UniProt UniProt http://www.uniprot.org Molecular Basis of Wnt Activation via the DIX Domain Protein Ccd1. Molecular Basis of Wnt Activation via the DIX Domain Protein Ccd1. J. Biol. Chem. (0021-9258) 2011 mimix curation Liu YT., Dan QJ., Wang J., Feng Y., Chen L., Liang J., Li Q., Lin SC., Wang ZX., Wu JW. jiaweiwu@mail.tsinghua.edu.cn 26-Jun-2011: Contacted by JYOTI. orchard in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Liu YT., Dan QJ., Wang J., Feng Y., Chen L., Liang J., Li Q., Lin SC., Wang ZX., Wu JW. jiaweiwu@mail.tsinghua.edu.cn Accepted 2011-MAY-23 by ORCHARD 26-Jun-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2011 orchard mimix curation Molecular Basis of Wnt Activation via the DIX Domain Protein Ccd1. Molecular Basis of Wnt Activation via the DIX Domain Protein Ccd1. J. Biol. Chem. (0021-9258) 2011 mimix curation Liu YT., Dan QJ., Wang J., Feng Y., Chen L., Liang J., Li Q., Lin SC., Wang ZX., Wu JW. jiaweiwu@mail.tsinghua.edu.cn 26-Jun-2011: Contacted by JYOTI. orchard in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Liu YT., Dan QJ., Wang J., Feng Y., Chen L., Liang J., Li Q., Lin SC., Wang ZX., Wu JW. jiaweiwu@mail.tsinghua.edu.cn Accepted 2011-MAY-23 by ORCHARD 26-Jun-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2011 orchard mimix curation Molecular Basis of Wnt Activation via the DIX Domain Protein Ccd1. Molecular Basis of Wnt Activation via the DIX Domain Protein Ccd1. J. Biol. Chem. (0021-9258) 2011 mimix curation Liu YT., Dan QJ., Wang J., Feng Y., Chen L., Liang J., Li Q., Lin SC., Wang ZX., Wu JW. jiaweiwu@mail.tsinghua.edu.cn 26-Jun-2011: Contacted by JYOTI. orchard in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Liu YT., Dan QJ., Wang J., Feng Y., Chen L., Liang J., Li Q., Lin SC., Wang ZX., Wu JW. jiaweiwu@mail.tsinghua.edu.cn Accepted 2011-MAY-23 by ORCHARD 26-Jun-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2011 orchard mimix curation Molecular Basis of Wnt Activation via the DIX Domain Protein Ccd1. Molecular Basis of Wnt Activation via the DIX Domain Protein Ccd1. J. Biol. Chem. (0021-9258) 2011 mimix curation Liu YT., Dan QJ., Wang J., Feng Y., Chen L., Liang J., Li Q., Lin SC., Wang ZX., Wu JW. jiaweiwu@mail.tsinghua.edu.cn 26-Jun-2011: Contacted by JYOTI. orchard in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Liu YT., Dan QJ., Wang J., Feng Y., Chen L., Liang J., Li Q., Lin SC., Wang ZX., Wu JW. jiaweiwu@mail.tsinghua.edu.cn Accepted 2011-MAY-23 by ORCHARD 26-Jun-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2011 orchard mimix curation Molecular Basis of Wnt Activation via the DIX Domain Protein Ccd1. Molecular Basis of Wnt Activation via the DIX Domain Protein Ccd1. J. Biol. Chem. (0021-9258) 2011 mimix curation Liu YT., Dan QJ., Wang J., Feng Y., Chen L., Liang J., Li Q., Lin SC., Wang ZX., Wu JW. jiaweiwu@mail.tsinghua.edu.cn 26-Jun-2011: Contacted by JYOTI. orchard in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Liu YT., Dan QJ., Wang J., Feng Y., Chen L., Liang J., Li Q., Lin SC., Wang ZX., Wu JW. jiaweiwu@mail.tsinghua.edu.cn Accepted 2011-MAY-23 by ORCHARD 26-Jun-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2011 orchard mimix curation dixc1_human Dixin DIXDC1 CCD1 KIAA1735 DIX domain-containing protein 1 Coiled-coil protein DIX1 protein protein human Homo sapiens Human MLACLTRGNLLDVLQEGFNEQQLQAYVAWVNAQLKKRPAVKPVQDLRQDLRDGVILAYLIEIVAGEKLSGVQLSPGNQQEMKNNVEKVLQFVASKKIRMHQTSAKDIVDGNLKSIMRLVLALAAHFKPGSSRTVNQGRDSRAPLQSHRPHCATAVAQGAAAALADVCHDMSRSGRDVFRYRQRNSSMDEEIENPYWSVRALVQQYEGQQRSPSESSCSSLTSPSPIHSAKSESIITQSEEKADFVIIPAEGIENRTEGTDSPLSRDWRPGSPGTYLETSWEEQLLEQQEYLEKEMEEAKKMISGLQALLLNGSLPEDEQERPLALCEPGVNPEEQLIIIQSRLDQSMEENQDLKKELLKCKQEARNLQGIKDALQQRLTQQDTSVLQLKQELLRANMDKDELHNQNVDLQRKLDERNRLLGEYKKELGQKDRLLQQHQAKLEEALRKLSDVSYHQVDLERELEHKDVLLAHCMKREADEATNYNSHNSQSNGFLLPTAGKGATSVSNRGTSDLQLVRDALRSLRNSFSGHDPQHHTIDSLEQGISSLMERLHVMETQKKQERKVRVKSPRTQVGSEYRESWPPNSKLPHSQSSPTVSSTCTKVLYFTDRSLTPFMVNIPKRLEEVTLKDFKAAIDREGNHRYHFKALDPEFGTVKEEIFHDDDAIPGWEGKIVAWVEEDHGEN E643D1A69B00238C dvl2_mouse Segment polarity protein dishevelled homolog DVL-2 Dvl2 DSH homolog 2 protein protein mouse Mus musculus Mouse MAGSSAGGGGVGETKVIYHLDEEETPYLVKIPVPAERITLGDFKSVLQRPAGAKYFFKSMDQDFGVVKEEISDDNARLPCFNGRVVSWLVSSDTPQPEVAPPAHESRTELVPPPPPLPPLPPERTSGIGDSRPPSFHPNVSSSHENLEPETETESVVSLRRDRPRRRDSSEHGAGGHRPGGPSRLERHLAGYESSSTLMTSELESTSLGDSDEDDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRMERTSSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDMNFENMSNDDAVRVLRDIVHKPGPIVLTVAKCWDPSPQAYFTLPRNEPIQPIDPAAWVSHSAALTGAFPAYPGSSSMSTITSGSSLPDGCEGRGLSVHMDMASVTKAMAAPESGLEVRDRMWLKITIPNAFLGSDVVDWLYHHVEGFPERREARKYASGLLKAGLIRHTVNKITFSEQCYYVFGDLSGGCESYLVNLSLNDNDGSSGASDQDTLAPLPGATPWPLLPTFSYQYPAPHPYSPQPPPYHELSSYTYGGGSASSQHSEGSRSSGSTRSDGGAGRTGRPEERAPESKSGSGSESELSSRGGSLRRGGEPGGTGDGGPPPSRGSTGAPPNLRALPGLHPYGAPSGMALPYNPMMVVMMPPPPPPVSTAVQPPGAPPVRDLGSVPPELTASRQSFHMAMGNPSEFFVDVM DIP protein Q60838 original sequence version: 132 DIP protein Q60838 original sequence version: 63 DIP protein Q60838 original sequence version: 125 DIP protein Q60838 original sequence version: 69 DIP protein Q60838 original sequence version: 91 928A527143BBBBB0 dvl1_mouse Segment polarity protein dishevelled homolog DVL-1 Dvl1 Dvl DSH homolog 1 protein protein mouse Mus musculus Mouse MAETKIIYHMDEEETPYLVKLPVAPERVTLADFKNVLSNRPVHAYKFFFKSMDQDFGVVKEEIFDDNAKLPCFNGRVVSWLVLAEGAHSDAGSQGTDSHTDLPPPLERTGGIGDSRPPSFHPNVASSRDGMDNETGTESMVSHRRERARRRNRDEAARTNGHPRGDRRRDLGLPPDSASTVLSSELESSSFIDSDEEDNTSRLSSSTEQSTSSRLVRKHKCRRRKQRLRQTDRASSFSSITDSTMSLNIITVTLNMERHHFLGISIVGQSNDRGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDVNFENMSNDDAVRVLREIVSQTGPISLTVAKCWDPTPRSYFTIPRADPVRPIDPAAWLSHTAALTGALPRYGTSPCSSAITRTSSSSLTSSVPGAPQLEEAPLTVKSDMSAIVRVMQLPDSGLEIRDRMWLKITIANAVIGADVVDWLYTHVEGFKERREARKYASSMLKHGFLRHTVNKITFSEQCYYVFGDLCSNLASLNLNSGSSGASDQDTLAPLPHPSVPWPLGQGYPYQYPGPPPCFPPAYQDPGFSCGSGSAGSQQSEGSKSSGSTRSSHRTPGREERRATGAGGSGSESDHTVPSGSGSTGWWERPVSQLSRGSSPRSQASAVAPGLPPLHPLTKAYAVVGGPPGGPPVRELAAVPPELTGSRQSFQKAMGNPCEFFVDIM DIP protein P51141 original sequence version: 147 FA21ACAC48FF71E0 dvl2-dvl2-2 2 7 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction fig 2B, fig 3, fig 4, fig 7A dvl2-dixdc1 5 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association fig 6 dixdc1-dvl2 2 6 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction fig 7a dixdc1-dvl2-1 4 7 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction supplementary fig S4 dvl1-dvl1 1 8 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction 2.87 A R value 0.232 R free 0.265 Self-associating head to tail assembly of monomers gives rise to protein filaments. dvl2-dvl2-3 3 7 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction supplemental fig 2 forms oligomers dixdc1-dvl2-2 3 6 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction fig 7b