MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Protein phosphatase 2A negatively regulates eukaryotic initiation factor 4E phosphorylation and eIF4F assembly through direct dephosphorylation of Mnk and eIF4E. Protein phosphatase 2A negatively regulates eukaryotic initiation factor 4E phosphorylation and eIF4F assembly through direct dephosphorylation of Mnk and eIF4E. Neoplasia (1522-8002) 2010 rapid curation Li Y., Yue P., Deng X., Ueda T., Fukunaga R., Khuri FR., Sun SY. suggested by lperfetto 30-Sept-2013: Contacted by IntAct-Help. partial coverage rapid curation in vitro In vitro phosphatase assay phosphatase assay predetermined predetermined participant predetermined Li Y., Yue P., Deng X., Ueda T., Fukunaga R., Khuri FR., Sun SY. suggested by lperfetto Accepted 2013-SEP-25 by MARTA 30-Sept-2013: Contacted by IntAct-Help. Neoplasia (1522-8002) 2010 partial coverage rapid curation rapid curation Protein phosphatase 2A negatively regulates eukaryotic initiation factor 4E phosphorylation and eIF4F assembly through direct dephosphorylation of Mnk and eIF4E. Protein phosphatase 2A negatively regulates eukaryotic initiation factor 4E phosphorylation and eIF4F assembly through direct dephosphorylation of Mnk and eIF4E. Neoplasia (1522-8002) 2010 rapid curation Li Y., Yue P., Deng X., Ueda T., Fukunaga R., Khuri FR., Sun SY. suggested by lperfetto 30-Sept-2013: Contacted by IntAct-Help. partial coverage rapid curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Li Y., Yue P., Deng X., Ueda T., Fukunaga R., Khuri FR., Sun SY. suggested by lperfetto Accepted 2013-SEP-25 by MARTA 30-Sept-2013: Contacted by IntAct-Help. Neoplasia (1522-8002) 2010 partial coverage rapid curation rapid curation mknk1_human MAP kinase-interacting serine/threonine-protein kinase 1 MAP kinase signal-integrating kinase 1 MKNK1 MNK1 protein protein human Homo sapiens Human MVSSQKLEKPIEMGSSEPLPIADGDRRRKKKRRGRATDSLPGKFEDMYKLTSELLGEGAYAKVQGAVSLQNGKEYAVKIIEKQAGHSRSRVFREVETLYQCQGNKNILELIEFFEDDTRFYLVFEKLQGGSILAHIQKQKHFNEREASRVVRDVAAALDFLHTKDKVSLCHLGWSAMAPSGLTAAPTSLGSSDPPTSASQVAGTTGIAHRDLKPENILCESPEKVSPVKICDFDLGSGMKLNNSCTPITTPELTTPCGSAEYMAPEVVEVFTDQATFYDKRCDLWSLGVVLYIMLSGYPPFVGHCGADCGWDRGEVCRVCQNKLFESIQEGKYEFPDKDWAHISSEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPEKGLPTPQVLQRNSSTMDLTLFAAEAIALNRQLSQHEENELAEEPEALADGLCSMKLSPPCKSRLARRRALAQAGRGEDRSPPTAL CAE225C35DCB2B43 if4e_human Eukaryotic translation initiation factor 4E mRNA cap-binding protein eIF-4F 25 kDa subunit EIF4E EIF4EL1 EIF4F protein protein human Homo sapiens Human MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV DIP protein P06730 original sequence version: 164 DIP protein P06730 original sequence version: 127 DIP protein P06730 original sequence version: 126 DIP protein P06730 original sequence version: 135 DIP protein P06730 original sequence version: 151 DIP protein P06730 original sequence version: 155 DIP protein P06730 original sequence version: 160 DIP protein P06730-1 original sequence version: 187 DIP protein P06730 original sequence version: 146 B869B8DE615E699D q9bub5-3 MAP kinase-interacting serine/threonine-protein kinase 1 MAP kinase signal-integrating kinase 1 MKNK1 MNK1 MNK1b protein protein human Homo sapiens Human MVSSQKLEKPIEMGSSEPLPIADGDRRRKKKRRGRATDSLPGKFEDMYKLTSELLGEGAYAKVQGAVSLQNGKEYAVKIIEKQAGHSRSRVFREVETLYQCQGNKNILELIEFFEDDTRFYLVFEKLQGGSILAHIQKQKHFNEREASRVVRDVAAALDFLHTKGIAHRDLKPENILCESPEKVSPVKICDFDLGSGMKLNNSCTPITTPELTTPCGSAEYMAPEVVEVFTDQATFYDKRCDLWSLGVVLYIMLSGYPPFVGHCGADCGWDRGEVCRVCQNKLFESIQEGKYEFPDKDWAHISSEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGEQQHNGPDALRS 1CCA03B12D39641F pp2aa_human Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform PPP2CA Replication protein C protein protein human Homo sapiens Human MDEKVFTKELDQWIEQLNECKQLSESQVKSLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFRIGGKSPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYRERITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDYLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHVTRRTPDYFL DIP protein P67775 original sequence version: 112 DIP protein P67775-1 original sequence version: 98 DIP protein P67775 original sequence version: 29 DIP protein P67775 original sequence version: 102 DIP protein P67775 original sequence version: 65 DIP protein P67775 original sequence version: 67 DIP protein P67775-1 original sequence version: 114 DIP protein P67775 original sequence version: 113 DIP protein P67775-1 original sequence version: 122 C602291F78F34555 eif4e-ppp2ca-3 2 6 unspecified role unspecified role bait bait under-expressed under expressed level under-expressed 4 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Resulting Phenotypic qualities (properties)[PATO]: PATO:0002221 (increased phosphorylation) physical association physical association f3b homomint mint mknk1-ppp2ca-1 2 5 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined prerequisite-ptm Resulting Phenotypic qualities (properties)[PATO]: PATO:0002221 (increased phosphorylation) 6 enzyme target enzyme target substrate substrate prey prey under-expressed under expressed level under-expressed physical association physical association f3a homomint mint eif4e-ppp2ca-2 1 6 enzyme enzyme neutral component neutral component over-expressed over expressed level over-expressed 4 enzyme target enzyme target substrate substrate neutral component neutral component phosphorylated phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) certain certain sequence position certain certain certain sequence position certain prerequisite-ptm Resulting Phenotypic qualities (properties)[PATO]: PATO:0002221 (increased phosphorylation) physical association physical association f3b homomint mint mknk1-ppp2ca-2 1 3 enzyme target enzyme target substrate substrate neutral component neutral component phosphorylated phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) certain certain sequence position certain certain certain sequence position certain prerequisite-ptm phosphorylated phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) certain certain sequence position certain certain certain sequence position certain prerequisite-ptm Resulting Phenotypic qualities (properties)[PATO]: PATO:0002221 (increased phosphorylation) 6 enzyme enzyme neutral component neutral component over-expressed over expressed level over-expressed physical association physical association f3a homomint mint