InnateDB InnateDB T-bet represses T(H)17 differentiation by preventing Runx1-mediated activation of the gene encoding RORγt. T-bet represses T(H)17 differentiation by preventing Runx1-mediated activation of the gene encoding RORγt. Nat. Immunol. (1529-2908) 2011 imex curation Lazarevic V., Chen X., Shim JH., Hwang ES., Jang E., Bolm AN., Oukka M., Kuchroo VK., Glimcher LH. Th0 cells taken from wt and tbx21 KO mice lglimche@hsph.harvard.edu 06-Jun-2011: Contacted by JYOTI. mgt Only protein-protein interactions Curated by: InnateDB - a knowledge resource for innate immunity mouse-t-cell Mouse T cell t-cell Peripheral blood T-lymphocytes anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lazarevic V., Chen X., Shim JH., Hwang ES., Jang E., Bolm AN., Oukka M., Kuchroo VK., Glimcher LH. Th0 cells taken from wt and tbx21 KO mice lglimche@hsph.harvard.edu Accepted 2011-APR-04 by ORCHARD 06-Jun-2011: Contacted by JYOTI. Nat. Immunol. (1529-2908) 2011 mgt Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity T-bet represses T(H)17 differentiation by preventing Runx1-mediated activation of the gene encoding RORγt. T-bet represses T(H)17 differentiation by preventing Runx1-mediated activation of the gene encoding RORγt. Nat. Immunol. (1529-2908) 2011 imex curation Lazarevic V., Chen X., Shim JH., Hwang ES., Jang E., Bolm AN., Oukka M., Kuchroo VK., Glimcher LH. Th0 cells taken from wt and tbx21 KO mice lglimche@hsph.harvard.edu 06-Jun-2011: Contacted by JYOTI. mgt Only protein-protein interactions Curated by: InnateDB - a knowledge resource for innate immunity human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Lazarevic V., Chen X., Shim JH., Hwang ES., Jang E., Bolm AN., Oukka M., Kuchroo VK., Glimcher LH. Th0 cells taken from wt and tbx21 KO mice Source of Tbx21 inferred from mutant mapping, source of Runx1 from reference given by contributing laboratory. Species of Runx1, Runx3 confirmed through correspondence. lglimche@hsph.harvard.edu Accepted 2011-APR-04 by ORCHARD 06-Jun-2011: Contacted by JYOTI. Nat. Immunol. (1529-2908) 2011 mgt Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity runx3_mouse Runt-related transcription factor 3 Runx3 Aml2 Cbfa3 Pebp2a3 Acute myeloid leukemia 2 protein Core-binding factor subunit alpha-3 Oncogene AML-2 Polyomavirus enhancer-binding protein 2 alpha C subunit SL3-3 enhancer factor 1 alpha C subunit SL3/AKV core-binding factor alpha C subunit protein protein mouse Mus musculus Mouse MRIPVDPSTSRRFTPPSTAFPCGGGGGGKMGENSGALSAQATAGPGGRTRPEVRSMVDVLADHAGELVRTDSPNFLCSVLPSHWRCNKTLPVAFKVVALGDVPDGTVVTVMAGNDENYSAELRNASAVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPTQVATYHRAIKVTVDGPREPRRHRQKIEDQTKAFPDRFGDLRMRVTPSTPSPRGSLSTTSHFSSQAQTPIQGSSDLNPFSDPRQFDRSFPTLQSLTESRFPDPRMHYPGAMSAAFPYSATPSGTSLGSLSVAGMPASSRFHHTYLPPPYPGAPQSQSGPFQANPAPYHLFYGASSGSYQFSMAAAGGGERSPTRMLTSCPSGASVSAGNLMNPSLGQADGVEADGSHSNSPTALSTPGRMDEAVWRPY D2BCEBAF808A315D runx1_mouse Runt-related transcription factor 1 Runx1 Aml1 Cbfa2 Pebp2ab Acute myeloid leukemia 1 protein Core-binding factor subunit alpha-2 Oncogene AML-1 Polyomavirus enhancer-binding protein 2 alpha B subunit SL3-3 enhancer factor 1 alpha B subunit SL3/AKV core-binding factor alpha B subunit protein protein mouse Mus musculus Mouse MRIPVDASTSRRFTPPSTALSPGKMSEALPLGAPDGGPALASKLRSGDRSMVEVLADHPGELVRTDSPNFLCSVLPTHWRCNKTLPIAFKVVALGDVPDGTLVTVMAGNDENYSAELRNATAAMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPPQVATYHRAIKITVDGPREPRRHRQKLDDQTKPGSLSFSERLSELEQLRRTAMRVSPHHPAPTPNPRASLNHSTAFNPQPQSQMQDARQIQPSPPWSYDQSYQYLGSITSSSVHPATPISPGRASGMTSLSAELSSRLSTAPDLTAFGDPRQFPTLPSISDPRMHYPGAFTYSPPVTSGIGIGMSAMSSASRYHTYLPPPYPGSSQAQAGPFQTGSPSYHLYYGASAGSYQFSMVGGERSPPRILPPCTNASTGAALLNPSLPSQSDVVETEGSHSNSPTNMPPARLEEAVWRPY DIP protein Q03347 original sequence version: 136 DIP protein Q03347 original sequence version: 128 tbx21_mouse T-box transcription factor TBX21 Tbx21 Tbet Tblym T-cell-specific T-box transcription factor T-bet Transcription factor TBLYM protein protein mouse Mus musculus Mouse MGIVEPGCGDMLTGTEPMPSDEGRGPGADQQHRFFYPEPGAQDPTDRRAGSSLGTPYSGGALVPAAPGRFLGSFAYPPRAQVAGFPGPGEFFPPPAGAEGYPPVDGYPAPDPRAGLYPGPREDYALPAGLEVSGKLRVALSNHLLWSKFNQHQTEMIITKQGRRMFPFLSFTVAGLEPTSHYRMFVDVVLVDQHHWRYQSGKWVQCGKAEGSMPGNRLYVHPDSPNTGAHWMRQEVSFGKLKLTNNKGASNNVTQMIVLQSLHKYQPRLHIVEVNDGEPEAACSASNTHVFTFQETQFIAVTAYQNAEITQLKIDNNPFAKGFRENFESMYASVDTSVPSPPGPNCQLLGGDPFSPLLSNQYPVPSRFYPDLPGQPKDMISQPYWLGTPREHSYEAEFRAVSMKPTLLPSAPGPTVPYYRGQDVLAPGAGWPVAPQYPPKMSPAGWFRPMRTLPMDPGLGSSEEQGSSPSLWPEVTSLQPEPSDSGLGEGDTKRRRISPYPSSGDSSSPAGAPSPFDKETEGQFYNYFPN tbx21-runx3 2 3 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait physical association physical association Fig. 5A tbx21-runx1-4 2 5 unspecified role unspecified role bait bait Q9JKD8:p.Tyr304Phe mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 17:28:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr304phe' Tue Jun 30 19:43:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr304Phe' 4 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 5A, 7A runx1-tbx21 1 4 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Fig. 5C tbx21-runx1-3 1 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association Fig. 5C