InnateDB InnateDB HLA-DM captures partially empty HLA-DR molecules for catalyzed removal of peptide. HLA-DM captures partially empty HLA-DR molecules for catalyzed removal of peptide. Nat. Immunol. (1529-2908) 2011 imex curation Anders AK., Call MJ., Schulze MS., Fowler KD., Schubert DA., Seth NP., Sundberg EJ., Wucherpfennig KW. kai_wucherpfennig@dfci.harvard.edu 06-Jun-2011: Contacted by JYOTI. mgt Only protein-protein interactions Curated by: InnateDB - a knowledge resource for innate immunity in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Anders AK., Call MJ., Schulze MS., Fowler KD., Schubert DA., Seth NP., Sundberg EJ., Wucherpfennig KW. kai_wucherpfennig@dfci.harvard.edu Accepted 2011-APR-04 by ORCHARD 06-Jun-2011: Contacted by JYOTI. Nat. Immunol. (1529-2908) 2011 mgt Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity HLA-DM captures partially empty HLA-DR molecules for catalyzed removal of peptide. HLA-DM captures partially empty HLA-DR molecules for catalyzed removal of peptide. Nat. Immunol. (1529-2908) 2011 imex curation Anders AK., Call MJ., Schulze MS., Fowler KD., Schubert DA., Seth NP., Sundberg EJ., Wucherpfennig KW. kai_wucherpfennig@dfci.harvard.edu 06-Jun-2011: Contacted by JYOTI. mgt Only protein-protein interactions Curated by: InnateDB - a knowledge resource for innate immunity in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Anders AK., Call MJ., Schulze MS., Fowler KD., Schubert DA., Seth NP., Sundberg EJ., Wucherpfennig KW. Re: Protein C tag on HLA-DMB. In methods, author stated HLA-DMB was tagged with Protein C on the extracellular domain (19..19-218..218 according to Uniprotkb) kai_wucherpfennig@dfci.harvard.edu Accepted 2011-APR-04 by ORCHARD 06-Jun-2011: Contacted by JYOTI. Nat. Immunol. (1529-2908) 2011 mgt Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity mbp_human Myelin basic protein MBP Myelin A1 protein Myelin membrane encephalitogenic protein protein protein human Homo sapiens Human MGNHAGKRELNAEKASTNSETNRGESEKKRNLGELSRTTSEDNEVFGEADANQNNGTSSQDTAVTDSKRTADPKNAWQDAHPADPGSRPHLIRLFSRDAPGREDNTFKDRPSESDELQTIQEDSAATSESLDVMASQKRPSQRHGSKYLATASTMDHARHGFLPRHRDTGILDSIGRFFGGDRGAPKRGSGKDSHHPARTAHYGSLPQKSHGRTQDENPVVHFFKNIVTPRTPPPSQGKGRGLSLSRFSWGAEGQRPGFGYGGRASDYKSAHKGFKGVDAQGTLSKIFKLGGRDSRSGSPMARR 4AD7305C1D5434C4 drb1_human HLA class II histocompatibility antigen, DRB1 beta chain HLA-DRB1 Human leukocyte antigen DRB1 protein protein human Homo sapiens Human MVCLKLPGGSCMTALTVTLMVLSSPLALSGDTRPRFLWQPKRECHFFNGTERVRFLDRYFYNQEESVRFDSDVGEFRAVTELGRPDAEYWNSQKDILEQARAAVDTYCRHNYGVVESFTVQRRVQPKVTVYPSKTQPLQHHNLLVCSVSGFYPGSIEVRWFLNGQEEKAGMVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVTSPLTVEWRARSESAQSKMLSGVGGFVLGLLFLGAGLFIYFRNQKGHSGLQPTGFLS 3B5912820A4654BE a0a1v1igj9_human HLA-DRB1 protein protein human Homo sapiens Human MVCLKFPGGSCMAALTVTLMVLSSPLALAGDTRPRFLEQVKHECHFFNGTERVRFLDRYFYHQEEYVRFDSDVGEYRAVTELGRPDAEYWNSQKDLLEQKRAAVDTYCRHNYGVGESFTVQRRVYPEVTVYPAKTQPLQHHNLLVCSVNGFYPGSIEVRWFRNGQEEKTGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSLTSPLTVEWRARSESAQSKMLSGVGGFVLGLLFLGAGLFIYFRNQKGHSGLQPTGFLS 8116E91DA38294E5 dma_human HLA class II histocompatibility antigen, DM alpha chain HLA-DMA DMA RING6 MHC class II antigen DMA Really interesting new gene 6 protein protein protein human Homo sapiens Human MGHEQNQGAALLQMLPLLWLLPHSWAVPEAPTPMWPDDLQNHTFLHTVYCQDGSPSVGLSEAYDEDQLFFFDFSQNTRVPRLPEFADWAQEQGDAPAILFDKEFCEWMIQQIGPKLDGKIPVSRGFPIAEVFTLKPLEFGKPNTLVCFVSNLFPPMLTVNWHDHSVPVEGFGPTFVSAVDGLSFQAFSYLNFTPEPSDIFSCIVTHEIDRYTAIAYWVPRNALPSDLLENVLCGVAFGLGVLGIIVGIVLIIYFRKPCSGD hg2a_human HLA class II histocompatibility antigen gamma chain DHLAG HLA-DR antigens-associated invariant chain Ia antigen-associated invariant chain CD74 protein protein human Homo sapiens Human MHRRRSRSCREDQKPVMDDQRDLISNNEQLPMLGRRPGAPESKCSRGALYTGFSILVTLLLAGQATTAYFLYQQQGRLDKLTVTSQNLQLENLRMKLPKPPKPVSKMRMATPLLMQALPMGALPQGPMQNATKYGNMTEDHVMHLLQNADPLKVYPPLKGSFPENLRHLKNTMETIDWKVFESWMHHWLLFEMSRHSLEQKPTDAPPKVLTKCQEEVSHIPAVHPGSFRPKCDENGNYLPLQCYGSIGYCWCVFPNGTEVPNTRSRGHHNCSESLELEDPSSGLGVTKQDLGPVPM 27A13F252D5FB91D dmb_human HLA class II histocompatibility antigen, DM beta chain HLA-DMB DMB RING7 MHC class II antigen DMB Really interesting new gene 7 protein protein protein human Homo sapiens Human MITFLPLLLGLSLGCTGAGGFVAHVESTCLLDDAGTPKDFTYCISFNKDLLTCWDPEENKMAPCEFGVLNSLANVLSQHLNQKDTLMQRLRNGLQNCATHTQPFWGSLTNRTRPPSVQVAKTTPFNTREPVMLACYVWGFYPAEVTITWRKNGKLVMPHSSAHKTAQPNGDWTYQTLSHLALTPSYGDTYTCVVEHIGAPEPILRDWTPGLSPMQTLKVSVSAVTLGLGLIIFSLGVISWRRAGHSSYTPLPGSNYSEGWHIS DIP protein P28068 original sequence version: 131 9B50F6CC22B3A4BA dra_human HLA class II histocompatibility antigen, DR alpha chain HLA-DRA MHC class II antigen DRA HLA-DRA1 protein protein human Homo sapiens Human MAISGVPVLGFFIIAVLMSAQESWAIKEEHVIIQAEFYLNPDQSGEFMFDFDGDEIFHVDMAKKETVWRLEEFGRFASFEAQGALANIAVDKANLEIMTKRSNYTPITNVPPEVTVLTNSPVELREPNVLICFIDKFTPPVVNVTWLRNGKPVTTGVSETVFLPREDHLFRKFHYLPFLPSTEDVYDCRVEHWGLDEPLLKHWEFDAPSPLPETTENVVCALGLTVGLVGIIIGTIFIIKGLRKSNAAERRGPL 3CD1CDBA89D92350 dma-hla_dmb 1 6 competitor competitor competitor neutral component neutral component region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P28067:p.Ile98Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain I A Wed May 02 17:05:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile98ala' Tue Jun 30 18:44:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile98Ala' P28067:p.[Ile98Ala;Pro194Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain P A Wed May 02 14:38:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile98ala,pro194ala' Tue Jun 30 17:14:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile98Ala;Pro194Ala]' spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 9 unspecified role unspecified role neutral component neutral component P01903:p.Gly53His mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain G H Wed May 02 16:07:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly53his' Tue Jun 30 18:29:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly53His' P01903:p.Gly53Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain G D Wed May 02 18:53:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly53asp' Tue Jun 30 20:06:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly53Asp' P01903:p.Phe49Ser mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain F S Wed May 02 18:06:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe49ser' Tue Jun 30 19:11:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe49Ser' crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 4 unspecified role unspecified role neutral component neutral component crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 3 unspecified role unspecified role neutral component neutral component region alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 competitor competitor competitor neutral component neutral component protein c tag tag tag range range range range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm association association Fig. 1A, 1C, 6B, 6D, 6E DRB1*04:01 was used in combination with DRA to assemble HLA-DR2, which then interacted with the MBP peptide. Interaction is disrupted by the presence of HLA-DM, which is made up of HLA-DMA and HLA-DMB. dma-hla_dmb-1 1 4 unspecified role unspecified role neutral component neutral component crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 9 unspecified role unspecified role neutral component neutral component P01903:p.Gly53His mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain G H Wed May 02 16:32:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly53his' Tue Jun 30 17:12:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly53His' P01903:p.Gly53Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain G D Wed May 02 16:30:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly53asp' Tue Jun 30 19:02:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly53Asp' P01903:p.Phe49Ser mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain F S Wed May 02 17:07:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe49ser' Tue Jun 30 16:43:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe49Ser' crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 3 unspecified role unspecified role neutral component neutral component region alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain association association Fig. 1A (black line) DRB1*04:01 was used in combination with DRA to assemble HLA-DR2, which then interacted with the MBP peptide. Interaction is disrupted by the presence of HLA-DM, which is made up of HLA-DMA and HLA-DMB. dma-hla_dmb-2 2 6 unspecified role unspecified role bait bait region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P28067:p.[Arg124Ala;Arg220Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A Fri Jul 22 10:52:50 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'arg220ala,arg124ala' Wed May 02 18:50:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg124ala,arg220ala' Tue Jun 30 18:30:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg124Ala;Arg220Ala]' P28067:p.Arg124Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R A Wed May 02 17:00:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg124ala' Tue Jun 30 19:11:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg124Ala' spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 9 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 3 competitor competitor competitor neutral component neutral component P02686:p.Pro89Val mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain P V Wed May 02 20:22:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro89val' Tue Jun 30 19:21:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro89Val' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P02686:p.Pro89Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain P D Wed May 02 18:01:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro89asp' Tue Jun 30 18:29:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro89Asp' P02686:p.Pro85Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain P D Wed May 02 18:22:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro85asp' Tue Jun 30 17:01:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro85Asp' P02686:p.Pro89Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain P A Wed May 02 16:12:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro89ala' Tue Jun 30 20:25:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro89Ala' spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 8 unspecified role unspecified role prey prey protein c tag tag tag range range range range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 7 unspecified role unspecified role prey prey region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster association association Fig. 1B, 1E, 2E, 2F CLIP 15mer is convalently attached via thrombin-cleavable linker at the n' of HLA-DRB injection of MBP peptide at stage 3 caused disassembly of the quaternary complex dma-hla_dmb-3 2 6 unspecified role unspecified role bait bait P28067:p.[Arg124Ala;Arg220Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A Wed May 02 14:37:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg124ala,arg220ala' Tue Jun 30 18:57:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg124Ala;Arg220Ala]' P28067:p.Arg124Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R A Wed May 02 18:56:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg124ala' Tue Jun 30 19:42:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg124Ala' region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 9 unspecified role unspecified role prey prey P01903:p.Phe49Ser mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain F S Wed May 02 19:14:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe49ser' Tue Jun 30 18:54:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe49Ser' P01903:p.Gly53His mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain G H Wed May 02 19:11:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly53his' Tue Jun 30 15:51:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly53His' P01903:p.Gly53Asp mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain G D Wed May 02 18:19:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly53asp' Tue Jun 30 19:08:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly53Asp' P01903:p.Gln43Phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain Q F Wed May 02 19:55:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln43phe' Tue Jun 30 17:52:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln43Phe' crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 8 unspecified role unspecified role prey prey protein c tag tag tag range range range range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm association association Fig. 1B, 1D, 6C, 6F, S2, S9 CLIP 15mer is convalently attached via thrombin-cleavable linker at the n' of HLA-DRB injection of MBP peptide at stage 3 cause disassembly of the quaternary complex dma-hla_dmb-4 2 6 unspecified role unspecified role bait bait P28067:p.[Arg124Ala;Arg220Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A Wed May 02 17:05:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg124ala,arg220ala' Tue Jun 30 16:59:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg124Ala;Arg220Ala]' P28067:p.Ile98Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain I A Wed May 02 15:59:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile98ala' Tue Jun 30 18:41:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile98Ala' region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 7 competitor competitor competitor ancillary ancillary region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 4 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 9 unspecified role unspecified role prey prey P01903:p.Trp68Phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain W F Wed May 02 19:35:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp68phe' Tue Jun 30 16:01:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp68Phe' crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 8 unspecified role unspecified role prey prey protein c tag tag tag range range range range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster association association original entry for mutations was:-pro106his [?-?] pro106asp [?-?] pro111lys [?-?] region [103-117] pro106lys [?-?] pro106phe [?-?] However dont know what the mutations refer to - so have altered to something non-specific. Fig. 1D, 2A, 2B, 2C, 2D, 6C, S9 CLIP 15mer is convalently attached via thrombin-cleavable linker at the n' of HLA-DRB injection of free CLIP peptide at stage 3 cause disassembly of the quaternary complex dma-hla_dmb-19 2 6 unspecified role unspecified role bait bait P28067:p.[Arg124Ala;Arg220Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A Wed May 02 18:45:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg124ala,arg220ala' Tue Jun 30 18:33:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg124Ala;Arg220Ala]' P28067:p.Arg124Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R A Wed May 02 17:39:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg124ala' Tue Jun 30 16:12:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg124Ala' region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 9 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 4 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 8 unspecified role unspecified role prey prey protein c tag tag tag range range range range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm association association Fig. 3A, S1 injection of CLIP peptide at stage 3 cause disassembly of the quaternary complex dma-hla_dmb-6 2 6 unspecified role unspecified role bait bait P28067:p.[Arg124Ala;Arg220Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A Wed May 02 20:24:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg124ala,arg220ala' Tue Jun 30 19:01:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg124Ala;Arg220Ala]' P28067:p.Ile98Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain I A Wed May 02 16:10:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile98ala' Tue Jun 30 18:23:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile98Ala' region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 7 competitor competitor competitor ancillary ancillary region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 8 unspecified role unspecified role prey prey protein c tag tag tag range range range range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 4 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 9 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster association association Fig. 3A injection of CLIP peptide at stage 3 cause disassembly of the quaternary complex dma-2b11-1 2 6 unspecified role unspecified role bait bait region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 7 unspecified role unspecified role prey prey P04233:p.[Met107Ala;Pro112_Leu113delinsGlyAla;Met115Ala] mutation mutation certain certain sequence position certain certain certain sequence position certain PL GA certain certain sequence position certain certain certain sequence position certain M A certain certain sequence position certain certain certain sequence position certain M A Thu Jul 07 12:17:03 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was met107ala,pro112gly,leu113ala,met115ala Fri Jul 22 10:52:33 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'met107ala,pro112gly,leu113ala,met115ala' Wed May 02 16:59:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met107ala,pro_leu112-113gly_ala,met115ala' Tue Jun 30 18:10:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Met107Ala;Pro112_Leu113delinsGlyAla;Met115Ala]' crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 4 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 8 unspecified role unspecified role prey prey protein c tag tag tag range range range range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 9 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster association association Fig. 3B, 3E, 3F, 4D, 4E, 4F, 5A, 5B, 7A, 7B, 7C, 7D, 7E, 7F, 7G, S8 Fig. 3E, 3F used HLA-DR preloaded with HA (306-318). Fig. 4D, 4E, 4F, 5A, 5B, 7B, 7D, 7E used HLA-DR linked with HA6 or HA3 peptides injection of HA peptide(306-318) at stage 3 cause disassembly of the quaternary complex dma-hla_dmb-8 2 6 unspecified role unspecified role bait bait region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 8 unspecified role unspecified role prey prey protein c tag tag tag range range range range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 4 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 9 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster association association Fig. 3C, S4, S7A, S7B HLA-DR2 (HLA-DRA :: HLA-DRB1) is preloaded with CLIP (103-117) peptide injection of free CLIP (87-101) or HA (306-318) peptide at stage 3 cause disassembly of the quaternary complex dma-hla_dmb-9 2 6 unspecified role unspecified role bait bait region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 7 competitor competitor competitor ancillary ancillary region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 9 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 8 unspecified role unspecified role prey prey protein c tag tag tag range range range range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 4 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster association association Fig. 3C, S7A HLA-DR2 (HLA-DRA :: HLA-DRB1) is preloaded with CLIP (103-117) peptide injection of free CLIP (87-101) or HA (306-318) peptide at stage 3 cause disassembly of the quaternary complex dma-hla_dmb-10 2 6 unspecified role unspecified role bait bait region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 9 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 4 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 3 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey protein c tag tag tag range range range range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm association association Fig. 3C HLA-DR2 (HLA-DRA :: HLA-DRB1) is preloaded with MBP peptide injection of free CLIP (87-101) peptide at stage 3 cause disassembly of the quaternary complex dma-hla_dmb-11 2 6 unspecified role unspecified role bait bait region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 7 competitor competitor competitor ancillary ancillary region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 3 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 9 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 8 unspecified role unspecified role prey prey protein c tag tag tag range range range range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm association association Fig. 3C HLA-DR2 (HLA-DRA :: HLA-DRB1) is preloaded with MBP peptide injection of free CLIP (87-101) peptide at stage 3 cause disassembly of the quaternary complex dma-hla_dmb-12 2 6 unspecified role unspecified role bait bait region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 3 competitor competitor competitor ancillary ancillary region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 3 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 8 unspecified role unspecified role prey prey protein c tag tag tag range range range range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm association association Fig. 3D HLA-DR2 (HLA-DRA :: HLA-DRB1) is preloaded with MBP peptide injection of free MBP(85-99) peptide at stage 3 cause disassembly of the quaternary complex dma-hla_dmb-13 2 6 unspecified role unspecified role bait bait region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 4 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 8 unspecified role unspecified role prey prey protein c tag tag tag range range range range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 9 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster association association Fig. 3E injection of HA peptide(306-318) at stage 3 cause disassembly of the quaternary complex dma-2b1f 2 6 unspecified role unspecified role bait bait region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 4 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 9 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 8 unspecified role unspecified role prey prey protein c tag tag range range range range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm association association Fig. 3G injection of HA peptide(306-318) at stage 3 cause disassembly of the quaternary complex HLA-DR4 (HLA-DRA :: HLA-DRB4) was preloaded with HA(306-318) peptide dma-hla_dmb-15 2 6 unspecified role unspecified role bait bait region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 9 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 8 unspecified role unspecified role prey prey protein c tag tag range range range range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 4 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster association association Fig. 3G HLA-DR4 (HLA-DRA :: HLA-DRB4) was preloaded with CLIP peptide injection of free HA peptide(306-318) at stage 3 cause disassembly of the quaternary complex dma-hla_dmb-16 2 6 unspecified role unspecified role bait bait P28067:p.[Arg124Ala;Arg220Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A Wed May 02 17:35:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg124ala,arg220ala' Tue Jun 30 16:55:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg124Ala;Arg220Ala]' P28067:p.Arg124Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R A Wed May 02 20:12:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg124ala' Tue Jun 30 18:24:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg124Ala' region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain dnp tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 9 unspecified role unspecified role prey prey P01903:p.Gly74Ser mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain G S Wed May 02 20:20:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly74ser' Tue Jun 30 16:11:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly74Ser' P01903:p.Ser78His mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain S H Wed May 02 16:39:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser78his' Tue Jun 30 16:27:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser78His' P01903:p.Trp68Phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain W F Wed May 02 18:21:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp68phe' Tue Jun 30 18:44:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp68Phe' crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 8 unspecified role unspecified role prey prey protein c tag tag tag range range range range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm association association Fig. S3 DNP-CLIP 15mer is convalently attached via thrombin-cleavable linker at the n' of HLA-DRB injection of MBP peptide at stage 3 cause disassembly of the quaternary complex dma-hla_dmb-18 2 6 unspecified role unspecified role bait bait region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 3 competitor competitor competitor ancillary ancillary region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 8 unspecified role unspecified role prey prey protein c tag tag tag range range range range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 4 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster association association Fig. S4 HLA-DR2 (HLA-DRA :: HLA-DRB1) is preloaded with CLIP (103-117) peptide injection of free MBP peptide at stage 3 cause disassembly of the quaternary complex