UniProt UniProt http://www.uniprot.org A nutrient-sensitive interaction between Sirt1 and HNF-1α regulates Crp expression. A nutrient-sensitive interaction between Sirt1 and HNF-1α regulates Crp expression. Aging Cell (1474-9718) 2011 mimix curation Grimm A.A., Brace C.S., Wang T., Stormo G.D., Imai S. Apoptosis - Interactions involving proteins with a function related to apoptosis Species origin of Sirt1 and HNF1alpha constructs confirmed by the authors (e-mail) imaishin@wustl.edu 14-Mar-2012: Contacted by JYOTI. human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Grimm A.A., Brace C.S., Wang T., Stormo G.D., Imai S. Apoptosis - Interactions involving proteins with a function related to apoptosis Species origin of Sirt1 and HNF1alpha constructs confirmed by the authors (e-mail) imaishin@wustl.edu Accepted 2011-DEC-08 by JYOTI 14-Mar-2012: Contacted by JYOTI. Aging Cell (1474-9718) 2011 mimix curation A nutrient-sensitive interaction between Sirt1 and HNF-1α regulates Crp expression. A nutrient-sensitive interaction between Sirt1 and HNF-1α regulates Crp expression. Aging Cell (1474-9718) 2011 mimix curation Grimm A.A., Brace C.S., Wang T., Stormo G.D., Imai S. Apoptosis - Interactions involving proteins with a function related to apoptosis Species origin of Sirt1 and HNF1alpha constructs confirmed by the authors (e-mail) imaishin@wustl.edu 14-Mar-2012: Contacted by JYOTI. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Grimm A.A., Brace C.S., Wang T., Stormo G.D., Imai S. Apoptosis - Interactions involving proteins with a function related to apoptosis Species origin of Sirt1 and HNF1alpha constructs confirmed by the authors (e-mail) imaishin@wustl.edu Accepted 2011-DEC-08 by JYOTI Aging Cell (1474-9718) 2011 mimix curation A nutrient-sensitive interaction between Sirt1 and HNF-1α regulates Crp expression. A nutrient-sensitive interaction between Sirt1 and HNF-1α regulates Crp expression. Aging Cell (1474-9718) 2011 mimix curation Grimm A.A., Brace C.S., Wang T., Stormo G.D., Imai S. Apoptosis - Interactions involving proteins with a function related to apoptosis Species origin of Sirt1 and HNF1alpha constructs confirmed by the authors (e-mail) imaishin@wustl.edu 14-Mar-2012: Contacted by JYOTI. human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Grimm A.A., Brace C.S., Wang T., Stormo G.D., Imai S. Apoptosis - Interactions involving proteins with a function related to apoptosis Species origin of Sirt1 and HNF1alpha constructs confirmed by the authors (e-mail) imaishin@wustl.edu Accepted 2011-DEC-08 by JYOTI 14-Mar-2012: Contacted by JYOTI. Aging Cell (1474-9718) 2011 mimix curation A nutrient-sensitive interaction between Sirt1 and HNF-1α regulates Crp expression. A nutrient-sensitive interaction between Sirt1 and HNF-1α regulates Crp expression. Aging Cell (1474-9718) 2011 mimix curation Grimm A.A., Brace C.S., Wang T., Stormo G.D., Imai S. Apoptosis - Interactions involving proteins with a function related to apoptosis Species origin of Sirt1 and HNF1alpha constructs confirmed by the authors (e-mail) imaishin@wustl.edu 14-Mar-2012: Contacted by JYOTI. mouse-liver Mus musculus liver liver anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Grimm A.A., Brace C.S., Wang T., Stormo G.D., Imai S. Apoptosis - Interactions involving proteins with a function related to apoptosis Species origin of Sirt1 and HNF1alpha constructs confirmed by the authors (e-mail) imaishin@wustl.edu Accepted 2011-DEC-08 by JYOTI 14-Mar-2012: Contacted by JYOTI. Aging Cell (1474-9718) 2011 mimix curation sir1_mouse NAD-dependent protein deacetylase sirtuin-1 NAD-dependent protein deacylase sirtuin-1 Sirt1 Sir2l1 SIR2alpha SIR2-like protein 1 Regulatory protein SIR2 homolog 1 protein protein mouse Mus musculus Mouse MADEVALALQAAGSPSAAAAMEAASQPADEPLRKRPRRDGPGLGRSPGEPSAAVAPAAAGCEAASAAAPAALWREAAGAAASAEREAPATAVAGDGDNGSGLRREPRAADDFDDDEGEEEDEAAAAAAAAAIGYRDNLLLTDGLLTNGFHSCESDDDDRTSHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRILQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRPQKELVHLSELPPTPLHISEDSSSPERTVPQDSSVIATLVDQATNNNVNDLEVSESSCVEEKPQEVQTSRNVENINVENPDFKAVGSSTADKNERTSVAETVRKCWPNRLAKEQISKRLEGNQYLFVPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPLEDESEIEEFYNGLEDDTERPECAGGSGFGADGGDQEVVNEAIATRQELTDVNYPSDKS DIP protein Q923E4 original sequence version: 83 DIP protein Q923E4 original sequence version: 135 7F15625E29433119 hnf1a_mouse Hepatocyte nuclear factor 1-alpha Hnf1a Hnf-1 Hnf-1a Tcf1 Liver-specific transcription factor LF-B1 Transcription factor 1 protein protein mouse Mus musculus Mouse MVSKLSQLQTELLAALLESGLSKEALIQALGEPGPYLMVGEGPLDKGESCGGSRGDLTELPNGLGETRGSEDDTDDDGEDFAPPILKELENLSPEEAAHQKAVVESLLQEDPWRVAKMVKSYLQQHNIPQREVVDTTGLNQSHLSQHLNKGTPMKTQKRAALYTWYVRKQREVAQQFTHAGQGGLIEEPTGDELPTKKGRRNRFKWGPASQQILFQAYERQKNPSKEERETLVEECNRAECIQRGVSPSQAQGLGSNLVTEVRVYNWFANRRKEEAFRHKLAMDTYNGPPPGPGPGPALPAHSSPGLPTTTLSPSKVHGVRYGQSATSEAAEVPSSSGGPLVTVSAALHQVSPTGLEPSSLLSTEAKLVSATGGPLPPVSTLTALHSLEQTSPGLNQQPQNLIMASLPGVMTIGPGEPASLGPTFTNTGASTLVIGLASTQAQSVPVINSMGSSLTTLQPVQFSQPLHPSYQQPLMPPVQSHVAQSPFMATMAQLQSPHALYSHKPEVAQYTHTSLLPQTMLITDTNLSTLASLTPTKQVFTSDTEASSEPGLHEPPSPATTIHIPSQDPSNIQHLQPAHRLSTSPTVSSSSLVLYQSSDSNGHSHLLPSNHSVIETFISTQMASSSQ 737920D47BA1F081 hnf1a-sirt1 2 6 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey direct interaction direct interaction 2A sirt1-hnf1a 1 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association 2b Low glucose and low serum hnf1a-sirt1-1 1 6 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association 2c, 3a, 3b and 3c Low glucose and low serum cycloheximide sirt1-hnf1a-1 4 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association 2d hnf1a-sirt1-2 3 6 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association S1