IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Skin stem cells orchestrate directional migration by regulating microtubule-ACF7 connections through GSK3β. Skin stem cells orchestrate directional migration by regulating microtubule-ACF7 connections through GSK3β. Cell (0092-8674) 2011 imex curation Wu X., Shen QT., Oristian DS., Lu CP., Zheng Q., Wang HW., Fuchs E. fuchslb@rockefeller.edu mgt Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Wu X., Shen QT., Oristian DS., Lu CP., Zheng Q., Wang HW., Fuchs E. Details of enzyme construct taken from manufacturer's website. fuchslb@rockefeller.edu Accepted 2011-MAR-24 by MGT Cell (0092-8674) 2011 mgt Only protein-protein interactions imex curation Skin stem cells orchestrate directional migration by regulating microtubule-ACF7 connections through GSK3β. Skin stem cells orchestrate directional migration by regulating microtubule-ACF7 connections through GSK3β. Cell (0092-8674) 2011 imex curation Wu X., Shen QT., Oristian DS., Lu CP., Zheng Q., Wang HW., Fuchs E. fuchslb@rockefeller.edu mgt Only protein-protein interactions mouse-som_stem_cell Mouse somatic stem cell somatic_stem_cell Somatic (adult) stem cell anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wu X., Shen QT., Oristian DS., Lu CP., Zheng Q., Wang HW., Fuchs E. fuchslb@rockefeller.edu Accepted 2011-MAR-24 by MGT Cell (0092-8674) 2011 mgt Only protein-protein interactions imex curation macf1_mouse Microtubule-actin cross-linking factor 1 Actin cross-linking family 7 Macf1 Acf7 Aclp7 Macf protein protein mouse Mus musculus Mouse MSSSDEETLSERSCRSERSCRSERSYRSERSGSLSPCPPGDTLPWNLPLHEQKKRKSQDSVLDPAERAVVRVADERDRVQKKTFTKWVNKHLMKVRKHINDLYEDLRDGHNLISLLEVLSGIKLPREKGRMRFHRLQNVQIALDFLKQRQVKLVNIRNDDITDGNPKLTLGLIWTIILHFQISDIYISGESGDMSAKEKLLLWTQKVTAGYTGVKCTNFSSCWSDGKMFNALIHRYRPDLVDMERVQVQSNRENLEQAFEVAERLGVTRLLDAEDVDVPSPDEKSVITYVSSIYDAFPKVPEGGEGISATEVDSRWQEYQSRVDSLIPWIRQHTILMSDKSFPQNPVELKALYNQYIHFKETEILAKEREKGRIKELYKLLEVWIEFGRIKLPQGYHPNHVEEEWGKLIVEMLEREKSLRPAVERLELLLQIANKIQNGALNCEEKLTLAKNTLQADAAHLESGQPVQCESDVIMYIQECEGLIRQLQVDLQILRDEKYYQLEELAFRVMRLQDELVTLRLECTNLYRKGHFSSLELVPPSTLTTTHLKAEPLNKTTHSSSTSWFRKPMTRTELVSISSSEDEGNLRFVYELLSWVEEMQMKLERAEWGNDLPSVELQLETQQHIHTSVEELGSSVKEARLYEGKMSQNFHTSYVETLGKLETQYCKLKETSSFRMRHLQSLHKFVSRATAELIWLNGKEEEELACDWSDSNPNISAKKTYFSELTMELEGKQDVFRSLQDTAEVLSLENHPAKQTVEAYSAAVQSQLQWMKQLCLCVEQHVKENAAYFQFFSDARDLESFLRNLQDSIKRKYTCDRSTSLSRLEDLLQDSMDEKEQLIQSKSSVASLVGRSKTIVQLKPRNPDHVLKSTLSVKAICDYRQIEITICKNDECVLEDNSQRTKWKVISPTGNEAMVPSVCFLIPPPNKEAIEMASRVEQSYQKVMALWHQLHINTKSLISWNYLRKDLDTVQTWSLEKLRSLAPGECHQVMKNLQAHYEDFLQDSHDSALFSVADRLRIEEEVEACKAHFQHLMKSLENEDKEETLAKVYISELKNIRLLLEECEQRLLKQIQSPASSKTDRDARQDITLRIAEQEHTQEDLQHLRSDLDAISMKCNVFLQQSPSGSSATTLRSELNLMVEKMDHVYGLSTVYLNKLKTIDVIVRSMQDAELLVKGYEIKLSQEEAVPADLSALESHRTTLQHWLSDVKDKNSVFSVLDEEITKAKKVAEQLRHPASEPNLDLERYQEKGSQLQERWHRVIAQLETRQSEVESIQEVLRDYRACHGTLIKWIEETTAQQEMMKPGQAEDSRVLSEQLSQQTELFAEIERNQTKLDQCQKFSQQYSTIVKDYELQLMTYKAFVESQQKSPGKRRRMISSSDAITQEFMDLRTRYTALVTLTTQHVKYISDALRRLEEEEKVVEEEKQEHVEKVKDLLGWVSTLARNTQGTTTSSHTSASADIEKAILEQQVLAEELTTKKEQVSEAIKTSQIFLAKHGHKLSEGEKEQISEQLRVLNKTYHDLCDGSANQLQQLQSELAQQTEQKGCRAVAGVIDLGTVEIFPIFRAMQKGLIDQDTGLVLLESQIIMSGLIDPENSEKLSLEEGLTRNFINLPIYQQLLGLRDSLSLVSRLTGTLGSLSVVEAIEKKIISERLGLKVLEVHLATGGFSLPPSENCINLEEAFHQGFIASSLHSELQSHLRSSKNLIDPNTAEKVGLLDLMQRCIIHQESGLKLLPVKQLAGGMVSLKSGRKVSIFRAVQEGLIDRQVTVRLLEAQLFAGGIVDPRTGHRLTVEEAVRHNLIDQDMACAILIRQLQTGGIIDTVTGDRMTIDEAVTNNLVAAKIALVILESLWSFMGLLLPESGEILPITDALEQGIVSTELAHKILHNRQQIEALFLPTLTEIWSWEKATESGILDKDLVNNLRSVCIPDMMPHIQLADSAEQSKVGFAAGKPPVSGPREEGSSHGEKLLFQLMTHSYIHAHTGQRLLLLDQELVEMLTSRDDCQVILPEVFEIQHQRLNTSEALQELYTGTISQISSAKHPRKPCESQFLSQNKDYPSQENCTEAKGERSVVGIECSPAESPERELFLKEQEAIIENVGSLKVINKVKLKLQRPLLGSRKEEQAETLREENISGDPLLVECPEESEGKDLSTEKSKCQTPTKCSFTCHKEQVKTIKDIPSETGTSLIKSQNQMSQFQVDTSVGLRSEFKSEHDMNVNSLEKELKEELLVKDGHKQSQEGQSVADGQTVALEKTDTEDNADEPALLHSSPFEDATLSTLSAQLQDGGIFNEETGQKLLLNEAIAQGLVSSHTAVKLMGKLNMFRGFFDSQTCESLTTEEVIDEGLMDEKLLYNVLMSDKAISGILDPRTHSLCSVKEAVAAGLLDKETATRILEGQVITGGIVDLKRGKKLSVTLASNLGLVDTADQTELINLEKATKGRGAEKAVKERLIELQMETAGLMDPESKAPLTVLQSIDRGILEREAAVYLLTKQVLDGGIIHHISGLRLSVDNAFKHGLIGEDMARQLRKVENFIHYQFFHPQTKEALSFSEAIKLDLVSPDLKREIQEIQDFSGNLGDFIYGQKLTLAKTNKEESLANKTELPSGVMHGVIDPENCTIIPYSELVKKCRIDTESGWRYLEVIPFSDIKDEAGNNVLTPPEAIQLGKVDFASALKVLEAQANTGGIIDMATGKRVTLASALEKKLLDENMARIIASHQMLSGGIIDIYSDQRVTLNDAVEKRLISPELAAMIQVDPLAEQGGTGVCELKGDFLRKELLSESSKTPRESYSKEKHEAVLQAGSLCAPEKAGIRGSNGEKAEKGRKISVEMEGQRQDEKASSDSKVSASILSPFGFEGESSYQVSVTHPCSESCDLKPREETRSCMKKCAVVERDKVVTQIKMVSHVKQSTSGLDAEEARERQGRMVSKEQGSHYETAGNLLSERSVRVDRRVRREMGGEQSVQMSREAAVLSEEESDQEVTIGDEPDSFVKSQSMKMIGNDKGKEAGIEKDISVVCKIEGFPSQMTSKDASLTNQDALPFYTEGETKTVNLCSILKPGEKLSQETASTVQKEPLSSEIPRPERLNSQESDEEPQISDVPHISKGDMAAQITTRQETTDVQDLYITSKSSETKDKIFPSKNYIEKLHQEIPMDPTRSHKLKEATISTLETEGISYLDSSDIKSLCEDSKADHKSCGHQKSKVTTTQAKKSLEVVDLLVRDTEEGSSEDRVGQRGPRVLASLLPEKLPTRTVQSENIRQHDAVIPAISEIREEMALSLPCSVVKVDGKIPKEKHKEILGDEQGPFMAIPSGKGIEGVNPEPCRATQNVFTRRLCLEHDEKLVSYLSLLRDIEMRTKQIQPLELNVAELQDLLGQAKELDRELKDLSTVVSQELECVDRIVISQPQEVPAQLLKALEKDAKNLQKSLDSVSDSWSSRFLHLQSAVEVKKATVLNRHKELQGKLQDLRAWVGRASLTLNSKGCDTETDADSLSHTLQPYKDMKQSMAERKSQLDALALDIQLFISEHPQDLSLQQNQEMLQFLSELQRSFQGLVEHTAAQKDVVQGHLQQVQQEVQVKTLQKQQDTCHKKLEDLCNWVGQAERALERHQGGSSSGKSSLLCAEPKVDLKDLQGDIQSHSTSFATAVKDIEGFLEENQTKLSPQELTALREKLHQAKEQYEVLQERTRVAQKELEEAVTSALQQETEKSKAATELAENKRKIDALLDWVGLLWGHLRESHRLAFQEWSSSLELAMEKQTLAATDGHVDVNQVPETLDRQYELMKARHQELLSQQQNFIVATQSVQSFLDQHSHNLTPEERQKLQEKLGELKEQYAASLARSEAELKQTQALRDELQKFLQDHKEFENWLQQSENELDSMHKGGSSPEALNSLLKRQGSFSEDVISHKGDLRFVTISGQKVLETENNFEEGQEPSATRNLVNEKLKDATERYTTLHSKCIRLGSHLSMLLGQYQQFQSSADSLQAWVLTCEASVGKLLSDTVASDPGVLQQQLATTKQLQEELAEHQVPVEKLQKAAHDLLDIEGEPALDCRPIQETTDSISSRFQNLSCSLDERSALLQKAIAQSQSVQESMESLLQSIREVEQNLERDQVASLSSGVIQEALANNMKLKQDIARQKSSLEATHDMVTRFMETADSNSASVLQGKLAELSQRFQQLQLQQQEKESNLKKLLPQAEMFEQLSNKLQQFMENKSRLLASGNQPDQDIAHFSQQIQELTLAMEDQKENLDTLEHLVTTLGSCGFALDLSQHQDKIQNLKKDFTELQKTVQEREKDASTCQEQLDEFRKLIRTFQKWLKETEGNVPPAKTFVSAKELEKQIEHLKDLISDWESKGALLGEINAKGTALESLIMDITAPDSQAKTGSILPPVGSSVGSVNGYHTCKDLTEIQCDMFDVNSKYEKLWEVLRERQESLQTVFSRMEEVQKEASSVLQWLESKEEVLKAMDATLSPTKTETVKAQAESNKAFLAELEQNSPKIQKVKEALAGLLKTYPNSQEAENWKKMQEDLNSRWEKATEVTVARQKQLEESASHLACFQAAESQLRPWLMEKELMMGVLGPLSIDPNMLKQQVQFMLKEFEARRQQHEQLNEAAQGILTGPGDMSPSASQVHKDLQSISQKWVELTDKLNSRSSQIDQAIVKSTQYQDLLQDLSEKVKAIGQRLSGQSAISTQPEAVKQQLEETSEIRSDLGQLDNEIKEAQTLCQELSLLIGEQYLKDELKKRLETVALPLQGLEDLAADRMNRLQAALASTQQFQQMFDELRTWLDEKQSQQAKNCPISAKLERLQCQLQENEEFQKNLNQHSGSYEVIVAEGEALLLSVPPGEEKKTLQNQLVELRSHWEDLSKKTANRQSRLKDCMQKAQKYQGHVEDLVPWIDECKSKMSELQVTLDPVQLESSLLRSKAMLNEAEKRRSLLEILNSAADILINSSEIDEDEIRDEKAGLNQNMDAITEELQAKTSSLEEMTQRLKEFQESFKNIEKKVEGAKHQLEIFDALGSQACSNKNLEKLKAQQEVLQALEPQVDYLRNFTQGLVEDAPDGSDASPLVHQAEVAQQEFLEVKQRVSSSCLTMENKLEGIGQFHCRVREMFSQLADLDDELDGMGAIGRDTDSLQSQIEDVRLFLNKIQALRFDIEDSEAECRKMLEEEGTLDLLGLKRELEALNKQCGKLTERGKVRQEQLELTLGRVEDFYRKLKALNDAATAAEEGEALQWIVGTEVDVINQQLADFKLFQKDQVDPLQVKLQQVNGLGQGLIQSAGKNCDVQGLEHDMDEINTRWNTLNKKVAQRIAQLQEALLHCGKFQDALEPLLSWLTDTEELIANQKPPSAEYKVVKAQIQEQKLLQRLLDDRKATVDMLQAEGGRIAQSAELADREKITGQLESLERRWTDLLSKAAARQKQLEDILVLAKQFHETAEPISDFLSVTANKLANSEPVGTQTAKIHQQIIRHKALNEEIINRKKNVDQAIKNGQALLKQTTGEEVLLIQEKLDGIKTRYADITLTSSKALRTLEQARQLATKFHSTYEELTGWLREAEEELAASGGQSPTGEQIPQFQQRQKELKKEVMEHRLVLDTVNEVSHALLELVPWRAREGLDKLVSDANEQYKLISDTVGQRVDEIDAAIQRSQQYEQAADAELAWVAETKRKLMALGPIRLEQDQTTAQLQVQKAFSIDIIRHKDSMDELFSHRGEIFSTCGEEQKAVLQEKTECLIQQYEAVSLLNSERYARLERAQVLVNQFWETYEELSPWAEETLALIAQLPPPAVDHEQLRQQQEEMRQLRESIAEHKPHIDKILKIGPQLKELNPEEGKMVEEKYQKAENMYAQIKDEVRQRALALDEAVSQSAQFHDKIEPMLETLENLSSRLRMPPLIPAEVDKIRECISDNKSATVELEKLQPSFEALKRRGEELIGRSQGADKDLAAKEIQDKLDQMVFFWEDIKARSEEREIKFLDVLELAEKFWYDMAALLTTIKDTQDIVHDLESPGIDPSIIKQQVEAAETIKEETDGLHEELEFIRILGADLIFACGETEKPEVKKSIDEMNNAWENLNKTWKERLEKLEDAMQAAVQYQDTLQAMFDWLDNTVIKLCTMPPVGTDLNTVKDQLNEMKEFKVEVYQQQIEMEKLNHQGELMLKKATDETDRDIIREPLTELKHLWENLGEKIAHRQHKLEGALLALGQFQHALEELMSWLTHTEELLDAQRPISGDPKVIEVELAKHHVLKNDVLAHQATVATVNKAGSELLESSAGDDASSLRSRLETMNQCWESVLQKTEEREQQLQSTLQQAQGFHSEIEDFLLELNRMENQLSASKPTGGLPETAREQLDTHMELHSQLRAKEEIYNQLLDKGRLMLLSRGDSGSGSKTEQSVALLEQKWHAVSSKVEERKSKLEEALSLATEFQNSLQEFINWLTLAEQSLNIASPPSLILNTVLSQIEEHKVFANEVNDHRDQIIELDQTGNQLKFLSQKQDVVLIKNLLVSVQSRWEKVVQRSIERGRSLDDARKRAKQFHEAWKKLIDWLEDAESHLDSELEISNDPDKIKLQLSKHKEFQKTLGGKQPVYDTTIRTGRALKEKTLLAGDTQKLDNLLGEVRDKWDTVCGKSVERQHKLEEALLFSGQFMDALQALVDWLYKVEPQLAEDQPVHGDLDLVMNLMDAHKVFQKELGKRTGTVQVLKRSGRELIEGSRDDTTWVKGQLQELSTRWDTVCKLSVSKQSRLEQALKQAEEFRDTVHMLLEWLSEAEQTLRFRGALPDDTEALQSLIDTHKEFMKKVEEKRVDVNTAVAMGEAILAVCHPDCITTIKHWITIIRARFEEVLTWAKQHQQRLETALSELVANAELLEELLAWIQWAETTLIQRDQEPIPQNIDRVKALITEHQSFMEEMTRKQPDVDRVTKTYKRKSVEPTHAPFMEKSRSGSRKSLNQPTPPPMPILSQSEAKNPRINQLSARWQQVWLLALERQRKLNDALDRLEELCPELKEFANFDFDVWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILASKFPTTKLEMTAVADIFDRDGDGYIDYYEFVAALHPNKDAYRPTTDADKIEDEVTRQVAQCKCAKRFQVEQIGENKYRFFLGNQFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRARGRTNIELREKFILPEGASQGMTPFRSRGRRSKPSSRAASPTRSSSSASQSNHSCTSMPSSPATPASGTKVISSSGSKLKRPTPAFHSSRTSLAGDTSNSSSPASTGAKANRADPKKSASRPGSRAGSRAGSRASSRRGSDASDFDLLETQSACSDTSESSAAGGQGSSRRGLTKPSKIPTMSKKTTTASPRTPGPKR 2B68195989DE15C7 g1t8e2_rabit Glycogen synthase kinase-3 beta GSK3B protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MSGRPRTTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKDSSGTGRFTSGVRVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASTPTNATAASDGLFNEQFFALNSKGQISSLI Similar to P49841 1F32A12644F1DB3E gsk3b_mouse Glycogen synthase kinase-3 beta Gsk3b Serine/threonine-protein kinase GSK3B protein protein mouse Mus musculus Mouse MSGRPRTTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASPPANATAASDTNAGDRGQTNNAASASASNST DIP protein Q9WV60 original sequence version: 159 200C3FD1B38B4883 macf1-gsk3b 2 5 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association Immunoblot analyses revealed GSK3β in anti-ACF7 immunoprecipitates of wild-type (WT) but not ACF7 null cell lysates (Figure 2A). Fig. 2A gsk3b-macf1-1 1 3 enzyme target enzyme target substrate substrate neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain 4 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Immunoblot analyses revealed GSK3β in anti-ACF7 immunoprecipitates of wild-type (WT) but not ACF7 null cell lysates (Figure 2A). Fig. 2B Phosphorylation of Macf1 Exact coordinates of MACF1 C-terminal domain not given and has been inferred from the information in the text.