IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Rb-Raf-1 interaction disruptor RRD-251 induces apoptosis in metastatic melanoma cells and synergizes with dacarbazine. Rb-Raf-1 interaction disruptor RRD-251 induces apoptosis in metastatic melanoma cells and synergizes with dacarbazine. Mol. Cancer Ther. (1535-7163) 2010 imex curation Singh S., Davis R., Alamanda V., Pireddu R., Pernazza D., Sebti S., Lawrence N., Chellappan S. The cells were xenotransplanted to create tumors in athymic nude mice. The tumors were extracted after treatment with DMSO or RD-251 and this tissue was used to perform the coinmunoprecipitation. Srikumar.Chellappan@moffitt.org 23-Sept-2011: Contacted by JYOTI. Only protein-protein interactions human-sk_mel_28 Homo sapiens SK-MEL-28 cell line derived from melanoma sk_mel_28 human SK-MEL-28 Species: human male 51 years old; Tissue: skin; Tumor: melanoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Singh S., Davis R., Alamanda V., Pireddu R., Pernazza D., Sebti S., Lawrence N., Chellappan S. The cells were xenotransplanted to create tumors in athymic nude mice. The tumors were extracted after treatment with DMSO or RD-251 and this tissue was used to perform the coinmunoprecipitation. Srikumar.Chellappan@moffitt.org Accepted 2011-AUG-23 by ORCHARD 23-Sept-2011: Contacted by JYOTI. Mol. Cancer Ther. (1535-7163) 2010 Only protein-protein interactions imex curation Rb-Raf-1 interaction disruptor RRD-251 induces apoptosis in metastatic melanoma cells and synergizes with dacarbazine. Rb-Raf-1 interaction disruptor RRD-251 induces apoptosis in metastatic melanoma cells and synergizes with dacarbazine. Mol. Cancer Ther. (1535-7163) 2010 imex curation Singh S., Davis R., Alamanda V., Pireddu R., Pernazza D., Sebti S., Lawrence N., Chellappan S. The cells were xenotransplanted to create tumors in athymic nude mice. The tumors were extracted after treatment with DMSO or RD-251 and this tissue was used to perform the coinmunoprecipitation. Srikumar.Chellappan@moffitt.org 23-Sept-2011: Contacted by JYOTI. Only protein-protein interactions human-sk_mel_28 Homo sapiens SK-MEL-28 cell line derived from melanoma sk_mel_28 human SK-MEL-28 Species: human male 51 years old; Tissue: skin; Tumor: melanoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Singh S., Davis R., Alamanda V., Pireddu R., Pernazza D., Sebti S., Lawrence N., Chellappan S. The cells were xenotransplanted to create tumors in athymic nude mice. The tumors were extracted after treatment with DMSO or RD-251 and this tissue was used to perform the coinmunoprecipitation. Srikumar.Chellappan@moffitt.org Accepted 2011-AUG-23 by ORCHARD 23-Sept-2011: Contacted by JYOTI. Mol. Cancer Ther. (1535-7163) 2010 Only protein-protein interactions imex curation Rb-Raf-1 interaction disruptor RRD-251 induces apoptosis in metastatic melanoma cells and synergizes with dacarbazine. Rb-Raf-1 interaction disruptor RRD-251 induces apoptosis in metastatic melanoma cells and synergizes with dacarbazine. Mol. Cancer Ther. (1535-7163) 2010 imex curation Singh S., Davis R., Alamanda V., Pireddu R., Pernazza D., Sebti S., Lawrence N., Chellappan S. The cells were xenotransplanted to create tumors in athymic nude mice. The tumors were extracted after treatment with DMSO or RD-251 and this tissue was used to perform the coinmunoprecipitation. Srikumar.Chellappan@moffitt.org 23-Sept-2011: Contacted by JYOTI. Only protein-protein interactions human-sk_mel_2 Human Skin Melanoma cell line 2 sk-mel-2 Human Skin Melanoma cell line 2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Singh S., Davis R., Alamanda V., Pireddu R., Pernazza D., Sebti S., Lawrence N., Chellappan S. The cells were xenotransplanted to create tumors in athymic nude mice. The tumors were extracted after treatment with DMSO or RD-251 and this tissue was used to perform the coinmunoprecipitation. Srikumar.Chellappan@moffitt.org Accepted 2011-AUG-23 by ORCHARD 23-Sept-2011: Contacted by JYOTI. Mol. Cancer Ther. (1535-7163) 2010 Only protein-protein interactions imex curation rb_human Retinoblastoma-associated protein pRb pp110 p105-Rb RB1 p110-RB1 protein protein human Homo sapiens Human MPPKTPRKTAATAAAAAAEPPAPPPPPPPEEDPEQDSGPEDLPLVRLEFEETEEPDFTALCQKLKIPDHVRERAWLTWEKVSSVDGVLGGYIQKKKELWGICIFIAAVDLDEMSFTFTELQKNIEISVHKFFNLLKEIDTSTKVDNAMSRLLKKYDVLFALFSKLERTCELIYLTQPSSSISTEINSALVLKVSWITFLLAKGEVLQMEDDLVISFQLMLCVLDYFIKLSPPMLLKEPYKTAVIPINGSPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNIDEVKNVYFKNFIPFMNSLGLVTSNGLPEVENLSKRYEEIYLKNKDLDARLFLDHDKTLQTDSIDSFETQRTPRKSNLDEEVNVIPPHTPVRTVMNTIQQLMMILNSASDQPSENLISYFNNCTVNPKESILKRVKDIGYIFKEKFAKAVGQGCVEIGSQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSTSQNLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKAEGNLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDREGPTDHLESACPLNLPLQNNHTAADMYLSPVRSPKKKGSTTRVNSTANAETQATSAFQTQKPLKSTSLSLFYKKVYRLAYLRLNTLCERLLSEHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAVQETFKRVLIKEEEYDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFPSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGSNPPKPLKKLRFDIEGSDEADGSKHLPGESKFQQKLAEMTSTRTRMQKQKMNDSMDTSNKEEK DIP protein P06400 original sequence version: 102 DIP protein P06400 original sequence version: 195 DIP protein P06400 original sequence version: 204 DIP protein P06400 original sequence version: 126 DIP protein P06400 original sequence version: 151 DIP protein P06400 original sequence version: 153 DIP protein P06400 original sequence version: 181 DIP protein P06400 original sequence version: 184 DIP protein P06400 original sequence version: 213 DIP protein P06400 original sequence version: 208 DIP protein P06400 original sequence version: 174 DIP protein P06400 original sequence version: 212 DIP protein P06400 original sequence version: 179 DIP protein P06400 original sequence version: 146 C8E746111E19CC32 raf1_human RAF proto-oncogene serine/threonine-protein kinase RAF1 RAF Proto-oncogene c-RAF Raf-1 protein protein human Homo sapiens Human MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF DIP protein P04049 original sequence version: 117 DIP protein P04049 original sequence version: 106 DIP protein P04049 original sequence version: 114 DIP protein P04049 original sequence version: 141 DIP protein P04049 original sequence version: 142 DIP protein P04049 original sequence version: 147 DIP protein P04049-1 original sequence version: 136 DIP protein P04049 original sequence version: 136 DIP protein P04049 original sequence version: 153 DIP protein P04049 original sequence version: 150 DIP protein P04049 original sequence version: 164 DIP protein P04049 original sequence version: 172 DIP protein P04049-1 original sequence version: 172 DIP protein P04049 original sequence version: 184 DIP protein P04049 original sequence version: 188 DIP protein P04049 original sequence version: 195 EF821B5349711BC3 rb1-raf1 2 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association 2b Small molecule RD-251 decreases interaction. raf1-rb1 1 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association 2c Small molecule RD-251 decreases interaction. raf1-rb1-1 3 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association 2b Small molecule RD-251 decreases the interaction.