UniProt UniProt http://www.uniprot.org A competition between stimulators and antagonists of Upf complex recruitment governs human nonsense-mediated mRNA decay. A competition between stimulators and antagonists of Upf complex recruitment governs human nonsense-mediated mRNA decay. PLoS Biol. (1544-9173) 2008 mimix curation Singh G., Rebbapragada I., Lykke-Andersen J. The used antibody to precipitate the bait protein is not specific and cannot differentiate between eRF3a and eRF3b. eRF3b/GSPT2 (AC Q8IYD1) is indicated here as further experiments with transfected plasmids support its participation. Jens.Lykke-Andersen@colorado.edu 06-Jun-2011: Contacted by JYOTI. orchard human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Singh G., Rebbapragada I., Lykke-Andersen J. The used antibody to precipitate the bait protein is not specific and cannot differentiate between eRF3a and eRF3b. eRF3b/GSPT2 (AC Q8IYD1) is indicated here as further experiments with transfected plasmids support its participation. Jens.Lykke-Andersen@colorado.edu Accepted 2011-APR-27 by ORCHARD 06-Jun-2011: Contacted by JYOTI. PLoS Biol. (1544-9173) 2008 orchard mimix curation A competition between stimulators and antagonists of Upf complex recruitment governs human nonsense-mediated mRNA decay. A competition between stimulators and antagonists of Upf complex recruitment governs human nonsense-mediated mRNA decay. PLoS Biol. (1544-9173) 2008 mimix curation Singh G., Rebbapragada I., Lykke-Andersen J. The used antibody to precipitate the bait protein is not specific and cannot differentiate between eRF3a and eRF3b. eRF3b/GSPT2 (AC Q8IYD1) is indicated here as further experiments with transfected plasmids support its participation. Jens.Lykke-Andersen@colorado.edu 06-Jun-2011: Contacted by JYOTI. orchard human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Singh G., Rebbapragada I., Lykke-Andersen J. The used antibody to precipitate the bait protein is not specific and cannot differentiate between eRF3a and eRF3b. eRF3b/GSPT2 (AC Q8IYD1) is indicated here as further experiments with transfected plasmids support its participation. Jens.Lykke-Andersen@colorado.edu Accepted 2011-APR-27 by ORCHARD 06-Jun-2011: Contacted by JYOTI. PLoS Biol. (1544-9173) 2008 orchard mimix curation A competition between stimulators and antagonists of Upf complex recruitment governs human nonsense-mediated mRNA decay. A competition between stimulators and antagonists of Upf complex recruitment governs human nonsense-mediated mRNA decay. PLoS Biol. (1544-9173) 2008 mimix curation Singh G., Rebbapragada I., Lykke-Andersen J. The used antibody to precipitate the bait protein is not specific and cannot differentiate between eRF3a and eRF3b. eRF3b/GSPT2 (AC Q8IYD1) is indicated here as further experiments with transfected plasmids support its participation. Jens.Lykke-Andersen@colorado.edu 06-Jun-2011: Contacted by JYOTI. orchard in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Singh G., Rebbapragada I., Lykke-Andersen J. The used antibody to precipitate the bait protein is not specific and cannot differentiate between eRF3a and eRF3b. eRF3b/GSPT2 (AC Q8IYD1) is indicated here as further experiments with transfected plasmids support its participation. Jens.Lykke-Andersen@colorado.edu Accepted 2011-APR-27 by ORCHARD 06-Jun-2011: Contacted by JYOTI. PLoS Biol. (1544-9173) 2008 orchard mimix curation A competition between stimulators and antagonists of Upf complex recruitment governs human nonsense-mediated mRNA decay. A competition between stimulators and antagonists of Upf complex recruitment governs human nonsense-mediated mRNA decay. PLoS Biol. (1544-9173) 2008 mimix curation Singh G., Rebbapragada I., Lykke-Andersen J. The used antibody to precipitate the bait protein is not specific and cannot differentiate between eRF3a and eRF3b. eRF3b/GSPT2 (AC Q8IYD1) is indicated here as further experiments with transfected plasmids support its participation. Jens.Lykke-Andersen@colorado.edu 06-Jun-2011: Contacted by JYOTI. orchard human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Singh G., Rebbapragada I., Lykke-Andersen J. The used antibody to precipitate the bait protein is not specific and cannot differentiate between eRF3a and eRF3b. eRF3b/GSPT2 (AC Q8IYD1) is indicated here as further experiments with transfected plasmids support its participation. Jens.Lykke-Andersen@colorado.edu Accepted 2011-APR-27 by ORCHARD 06-Jun-2011: Contacted by JYOTI. PLoS Biol. (1544-9173) 2008 orchard mimix curation erf1_human Eukaryotic peptide chain release factor subunit 1 ETF1 ERF1 Protein Cl1 RF1 SUP45L1 TB3-1 protein protein human Homo sapiens Human MADDPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVIDGSGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLFISGDKVNVAGLVLAGSADFKTELSQSDMFDQRLQSKVLKLVDISYGGENGFNQAIELSTEVLSNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLKALEMGAVEILIVYENLDIMRYVLHCQGTEEEKILYLTPEQEKDKSHFTDKETGQEHELIESMPLLEWFANNYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGMEYQGGDDEFFDLDDY CECC50D100E59D19 rent1_human Regulator of nonsense transcripts 1 UPF1 RENT1 KIAA0221 ATP-dependent helicase RENT1 Nonsense mRNA reducing factor 1 Up-frameshift suppressor 1 homolog protein protein human Homo sapiens Human MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGPGGGGAGGPGGAGAGAAAGQLDAQVGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPIHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSGNEDLVIIWLRDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSSMYFQTHDQIGMISAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGLPGPSQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY DIP protein Q92900 original sequence version: 137 DIP protein Q92900 original sequence version: 76 DIP protein Q92900 original sequence version: 101 DIP protein Q92900 original sequence version: 102 DIP protein Q92900 original sequence version: 135 DIP protein Q92900-1 original sequence version: 123 DIP protein Q92900 original sequence version: 132 6CCA6FE42B15BA28 erf3b_human Eukaryotic peptide chain release factor GTP-binding subunit ERF3B GSPT2 ERF3B G1 to S phase transition protein 2 homolog protein protein human Homo sapiens Human MDSGSSSSDSAPDCWDQVDMESPGSAPSGDGVSSAVAEAQREPLSSAFSRKLNVNAKPFVPNVHAAEFVPSFLRGPTQPPTLPAGSGSNDETCTGAGYPQGKRMGRGAPVEPSREEPLVSLEGSNSAVTMELSEPVVENGEVEMALEESWEHSKEVSEAEPGGGSSGDSGPPEESGQEMMEEKEEIRKSKSVIVPSGAPKKEHVNVVFIGHVDAGKSTIGGQIMFLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETERKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSIERYEECKEKLVPFLKKVGFSPKKDIHFMPCSGLTGANIKEQSDFCPWYTGLPFIPYLDNLPNFNRSIDGPIRLPIVDKYKDMGTVVLGKLESGSIFKGQQLVMMPNKHNVEVLGILSDDTETDFVAPGENLKIRLKGIEEEEILPGFILCDPSNLCHSGRTFDVQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALISLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD pabp1_human Polyadenylate-binding protein 1 PABPC1 PAB1 PABP1 PABPC2 PABP protein protein human Homo sapiens Human MNPSAPSYPMASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYGFVHFETQEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAELGARAKEFTNVYIKNFGEDMDDERLKDLFGKFGPALSVKVMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRKFEQMKQDRITRYQGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKVMMEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQRKEERQAHLTNQYMQRMASVRAVPNPVINPYQPAPPSGYFMAAIPQTQNRAAYYPPSQIAQLRPSPRWTAQGARPHPFQNMPGAIRPAAPRPPFSTMRPASSQVPRVMSTQRVANTSTQTMGPRPAAAAAAATPAVRTVPQYKYAAGVRNPQQHLNAQPQVTMQQPAVHVQGQEPLTASMLASAPPQEQKQMLGERLFPLIQAMHPTLAGKITGMLLEIDNSELLHMLESPESLRSKVDEAVAVLQAHQAKEAAQKAVNSATGVPTV DIP protein P11940 original sequence version: 187 DIP protein P11940 original sequence version: 181 DIP protein P11940-1 original sequence version: 136 DIP protein P11940 original sequence version: 148 DIP protein P11940 original sequence version: 172 2EB1B02A346132EE erf3b-pabpc1 1 7 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey association association 6A erf3b-upf1 3 7 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association 6B UPF1 can be out-competed by PABPC1. erf3b-pabpc1-1 3 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association 6B pabpc1-erf3b 4 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association 6C upf1-erf3b 4 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association 6c erf3b-etf1 2 7 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association 6C