UniProt UniProt http://www.uniprot.org Two interacting coiled-coil proteins, WEB1 and PMI2, maintain the chloroplast photorelocation movement velocity in Arabidopsis. Two interacting coiled-coil proteins, WEB1 and PMI2, maintain the chloroplast photorelocation movement velocity in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2010 imex curation Kodama Y., Suetsugu N., Kong S.G., Wada M. wadascb@kyushu-u.org 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions arath-protoplast Arabidopsis thaliana protoplast Protoplast defined as a membrane-bound cell from which the outer wall has been partially or completely removed. fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Kodama Y., Suetsugu N., Kong S.G., Wada M. wadascb@kyushu-u.org Accepted 2011-SEP-23 by ORCHARD Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2010 Only protein-protein interactions imex curation Two interacting coiled-coil proteins, WEB1 and PMI2, maintain the chloroplast photorelocation movement velocity in Arabidopsis. Two interacting coiled-coil proteins, WEB1 and PMI2, maintain the chloroplast photorelocation movement velocity in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2010 imex curation Kodama Y., Suetsugu N., Kong S.G., Wada M. wadascb@kyushu-u.org 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions arath-protoplast Arabidopsis thaliana protoplast Protoplast defined as a membrane-bound cell from which the outer wall has been partially or completely removed. bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Kodama Y., Suetsugu N., Kong S.G., Wada M. wadascb@kyushu-u.org Accepted 2011-SEP-23 by ORCHARD 21-Dec-2011: Contacted by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2010 Only protein-protein interactions imex curation Two interacting coiled-coil proteins, WEB1 and PMI2, maintain the chloroplast photorelocation movement velocity in Arabidopsis. Two interacting coiled-coil proteins, WEB1 and PMI2, maintain the chloroplast photorelocation movement velocity in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2010 imex curation Kodama Y., Suetsugu N., Kong S.G., Wada M. wadascb@kyushu-u.org 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kodama Y., Suetsugu N., Kong S.G., Wada M. wadascb@kyushu-u.org Accepted 2011-SEP-23 by ORCHARD 21-Dec-2011: Contacted by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2010 Only protein-protein interactions imex curation Two interacting coiled-coil proteins, WEB1 and PMI2, maintain the chloroplast photorelocation movement velocity in Arabidopsis. Two interacting coiled-coil proteins, WEB1 and PMI2, maintain the chloroplast photorelocation movement velocity in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2010 imex curation Kodama Y., Suetsugu N., Kong S.G., Wada M. wadascb@kyushu-u.org 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions allce Allium cepa (Onion) Onion fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Kodama Y., Suetsugu N., Kong S.G., Wada M. wadascb@kyushu-u.org Accepted 2011-SEP-23 by ORCHARD 21-Dec-2011: Contacted by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2010 Only protein-protein interactions imex curation Two interacting coiled-coil proteins, WEB1 and PMI2, maintain the chloroplast photorelocation movement velocity in Arabidopsis. Two interacting coiled-coil proteins, WEB1 and PMI2, maintain the chloroplast photorelocation movement velocity in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2010 imex curation Kodama Y., Suetsugu N., Kong S.G., Wada M. wadascb@kyushu-u.org 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions allce Allium cepa (Onion) Onion bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Kodama Y., Suetsugu N., Kong S.G., Wada M. wadascb@kyushu-u.org Accepted 2011-SEP-23 by ORCHARD 21-Dec-2011: Contacted by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2010 Only protein-protein interactions imex curation pmi2_arath Protein PLASTID MOVEMENT IMPAIRED 2 PMI2 F4N21.22 At1g66840 WEB2 Protein WEAK CHLOROPLAST MOVEMENT UNDER BLUE LIGHT 2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGERNLDGTVSVKATINKYGQKATRSVIKSSVAEDLHKSGRELGIYRESRRVAESAKAKAEVELCKAKKIVKELTLRIEESNRRLKSRRIDIEAVMNESRIDGNGGYVRIMRELEDMKQELSKLKLDVVYVSREKVVAEKEVMELESRMEENLKLLESLKLEVDVANEEHVLVEVAKIEALKECKEVEEQREKERKEVSESLHKRKKRIREMIREIERSKNFENELAETLLDIEMLETQLKLVKEMERKVQRNESMSRSKNRAFERGKDNLSVLKEVTEATEAKKAELASINAELFCLVNTMDTLRKEFDHAKKETAWLDKMIQKDDVMLERLNTKLLIAKDQLEAVSKAEERISYLADNLTTSFEKLKSDREAAKKEELKLREEARIINNEIQKTETGFDGKEKELLSKLDELEKAKHAESLALEKLETMVEKTMETREMESRRNSTITISRFEYEYLSGKACHAEETAEKKVEAAMAWVEALKASTKAIMIKTESLKRVSGKTMLEEERESFRMQRSLSIKRLVQDEIQKFKGNSEDNGLINSPKPVRKSVRLSGKFAPVQGGKSRRYSSGNRATPTFFVIKKKKKVPNMVKFFSRKRRNSSLEQ 3BCC9D4188A87158 web1_arath Protein WEAK CHLOROPLAST MOVEMENT UNDER BLUE LIGHT 1 At2g26570 WEB1 T9J22.24 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEDLKTVEASDNVVSDNVEKVNPELIDSTIRESNIQSATKVDNIPQSQTDTEETQQSQTDTEETQQSQTDDTTGNAKIYVDDTFSPSDAATAAVLTGKDSTSTTIVEEVMEPDEIGLPSVKITEAATGTARNGGGSPRTVSSPRFSGSPVSTGTPKNVDSHRGLIDTAAPFESVKEAVSKFGGITDWKSHRMQAVERRKLIEEELKKIHEEIPEYKTHSETAEAAKLQVLKELESTKRLIEQLKLNLDKAQTEEQQAKQDSELAKLRVEEMEQGIAEDVSVAAKAQLEVAKARHTTAITELSSVKEELETLHKEYDALVQDKDVAVKKVEEAMLASKEVEKTVEELTIELIATKESLESAHASHLEAEEQRIGAAMARDQDTHRWEKELKQAEEELQRLNQQIHSSKDLKSKLDTASALLLDLKAELVAYMESKLKQEACDSTTNTDPSTENMSHPDLHAAVASAKKELEEVNVNIEKAAAEVSCLKLASSSLQLELEKEKSTLASIKQREGMASIAVASIEAEIDRTRSEIASVQSKEKDAREKMVELPKQLQQAAEEADEAKSLAEVAREELRKAKEEAEQAKAGASTMESRLFAAQKEIEAAKASERLALAAIKALEESESTLKANDTDSPRSVTLSLEEYYELSKRAHEAEELANARVAAAVSRIEEAKETEMRSLEKLEEVNRDMDARKKALKEATEKAEKAKEGKLGVEQELRKWRAEHEQKRKAGDGVNTEKNLKESFEGGKMEQSPEAVVYASSPSESYGTEENSETNLSPQTKSRKKKKKLSFPRFFMFLSKKKSHNN F83F0655E1371472 o48724-pmi2 1 7 unspecified role unspecified role neutral component neutral component region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig. 1I pmi2-o48724 3 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Fig. 2A, S6B o48724-pmi2-1 3 7 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association Fig. 2A, S6B o48724-pmi2-2 4 7 unspecified role unspecified role neutral component neutral component region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig. 2B o48724-pmi2-3 2 6 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 2C o48724-o48724 2 7 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 2D, Supp. Fig. 4 o48724-pmi2-4 5 7 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. S4A