I2D I2D Direct interaction between NHERF1 and Frizzled regulates ?-catenin signaling. Direct interaction between NHERF1 and Frizzled regulates ?-catenin signaling. Oncogene (0950-9232) 2011 imex curation Wheeler DS., Barrick SR., Grubisha MJ., Brufsky AM., Friedman PA., Romero G. Cancer - Interactions investigated in the context of cancer Author confirms that NHERF1 was of human origin. ggr@pitt.edu jyoti Only protein-protein interactions Curated by: I2D - Interologous Interaction Database in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting anti tag immunost anti tag immunostaining anti tag immunost Wheeler DS., Barrick SR., Grubisha MJ., Brufsky AM., Friedman PA., Romero G. Cancer - Interactions investigated in the context of cancer Author confirms that NHERF1 was of human origin. ggr@pitt.edu Accepted 2011-MAR-30 by JYOTI Oncogene (0950-9232) 2011 jyoti Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Direct interaction between NHERF1 and Frizzled regulates ?-catenin signaling. Direct interaction between NHERF1 and Frizzled regulates ?-catenin signaling. Oncogene (0950-9232) 2011 imex curation Wheeler DS., Barrick SR., Grubisha MJ., Brufsky AM., Friedman PA., Romero G. Cancer - Interactions investigated in the context of cancer Author confirms that NHERF1 was of human origin. ggr@pitt.edu jyoti Only protein-protein interactions Curated by: I2D - Interologous Interaction Database crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wheeler DS., Barrick SR., Grubisha MJ., Brufsky AM., Friedman PA., Romero G. Cancer - Interactions investigated in the context of cancer Author confirms that NHERF1 was of human origin. Author confirms that NHERF1 was of human origin. ggr@pitt.edu Accepted 2011-MAR-30 by JYOTI Oncogene (0950-9232) 2011 jyoti Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Direct interaction between NHERF1 and Frizzled regulates ?-catenin signaling. Direct interaction between NHERF1 and Frizzled regulates ?-catenin signaling. Oncogene (0950-9232) 2011 imex curation Wheeler DS., Barrick SR., Grubisha MJ., Brufsky AM., Friedman PA., Romero G. Cancer - Interactions investigated in the context of cancer Author confirms that NHERF1 was of human origin. ggr@pitt.edu jyoti Only protein-protein interactions Curated by: I2D - Interologous Interaction Database crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wheeler DS., Barrick SR., Grubisha MJ., Brufsky AM., Friedman PA., Romero G. Cancer - Interactions investigated in the context of cancer Author confirms that NHERF1 was of human origin. ggr@pitt.edu Accepted 2011-MAR-30 by JYOTI Oncogene (0950-9232) 2011 jyoti Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database g3gth2_crigr Na(+)/H(+) exchange regulatory cofactor NHE-RF I79_000956 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MSADAAAGEPLPRLCCLEKGPNGYGFHLHGEKGKVGQFIRLVEPGSPAEKSGLLAGDRLVEVNGENVEKETHQQVVSRIRGALNAVRLLVVDPETDEHLKKLGVPIREDLLRSQKSEQAEPPAAADAQATDQNEAEKSHLERRELRPRLCTMKKGPNGYGFNLHSDKSKPGQFIRAVDPDSPAEASGLRAQDRIVEVNGVCMEGKQHGDVVSAIKAGGDEAKLLVVDKETDEFFKKCKVIPSQEHLDGPLPEPFTNGEIQKESSREALVEAASESPRPALVRSASSDTSEELNSQDSPKRQESTEPSSTSSSSDPILDFNISLAVAKERAHQKRSSKRAPQMDWSKKNELFSNL 30F68A569E253F91 dvl2_mouse Segment polarity protein dishevelled homolog DVL-2 Dvl2 DSH homolog 2 protein protein mouse Mus musculus Mouse MAGSSAGGGGVGETKVIYHLDEEETPYLVKIPVPAERITLGDFKSVLQRPAGAKYFFKSMDQDFGVVKEEISDDNARLPCFNGRVVSWLVSSDTPQPEVAPPAHESRTELVPPPPPLPPLPPERTSGIGDSRPPSFHPNVSSSHENLEPETETESVVSLRRDRPRRRDSSEHGAGGHRPGGPSRLERHLAGYESSSTLMTSELESTSLGDSDEDDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRMERTSSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDMNFENMSNDDAVRVLRDIVHKPGPIVLTVAKCWDPSPQAYFTLPRNEPIQPIDPAAWVSHSAALTGAFPAYPGSSSMSTITSGSSLPDGCEGRGLSVHMDMASVTKAMAAPESGLEVRDRMWLKITIPNAFLGSDVVDWLYHHVEGFPERREARKYASGLLKAGLIRHTVNKITFSEQCYYVFGDLSGGCESYLVNLSLNDNDGSSGASDQDTLAPLPGATPWPLLPTFSYQYPAPHPYSPQPPPYHELSSYTYGGGSASSQHSEGSRSSGSTRSDGGAGRTGRPEERAPESKSGSGSESELSSRGGSLRRGGEPGGTGDGGPPPSRGSTGAPPNLRALPGLHPYGAPSGMALPYNPMMVVMMPPPPPPVSTAVQPPGAPPVRDLGSVPPELTASRQSFHMAMGNPSEFFVDVM DIP protein Q60838 original sequence version: 132 DIP protein Q60838 original sequence version: 63 DIP protein Q60838 original sequence version: 125 DIP protein Q60838 original sequence version: 69 DIP protein Q60838 original sequence version: 91 928A527143BBBBB0 fzd4_human Frizzled-4 FZD4 FzE4 protein protein human Homo sapiens Human MAWRGAGPSVPGAPGGVGLSLGLLLQLLLLLGPARGFGDEEERRCDPIRISMCQNLGYNVTKMPNLVGHELQTDAELQLTTFTPLIQYGCSSQLQFFLCSVYVPMCTEKINIPIGPCGGMCLSVKRRCEPVLKEFGFAWPESLNCSKFPPQNDHNHMCMEGPGDEEVPLPHKTPIQPGEECHSVGTNSDQYIWVKRSLNCVLKCGYDAGLYSRSAKEFTDIWMAVWASLCFISTAFTVLTFLIDSSRFSYPERPIIFLSMCYNIYSIAYIVRLTVGRERISCDFEEAAEPVLIQEGLKNTGCAIIFLLMYFFGMASSIWWVILTLTWFLAAGLKWGHEAIEMHSSYFHIAAWAIPAVKTIVILIMRLVDADELTGLCYVGNQNLDALTGFVVAPLFTYLVIGTLFIAAGLVALFKIRSNLQKDGTKTDKLERLMVKIGVFSVLYTVPATCVIACYFYEISNWALFRYSADDSNMAVEMLKIFMSLLVGITSGMWIWSAKTLHTWQKCSNRLVNSGKVKREKRGNGWVKPGKGSETVV E0A83ECEC560A381 nhrf1_human Na(+)/H(+) exchange regulatory cofactor NHE-RF1 SLC9A3R1 NHERF NHERF1 Ezrin-radixin-moesin-binding phosphoprotein 50 Regulatory cofactor of Na(+)/H(+) exchanger Sodium-hydrogen exchanger regulatory factor 1 Solute carrier family 9 isoform A3 regulatory factor 1 protein protein human Homo sapiens Human MSADAAAGAPLPRLCCLEKGPNGYGFHLHGEKGKLGQYIRLVEPGSPAEKAGLLAGDRLVEVNGENVEKETHQQVVSRIRAALNAVRLLVVDPETDEQLQKLGVQVREELLRAQEAPGQAEPPAAAEVQGAGNENEPREADKSHPEQRELRPRLCTMKKGPSGYGFNLHSDKSKPGQFIRSVDPDSPAEASGLRAQDRIVEVNGVCMEGKQHGDVVSAIRAGGDETKLLVVDRETDEFFKKCRVIPSQEHLNGPLPVPFTNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL E33AF87016D37A65 nherf1-fzd4 1 6 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9ULV1:p.Val537Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain V A Wed May 02 19:55:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val537ala' Tue Jun 30 17:58:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val537Ala' crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 7 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1d nherf1-fzd4-1 3 6 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9ULV1:p.Val537Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain V A Wed May 02 19:13:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val537ala' Tue Jun 30 18:16:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val537Ala' crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 4 unspecified role unspecified role prey prey physical association physical association Fig. 1b nherf1-fzd4-2 2 6 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 4 unspecified role unspecified role prey prey G3GTH2:p.[His164Ala;Lys167Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain H A Fri Jul 22 11:09:34 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'lys167ala,his164ala' Wed May 02 19:23:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his164ala,lys167ala' Tue Jun 30 16:05:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[His164Ala;Lys167Ala]' G3GTH2:p.[Tyr24Ala;Phe26Ala;His164Ala;Lys167Ala] tyr24ala-phe26ala-tyr164ala-phe167ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain K A Wed May 02 16:25:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr24ala,phe26ala,his164ala,lys167ala' Tue Jun 30 18:31:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr24Ala;Phe26Ala;His164Ala;Lys167Ala]' physical association physical association Fig. 1c, S3 fzd4-dvl2 3 5 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9ULV1:p.Val537Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain V A Wed May 02 15:01:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val537ala' Tue Jun 30 18:49:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val537Ala' crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association Fig. 6a