IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Dyslexia-associated kiaa0319-like protein interacts with axon guidance receptor nogo receptor 1. Dyslexia-associated kiaa0319-like protein interacts with axon guidance receptor nogo receptor 1. Cell. Mol. Neurobiol. (0272-4340) 2011 imex curation Poon M.W., Tsang W.H., Chan S.O., Li H.M., Ng H.K., Waye M.M. Positive control interaction could not be added as the species of the proteins could not be traced. mary-waye@cuhk.edu.hk 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Poon M.W., Tsang W.H., Chan S.O., Li H.M., Ng H.K., Waye M.M. Positive control interaction could not be added as the species of the proteins could not be traced. mary-waye@cuhk.edu.hk Accepted 2011-NOV-10 by MGT 21-Dec-2011: Contacted by JYOTI. Cell. Mol. Neurobiol. (0272-4340) 2011 Only protein-protein interactions imex curation Dyslexia-associated kiaa0319-like protein interacts with axon guidance receptor nogo receptor 1. Dyslexia-associated kiaa0319-like protein interacts with axon guidance receptor nogo receptor 1. Cell. Mol. Neurobiol. (0272-4340) 2011 imex curation Poon M.W., Tsang W.H., Chan S.O., Li H.M., Ng H.K., Waye M.M. Positive control interaction could not be added as the species of the proteins could not be traced. mary-waye@cuhk.edu.hk 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Poon M.W., Tsang W.H., Chan S.O., Li H.M., Ng H.K., Waye M.M. Positive control interaction could not be added as the species of the proteins could not be traced. mary-waye@cuhk.edu.hk Accepted 2011-NOV-10 by MGT 21-Dec-2011: Contacted by JYOTI. Cell. Mol. Neurobiol. (0272-4340) 2011 Only protein-protein interactions imex curation Dyslexia-associated kiaa0319-like protein interacts with axon guidance receptor nogo receptor 1. Dyslexia-associated kiaa0319-like protein interacts with axon guidance receptor nogo receptor 1. Cell. Mol. Neurobiol. (0272-4340) 2011 imex curation Poon M.W., Tsang W.H., Chan S.O., Li H.M., Ng H.K., Waye M.M. Positive control interaction could not be added as the species of the proteins could not be traced. mary-waye@cuhk.edu.hk 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions human-brain-cortex Homo sapiens brain cortex brain_cortex Brain cortex [Cerebral cortex; Cerebrum cortex; Cortex; Pallium] confocal microscopy confocal microscopy immunostaining immunostaining Poon M.W., Tsang W.H., Chan S.O., Li H.M., Ng H.K., Waye M.M. Positive control interaction could not be added as the species of the proteins could not be traced. mary-waye@cuhk.edu.hk Accepted 2011-NOV-10 by MGT 21-Dec-2011: Contacted by JYOTI. Cell. Mol. Neurobiol. (0272-4340) 2011 Only protein-protein interactions imex curation Dyslexia-associated kiaa0319-like protein interacts with axon guidance receptor nogo receptor 1. Dyslexia-associated kiaa0319-like protein interacts with axon guidance receptor nogo receptor 1. Cell. Mol. Neurobiol. (0272-4340) 2011 imex curation Poon M.W., Tsang W.H., Chan S.O., Li H.M., Ng H.K., Waye M.M. Positive control interaction could not be added as the species of the proteins could not be traced. mary-waye@cuhk.edu.hk 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Poon M.W., Tsang W.H., Chan S.O., Li H.M., Ng H.K., Waye M.M. Positive control interaction could not be added as the species of the proteins could not be traced. mary-waye@cuhk.edu.hk Accepted 2011-NOV-10 by MGT Human brain cDNA Cell. Mol. Neurobiol. (0272-4340) 2011 Only protein-protein interactions imex curation k319l_human Dyslexia-associated protein KIAA0319-like protein KIAA0319L KIAA1837 PP791 AAVR Adeno-associated virus receptor protein protein human Homo sapiens Human MEKRLGVKPNPASWILSGYYWQTSAKWLRSLYLFYTCFCFSVLWLSTDASESRCQQGKTQFGVGLRSGGENHLWLLEGTPSLQSCWAACCQDSACHVFWWLEGMCIQADCSRPQSCRAFRTHSSNSMLVFLKKFQTADDLGFLPEDDVPHLLGLGWNWASWRQSPPRAALRPAVSSSDQQSLIRKLQKRGSPSDVVTPIVTQHSKVNDSNELGGLTTSGSAEVHKAITISSPLTTDLTAELSGGPKNVSVQPEISEGLATTPSTQQVKSSEKTQIAVPQPVAPSYSYATPTPQASFQSTSAPYPVIKELVVSAGESVQITLPKNEVQLNAYVLQEPPKGETYTYDWQLITHPRDYSGEMEGKHSQILKLSKLTPGLYEFKVIVEGQNAHGEGYVNVTVKPEPRKNRPPIAIVSPQFQEISLPTTSTVIDGSQSTDDDKIVQYHWEELKGPLREEKISEDTAILKLSKLVPGNYTFSLTVVDSDGATNSTTANLTVNKAVDYPPVANAGPNQVITLPQNSITLFGNQSTDDHGITSYEWSLSPSSKGKVVEMQGVRTPTLQLSAMQEGDYTYQLTVTDTIGQQATAQVTVIVQPENNKPPQADAGPDKELTLPVDSTTLDGSKSSDDQKIISYLWEKTQGPDGVQLENANSSVATVTGLQVGTYVFTLTVKDERNLQSQSSVNVIVKEEINKPPIAKITGNVVITLPTSTAELDGSKSSDDKGIVSYLWTRDEGSPAAGEVLNHSDHHPILFLSNLVEGTYTFHLKVTDAKGESDTDRTTVEVKPDPRKNNLVEIILDINVSQLTERLKGMFIRQIGVLLGVLDSDIIVQKIQPYTEQSTKMVFFVQNEPPHQIFKGHEVAAMLKSELRKQKADFLIFRALEVNTVTCQLNCSDHGHCDSFTKRCICDPFWMENFIKVQLRDGDSNCEWSVLYVIIATFVIVVALGILSWTVICCCKRQKGKPKRKSKYKILDATDQESLELKPTSRAGIKQKGLLLSSSLMHSESELDSDDAIFTWPDREKGKLLHGQNGSVPNGQTPLKARSPREEIL FD81BFCA3D38D106 rtn4r_human Reticulon-4 receptor RTN4R NOGOR Nogo receptor Nogo-66 receptor UNQ330/PRO526 protein protein human Homo sapiens Human MKRASAGGSRLLAWVLWLQAWQVAAPCPGACVCYNEPKVTTSCPQQGLQAVPVGIPAASQRIFLHGNRISHVPAASFRACRNLTILWLHSNVLARIDAAAFTGLALLEQLDLSDNAQLRSVDPATFHGLGRLHTLHLDRCGLQELGPGLFRGLAALQYLYLQDNALQALPDDTFRDLGNLTHLFLHGNRISSVPERAFRGLHSLDRLLLHQNRVAHVHPHAFRDLGRLMTLYLFANNLSALPTEALAPLRALQYLRLNDNPWVCDCRARPLWAWLQKFRGSSSEVPCSLPQRLAGRDLKRLAANDLQGCAVATGPYHPIWTGRATDEEPLGLPKCCQPDAADKASVLEPGRPASAGNALKGRVPPGDSPPGNGSGPRHINDSPFGTLPGSAEPPLTAVRPEGSEPPGFPTSGPRRRPGCSRKNRTRSHCRLGQAGSGGGGTGDSEGSGALPSLTCSLTPLGLALVLWTVLGPC CA5624B24C584702 kiaa0319l-rtn4r 4 5 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey physical association physical association kiaa0319l-rtn4r-1 1 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 1 kiaa0319l-rtn4r-2 2 5 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 3 kiaa0319l-rtn4r-4 3 6 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 4