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  <entry>
    <source releaseDate="2022-02-04">
      <names>
        <shortLabel>UniProt</shortLabel>
        <fullName>UniProt</fullName>
      </names>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0486" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-3989247" refType="identity" refTypeAc="MI:0356"/>
      </xref>
      <attributeList>
        <attribute name="url" nameAc="MI:0614">http://www.uniprot.org</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Structure and mechanism of spermidine synthases.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="17585781" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-4292768" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="imex" dbAc="MI:0670" id="IM-17048" refType="imex-primary" refTypeAc="MI:0662"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Structure and mechanism of spermidine synthases.</attribute>
            <attribute name="journal" nameAc="MI:0885">Biochemistry (0006-2960)</attribute>
            <attribute name="publication year" nameAc="MI:0886">2007</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Wu H., Min J., Ikeguchi Y., Zeng H., Dong A., Loppnau P., Pegg A.E., Plotnikov A.N.</attribute>
            <attribute name="contact-email" nameAc="MI:0634">alexander.plotnikov@utoronto.ca</attribute>
            <attribute name="author-announcement">26-Sep-2011: Contacted by JYOTI.</attribute>
            <attribute name="reviewer">orchard</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="pubmed" dbAc="MI:0446" id="17585781" refType="primary-reference" refTypeAc="MI:0358"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-17048" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="-1">
            <names>
              <shortLabel>in vitro</shortLabel>
              <fullName>In vitro</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>x-ray diffraction</shortLabel>
            <fullName>x-ray crystallography</fullName>
            <alias type="go synonym" typeAc="MI:0303">X-ray</alias>
            <alias type="synonym" typeAc="MI:1041">X-ray</alias>
            <alias type="synonym" typeAc="MI:1041">x-ray diffraction</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0114" refType="identity" refTypeAc="MI:0356"/>
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          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
            <alias type="synonym" typeAc="MI:1041">predetermined</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Wu H., Min J., Ikeguchi Y., Zeng H., Dong A., Loppnau P., Pegg A.E., Plotnikov A.N.</attribute>
          <attribute name="contact-email" nameAc="MI:0634">alexander.plotnikov@utoronto.ca</attribute>
          <attribute name="accepted">Accepted 2011-JUL-04 by ORCHARD</attribute>
          <attribute name="author-announcement">26-Sep-2011: Contacted by JYOTI.</attribute>
          <attribute name="journal" nameAc="MI:0885">Biochemistry (0006-2960)</attribute>
          <attribute name="publication year" nameAc="MI:0886">2007</attribute>
          <attribute name="reviewer">orchard</attribute>
          <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
          <attribute name="imex curation" nameAc="MI:0959"/>
          <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>spee_human</shortLabel>
          <fullName>Spermidine synthase</fullName>
          <alias type="gene name" typeAc="MI:0301">SRM</alias>
          <alias type="gene name synonym" typeAc="MI:0302">SPS1</alias>
          <alias type="gene name synonym" typeAc="MI:0302">SRML1</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Putrescine aminopropyltransferase</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="P19623" version="SP_77" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q15511" version="SP_77" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="B1AKP9" version="SP_104" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSP00000366156" version="SP_208" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1056183" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_003123.2"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSG00000116649" version="SP_208" refType="gene"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENST00000376957" version="SP_208" refType="transcript"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0004766" version="SP_208"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005829" version="SP_208"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0006595" version="SP_208"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0006596" version="SP_208"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0008295" version="SP_208"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0042802" version="SP_208"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0042803" version="SP_208"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:1990830" version="SP_208"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR001045" version="SP_208"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR029063" version="SP_208"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR030373" version="SP_208"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR030374" version="SP_208"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR030668" version="SP_208"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR035246" version="SP_208"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR037163" version="SP_208"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2O05" version="SP_208"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2O06" version="SP_208"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2O07" version="SP_208"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2O0L" version="SP_208"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3RW9" version="SP_208"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-351202" version="SP_208"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="9606">
          <names>
            <shortLabel>human</shortLabel>
            <fullName>Homo sapiens</fullName>
            <alias type="synonym" typeAc="MI:1041">Human</alias>
          </names>
        </organism>
        <sequence>MEPGPDGPAASGPAAIREGWFRETCSLWPGQALSLQVEQLLHHRRSRYQDILVFRSKTYGNVLVLDGVIQCTERDEFSYQEMIANLPLCSHPNPRKVLIIGGGDGGVLREVVKHPSVESVVQCEIDEDVIQVSKKFLPGMAIGYSSSKLTLHVGDGFEFMKQNQDAFDVIITDSSDPMGPAESLFKESYYQLMKTALKEDGVLCCQGECQWLHLDLIKEMRQFCQSLFPVVAYAYCTIPTYPSGQIGFMLCSKNPSTNFQEPVQPLTQQQVAQMQLKYYNSDVHRAAFVLPEFARKALNDVS</sequence>
        <attributeList>
          <attribute name="crc64">3EF454D886F6425D</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="3" imexId="IM-17048-1">
        <names>
          <shortLabel>spee-1</shortLabel>
        </names>
        <xref>
          <primaryRef db="pdbe" dbAc="MI:0472" id="2O07" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-4292782" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-17048-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="4">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <featureList>
              <feature id="5">
                <names>
                  <shortLabel>region</shortLabel>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-4302617" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>his tag</shortLabel>
                    <fullName>his tag</fullName>
                    <alias type="go synonym" typeAc="MI:0303">Hexa-His-tag</alias>
                    <alias type="go synonym" typeAc="MI:0303">6-His-tag</alias>
                    <alias type="go synonym" typeAc="MI:0303">Histidine-tag</alias>
                    <alias type="synonym" typeAc="MI:1041">Hexa-His-tag</alias>
                    <alias type="synonym" typeAc="MI:1041">Histidine-tag</alias>
                    <alias type="synonym" typeAc="MI:1041">6-His-tag</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0521" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456516" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>undetermined</shortLabel>
                        <fullName>undetermined sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">undetermined</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0339" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-448295" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <endStatus>
                      <names>
                        <shortLabel>undetermined</shortLabel>
                        <fullName>undetermined sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">undetermined</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0339" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-448295" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                  </featureRange>
                </featureRangeList>
              </feature>
            </featureList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="83333">
                <names>
                  <shortLabel>ecoli</shortLabel>
                  <fullName>Escherichia coli (strain K12)</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
            <stoichiometry value="2"/>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0407" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608833" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="3d-r-factors" nameAc="MI:0631">R-value 0.179  R free 0.228</attribute>
          <attribute name="3d-resolution" nameAc="MI:0632">1.89 A</attribute>
          <attribute name="comment" nameAc="MI:0612">group deposited 4 structures for this protein, (2O05, 2O06, 2O07, 2O0L), showed R-factors and resolution for the one with the highest resolution =2O07</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>