UniProt UniProt http://www.uniprot.org Transcriptional corepressor SHP recruits SIRT1 histone deacetylase to inhibit LRH-1 transactivation. Transcriptional corepressor SHP recruits SIRT1 histone deacetylase to inhibit LRH-1 transactivation. Nucleic Acids Res. (0305-1048) 2010 mimix curation Chanda D., Xie Y.B., Choi H.S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of NR0B2/SHP as human according to described pEBG-SHP plasmids (pmid 18657049 and 14752053) though the used sequence numbering of the deletion constructs would fit to mouse or rat origin. Origin of HNF4-alpha according pmid 19651776. Origin of FLAG-SIRT1 according numbering of deletion constructs , myc-SIRT1 according pmid 19690166. hsc@chonnam.ac.kr 14-Mar-2012: Contacted by JYOTI. human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Chanda D., Xie Y.B., Choi H.S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of NR0B2/SHP as human according to described pEBG-SHP plasmids (pmid 18657049 and 14752053) though the used sequence numbering of the deletion constructs would fit to mouse or rat origin. Origin of HNF4-alpha according pmid 19651776. Origin of FLAG-SIRT1 according numbering of deletion constructs , myc-SIRT1 according pmid 19690166. hsc@chonnam.ac.kr Accepted 2011-DEC-02 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Nucleic Acids Res. (0305-1048) 2010 mimix curation Transcriptional corepressor SHP recruits SIRT1 histone deacetylase to inhibit LRH-1 transactivation. Transcriptional corepressor SHP recruits SIRT1 histone deacetylase to inhibit LRH-1 transactivation. Nucleic Acids Res. (0305-1048) 2010 mimix curation Chanda D., Xie Y.B., Choi H.S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of NR0B2/SHP as human according to described pEBG-SHP plasmids (pmid 18657049 and 14752053) though the used sequence numbering of the deletion constructs would fit to mouse or rat origin. Origin of HNF4-alpha according pmid 19651776. Origin of FLAG-SIRT1 according numbering of deletion constructs , myc-SIRT1 according pmid 19690166. hsc@chonnam.ac.kr 14-Mar-2012: Contacted by JYOTI. mouse-liver Mus musculus liver liver anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chanda D., Xie Y.B., Choi H.S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of NR0B2/SHP as human according to described pEBG-SHP plasmids (pmid 18657049 and 14752053) though the used sequence numbering of the deletion constructs would fit to mouse or rat origin. Origin of HNF4-alpha according pmid 19651776. Origin of FLAG-SIRT1 according numbering of deletion constructs , myc-SIRT1 according pmid 19690166. hsc@chonnam.ac.kr Accepted 2011-DEC-02 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Nucleic Acids Res. (0305-1048) 2010 mimix curation Transcriptional corepressor SHP recruits SIRT1 histone deacetylase to inhibit LRH-1 transactivation. Transcriptional corepressor SHP recruits SIRT1 histone deacetylase to inhibit LRH-1 transactivation. Nucleic Acids Res. (0305-1048) 2010 mimix curation Chanda D., Xie Y.B., Choi H.S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of NR0B2/SHP as human according to described pEBG-SHP plasmids (pmid 18657049 and 14752053) though the used sequence numbering of the deletion constructs would fit to mouse or rat origin. Origin of HNF4-alpha according pmid 19651776. Origin of FLAG-SIRT1 according numbering of deletion constructs , myc-SIRT1 according pmid 19690166. hsc@chonnam.ac.kr 14-Mar-2012: Contacted by JYOTI. human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chanda D., Xie Y.B., Choi H.S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of NR0B2/SHP as human according to described pEBG-SHP plasmids (pmid 18657049 and 14752053) though the used sequence numbering of the deletion constructs would fit to mouse or rat origin. Origin of HNF4-alpha according pmid 19651776. Origin of FLAG-SIRT1 according numbering of deletion constructs , myc-SIRT1 according pmid 19690166. hsc@chonnam.ac.kr Accepted 2011-DEC-02 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Nucleic Acids Res. (0305-1048) 2010 mimix curation Transcriptional corepressor SHP recruits SIRT1 histone deacetylase to inhibit LRH-1 transactivation. Transcriptional corepressor SHP recruits SIRT1 histone deacetylase to inhibit LRH-1 transactivation. Nucleic Acids Res. (0305-1048) 2010 mimix curation Chanda D., Xie Y.B., Choi H.S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of NR0B2/SHP as human according to described pEBG-SHP plasmids (pmid 18657049 and 14752053) though the used sequence numbering of the deletion constructs would fit to mouse or rat origin. Origin of HNF4-alpha according pmid 19651776. Origin of FLAG-SIRT1 according numbering of deletion constructs , myc-SIRT1 according pmid 19690166. hsc@chonnam.ac.kr 14-Mar-2012: Contacted by JYOTI. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. pull down pull down western blot western blot Immuno blot Immuno blot Chanda D., Xie Y.B., Choi H.S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of NR0B2/SHP as human according to described pEBG-SHP plasmids (pmid 18657049 and 14752053) though the used sequence numbering of the deletion constructs would fit to mouse or rat origin. Origin of HNF4-alpha according pmid 19651776. Origin of FLAG-SIRT1 according numbering of deletion constructs , myc-SIRT1 according pmid 19690166. hsc@chonnam.ac.kr Accepted 2011-DEC-02 by ORCHARD Nucleic Acids Res. (0305-1048) 2010 mimix curation Transcriptional corepressor SHP recruits SIRT1 histone deacetylase to inhibit LRH-1 transactivation. Transcriptional corepressor SHP recruits SIRT1 histone deacetylase to inhibit LRH-1 transactivation. Nucleic Acids Res. (0305-1048) 2010 mimix curation Chanda D., Xie Y.B., Choi H.S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of NR0B2/SHP as human according to described pEBG-SHP plasmids (pmid 18657049 and 14752053) though the used sequence numbering of the deletion constructs would fit to mouse or rat origin. Origin of HNF4-alpha according pmid 19651776. Origin of FLAG-SIRT1 according numbering of deletion constructs , myc-SIRT1 according pmid 19690166. hsc@chonnam.ac.kr 14-Mar-2012: Contacted by JYOTI. human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). pull down pull down western blot western blot Immuno blot Immuno blot Chanda D., Xie Y.B., Choi H.S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of NR0B2/SHP as human according to described pEBG-SHP plasmids (pmid 18657049 and 14752053) though the used sequence numbering of the deletion constructs would fit to mouse or rat origin. Origin of HNF4-alpha according pmid 19651776. Origin of FLAG-SIRT1 according numbering of deletion constructs , myc-SIRT1 according pmid 19690166. hsc@chonnam.ac.kr Accepted 2011-DEC-02 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Nucleic Acids Res. (0305-1048) 2010 mimix curation Transcriptional corepressor SHP recruits SIRT1 histone deacetylase to inhibit LRH-1 transactivation. Transcriptional corepressor SHP recruits SIRT1 histone deacetylase to inhibit LRH-1 transactivation. Nucleic Acids Res. (0305-1048) 2010 mimix curation Chanda D., Xie Y.B., Choi H.S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of NR0B2/SHP as human according to described pEBG-SHP plasmids (pmid 18657049 and 14752053) though the used sequence numbering of the deletion constructs would fit to mouse or rat origin. Origin of HNF4-alpha according pmid 19651776. Origin of FLAG-SIRT1 according numbering of deletion constructs , myc-SIRT1 according pmid 19690166. hsc@chonnam.ac.kr 14-Mar-2012: Contacted by JYOTI. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging anti tag immunost anti tag immunostaining anti tag immunost Chanda D., Xie Y.B., Choi H.S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of NR0B2/SHP as human according to described pEBG-SHP plasmids (pmid 18657049 and 14752053) though the used sequence numbering of the deletion constructs would fit to mouse or rat origin. Origin of HNF4-alpha according pmid 19651776. Origin of FLAG-SIRT1 according numbering of deletion constructs , myc-SIRT1 according pmid 19690166. hsc@chonnam.ac.kr Accepted 2011-DEC-02 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Nucleic Acids Res. (0305-1048) 2010 mimix curation nr0b2_mouse Nuclear receptor subfamily 0 group B member 2 Nr0b2 Shp Orphan nuclear receptor SHP Small heterodimer partner protein protein mouse Mus musculus Mouse MSSGQSGVCPCQGSAGRPTILYALLSPSPRTRPVAPASHSHCLCQQQRPVRLCAPHRTCREALDVLAKTVAFLRNLPSFCHLPHEDQRRLLECCWGPLFLLGLAQDAVTFEVAEAPVPSILKKILLEEASSGTQGAQPSDRPQPSLAAVQWLQRCLESFWSLELGPKEYAYLKGTILFNPDVPGLRASCHIAHLQQEAHWALCEVLEPWYPASQGRLARILLMASTLKNIPGTLLVDLFFRPIMGDVDITELLEDMLLLR 7B859325AA73DCA1 hnf4a_rat Hepatocyte nuclear factor 4-alpha Hnf4a Hnf-4 Hnf4 Nr2a1 Tcf14 Nuclear receptor subfamily 2 group A member 1 Transcription factor 14 Transcription factor HNF-4 protein protein rat Rattus norvegicus (Rat) Rat MRLSKTLADMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGANLNSSNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSQQITSPISGINGDIRAKKIANITDVCESMKEQLLVLVEWAKYIPAFCELLLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYACLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSASDAPHAHHPLHPHLMQEHMGTNVIVANTMPSHLSNGQMCEWPRPRGQAATPETPQPSPPSGSGSESYKLLPGAITTIVKPPSAIPQPTITKQEAI E6DECA74FEA65E75 nr0b2_human Nuclear receptor subfamily 0 group B member 2 NR0B2 SHP Orphan nuclear receptor SHP Small heterodimer partner protein protein human Homo sapiens Human MSTSQPGACPCQGAASRPAILYALLSSSLKAVPRPRSRCLCRQHRPVQLCAPHRTCREALDVLAKTVAFLRNLPSFWQLPPQDQRRLLQGCWGPLFLLGLAQDAVTFEVAEAPVPSILKKILLEEPSSSGGSGQLPDRPQPSLAAVQWLQCCLESFWSLELSPKEYACLKGTILFNPDVPGLQAASHIGHLQQEAHWVLCEVLEPWCPAAQGRLTRVLLTASTLKSIPTSLLGDLFFRPIIGDVDIAGLLGDMLLLR DIP protein Q15466 original sequence version: 79 DIP protein Q15466 original sequence version: 113 14BEE2B3FF46154A sir1_mouse NAD-dependent protein deacetylase sirtuin-1 NAD-dependent protein deacylase sirtuin-1 Sirt1 Sir2l1 SIR2alpha SIR2-like protein 1 Regulatory protein SIR2 homolog 1 protein protein mouse Mus musculus Mouse MADEVALALQAAGSPSAAAAMEAASQPADEPLRKRPRRDGPGLGRSPGEPSAAVAPAAAGCEAASAAAPAALWREAAGAAASAEREAPATAVAGDGDNGSGLRREPRAADDFDDDEGEEEDEAAAAAAAAAIGYRDNLLLTDGLLTNGFHSCESDDDDRTSHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRILQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRPQKELVHLSELPPTPLHISEDSSSPERTVPQDSSVIATLVDQATNNNVNDLEVSESSCVEEKPQEVQTSRNVENINVENPDFKAVGSSTADKNERTSVAETVRKCWPNRLAKEQISKRLEGNQYLFVPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPLEDESEIEEFYNGLEDDTERPECAGGSGFGADGGDQEVVNEAIATRQELTDVNYPSDKS DIP protein Q923E4 original sequence version: 83 DIP protein Q923E4 original sequence version: 135 7F15625E29433119 sir1_human NAD-dependent protein deacetylase sirtuin-1 NAD-dependent protein deacylase sirtuin-1 SIRT1 SIR2L1 SIR2-like protein 1 Regulatory protein SIR2 homolog 1 protein protein human Homo sapiens Human MADEAALALQPGGSPSAAGADREAASSPAGEPLRKRPRRDGPGLERSPGEPGGAAPEREVPAAARGCPGAAAAALWREAEAEAAAAGGEQEAQATAAAGEGDNGPGLQGPSREPPLADNLYDEDDDDEGEEEEEAAAAAIGYRDNLLFGDEIITNGFHSCESDEEDRASHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRTQKELAYLSELPPTPLHVSEDSSSPERTSPPDSSVIVTLLDQAAKSNDDLDVSESKGCMEEKPQEVQTSRNVESIAEQMENPDLKNVGSSTGEKNERTSVAGTVRKCWPNRVAKEQISRRLDGNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESEIEEFYNGLEDEPDVPERAGGAGFGTDGDDQEAINEAISVKQEVTDMNYPSNKS DIP protein Q96EB6 original sequence version: 140 DIP protein Q96EB6 original sequence version: 71 DIP protein Q96EB6 original sequence version: 125 DIP protein Q96EB6 original sequence version: 115 DIP protein Q96EB6 original sequence version: 63 DIP protein Q96EB6-1 original sequence version: 102 DIP protein Q96EB6 original sequence version: 102 DIP protein Q96EB6 original sequence version: 98 DIP protein Q96EB6 original sequence version: 144 DIP protein Q96EB6 original sequence version: 120 DIP protein Q96EB6-1 original sequence version: 120 DIP protein Q96EB6 original sequence version: 113 DIP protein Q96EB6-1 original sequence version: 117 2D3BEA6D73DA229F nr0b2-sirt1 4 9 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association 2A nr0b2-sirt1-1 2 7 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association 2B sirt1-nr0b2 2 7 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association 2B nr0b2-sirt1-2 3 9 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association 2B sirt1-nr0b2-1 3 9 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association 2B nr0b2-sirt1-3 6 11 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component colocalization colocalization 2C nr0b2-sirt1-4 5 9 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association 3A, B C and D nr0b2-sirt1-5 1 9 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association 4B hnf4a-sirt1 1 8 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association 4F