IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ STAM and Hrs down-regulate ciliary TRP receptors. STAM and Hrs down-regulate ciliary TRP receptors. Mol. Biol. Cell (1059-1524) 2007 imex curation Hu J., Wittekind SG., Barr MM. This experiment relates to a negative interaction. I have never added a negative interaction before - so am not sure if I am following the Manual !00% correctly... barr@biology.rutgers.edu A cDNA library derived from mixed-stage him-5 animals. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Hu J., Wittekind SG., Barr MM. This experiment relates to a negative interaction. I have never added a negative interaction before - so am not sure if I am following the Manual !00% correctly... barr@biology.rutgers.edu Accepted 2011-MAR-30 by JYOTI A cDNA library derived from mixed-stage him-5 animals. Mol. Biol. Cell (1059-1524) 2007 jyoti Only protein-protein interactions imex curation STAM and Hrs down-regulate ciliary TRP receptors. STAM and Hrs down-regulate ciliary TRP receptors. Mol. Biol. Cell (1059-1524) 2007 imex curation Hu J., Wittekind SG., Barr MM. This experiment relates to a negative interaction. I have never added a negative interaction before - so am not sure if I am following the Manual !00% correctly... barr@biology.rutgers.edu A cDNA library derived from mixed-stage him-5 animals. jyoti Only protein-protein interactions caeel-neuron Caenorhabditis elegans neuron neuron Neuron [Neuronal cell; Neural cell confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Hu J., Wittekind SG., Barr MM. This experiment relates to a negative interaction. I have never added a negative interaction before - so am not sure if I am following the Manual !00% correctly... barr@biology.rutgers.edu Accepted 2011-MAR-30 by JYOTI A cDNA library derived from mixed-stage him-5 animals. Mol. Biol. Cell (1059-1524) 2007 jyoti Only protein-protein interactions imex curation o01498-1 Signal transducing adapter molecule 1 stam-1 pqn-19 Prion-like-(Q/N-rich) domain-bearing protein 19 C34G6.7 protein protein caeel Caenorhabditis elegans MKKQKSFPMSAYEDLLGKITAPTITVENWEGILAFCDMINNDFEGSKTGIKSLRKRLNNRDPHVVLLAISVLDSCWANCEERFRKEVSSAQFINELKALCTSSQRQVAEKMRLTVQKWVDTECKTEQSLSLIVTLHKNLVADGYSFVVDDPKSKTKAIDAKFANDPNYVGSAQEEEAIAKAIAASLADAEKQEKAKKSTSTMYPSAKASSPAVQTNSNIPEKNVRALYDFEAAESNELSFVAGDIITITDESNPHWWTGRIGTQQGLFPSSFVTNQLDDLKSKETDSSQKAPEVVASINEAILVRCLQVLHECDPTGERQDPEDLAQLEAASYAQGNLIDAHLASIDRQSNSLAQIDVAIRDVLALYDDAIQKGGFQHQSQGMYQQPMQQYNYQQPPNRAYYPPTGPVQQQQPQQYPPQHYPAPGAQPQYACPPNSVPQPQQQQQQWPAPSSQPQQY No experimental confirmation available. 1AD9B1D83EBE2E16 q17796_caeel Hepatocyte growth factor-regulated tyrosine kinase substrate hgrs-1 CELE_C07G1.5 C07G1.5 protein protein caeel Caenorhabditis elegans MATKFQRVLDQATDSTLVEPNWEGIILCTDMIRSGEVPAKPSLQAIRKRMQHENPHVVNHTLLVLDACVKNCGHKVHAEVATREFMEDFKNLVTENKYDEVKNKSLEMLQCWATAFANKPEYKMVVDTHNLMKLAGFDFPSLKEADAMFMAQVAPEWADGPECYRCRSVFSVFTRKHHCRACGQIFCDKCSSRELALPQFGIEKEVRVCETCYEKKVAEVKERYPAVKKQLDAAHGRKGDSEADKAAKEKLLREKEEEDLALAIAISQSEAEAKEKEKQNNLYSMYNGIKPEVDGYKGAAEPVTAPPPDDSSSDPLARYLNRDYWQQKKEGKVEDWSSRSSALSATAPPPSEPSIAPSICSTLMGPDDNSLNADIAAMSLGGTNGLNGTVSDDAKLQADDTMKWCQSIREQVSVMDNRIRSNLARGRPVFNDSAIQDLFTKLTELHSHVLSRMHTLDEQRGYYESLQDHLANIGEARQAIDEMREEHERKRQERMAEEQRLRQAQMQQTLEMMRMKKHAMLMEQREQALQRFQQQQQEMAMRRHQQQAYYNPQQMGYGAPPPSGPQQPPQQSYYGYQQGPQSQAPPPSSQYQQSHASTQQQQQQYYQHYQANQTVTQPVQQAQQQYQQQYQGYYQQQQPYQQQAGYQNPQQHQNYQNGTTNENGQYQQQQSSEVKQEHQNHGYQQQQAQHPPASVASNGHNGYGNVDQNAHHHQQQQPQIAEQPLISFD 6EBF07508CA9961F pkd1_caeel Location of vulva defective 1 lov-1 pkd-1 Polycystin-1 Polycystic kidney disease 1 protein homolog ZK945.9/ZK945.10 protein protein caeel Caenorhabditis elegans MKKSNFFVLLLLAISAIQIDGLHYQLLDGIATFRLDNDDTTIGGVPRNSQGVVKIKLSCGLNRLSVENKVTEVSSLELIHNCIQTETRLVGLFLNSTWITLNEVNDDDEISIAVEAKYEVCYDDGIDRCDGSLWWLQVGGNEMALLGYREKCESGEINEEYARRMCKRPYRSEKSTAISDSQGVYYDGQVLKGVRAKQFSMRTSGSPTLRRMKRDAGDNTCDYTIESTSTSTTTPTTTTVTSTVTSTTTVPTSTSTVTTAMSTSTSTPSTSTTIESTSTTFTSTASTSTSSTSTTQQSSSTITSSPSSTTLSTSIPTTTTPEITSTLSSLPDNAICSYLDETTTSTTFTTTMLTSTTTEEPSTSTTTTEVTSTSSTVTTTEPTTTLTTSTASTSTTEPSTSTVTTSPSTSPVTSTVTSSSSSSTTVTTPTSTESTSTSPSSTVTTSTTAPSTSTTGPSSSSSTPSSTASSSVSSTASSTQSSTSTQQSSTTTKSETTTSSDGTNPDFYFVEKATTTFYDSTSVNLTLNSGLGIIGYQTSIECTSPTSSNYVSTTKDGACFTKSVSMPRLGGTYPASTFVGPGNYTFRATMTTDDKKVYYTYANVYIQEYSSTTIESESSTSAVASSTSSTPSTPSSTLSTSTVTEPSSTRSSDSTTTSAGSTTTLQESTTTSEESTTDSSTTTISDTSTTSSSPSSTTADSTSTLSVDQFDFILDSGLSWNETRHNEDSINIVPLPTNAITPTERSQTFECRNVSTEPFLIIKESTCLNYSNTVLNATYSSNIPIQPIETFLVGIGTYEFRINMTDLTTMQVVSHIFTLNVVADSTSTSEVTSTTSTGSSSESSAISTTSGIESTSTLEASTTDASQDSSTSTSDSGTTSDSTTIDSSNSTPSTSDSSGLSQTPSDSSSASDSMRTTTVDPDASTETPYDFVLENLTWNETVYYSENPFYITPIPNKEPGALTTAMTCQCRNDSSQPFVLLKESNCLTEFGKNGAYSASVSFNPMTSFVPATGTYEFLINVTNRASGESASHIFTMNVVLPTTTTETPPTTVSSSDDAGGKTGGTGATGGTGGTGSGGSATTLSTGDAVRSTTSGSGSGQSSTGSGAGGSGTTASGSGSGGSSGTGSDGVNSGKTTALNGDGTGSGTATTPGSHLGDGGSTSGSGSDSNGSSGVSTKSSSGSDTSGSSDSSGANGAFSATAQPSTRTTKTRSSLATVSPISAAEQAIIDAQKADVMNQLAGIMDGSASNNSLNTSSSLLNQISSLPAADLVEVAQSLLSNTLKIPGVGNMSSVDVLKTLQDNIATTNSELADEMAKVITKLANVNMTSAQSLNSVLSSLDLALKGSTVYTLGVSSTKSKDGTYAVIFGYVIASGYTLVSPRCTLSIYGSTIYLTGDTRASYKQLDGDTVTADTMLAAAIGIQGMFATNGRTVQVEQDKIDDKRSLVSGNIMATMSGVGDVQSGEYSYNDMYVTAWNVTYDNSTVGSTSQKNTSFSFNIPVSEVQYILLIESGTMIKLHSTQNIVSRGLVVTASYGGVTYTITCTNGTGKFVEVDTDNAIFSYNADSFTVVASDGSSASTVKKLIQMPIVIENVNLALFNQTTSPLVFSNAGSYSMRMVLSPQDIGIPAVSALSQTVSISTLSPTASYTKDDLQSLIKEQTLVTVSGTLFFSKASSIDVSGYSFFVDSTALYLSNSVTTLVISSPTYNIVSLALGGYGIQITAGTYTSGSQTHTVTLMEFSDTQKMRIDGGLIIRNGTNGYVIQNGQVSTEGDVSGTKIDIVPQSLMNQESQQQIETILSNTQDFLTNNGMTMTDAEINDTSNSLLSIAGSLTSALKIALDNPLSSDLAANLKYATDNYDSLYNVLPSDPDNIVYVEEMTSEEWAAYVTKMFQKNIAKNLANQLASTLDTLENTLAARAIATGNLPYDYSNSVDGTGMVIVIDDASNIVGKTQNCEEWAFKLPSPASTLNTAEITDKTLIQVGLVCYATNPRTYVDNFDMLITSGALEAHIKDENQIIIPITGTTAPIYVNGRGSEDDAVLTLMQQGDFASYQILDLHAFRTTNWNNSLQVEIIASQDYEIPNNDDTYMFSSFQSLPGPLESNHEWIFDLNTLNKTSNYFVTAGNLINNTGLFFIGIGKRNSSTNTGNSSDIVNYGQYDSMQWSFARSVPMDYQVAAVSKGCYFYQKTSDVFNSEGMYPSDGQGMQFVNCSTDHLTMFSVGAFNPTIDADFSYNYNVNEIEKNVKVMIAAVFMLVVYGCLTINAIICQRKDASRGRLRFLKDNEPHDGYMYVIAVETGYRMFATTDSTICFNLSGNEGDQIFRSFRSEEDGNWEFPFSWGTTDRFVMTTAFPLGELEYMRLWLDDAGLDHRESWYCNRIIVKDLQTQDIYYFPFNNWLGTKNGDGETERLARVEYKRRFLDESMSMHMLAQTISWFAMFTGGGNRLRDRVSRQDYSVSIIFSLVVVSMISITILKSDNSIISDSKSVSEFTFTIKDIAFGVGFGVLITFLNSLHILLCTKCRSHSEHYYYKKRKREDPEFKDNSGSWPMFMAGMARTIIVFPVLMGLIYISGAGMSLMDDLANSFYIRFLISLILWAVVFEPIKGLIWAFLILKTRKSHKIINKLEEALLRAKPAETFLRNPYGKIEKGLGTEIADVTKLRDTENRKMRDEQLFITIRDMLCFFASLYIMVMLTYYCKDRHGYWYQLEMSTILNINQKNYGDNTFMSIQHADDFWDWARESLATALLASWYDGNPAYGMRAYMNDKVSRSMGIGTIRQVRTKKSAECTMFKQFQGYINDCGEELTSKNEEKTLYMQAGWTELESENGTDASDEYTYKTSEELSTETVSGLLYSYSGGGYTISMSGTQAEIITLFNKLDSERWIDDHTRAVIIEFSAYNAQINYFSVVQLLVEIPKSGIYLPNSWVESVRLIKSEGSDGTVVKYYEMLYIFFSVLIFVKEIVFYLYGRYKVITTMKPTRNPFKIVYQLALGNFSPWNFMDLIVGALAVASVLAYTIRQRTTNRAMEDFNANNGNSYINLTEQRNWEIVFSYCLAGAVFFTSCKMIRILRFNRRIGVLAATLDNALGAIVSFGIAFLFFSMTFNSVLYAVLGNKMGGYRSLMATFQTALAGMLGKLDVTSIQPISQFAFVVIMLYMIAGSKLVLQLYVTIIMFEFEEIRNDSEKQTNDYEIIDHIKYKTKRRLGLLEPKDFAPVSIADTQKDFRLFHSAVAKVNLLHHRATRMLQTQGQYQNQTVINYTLSYDPVSAIHETGPKRFQKWRLNDVEKD 1E2E5320857CED2F lov_1-o01498_1 1 3 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role bait bait c-terminus sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain physical association physical association Fig 1B. The number of amino acids in LOV-1has changed since this paper was published. The numbering for the LOV-1 C-terminal 128 amino acids have been added from the current UniProt version. hgrs_1-o01498_1 2 4 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role prey prey dsred2 fluorescent prot tag fluorescent protein tag fluorescent prot tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig 3A. In yeast, Drosophila, and mammalian cell culture, the STAM (signal-transducing adaptor molecule) and Hrs (hepatocyte growth factor regulated tyrosine kinase substrate) complex binds and sorts internalized and monoubiquitinated membrane proteins on the early endosome to the multivesicular body (MVB) for lysosomal degradation. STAM-1 and HGRS-1 expression completely overlaps in cell bodies and ciliary bases of polycystin-expressing neurons. cannot find any more GO terms - e.g. RnB dendrites, ciliary base.