MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Silintaphin-1--interaction with silicatein during structure-guiding bio-silica formation. Silintaphin-1--interaction with silicatein during structure-guiding bio-silica formation. FEBS J. (1742-464X) 2011 Schlossmacher U., Wiens M., Schröder HC., Wang X., Jochum KP., Müller WE. in vitro In vitro electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy predetermined predetermined participant predetermined Schlossmacher U., Wiens M., Schröder HC., Wang X., Jochum KP., Müller WE. FEBS J. (1742-464X) 2011 b6gva0_subdo siltap protein protein sponge Suberites domuncula MSSEETPVEQPTAEETPASTEETPAETPATTETTEPAADDQPAVTEVTEVTETPAEPTPEATTEEPKAEEKKEEVKQKPKKEPKPAPDYRTTKSGWVQKQGLLFKKWQRQFMTLDEEDSVLRWWKSEQRTGSDGALYMKFCVGVSDPKAETTTTWPDDTADRCFVVCSPYRAFFLVAESQEDKNDWIQKLSAAKDKHNTEGRKLITTALISVEGIKASFDNGAADLDKTVKKEGIVEEKKTEELLQLDKTVKKEGIVEEKKTEEAAPTESETAPAEAEGAAAVTIEVEVETPAEEPPKEETPAEEPPKEETPAEVPPKEETPAEEPPKEETPAEEPPKEETPAEEPPKEETPAEDPPKEEETPAEEPPKDEAPAAAEGGEETKAED mint A1A89DE1BB3A9CAB q2mev3_subdo silicaa-g protein protein sponge Suberites domuncula MLVTVVVLGLLGFASAAQPKFEFVEEWQLWKSTHSKMYESQLMELERHLTWLSNKKYIEQHNVNSHIFGFTLAMNQFGDLSELEYADYLGQYRIEDKKSGNYSKTFQRDPLQDYPEAVDWRTKGAVTAVKDQGDCGASYAFSAMGALEGANALAKGNAVSLSEQNIIDCSIPYGNHGCHGGNMYDAFLYVIANEGVDQDSAYPFVGKQSSCNYNSKYKGTSMSGMVSIKSGSESDLQAAVSNVGPVSVAIDGANSAFRFYYSGVYDSSRCSSSSLNHAMVVTGYGSYNGKKYWLAKNSWGTNWGNSGYVMMARNKYNQCGIATDASYPTL mint AAD4B2DB310A17E8 silicaa-g 1 silicatein-α 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 direct interaction direct interaction mint self-assembly of recombinant silicatein-α was investigated in Tris buffer (lacking glycerol) by SEM analysis (Fig. 4). If silicatein-α is added to the buffer at a concentration of 50 μg/ml [2 μM] no structures could be resolved by SEM after 1 min (Fig. 4A). However, after 15 min randomly organized aggregates became visible (Fig. 4B), which increased in size and compactness of packing after 30 min (Fig. 4C). With prolonged time, the aggregates grew further (60 min [Fig. 4D]; 120 min [Fig. 4E]). f4a f4b f4c f4d f4e f4f silicaa_g-siltap 1 silintaphin-1 2 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 1.4 silicatein-α 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 4.1 direct interaction direct interaction mint co-incubation of recombinant silicatein-α and silintaphin-1 in a stoichiometric ratio of 4 to 1 elicited a drastically different self-assembly (Fig. 4G to K) with fractal-like patterns f4g f4h f4i f4j f4k f4l f4m