UniProt UniProt http://www.uniprot.org Factor X/Xa elicits protective signaling responses in endothelial cells directly via PAR-2 and indirectly via endothelial protein C receptor-dependent recruitment of PAR-1. Factor X/Xa elicits protective signaling responses in endothelial cells directly via PAR-2 and indirectly via endothelial protein C receptor-dependent recruitment of PAR-1. J. Biol. Chem. (0021-9258) 2010 imex curation Bae J.S., Yang L., Rezaie A.R. rezaiear@slu.edu 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions human-ea.hy926 Homo sapiens EA.hy926 ea.hy926 EA.hy926 http://www.lgcstandards-atcc.org/LGCAdvancedCatalogueSearch/ProductDescription/tabid/1068/Default.aspx?ATCCNum=CRL-2922&Template=cellBiology This cell line was established by fusing primary human umbilical vein cells (HUVEC) with a thioguanine-resistant clone of A549 by exposure to polyethylene glycol (PEG). anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bae J.S., Yang L., Rezaie A.R. rezaier@slu.edu rezaiear@slu.edu Accepted 2011-SEP-14 by ORCHARD J. Biol. Chem. (0021-9258) 2010 Only protein-protein interactions imex curation Factor X/Xa elicits protective signaling responses in endothelial cells directly via PAR-2 and indirectly via endothelial protein C receptor-dependent recruitment of PAR-1. Factor X/Xa elicits protective signaling responses in endothelial cells directly via PAR-2 and indirectly via endothelial protein C receptor-dependent recruitment of PAR-1. J. Biol. Chem. (0021-9258) 2010 imex curation Bae J.S., Yang L., Rezaie A.R. rezaiear@slu.edu 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions human-uvec Human Umbilical Vein Endothelial Cells (HUVEC) huvec umbilical_vein_endothelium Umbilical vein endothelial cell [Umbilical vein endothelium] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bae J.S., Yang L., Rezaie A.R. rezaier@slu.edu rezaiear@slu.edu Accepted 2011-SEP-14 by ORCHARD 21-Dec-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2010 Only protein-protein interactions imex curation par2_human Proteinase-activated receptor 2 F2RL1 GPR11 PAR2 Coagulation factor II receptor-like 1 G-protein coupled receptor 11 Thrombin receptor-like 1 protein protein human Homo sapiens Human MRSPSAAWLLGAAILLAASLSCSGTIQGTNRSSKGRSLIGKVDGTSHVTGKGVTVETVFSVDEFSASVLTGKLTTVFLPIVYTIVFVVGLPSNGMALWVFLFRTKKKHPAVIYMANLALADLLSVIWFPLKIAYHIHGNNWIYGEALCNVLIGFFYGNMYCSILFMTCLSVQRYWVIVNPMGHSRKKANIAIGISLAIWLLILLVTIPLYVVKQTIFIPALNITTCHDVLPEQLLVGDMFNYFLSLAIGVFLFPAFLTASAYVLMIRMLRSSAMDENSEKKRKRAIKLIVTVLAMYLICFTPSNLLLVVHYFLIKSQGQSHVYALYIVALCLSTLNSCIDPFVYYFVSHDFRDHAKNALLCRSVRTVKQMQVSLTSKKHSRKSSSYSSSSTTVKTSY DIP protein P55085 original sequence version: 142 F1A4E1D5AB9B362B q14868_human EPR-1 protein protein human Homo sapiens Human MTSRGFQRSCNNPPCSSMTGRRANQIHHLTPDFSLRELLPPKKAGTWADCVSPPCGERDRCEGWADRHTACSSPASTCQVHTQDCDSLNNMRSRHIHCGRLCHANKAVSSSKRDTAFFLPHFSPGKPGNQNSKNEPPKKRERERSSHCYPAAPAAQAEAPLVPLSRQNKSTVETSNLKMLISFPKTLLRGPQEGWWHQGINPGSGAATLGPGSSERPQSIEASCSMARRTFFAVSSNSFFLLLVSFAILFLALSLSSFKNSPRVNSSNCFLTERKAQPDECFLCSSMGSSSGSQPSSSLKQKKHWAKSGSFSVGQWMKPASAIRSGVQRSPPRRASS 72B5AF24E8CBC09F par1_human Proteinase-activated receptor 1 CF2R PAR1 TR Coagulation factor II receptor Thrombin receptor F2R protein protein human Homo sapiens Human MGPRRLLLVAACFSLCGPLLSARTRARRPESKATNATLDPRSFLLRNPNDKYEPFWEDEEKNESGLTEYRLVSINKSSPLQKQLPAFISEDASGYLTSSWLTLFVPSVYTGVFVVSLPLNIMAIVVFILKMKVKKPAVVYMLHLATADVLFVSVLPFKISYYFSGSDWQFGSELCRFVTAAFYCNMYASILLMTVISIDRFLAVVYPMQSLSWRTLGRASFTCLAIWALAIAGVVPLLLKEQTIQVPGLNITTCHDVLNETLLEGYYAYYFSAFSAVFFFVPLIISTVCYVSIIRCLSSSAVANRSKKSRALFLSAAVFCIFIICFGPTNVLLIAHYSFLSHTSTTEAAYFAYLLCVCVSSISCCIDPLIYYYASSECQRYVYSILCCKESSDPSSYNSSGQLMASKMDTCSSNLNNSIYKKLLT DIP protein P25116 original sequence version: 159 DIP protein P25116 original sequence version: 161 DIP protein P25116 original sequence version: 93 41B742A99EEC96AB epcr_human Endothelial protein C receptor Endothelial cell protein C receptor Activated protein C receptor PROCR EPCR protein protein human Homo sapiens Human MLTTLLPILLLSGWAFCSQDASDGLQRLHMLQISYFRDPYHVWYQGNASLGGHLTHVLEGPDTNTTIIQLQPLQEPESWARTQSGLQSYLLQFHGLVRLVHQERTLAFPLTIRCFLGCELPPEGSRAHVFFEVAVNGSSFVSFRPERALWQADTQVTSGVVTFTLQQLNAYNRTRYELREFLEDTCVQYVQKHISAENTKGSQTSRSYTSLVLGVLVGSFIIAGVAVGIFLCTGGRRC DIP protein Q9UNN8 original sequence version: 130 DIP protein Q9UNN8 original sequence version: 97 EA7F0658CD19695C cav1_human Caveolin-1 CAV1 CAV protein protein human Homo sapiens Human MSGGKYVDSEGHLYTVPIREQGNIYKPNNKAMADELSEKQVYDAHTKEIDLVNRDPKHLNDDVVKIDFEDVIAEPEGTHSFDGIWKASFTTFTVTKYWFYRLLSALFGIPMALIWGIYFAILSFLHIWAVVPCIKSFLIEIQCISRVYSIYVHTVCDPLFEAVGKIFSNVRINLQKEI DIP protein Q03135 original sequence version: 164 DIP protein Q03135 original sequence version: 119 DIP protein Q03135 original sequence version: 122 DIP protein Q03135-1 original sequence version: 122 DIP protein Q03135 original sequence version: 133 DIP protein Q03135 original sequence version: 155 DIP protein Q03135 original sequence version: 147 2424DE9B5E6521D5 f2r-cav1 1 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait association association 5a cav1-f2r 1 7 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey association association 5b procr-cav1 1 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait association association 5c epr1-f2rl1 2 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association 6c f2rl1-epr1 2 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association 6c epr1-f2rl1-1 1 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association 6c f2rl1-epr1-1 1 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association 6c procr-cav1-1 1 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association 5c When endothelial cells were pretreated with Factor FX (Ser195Ala), EPCR and caveolin-1 were not co-immunoprecipitated. cav1-f2r-1 1 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait association association 5b When endothelial cells were pretreated with Factor FX (Ser195Ala), EPCR and caveolin-1 were not co-immunoprecipitated.