IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Cdc42 GTPase-activating protein (CdGAP) interacts with the SH3D domain of Intersectin through a novel basic-rich motif. Cdc42 GTPase-activating protein (CdGAP) interacts with the SH3D domain of Intersectin through a novel basic-rich motif. FEBS Lett. (0014-5793) 2011 imex curation Primeau M., Ben Djoudi Ouadda A., Lamarche-Vane N. nathalie.lamarche@mcgill.ca 23-Sept-2011: Contacted by JYOTI. orchard Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. pull down pull down anti tag western anti tag western blot anti tag western Primeau M., Ben Djoudi Ouadda A., Lamarche-Vane N. nathalie.lamarche@mcgill.ca Accepted 2011-SEP-12 by ORCHARD 23-Sept-2011: Contacted by JYOTI. FEBS Lett. (0014-5793) 2011 orchard Only protein-protein interactions imex curation Cdc42 GTPase-activating protein (CdGAP) interacts with the SH3D domain of Intersectin through a novel basic-rich motif. Cdc42 GTPase-activating protein (CdGAP) interacts with the SH3D domain of Intersectin through a novel basic-rich motif. FEBS Lett. (0014-5793) 2011 imex curation Primeau M., Ben Djoudi Ouadda A., Lamarche-Vane N. nathalie.lamarche@mcgill.ca 23-Sept-2011: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay antibody detection identification by antibody antibody detection Primeau M., Ben Djoudi Ouadda A., Lamarche-Vane N. nathalie.lamarche@mcgill.ca Accepted 2011-SEP-12 by ORCHARD 23-Sept-2011: Contacted by JYOTI. FEBS Lett. (0014-5793) 2011 orchard Only protein-protein interactions imex curation Cdc42 GTPase-activating protein (CdGAP) interacts with the SH3D domain of Intersectin through a novel basic-rich motif. Cdc42 GTPase-activating protein (CdGAP) interacts with the SH3D domain of Intersectin through a novel basic-rich motif. FEBS Lett. (0014-5793) 2011 imex curation Primeau M., Ben Djoudi Ouadda A., Lamarche-Vane N. nathalie.lamarche@mcgill.ca 23-Sept-2011: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro pull down pull down autoradiography autoradiography Primeau M., Ben Djoudi Ouadda A., Lamarche-Vane N. nathalie.lamarche@mcgill.ca Accepted 2011-SEP-12 by ORCHARD 23-Sept-2011: Contacted by JYOTI. FEBS Lett. (0014-5793) 2011 orchard Only protein-protein interactions imex curation rhg31_mouse Rho GTPase-activating protein 31 Arhgap31 Cdgap Kiaa1204 Cdc42 GTPase-activating protein protein protein mouse Mus musculus Mouse MKNKGAKQKLKRKGAASAFGCDLTEYLESSGQDVPYVLKSCAEFIETHGIVDGIYRLSGITSNIQRLRQEFGSDQCPDLTREVYLQDIHCVGSLCKLYFRELPNPLLTYELYEKFTEAVSHRPEEGQLARIQNVILELPPPHYRTLEYLIRHLAHIASFSSKTNMHARNLALVWAPNLLRSKKIEATICNGDAAFLAVRVQQVVIEFILNHADQIFNGGAPGALQQDESRTITKSLTLPALSLPMKLVSLEEAQARSLATNHPARKERRENSLPEIVPPPFHTVLELPDNKRKLSSKSKKWKSIFNLGRSGSDSKSKLSRNGSVFVRGQRLSVEKATIRPAKSMDSLCSVPVEGKENKGNFSRTVTTGGFFIPATKMHASSTGSSCDLSKEGEWGQEGMPAGAEGGCEVGGQIRPLPEQLKVFRPIGDPESEQSAPKLLGMFYTSSDSPGKSVFTSSLFQMEPSPRHQRKALNISEPFAVSVPLRVSAVISTNSTPCRTPPKELQSLSSLEEFSFQGSESGGWPEEEKPLGAESFPGSVTKKAATEDTKPEPEVPGRAECSQSPPLDPGTQVEKKTLHVSLGSQVSKEAEKRPKAEKVMEESQGASQPKPSTPQESLGAGTEPLILHEMDEEDLAQALIWPEIQQELKIIESEEEFSSLPPAAQKTSPIPESSPAPFPFPEAPGSLPSSSAPREVWTRDAANQSIQEAAILTDREKLEPVCSLLESESQQELSPDPASLAPLEMLLFEKVSSPARIEIGGPRNLSPPLTPAPPPPTPLEEEPEVLLSKEGPDREDAARDSRTDVYTEQPTPKESPGIPTPCQREEAIASPNEKQNARHAVPENKGPGLPSPTKEVDIIPQEEGGAPHSAQEPSDCDEDDTVTDPAQHGLEMVEPWEEPQWVTSPLHSPTLKEVQESQTQGSQGHRLERRLCHRPSLRQSHSLDSKTTGNSHWTLEAPFSSSCANLETERNYEPLQPPAARTKIAGLEEKALKAFREFSGLKGLEVLPSQKGPSGIQPKPVETNFMGLAEGKEQEPQLELSNRQMKHSDVPGPDSSKESSPRAQDSTLPGEHPLQLQLKNTECGPSKGKHRPSSLNLDSATPIADLFRLENGAPFSSPGIELSELGDTKVTWMSSSHCKAAPWNSQDTQDLDIVAHTLTGRRNSAPVSVSAVRTSFMVKMCQAKAVPVIPPKIQYTQIPQPLPSQSTGEGGAQPLERSQEEPGSTPEIPQKSTKDDSPSSLGSPEEEQPKQETGASASRRQASITSCMYEGSSCSPEPSASTLASTQDAVVQCRKRTSETEPSGDNLLSSKLERASGGPKAFHRSRPGRPQSLILFPIMDHLPSSPTVIDSKVLLSPIRSPTQTVSPGLLCGELAENTWITPEGVTLRNKMTIPKNGQRLETSTSCFYQPQRRSVILDGRSGRQIE 00A39A0664D1A558 itsn1_mouse Intersectin-1 EH and SH3 domains protein 1 Itsn1 Ese1 Itsn protein protein mouse Mus musculus Mouse MAQFPTPFGGSLDVWAITVEERAKHDQQFLSLKPIAGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSTLPPVMKQQPVAISSAPAFGIGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPPAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASAPPAAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGMSVISSSSVDQRLPEEPSSEDEQQPEKKLPVTFEDKKRENFERGSVELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEGTVVLKARRKTLEFELEALNDKKHQLEGKLQDIRCRLATQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILSDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLREQLDEVERETRSKLQEIDVFNNQLKELREIHSKQQLQKQRSLEAARLKQKEQERKSLELEKQKEDAQRRVQERDKQWLEHVQQEEQPRPRKPHEEDRLKREDSVRKKEAEERAKPEMQDKQSRLFHPHQEPAKLATQAPWSTTEKGPLTISAQESVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPTPAKPVTDLTSAPAPKLALRETPAPLPVTSSEPSTTPNNWADFSSTWPSSSNEKPETDNWDTWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKSDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPVRKSTSIDTGPTESPASLKRVASPAAKPAIPGEEFIAMYTYESSEQGDLTFQQGDVIVVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYAATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTELPKTAVQPAVCQVIGMYDYTAQNDDELAFSKGQIINVLNKEDPDWWKGEVSGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLTESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP BCF5038160E8208E rhg30_mouse Rho GTPase-activating protein 30 Arhgap30 Rho-type GTPase-activating protein 30 protein protein mouse Mus musculus Mouse MKSRQKGKKKGSSKERVFGCDLREHLQHSGQEVPQVLRSCAEFVQEYGVVDGIYRLSGVSSNIQKLRQEFETERKPDLRRDVYLQDIHCVSSLCKAYFRELPDPLLTYRLYDKFAEAVAVQLEPERLVKILEVLQELPIQNYRTLEFLMRHLVHMASFSAQTNMHARNLAIVWAPNLLRSKDIEASGFNGTAAFMEVRVQSIVVEFILTHVDQLFRGDSLSAGVDLESGWKSLPGARASGSSEDLMPTSLPYHLPSILQAGDGPPQIRPYHTIIEIAEHKRKGSLKVRKWRSIFNLGRSGHETKRKLPLRVEDREEKSSKGTLRPAKSMDSLSAAAGASDEPEGLVGSSSSQPSSLMPESLESNSMEGEQEPEAEAPGSANSEPGTPRAGRSAVRALGSSRAERCAGVHISDPYNVNLPLHITSILSVPPNIISNVSLVRLTRGLECPALQPRPSPALGPGPPGSVGPLASDEKSEARSVPGPLDDSSPAAMTPALEDSLSQEVQDSFSFLEDLSSSEPEWVGVEEREVAKAEAAGAAGAAAFSLGEDDPGMGYLEELLRVGPQVEEFSVEPPLDDLSLDDTQYVLAPNCCSLDSAVSTPDVEEDYGEEVFLSAYDDLSPLLGPKPINWEGVGSLEEEAAGCGKQPPTQDEEEQACSETRQEKEAKPRSTSDNREEAEATPETEMEAGKADAEGGEAERSQKVMDSFKEGSREELEAKEENSEGREVESIKETKDVEKIIGEPGKDEEREIGREEGAEKGDDTPVDSDMDPEHVFQEDLVLEESWEVVHKHEAEKGRESETKELRRKSDLKSREDQGHSEDSGSPEEGDDRKEGVFSKEQKSIDVETEVMRGVGDHLEEGALSEGPGVELLRVDSTEEINEQTSEMKQAPLQPSEPEGMEAEGQLNPETCDLYSCPCGSAGGVGMRLASTLVQVRQVRSVPVVPPKPQFAKMPSAMCSKIHVAPASPCPRPGRLDGTPGEKAWGSRASWRNGGSLSFDAAVALARERQRTESQGVLRTQTCTGGGDYSLSSRTPPCSMILAHSSRPLSCLERPPEGTEGSEPRSRLSLPPRELHPVVPLVAPQRQTYAFETQTNHGKDEGV F91D1F2B5B67EB9E itsn1-arhgap31 3 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). 4 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 10 13 physical association physical association Figure 1A itsn1-arhgap31-1 1 5 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain A6X8Z5:p.[Lys291Ala;Arg292Ala;Lys293Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain K A Wed May 02 14:53:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys291ala,arg292ala,lys293ala' Tue Jun 30 17:44:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys291Ala;Arg292Ala;Lys293Ala]' A6X8Z5:p.[Lys297Ala;Lys299Ala;Lys300Ala] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain K A Wed May 02 14:53:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys297ala,lys299ala,lys300ala' Tue Jun 30 19:31:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys297Ala;Lys299Ala;Lys300Ala]' A6X8Z5:p.[Lys302Ala;Ser303Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain S A Wed May 02 17:52:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys302ala,ser303ala' Tue Jun 30 18:56:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys302Ala;Ser303Ala]' A6X8Z5:p.[Ile304Ala;Phe305Ala;Leu307Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain L A Wed May 02 15:01:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile304ala,phe305ala,leu307ala' Tue Jun 30 18:58:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile304Ala;Phe305Ala;Leu307Ala]' A6X8Z5:p.[Asp313Ala;Ser314Ala;Lys315Ala;Ser316Ala;Lys317Ala;Leu318Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain L A Wed May 02 16:26:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp313ala,ser314ala,lys315ala,ser316ala,lys317ala,leu318ala' Tue Jun 30 19:30:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp313Ala;Ser314Ala;Lys315Ala;Ser316Ala;Lys317Ala;Leu318Ala]' region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined A6X8Z5:p.Trp301Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W A Wed May 02 16:14:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp301ala' Tue Jun 30 17:23:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp301Ala' A6X8Z5:p.Arg309Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Wed May 02 19:08:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg309ala' Tue Jun 30 16:52:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg309Ala' 19 16 physical association physical association Figure 1B itsn1-arhgap30 1 6 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 34 30 physical association physical association Figure 3B arhgap31-itsn1 2 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 2