MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it PTEN inhibits BMI1 function independently of its phosphatase activity. PTEN inhibits BMI1 function independently of its phosphatase activity. Mol. Cancer 2009 imex curation Fan C., He L., Kapoor A., Rybak AP., De Melo J., Cutz JC., Tang D. suggested by lperfetto fanc3@mcmaster.ca 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions partial coverage imex curation rapid curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Fan C., He L., Kapoor A., Rybak AP., De Melo J., Cutz JC., Tang D. suggested by lperfetto fanc3@mcmaster.ca Accepted 2013-SEP-10 by MARTA 30-Sept-2013: Contacted by IntAct-Help. Mol. Cancer 2009 IMEx PTEN inhibits BMI1 function independently of its phosphatase activity. PTEN inhibits BMI1 function independently of its phosphatase activity. Mol. Cancer 2009 imex curation Fan C., He L., Kapoor A., Rybak AP., De Melo J., Cutz JC., Tang D. suggested by lperfetto fanc3@mcmaster.ca 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions partial coverage imex curation rapid curation human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Fan C., He L., Kapoor A., Rybak AP., De Melo J., Cutz JC., Tang D. suggested by lperfetto fanc3@mcmaster.ca 30-Sept-2013: Contacted by IntAct-Help. Mol. Cancer 2009 IMEx PTEN inhibits BMI1 function independently of its phosphatase activity. PTEN inhibits BMI1 function independently of its phosphatase activity. Mol. Cancer 2009 imex curation Fan C., He L., Kapoor A., Rybak AP., De Melo J., Cutz JC., Tang D. suggested by lperfetto fanc3@mcmaster.ca 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions partial coverage imex curation rapid curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Fan C., He L., Kapoor A., Rybak AP., De Melo J., Cutz JC., Tang D. suggested by lperfetto fanc3@mcmaster.ca Accepted 2013-SEP-10 by MARTA 30-Sept-2013: Contacted by IntAct-Help. Mol. Cancer 2009 IMEx PTEN inhibits BMI1 function independently of its phosphatase activity. PTEN inhibits BMI1 function independently of its phosphatase activity. Mol. Cancer 2009 imex curation Fan C., He L., Kapoor A., Rybak AP., De Melo J., Cutz JC., Tang D. suggested by lperfetto fanc3@mcmaster.ca 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions partial coverage imex curation rapid curation human-du_145 Homo sapiens DU-145 prostate cancer cell line du_145 DU-145 Human prostate carcinoma established from the tumor tissue removed from the metastatic central nervous system lesion of a 69-year-old man with prostate carcinoma in 1975 confirmed as human with IEF of AST, MDH, NP crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Fan C., He L., Kapoor A., Rybak AP., De Melo J., Cutz JC., Tang D. suggested by lperfetto fanc3@mcmaster.ca Accepted 2013-SEP-10 by MARTA 30-Sept-2013: Contacted by IntAct-Help. Mol. Cancer 2009 IMEx PTEN inhibits BMI1 function independently of its phosphatase activity. PTEN inhibits BMI1 function independently of its phosphatase activity. Mol. Cancer 2009 imex curation Fan C., He L., Kapoor A., Rybak AP., De Melo J., Cutz JC., Tang D. suggested by lperfetto fanc3@mcmaster.ca 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions partial coverage imex curation rapid curation human-du_145 Homo sapiens DU-145 prostate cancer cell line du_145 DU-145 Human prostate carcinoma established from the tumor tissue removed from the metastatic central nervous system lesion of a 69-year-old man with prostate carcinoma in 1975 confirmed as human with IEF of AST, MDH, NP fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Fan C., He L., Kapoor A., Rybak AP., De Melo J., Cutz JC., Tang D. suggested by lperfetto fanc3@mcmaster.ca 30-Sept-2013: Contacted by IntAct-Help. Mol. Cancer 2009 IMEx PTEN inhibits BMI1 function independently of its phosphatase activity. PTEN inhibits BMI1 function independently of its phosphatase activity. Mol. Cancer 2009 imex curation Fan C., He L., Kapoor A., Rybak AP., De Melo J., Cutz JC., Tang D. suggested by lperfetto fanc3@mcmaster.ca 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions partial coverage imex curation rapid curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Fan C., He L., Kapoor A., Rybak AP., De Melo J., Cutz JC., Tang D. suggested by lperfetto fanc3@mcmaster.ca Accepted 2013-SEP-10 by MARTA 30-Sept-2013: Contacted by IntAct-Help. Mol. Cancer 2009 IMEx bmi1_human Polycomb complex protein BMI-1 BMI1 PCGF4 RNF51 Polycomb group RING finger protein 4 RING finger protein 51 protein protein human Homo sapiens Human MHRTTRIKITELNPHLMCVLCGGYFIDATTIIECLHSFCKTCIVRYLETSKYCPICDVQVHKTRPLLNIRSDKTLQDIVYKLVPGLFKNEMKRRRDFYAAHPSADAANGSNEDRGEVADEDKRIITDDEIISLSIEFFDQNRLDRKVNKDKEKSKEEVNDKRYLRCPAAMTVMHLRKFLRSKMDIPNTFQIDVMYEEEPLKDYYTLMDIAYIYTWRRNGPLPLKYRVRPTCKRMKISHQRDGLTNAGELESDSGSDKANSPAGGIPSTSSCLPSPSTPVQSPHPQFPHISSTMNGTSNSPSGNHQSSFANRPRKSSVNGSSATSSG DIP protein P35226 original sequence version: 105 DIP protein P35226 original sequence version: 136 DIP protein P35226 original sequence version: 110 DIP protein P35226 original sequence version: 129 030A7D396BADA543 pten_human Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN Phosphatase and tensin homolog Mutated in multiple advanced cancers 1 PTEN MMAC1 TEP1 protein protein human Homo sapiens Human MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHTQITKV 75F97C3DD6802BA9 a0a2i3tnh3_pantr Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN Phosphatase and tensin homolog PTEN protein protein pantr Pan troglodytes (Chimpanzee) Chimpanzee MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHTQITKV 3695A464F5D7BE40 pten-bmi1-2 6 8 unspecified role unspecified role bait bait binding site sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P60484:p.Cys124Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C S Wed May 02 19:49:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys124ser' Tue Jun 30 17:43:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys124Ser' P60484:p.Gly129Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G E Wed May 02 17:38:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly129glu' Tue Jun 30 16:47:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly129Glu' 7 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association homomint mint f1a f2a bmi1-pten-2 1 8 unspecified role unspecified role prey prey binding site sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association homomint mint f2cd bmi1-pten-1 4 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association f1b homomint mint akt1-pten-3 1 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Resulting Phenotypic qualities (properties)[PATO]: PATO:0002263 (dephosphorylated) 9 unspecified role unspecified role prey prey under-expressed under expressed level under-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain A0A2I3TNH3:p.Lys13Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Wed Jun 13 19:03:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys13ala' Tue Jun 30 18:48:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys13Ala' physical association physical association f4 homomint mint bmi1-pten-4 3 7 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Resulting Phenotypic qualities (properties)[PATO]: PATO:0002263 (dephosphorylated) 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization f1c homomint mint bmi1-pten-3 1 8 unspecified role unspecified role prey prey binding site sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P60484:p.Cys124Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C S Wed May 02 16:27:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys124ser' Tue Jun 30 19:20:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys124Ser' P60484:p.Gly129Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G E Wed May 02 18:30:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly129glu' Tue Jun 30 20:00:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly129Glu' 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association homomint mint f1a f2a bmi1-pten-5 5 7 unspecified role unspecified role neutral component neutral component Resulting Phenotypic qualities (properties)[PATO]: PATO:0002263 (dephosphorylated) 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed colocalization colocalization f1c homomint mint bmi1-pten-6 2 7 unspecified role unspecified role neutral component neutral component Resulting Phenotypic qualities (properties)[PATO]: PATO:0002263 (dephosphorylated) 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed colocalization colocalization f1c homomint mint