IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Crosstalk between a nuclear receptor and beta-catenin signaling decides cell fates in the C. elegans somatic gonad. Crosstalk between a nuclear receptor and beta-catenin signaling decides cell fates in the C. elegans somatic gonad. Dev. Cell (1534-5807) 2006 imex curation Asahina M., Valenta T., Silhankova M., Korinek V., Jindra M. masako@paru.cas.cz, jindra@entu.cas.cz 06-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Asahina M., Valenta T., Silhankova M., Korinek V., Jindra M. masako@paru.cas.cz, jindra@entu.cas.cz Accepted 2011-MAY-19 by ORCHARD 06-Jun-2011: Contacted by JYOTI. Dev. Cell (1534-5807) 2006 orchard Only protein-protein interactions imex curation Crosstalk between a nuclear receptor and beta-catenin signaling decides cell fates in the C. elegans somatic gonad. Crosstalk between a nuclear receptor and beta-catenin signaling decides cell fates in the C. elegans somatic gonad. Dev. Cell (1534-5807) 2006 imex curation Asahina M., Valenta T., Silhankova M., Korinek V., Jindra M. masako@paru.cas.cz, jindra@entu.cas.cz 06-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Asahina M., Valenta T., Silhankova M., Korinek V., Jindra M. masako@paru.cas.cz, jindra@entu.cas.cz Accepted 2011-MAY-19 by ORCHARD 06-Jun-2011: Contacted by JYOTI. Dev. Cell (1534-5807) 2006 orchard Only protein-protein interactions imex curation Crosstalk between a nuclear receptor and beta-catenin signaling decides cell fates in the C. elegans somatic gonad. Crosstalk between a nuclear receptor and beta-catenin signaling decides cell fates in the C. elegans somatic gonad. Dev. Cell (1534-5807) 2006 imex curation Asahina M., Valenta T., Silhankova M., Korinek V., Jindra M. masako@paru.cas.cz, jindra@entu.cas.cz 06-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Asahina M., Valenta T., Silhankova M., Korinek V., Jindra M. masako@paru.cas.cz, jindra@entu.cas.cz Accepted 2011-MAY-19 by ORCHARD 06-Jun-2011: Contacted by JYOTI. Dev. Cell (1534-5807) 2006 orchard Only protein-protein interactions imex curation Crosstalk between a nuclear receptor and beta-catenin signaling decides cell fates in the C. elegans somatic gonad. Crosstalk between a nuclear receptor and beta-catenin signaling decides cell fates in the C. elegans somatic gonad. Dev. Cell (1534-5807) 2006 imex curation Asahina M., Valenta T., Silhankova M., Korinek V., Jindra M. masako@paru.cas.cz, jindra@entu.cas.cz 06-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Asahina M., Valenta T., Silhankova M., Korinek V., Jindra M. masako@paru.cas.cz, jindra@entu.cas.cz Accepted 2011-MAY-19 by ORCHARD 06-Jun-2011: Contacted by JYOTI. Dev. Cell (1534-5807) 2006 orchard Only protein-protein interactions imex curation pop1_caeel Protein pop-1 pop-1 W10C8.2 Posterior pharynx defect protein 1 protein protein caeel Caenorhabditis elegans MMADEELGDEVKVFRRDEDADDDPMISGETSEQQLADDKKEAVMEAELDGAGRNPSIDVLKSAFPKVEPMSPSFPGLMSHFSPGYSAAALPMFMPLFMNPYAAALRSPSLMFPMGAMSPTFPMFPPSPVYGAAIAAAAAKQHFENMAPLNMRAGHPMNQMGMPPYMHPSSMAPQNVDRRAQGGGKAKKDDHVKKPLNAFMWFMKENRKALLEEIGNNEKQSAELNKELGKRWHDLSKEEQAKYFEMAKKDKETHKERYPEWSARENYAVNKKKTKKRRDKSIPSENNDQKKCRARFGVNNTEMWCKFCKRKKKCEYATDRSGGSDITDSQDGRGTSGAYSSSSESPSPKANAGIALTTQQQQAAMMHTMLMQMRLGSTTGASTHVPSPLASSSAGRSPLDANASDSESDVEEEEDEQIDPTVMQQTHDMLMQESMCTI 494078BE40B40E2E nlk_caeel Serine/threonine kinase NLK nlk W06F12.1 lit-1 Nemo-like kinase Loss of intestine protein 1 protein protein caeel Caenorhabditis elegans MVSWGRGKDAYYLYISREQEEDDDDSLSFYSSQKDTEDEFCGCLFPDPEIPGSSSSSGCSSSSTELYDLAAAHAALISRQQQILSQAIPIIPEHQLAAVAAHHQHHQQLHPSVQYQLVAAATHHNHHQPQAAQPHYSAVVPRSDVIQQPPHFALHHHLQNLVQQQQQQQAHHHHQQLVGEMALVSHTHPAAVGSTTCYEKNQQKQQQVQQIPTQPQVAHVSSNAILAAAQPFYPPPVQDSQPDRPIGYGAFGVVWSVTDPRSGKRVALKKMPNVFQNLASCKRVFREIKMLSSFRHDNVLSLLDILQPANPSFFQELYVLTELMQSDLHKIIVSPQALTPDHVKVFVYQILRGLKYLHTANILHRDIKPGNLLVNSNCILKICDFGLARTWDQRDRLNMTHEVVTQYYRAPELLMGARRYTGAVDIWSVGCIFAELLQRKILFQAAGPIEQLQMIIDLLGTPSQEAMKYACEGAKNHVLRAGLRAPDTQRLYKIASPDDKNHEAVDLLQKLLHFDPDKRISVEEALQHRYLEEGRLRFHSCMCSCCYTKPNMPSRLFAQDLDPRHESPFDPKWEKDMSRLSMFELREKMYQFVMDRPALYGVALCINPQSAAYKNFASSSVAQASELPPSPQAW A9F2EA7E3E2CE7F5 nhr25_caeel Nuclear hormone receptor family member nhr-25 nhr-25 F11C1.6 protein protein caeel Caenorhabditis elegans MTDVERMVLRPNHEGEMCPVCGDRVSGYHYGLLTCESCKGFFKRTVQNKKQYQCSAEANCHVDRTCRKRCPSCRFQKCLTMGMKMEAVRADRMRGGRNKFGSFYKKDRAHRMQRNAMRVSTVQVPAVLGAQSQAQTFYQPPEHQVSSSTTDQNNQINYFDQTKIKTEYIKTEYDAHLQSPTLSSSTNQQLSVSDFIMRPGYLVDPQDSLAVLLGSTIDDPLLRHTFPAAYQLNEVKQEPFDYSEQFIHHSLHDYPTYTSNTTNYATMMPMTTVSSTQSLVTSTSSTTTGRMTEASSTSPILPLCPAPTEKTVDHFYNSSIAEMCKTLPDDAQIARIFTSVKGTSKPEKHAFSIQVAEENLKDIVIWAKNDQLFSKLSLDDQMILLQTSWTTVHIVDITNAMVHGNLLSQYKMSNGDEVPVGLVALLGNQTFVSSWNDVVIRLRNMGFTNFDYCAFRFLALFDQSMDSFPAVSTARSRVLQSWREVRCTTAFLEIFEQIRRLAYDSLRYLWNLHSNCPTNWEQFFPEASLVLEMIRTTVNRSASSSVTAITQVPAIQLPTPQATYTAVPYMAS wrm1_caeel Armadillo repeat-containing protein wrm-1 wrm-1 Worm armadillo protein 1 B0336.1 protein protein caeel Caenorhabditis elegans MDVDCAETFSQPCTPLNFNPMTPSTSRVSTPVRPSSTMSARQYSGSPFKAQPQNMEPSNSRVQELREAAVGKRSYTNAWMQGTYAPPQMAGQQQRFSRPPSVIGSTMSHMTNMSEMTAYSYGGLSMLSVNTEMGEFNNFVNQAPYQRALTRVSQVSENQDPTNRQYPMTAPEIIENLESTELINQAAAIRALEPIVKAGGMLQTWGPKGAEPIIRALFQVLIPRPVENENVIRKAFEILHHSILLSNKEIRRIDRMFFRLNAALMDPNGPPVFNVPKPYSIYEIVMTRAIQLDTKFESSAMVLLVHLCCKPHFMKIFFGEDETQQSPAHRRLHKIVIEFAIGNLRRPETKSKNKGLCVSIIKNLSNKNATIKDMSERLGVVSLFHQIMQNEVIHEDLLWSTMQALTVFCGDVKNGTHFVQMGGAQVLCGLLSHGSTRLLHELLKCLRRVSDLPAIQEQDMKESIHCIVQLIGCSDVTIVELATGTLRNIGLHNKMNKAFMVQDGVTSHAIAVLRTSEQFTYQPHANIDLYRKQILSIYENCLSVLNNVTSMAPQDIKESAVSACRMISENADSAYVLLHYFNVGNRKCRKLAVTVMKRVIETVPAFADPFVDLLGTTNEPLPILLLQRAFQSLDEWRKTSVEMMNCDGRSAEQRRELDDRRKDHEDIVKRSVGLLTNLCSQANPRFFHSLKLVLTNGTLNPFQWLTHEMSDGILQEWLAFILSICSRDESLQTFMMYRFLEQAKMTEAFFAELKARRQNSNIQTMLSKIIDLGRHQQRIVSQQHQQHQMQHHRQLM B517C7A804A7DEA8 q9xvi2_caeel sys-1 T23D8.9 CELE_T23D8.9 protein protein caeel Caenorhabditis elegans MHSTGEPQRGPAGPYHHPMPYHVDQIPTTSHPSWHQAPHPGLPTPPYHPQQHPNYISMQPPPSQQQQMTPQALSTQQQQQVQQQQQRQLYSSPSPSRGPAAPAQNQKFRTEQWINNQAWPYSQSPAPPVAPSVMSYHQGDDRMSMLSVNTTMTNQFPDSQRCYSSNGSTCENVNPEMMQMNITQAAEQAIRLWFNTPDPMQRLHMAKTIRTWIRQDKFAQVDQANMPNCVQQILNIIYDGLKPQPVQLPISYYAQLWYNLLDILRRFTFLPIISPYIHQVVQMFCPRENGPQDFRELICNLISLNWQKDPHMKHCANQVFQIFNCIIMGVKNEKLRTEFAQHLKFEKLVGTLSEYFNPQVHPGMINPAIFIIFRFIISKDTRLKDYFIWNNNPHDQPPPPTGLIIKLNAVMIGSYRLIAGQNPETLPQNPELAHLIQVIIRTFDLLGLLLHDSDAIDGFVRSDGVGAITTVVQYPNNDLIRAGCKLLLQVSDAKALAKTPLENILPFLLRLIEIHPDDEVIYSGTGFLSNVVAHKQHVKDIAIRSNAIFLLHTIISKYPRLDELTDAPKRNRVCEIICNCLRTLNNFLMMWIPTPNGETKTAGPNEKQQVCKFIEIDILKKLMSCLSCEGMDTPGLLELRSTILRSFILLLRTPFVPKDGVLNVIDENRKENLIGHICAAYSWVFRQPNNTRTQSTKQQLVERTISLLLVLMEQCGAEKEVAQYSYSIDCPLNLLNGNQVKPTFIHNVLVVCDKILEHCPTRADIWTIDRPMLEGLTNHRNSDIAKAANSLLSRFPENDLLAGIFSNREYF wrm1-nhr25 4 7 unspecified role unspecified role prey prey region-minus dna binding domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role bait bait minus_c_terminal 23 amino_acids sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 4A lit_1-wrm_1-1 4 8 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association pop_1-sys_1-1 4 5 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association sys_1-nhr-25 3 9 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey 35s-met 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association Fig 4B wrm_1-nhr25 3 7 unspecified role unspecified role prey prey 35s-met 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 8 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig 4B nhr25-wrm_1 2 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 4C nhr25-q9xvi2 1 7 unspecified role unspecified role bait bait region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined delta- ligand binding domain required to bind necessary binding region required to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 4D