950-9232) 2011 mgt Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Annexin-1 interacts with NEMO and RIP1 to constitutively activate IKK complex and NF-κB: implication in breast cancer metastasis. Annexin-1 interacts with NEMO and RIP1 to constitutively activate IKK complex and NF-κB: implication in breast cancer metastasis. Oncogene (0950-9232) 2011 imex curation Bist P., Leow S.C., Phua Q.H., Shu S., Zhuang Q., Loh W.T., Nguyen T.H., Zhou J.B., Hooi S.C., Lim L.H. Cancer - Interactions investigated in the context of cancer A Sephacryl 200 column. linalim@nus.edu.sg 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions Curated by: I2D - Interologous Interaction Database human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Bist P., Leow S.C., Phua Q.H., Shu S., Zhuang Q., Loh W.T., Nguyen T.H., Zhou J.B., Hooi S.C., Lim L.H. Cancer - Interactions investigated in the context of cancer A Sephacryl 200 column. linalim@nus.edu.sg Accepted 2011-SEP-12 by MGT 23-Sept-2011: Contacted by JYOTI. Oncogene (0950-9232) 2011 mgt Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Annexin-1 interacts with NEMO and RIP1 to constitutively activate IKK complex and NF-κB: implication in breast cancer metastasis. Annexin-1 interacts with NEMO and RIP1 to constitutively activate IKK complex and NF-κB: implication in breast cancer metastasis. Oncogene (0950-9232) 2011 imex curation Bist P., Leow S.C., Phua Q.H., Shu S., Zhuang Q., Loh W.T., Nguyen T.H., Zhou J.B., Hooi S.C., Lim L.H. Cancer - Interactions investigated in the context of cancer A Sephacryl 200 column. linalim@nus.edu.sg 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions Curated by: I2D - Interologous Interaction Database human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bist P., Leow S.C., Phua Q.H., Shu S., Zhuang Q., Loh W.T., Nguyen T.H., Zhou J.B., Hooi S.C., Lim L.H. Cancer - Interactions investigated in the context of cancer A Sephacryl 200 column. linalim@nus.edu.sg Accepted 2011-SEP-12 by MGT 23-Sept-2011: Contacted by JYOTI. Oncogene (0950-9232) 2011 mgt Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Annexin-1 interacts with NEMO and RIP1 to constitutively activate IKK complex and NF-κB: implication in breast cancer metastasis. Annexin-1 interacts with NEMO and RIP1 to constitutively activate IKK complex and NF-κB: implication in breast cancer metastasis. Oncogene (0950-9232) 2011 imex curation Bist P., Leow S.C., Phua Q.H., Shu S., Zhuang Q., Loh W.T., Nguyen T.H., Zhou J.B., Hooi S.C., Lim L.H. Cancer - Interactions investigated in the context of cancer A Sephacryl 200 column. linalim@nus.edu.sg 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions Curated by: I2D - Interologous Interaction Database human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma confocal microscopy confocal microscopy immunostaining immunostaining Bist P., Leow S.C., Phua Q.H., Shu S., Zhuang Q., Loh W.T., Nguyen T.H., Zhou J.B., Hooi S.C., Lim L.H. Cancer - Interactions investigated in the context of cancer A Sephacryl 200 column. linalim@nus.edu.sg Accepted 2011-SEP-12 by MGT 23-Sept-2011: Contacted by JYOTI. Oncogene (0950-9232) 2011 mgt Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database nemo_human NF-kappa-B essential modulator IKBKG FIP3 NEMO NF-kappa-B essential modifier Inhibitor of nuclear factor kappa-B kinase subunit gamma IkB kinase-associated protein 1 FIP-3 protein protein human Homo sapiens Human MNRHLWKSQLCEMVQPSGGPAADQDVLGEESPLGKPAMLHLPSEQGAPETLQRCLEENQELRDAIRQSNQILRERCEELLHFQASQREEKEFLMCKFQEARKLVERLGLEKLDLKRQKEQALREVEHLKRCQQQMAEDKASVKAQVTSLLGELQESQSRLEAATKECQALEGRARAASEQARQLESEREALQQQHSVQVDQLRMQGQSVEAALRMERQAASEEKRKLAQLQVAYHQLFQEYDNHIKSSVVGSERKRGMQLEDLKQQLQQAEEALVAKQEVIDKLKEEAEQHKIVMETVPVLKAQADIYKADFQAERQAREKLAEKKELLQEQLEQLQREYSKLKASCQESARIEDMRKRHVEVSQAPLPPAPAYLSSPLALPSQRRSPPEEPPDFCCPKCQYQAPDMDTLQIHVMECIE DIP protein Q9Y6K9 original sequence version: 85 DIP protein Q9Y6K9 original sequence version: 118 DIP protein Q9Y6K9 original sequence version: 123 DIP protein Q9Y6K9 original sequence version: 141 DIP protein Q9Y6K9 original sequence version: 125 DIP protein Q9Y6K9 original sequence version: 156 DIP protein Q9Y6K9 original sequence version: 83 DIP protein Q9Y6K9 original sequence version: 132 DIP protein Q9Y6K9 original sequence version: 124 DIP protein Q9Y6K9 original sequence version: 90 322D1037881447FF anxa1_human Annexin A1 ANXA1 ANX1 LPC1 Annexin-1 Annexin I Lipocortin I Calpactin II Chromobindin-9 p35 Phospholipase A2 inhibitory protein Calpactin-2 protein protein human Homo sapiens Human MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN DIP protein P04083 original sequence version: 146 14B42E1FA4178EC0 tf65_human Transcription factor p65 Nuclear factor NF-kappa-B p65 subunit RELA NFKB3 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 protein protein human Homo sapiens Human MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS DIP protein Q04206 original sequence version: 170 DIP protein Q04206 original sequence version: 173 DIP protein Q04206 original sequence version: 99 DIP protein Q04206-1 original sequence version: 99 DIP protein Q04206 original sequence version: 168 DIP protein Q04206 original sequence version: 128 DIP protein Q04206 original sequence version: 131 DIP protein Q04206 original sequence version: 153 DIP protein Q04206 original sequence version: 185 DIP protein Q04206 original sequence version: 181 DIP protein Q04206 original sequence version: 183 8147A6AF9F2445C6 cxcr4_human_gene Human CXCR4 gene gene gene human Homo sapiens Human ripk1_human Receptor-interacting serine/threonine-protein kinase 1 RIPK1 RIP Cell death protein RIP RIP1 Receptor-interacting protein 1 protein protein human Homo sapiens Human MQPDMSLNVIKMKSSDFLESAELDSGGFGKVSLCFHRTQGLMIMKTVYKGPNCIEHNEALLEEAKMMNRLRHSRVVKLLGVIIEEGKYSLVMEYMEKGNLMHVLKAEMSTPLSVKGRIILEIIEGMCYLHGKGVIHKDLKPENILVDNDFHIKIADLGLASFKMWSKLNNEEHNELREVDGTAKKNGGTLYYMAPEHLNDVNAKPTEKSDVYSFAVVLWAIFANKEPYENAICEQQLIMCIKSGNRPDVDDITEYCPREIISLMKLCWEANPEARPTFPGIEEKFRPFYLSQLEESVEEDVKSLKKEYSNENAVVKRMQSLQLDCVAVPSSRSNSATEQPGSLHSSQGLGMGPVEESWFAPSLEHPQEENEPSLQSKLQDEANYHLYGSRMDRQTKQQPRQNVAYNREEERRRRVSHDPFAQQRPYENFQNTEGKGTAYSSAASHGNAVHQPSGLTSQPQVLYQNNGLYSSHGFGTRPLDPGTAGPRVWYRPIPSHMPSLHNIPVPETNYLGNTPTMPFSSLPPTDESIKYTIYNSTGIQIGAYNYMEIGGTSSSLLDSTNTNFKEEPAAKYQAIFDNTTSLTDKHLDPIRENLGKHWKNCARKLGFTQSQIDEIDHDYERDGLKEKVYQMLQKWVMREGIKGATVGKLAQALHQCSRIDLLSSLIYVSQN DIP protein Q13546 original sequence version: 158 DIP protein Q13546-1 original sequence version: 140 DIP protein Q13546 original sequence version: 146 DIP protein Q13546-1 original sequence version: 146 DIP protein Q13546 original sequence version: 134 DIP protein Q13546 original sequence version: 109 976E2428D525A9B2 anxa1-ikbkg 2 8 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component physical association physical association 2b,c In controls, ANXA1 is observed only in high molecular weight complexes. Overexpression of ANXA1 results in both high and low molecular weight complexes for ANXA1, NEMO and RIP suggesting that ANXA1 overexpression encourages oligomerization of NEMO and RIP. anxa1-ikbkg-1 4 8 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component physical association physical association 2d anxa1-nemo 1 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association 2e Immunoprecipitations were carried out on gel-filtration elution fractions of MCF7 cells transfected with ANXA1. A Sephacryl 200 column was used. NEMO and ANXA1 are coimmunoprecipitated in fractions 5-9. ripk1-anxa1 1 8 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association 3a nemo-anxa1 1 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association 3a nemo-anxa1-1 5 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association 3b ripk1-anxa1-1 5 8 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association 3b anxa1-rela 1 8 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association 3d ripk1-ikbkg 5 7 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association 3c ikbkg-ripk1-4 5 7 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association 3c anxa1-ikbkg-3 6 7 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization 3e anxa1-ripk1 6 11 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization 3e rela-cxcr4 3 9 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey association association 6f SDF1 alpha (stromal-derived factor 1 alpha or CXCL12) recruits NF-kappaB to the CXCR4 promoter in MCF7 cells. Notably, NF-kappaB was recruited to CXCR4 promoter in MCF7-V5 cells without SDF1alpha treatment.