MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it A positively charged channel within the Smc1/Smc3 hinge required for sister chromatid cohesion. A positively charged channel within the Smc1/Smc3 hinge required for sister chromatid cohesion. EMBO J. (0261-4189) 2011 imex curation Kurze A., Michie KA., Dixon SE., Mishra A., Itoh T., Khalid S., Strmecki L., Shirahige K., Haering CH., Löwe J., Nasmyth K. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Kurze A., Michie KA., Dixon SE., Mishra A., Itoh T., Khalid S., Strmecki L., Shirahige K., Haering CH., Löwe J., Nasmyth K. EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation A positively charged channel within the Smc1/Smc3 hinge required for sister chromatid cohesion. A positively charged channel within the Smc1/Smc3 hinge required for sister chromatid cohesion. EMBO J. (0261-4189) 2011 imex curation Kurze A., Michie KA., Dixon SE., Mishra A., Itoh T., Khalid S., Strmecki L., Shirahige K., Haering CH., Löwe J., Nasmyth K. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Kurze A., Michie KA., Dixon SE., Mishra A., Itoh T., Khalid S., Strmecki L., Shirahige K., Haering CH., Löwe J., Nasmyth K. EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation A positively charged channel within the Smc1/Smc3 hinge required for sister chromatid cohesion. A positively charged channel within the Smc1/Smc3 hinge required for sister chromatid cohesion. EMBO J. (0261-4189) 2011 imex curation Kurze A., Michie KA., Dixon SE., Mishra A., Itoh T., Khalid S., Strmecki L., Shirahige K., Haering CH., Löwe J., Nasmyth K. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Kurze A., Michie KA., Dixon SE., Mishra A., Itoh T., Khalid S., Strmecki L., Shirahige K., Haering CH., Löwe J., Nasmyth K. EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation A positively charged channel within the Smc1/Smc3 hinge required for sister chromatid cohesion. A positively charged channel within the Smc1/Smc3 hinge required for sister chromatid cohesion. EMBO J. (0261-4189) 2011 imex curation Kurze A., Michie KA., Dixon SE., Mishra A., Itoh T., Khalid S., Strmecki L., Shirahige K., Haering CH., Löwe J., Nasmyth K. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kurze A., Michie KA., Dixon SE., Mishra A., Itoh T., Khalid S., Strmecki L., Shirahige K., Haering CH., Löwe J., Nasmyth K. EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation A positively charged channel within the Smc1/Smc3 hinge required for sister chromatid cohesion. A positively charged channel within the Smc1/Smc3 hinge required for sister chromatid cohesion. EMBO J. (0261-4189) 2011 imex curation Kurze A., Michie KA., Dixon SE., Mishra A., Itoh T., Khalid S., Strmecki L., Shirahige K., Haering CH., Löwe J., Nasmyth K. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Kurze A., Michie KA., Dixon SE., Mishra A., Itoh T., Khalid S., Strmecki L., Shirahige K., Haering CH., Löwe J., Nasmyth K. EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation pds5_yeast Sister chromatid cohesion protein PDS5 PDS5 YMR076C YM9916.15C YM9582.01C Precocious dissociation of sisters protein 5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MAKGAVTKLKFNSPIISTSDQLISTNELLDRLKALHEELASLDQDNTDLTGLDKYRDALVSRKLLKHKDVGIRAFTACCLSDILRLYAPDAPYTDAQLTDIFKLVLSQFEQLGDQENGYHIQQTYLITKLLEYRSIVLLADLPSSNNLLIELFHIFYDPNKSFPARLFNVIGGILGEVISEFDSVPLEVLRLIFNKFLTYNPNEIPEGLNVTSDCGYEVSLILCDTYSNRMSRHLTKYYSEIIHEATNDDNNSRLLTVVVKLHKLVLRLWETVPELINAVIGFIYHELSSENELFRKEATKLIGQILTSYSDLNFVSTHSDTFKAWISKIADISPDVRVEWTESIPQIIATREDISKELNQALAKTFIDSDPRVRRTSVMIFNKVPVTEIWKNITNKAIYTSLLHLAREKHKEVRELCINTMAKFYSNSLNEIERTYQNKEIWEIIDTIPSTLYNLYYINDLNINEQVDSVIFEYLLPFEPDNDKRVHRLLTVLSHFDKKAFTSFFAFNARQIKISFAISKYIDFSKFLNNQESMSSSQGPIVMNKYNQTLQWLASGLSDSTKAIDALETIKQFNDERIFYLLNACVTNDIPFLTFKNCYNELVSKLQTPGLFKKYNISTGASIMPRDIAKVIQILLFRASPIIYNVSNISVLLNLSNNSDAKQLDLKRRILDDISKVNPTLFKDQIRTLKTIIKDLDDPDAEKNDNLSLEEALKTLYKASKTLKDQVDFDDTFFFTKLYDFAVESKPEITKYATKLIALSPKAEETLKKIKIRILPLDLQKDKYFTSHIIVLMEIFKKFPHVLNDDSTDIISYLIKEVLLSNQVVGDSKKEIDWVEDSLLSDTKYSAIGNKVFTLKLFTNKLRSIAPDVPRDELAESFTEKTMKLFFYLIASGGELISEFNKEFYPTPSNYQTKLRCVAGIQVLKLARISNLNNFIKPSDIIKLINLVEDESLPVRKTFLEQLKDYVANELISIKFLPLVFFTAYEPDVELKTTTKIWINFTFGLKSFKKGTIFERALPRLIHAIAHHPDIVGGLDSEGDAYLNALTTAIDYLLFYFDSIAAQENFSLLYYLSERVKNYQDKLVEDEIDEEEGPQKEEAPKKHRPYGQKMYIIGELSQMILLNLKEKKNWQHSAYPGKLNLPSDLFKPFATVQEAQLSFKTYIPESLTEKIQNNIKAKIGRILHTSQTQRQRLQKRLLAHENNESQKKKKKVHHARSQADDEEGDGDRESDSDDDSYSPSNKNETKKGHENIVMKKLRVRKEVDYKDDEDDDIEMT 9DF40A5274FD9623 scc1_yeast Sister chromatid cohesion protein 1 MCD1 PDS3 RHC21 SCC1 YDL003W YD8119.04 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVTENPQRLTVLRLATNKGPLAQIWLASNMSNIPRGSVIQTHIAESAKEIAKASGCDDESGDNEYITLRTSGELLQGIVRVYSKQATFLLTDIKDTLTKISMLFKTSQKMTSTVNRLNTVTRVHQLMLEDAVTEREVLVTPGLEFLDDTTIPVGLMAQENSMERKVQGAAPWDTSLEVGRRFSPDEDFEHNNLSSMNLDFDIEEGPITSKSWEEGTRQSSRNFDTHENYIQDDDFPLDDAGTIGWDLGITEKNDQNNDDDDNSVEQGRRLGESIMSEEPTDFGFDLDIEKEAPAGNIDTITDAMTESQPKQTGTRRNSKLLNTKSIQIDEETENSESIASSNTYKEERSNNLLTPQPTNFTTKRLWSEITESMSYLPDPILKNFLSYESLKKRKIHNGREGSIEEPELNVSLNLTDDVISNAGTNDNSFNELTDNMSDFVPIDAGLNEAPFPEENIIDAKTRNEQTTIQTEKVRPTPGEVASKAIVQMAKILRKELSEEKEVIFTDVLKSQANTEPENITKREASRGFFDILSLATEGCIGLSQTEAFGNIKIDAKPALFERFINA EB6C7CA33BAC208F EB6C7CA33BAC208F smc3_yeast Structural maintenance of chromosomes protein 3 SMC3 YJL074C J1049 DA-box protein SMC3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MYIKRVIIKGFKTYRNETIIDNFSPHQNVIIGSNGSGKSNFFAAIRFVLSDDYSNLKREERQGLIHQGSGGSVMSASVEIVFHDPDHSMILPSGVLSRGDDEVTIRRTVGLKKDDYQLNDRNVTKGDIVRMLETAGFSMNNPYNIVPQGKIVALTNAKDKERLQLLEDVVGAKSFEVKLKASLKKMEETEQKKIQINKEMGELNSKLSEMEQERKELEKYNELERNRKIYQFTLYDRELNEVINQMERLDGDYNNTVYSSEQYIQELDKREDMIDQVSKKLSSIEASLKIKNATDLQQAKLRESEISQKLTNVNVKIKDVQQQIESNEEQRNLDSATLKEIKSIIEQRKQKLSKILPRYQELTKEEAMYKLQLASLQQKQRDLILKKGEYARFKSKDERDTWIHSEIEELKSSIQNLNELESQLQMDRTSLRKQYSAIDEEIEELIDSINGPDTKGQLEDFDSELIHLKQKLSESLDTRKELWRKEQKLQTVLETLLSDVNQNQRNVNETMSRSLANGIINVKEITEKLKISPESVFGTLGELIKVNDKYKTCAEVIGGNSLFHIVVDTEETATLIMNELYRMKGGRVTFIPLNRLSLDSDVKFPSNTTTQIQFTPLIKKIKYEPRFEKAVKHVFGKTIVVKDLGQGLKLAKKHKLNAITLDGDRADKRGVLTGGYLDQHKRTRLESLKNLNESRSQHKKILEELDFVRNELNDIDTKIDQVNGNIRKVSNDRESVLTNIEVYRTSLNTKKNEKLILEESLNAIILKLEKLNTNRTFAQEKLNTFENDLLQEFDSELSKEEKERLESLTKEISAAHNKLNITSDALEGITTTIDSLNAELESKLIPQENDLESKMSEVGDAFIFGLQDELKELQLEKESVEKQHENAVLELGTVQREIESLIAEETNNKKLLEKANNQQRLLLKKLDNFQKSVEKTMIKKTTLVTRREELQQRIREIGLLPEDALVNDFSDITSDQLLQRLNDMNTEISGLKNVNKRAFENFKKFNERRKDLAERASELDESKDSIQDLIVKLKQQKVNAVDSTFQKVSENFEAVFERLVPRGTAKLIIHRKNDNANDHDESIDVDMDAESNESQNGKDSEIMYTGVSISVSFNSKQNEQLHVEQLSGGQKTVCAIALILAIQMVDPASFYLFDEIDAALDKQYRTAVATLLKELSKNAQFICTTFRTDMLQVADKFFRVKYENKISTVIEVNREEAIGFIRGSNKFAEV B152D88F7780341F B152D88F7780341F smc1_yeast Structural maintenance of chromosomes protein 1 YFL008W SMC1 CHL10 DA-box protein SMC1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MGRLVGLELSNFKSYRGVTKVGFGESNFTSIIGPNGSGKSNMMDAISFVLGVRSNHLRSNILKDLIYRGVLNDENSDDYDNEGAASSNPQSAYVKAFYQKGNKLVELMRIISRNGDTSYKIDGKTVSYKDYSIFLENENILIKAKNFLVFQGDVEQIAAQSPVELSRMFEEVSGSIQYKKEYEELKEKIEKLSKSATESIKNRRRIHGELKTYKEGINKNEEYRKQLDKKNELQKFQALWQLYHLEQQKEELTDKLSALNSEISSLKGKINNEMKSLQRSKSSFVKESAVISKQKSKLDYIFKDKEKLVSDLRLIKVPQQAAGKRISHIEKRIESLQKDLQRQKTYVERFETQLKVVTRSKEAFEEEIKQSARNYDKFKLNENDLKTYNCLHEKYLTEGGSILEEKIAVLNNDKREIQEELERFNKRADISKRRITEELSITGEKLDTQLNDLRVSLNEKNALHTERLHELKKLQSDIESANNQEYDLNFKLRETLVKIDDLSANQRETMKERKLRENIAMLKRFFPGVKGLVHDLCHPKKEKYGLAVSTILGKNFDSVIVENLTVAQECIAFLKKQRAGTASFIPLDTIETELPTLSLPDSQDYILSINAIDYEPEYEKAMQYVCGDSIICNTLNIAKDLKWKKGIRGKLVTIEGALIHKAGLMTGGISGDANNRWDKEEYQSLMSLKDKLLIQIDELSNGQRSNSIRAREVENSVSLLNSDIANLRTQVTQQKRSLDENRLEIKYHNDLIEKEIQPKITELKKKLDDLENTKDNLVKEKEALQNNIFKEFTSKIGFTIKEYENHSGELMRQQSKELQQLQKQILTVENKLQFETDRLSTTQRRYEKAQKDLENAQVEMKSLEEQEYAIEMKIGSIESKLEEHKNHLDELQKKFVTKQSELNSSEDILEDMNSNLQVLKRERDGIKEDIEKFDLERVTALKNCKISNINIPISSETTIDDLPISSTDNEAITISNSIDINYKGLPKKYKENNTDSARKELEQKIHEVEEILNELQPNARALERYDEAEGRFEVINNETEQLKAEEKKILNQFLKIKKKRKELFEKTFDYVSDHLDAIYRELTKNPNSNVELAGGNASLTIEDEDEPFNAGIKYHATPPLKRFKDMEYLSGGEKTVAALALLFAINSYQPSPFFVLDEVDAALDITNVQRIAAYIRRHRNPDLQFIVISLKNTMFEKSDALVGVYRQQQENSSKIITLDLSNYAE B504017AA0ECCA8C B504017AA0ECCA8C smc3_mouse Structural maintenance of chromosomes protein 3 Smc3 Bam Bmh Cspg6 Mmip1 Smc3l1 Smcd Chondroitin sulfate proteoglycan 6 Chromosome segregation protein SmcD Basement membrane-associated chondroitin proteoglycan Mad member-interacting protein 1 protein protein mouse Mus musculus Mouse MYIKQVIIQGFRSYRDQTIVDPFSSKHNVIVGRNGSGKSNFFYAIQFVLSDEFSHLRPEQRLALLHEGTGPRVISAFVEIIFDNSDNRLPIDKEEVSLRRVIGAKKDQYFLDKKMVTKNDVMNLLESAGFSRSNPYYIVKQGKINQMATAPDSQRLKLLREVAGTRVYDERKEESISLMKETEGKREKINELLKYIEERLHTLEEEKEELAQYQKWDKMRRALEYTIYNQELNETRAKLDELSAKRETSGEKSRQLRDAQQDARDKMEDIERQVRELKTKISAMKEEKEQLSAERQEQIKQRTKLELKAKDLQDELAGNSEQRKRLLKERQKLLEKIEEKQKELAETEPKFNSVKEKEERGIARLAQATQERTDLYAKQGRGSQFTSKEERDKWIKKELKSLDQAINDKKRQIAAIHKDLEDTEANKEKNLEQYNKLDQDLNEVKARVEELDRKYYEVKNKKDELQSERNYLWREENAEQQALAAKREDLEKKQQLLRAATGKAILNGIDSINKVLEHFRRKGINQHVQNGYHGIVMNNFECEPAFYTCVEVTAGNRLFYHIVDSDEVSTKILMEFNKMNLPGEVTFLPLNKLDVRDTAYPETNDAIPMISKLRYNPRFDKAFKHVFGKTLICRSMEVSTQLARAFTMDCITLEGDQVSHRGALTGGYYDTRKSRLELQKDVRKAEEELGELEAKLNENLRRNIERINNEIDQLMNQMQQIETQQRKFKASRDSILSEMKMLKEKRQQSEKTFMPKQRSLQSLEASLHAMESTRESLKAELGTDLLSQLSLEDQKRVDALNDEIRQLQQENRQLLNERIKLEGIITRVETYLNENLRKRLDQVEQELNELRETEGGTVLTATTSELEAINKRVKDTMARSEDLDNSIDKTEAGIKELQKSMERWKNMEKEHMDAINHDTKELEKMTNRQGMLLKKKEECMKKIRELGSLPQEAFEKYQTLSLKQLFRKLEQCNTELKKYSHVNKKALDQFVNFSEQKEKLIKRQEELDRGYKSIMELMNVLELRKYEAIQLTFKQVSKNFSEVFQKLVPGGKATLVMKKGDVEGSQSQDEGEGSGESERGSGSQSSVPSVDQFTGVGIRVSFTGKQGEMREMQQLSGGQKSLVALALIFAIQKCDPAPFYLFDEIDQALDAQHRKAVSDMIMELAVHAQFITTTFRPELLESADKFYGVKFRNKVSHIDVITAEMAKDFVEDDTTHG DIP protein Q9CW03 original sequence version: 100 DIP protein Q9CW03 original sequence version: 80 DIP protein Q9CW03 original sequence version: 115 11FF55165BE6A88E smc1a_mouse Structural maintenance of chromosomes protein 1A Smc1a Smc1 Smc1l1 Smcb Sb1.8 Chromosome segregation protein SmcB protein protein mouse Mus musculus Mouse MGFLKLIEIENFKSYKGRQIIGPFQRFTAIIGPNGSGKSNLMDAISFVLGEKTSNLRVKTLRDLIHGAPVGKPAANRAFVSMVYSEEGAEDRTFARVIVGGSSEYKINNKVVQLHEYSEELEKLGILIKARNFLVFQGAVESIAMKNPKERTALFEEISRSGELAQEYDKRKKEMVKAEEDTQFNYHRKKNIAAERKEAKQEKEEADRYQRLKDEVVRAQVQLQLFKLYHNEVEIEKLNKELASKNKEIEKDKKRMDKVEDELKEKKKELGKMMREQQQIEKEIKEKDSELNQKRPQYIKAKENTSHKIKKLEAAKKSLQNAQKHYKKRKGDMDELEKEMLSVEKARQEFEERMEEESQSQGRDLTLEENQVKKYHRLKEEASKRAATLAQELEKFNRDQKADQDRLDLEERKKVETEAKIKQKLREIEENQKRIEKLEEYITTSKQSLEEQKKLEGELTEEVEMAKRRIDEINKELNQVMEQLGDARIDRQESSRQQRKAEIMESIKRLYPGSVYGRLIDLCQPTQKKYQIAVTKVLGKNMDAIIVDSEKTGRDCIQYIKEQRGEPETFLPLDYLEVKPTDEKLRELKGAKLVIDVIRYEPPHIKKALQYACGNALVCDNVEDARRIAFGGHQRHKTVALDGTLFQKSGVISGGASDLKAKARRWDEKAVDKLKEKKERLTEELKEQMKAKRKEAELRQVQSQAHGLQMRLKYSQSDLEQTKTRHLALNLQEKSKLESELANFGPRINDIKRIIQSREREMKDLKEKMNQVEDEVFEEFCREIGVRNIREFEEEKVKRQNEIAKKRLEFENQKTRLGIQLDFEKNQLKEDQDKVHMWEQTVKKDENEIEKLKKEEQRHMKIIDETMAQLQDLKNQHLAKKSEVNDKNHEMEEIRKKLGGANKEMTHLQKEVTAIETKLEQKRSDRHNLLQACKMQDIKLPLSKGTMDDISQEEGSSQGEESVSGSQRTSSIYAREALIEIDYGDLCEDLKDAQAEEEIKQEMNTLQQKLNEQQSVLQRIAAPNMKAMEKLESVRDKFQETSDEFEAARKRAKKAKQAFEQIKKERFDRFNACFESVATNIDEIYKALSRNSSAQAFLGPENPEEPYLDGINYNCVAPGKRFRPMDNLSGGEKTVAALALLFAIHSYKPAPFFVLDEIDAALDNTNIGKVANYIKEQSTCNFQAIVISLKEEFYTKAESLIGVYPEQGDCVISKVLTFDLTKYPDANPNPNEQ DIP protein Q9CU62 original sequence version: 82 E62CEB174854D9A6 smc1a-smc3 3 Smc3 10 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Smc1 11 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-8536311]The shifted ranges (471-685) couldn't be applied as the new full feature sequence is different from the previous one. [EBI-8536311]471-685 [EBI-8536311]Q9CU62,3 direct interaction direct interaction homomint mint The resulting structure was refined to 2.7A° (Supplementary Table 1). The crystals contained one heterodimer of the Smc1/Smc3 complex, ordered between residues 499 and 675 for Smc1 (chain A), and residues 492 and 670, and 674 and 685 for Smc3 (chain B). st1 st2 f1 smc1-smc3-14 1 SMC1 9 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified SMC3 8 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction f3b homomint mint smc1-smc3-15 1 SMC3 8 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified P47037:p.Arg665Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Tue Jul 12 10:07:05 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 15:53:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg665ala' Tue Jun 30 19:13:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg665Ala' P47037:p.Arg669Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing protein footprinting protein footprinting certain certain sequence position certain certain certain sequence position certain R A Tue Jul 12 10:07:28 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 15:08:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg669ala' Tue Jun 30 19:46:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg669Ala' P47037:p.Lys668Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A Tue Jul 12 10:50:08 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 17:42:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys668ala' Tue Jun 30 19:13:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys668Ala' SMC1 9 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified P32908:p.Lys661Asp mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K D Tue Jul 12 10:07:09 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 20:13:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys661asp' Tue Jun 30 16:32:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys661Asp' P32908:p.Lys554Asp mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K D Tue Jul 12 10:49:54 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 19:07:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys554asp' Tue Jun 30 16:59:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys554Asp' direct interaction direct interaction f3b homomint mint smc3-mcd1-4 4 SCC1 7 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined SMC3 8 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined SMC1 9 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association homomint mint sf5b smc3-pds5-5 4 PDS5 6 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined SMC3 8 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association homomint mint sf5c smc3-smc1-9 5 SMC1 9 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified SMC3 8 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction homomint mint f4b f4c f4d f4e smc3-smc1-10 5 SMC3 8 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined SMC1 9 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified P32908:p.Met665Arg mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain M R Tue Jul 12 10:05:19 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 17:51:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met665arg' Tue Jun 30 18:44:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met665Arg' direct interaction direct interaction f4f homomint mint smc1-smc3-16 2 SMC3 8 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified SMC1 9 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction f3a homomint mint