IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ RNA-binding proteins SOP-2 and SOR-1 form a novel PcG-like complex in C. elegans. RNA-binding proteins SOP-2 and SOR-1 form a novel PcG-like complex in C. elegans. Development (0950-1991) 2006 imex curation Zhang T., Sun Y., Tian E., Deng H., Zhang Y., Luo X., Cai Q., Wang H., Chai J., Zhang H. zhanghong@nibs.ac.cn 06-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions ecoli Escherichia coli (strain K12) pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Zhang T., Sun Y., Tian E., Deng H., Zhang Y., Luo X., Cai Q., Wang H., Chai J., Zhang H. Proteins were purified by the GST affinity column followed by ion exchange and Superdex columns. zhanghong@nibs.ac.cn Accepted 2011-MAY-20 by ORCHARD 06-Jun-2011: Contacted by JYOTI. Development (0950-1991) 2006 orchard Only protein-protein interactions imex curation RNA-binding proteins SOP-2 and SOR-1 form a novel PcG-like complex in C. elegans. RNA-binding proteins SOP-2 and SOR-1 form a novel PcG-like complex in C. elegans. Development (0950-1991) 2006 imex curation Zhang T., Sun Y., Tian E., Deng H., Zhang Y., Luo X., Cai Q., Wang H., Chai J., Zhang H. zhanghong@nibs.ac.cn 06-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions caeel-embryo Caenorhabditis elegans embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Zhang T., Sun Y., Tian E., Deng H., Zhang Y., Luo X., Cai Q., Wang H., Chai J., Zhang H. Untagged protein identified by immunostaining zhanghong@nibs.ac.cn Accepted 2011-MAY-20 by ORCHARD 06-Jun-2011: Contacted by JYOTI. Development (0950-1991) 2006 orchard Only protein-protein interactions imex curation RNA-binding proteins SOP-2 and SOR-1 form a novel PcG-like complex in C. elegans. RNA-binding proteins SOP-2 and SOR-1 form a novel PcG-like complex in C. elegans. Development (0950-1991) 2006 imex curation Zhang T., Sun Y., Tian E., Deng H., Zhang Y., Luo X., Cai Q., Wang H., Chai J., Zhang H. zhanghong@nibs.ac.cn 06-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa predetermined predetermined participant predetermined Zhang T., Sun Y., Tian E., Deng H., Zhang Y., Luo X., Cai Q., Wang H., Chai J., Zhang H. zhanghong@nibs.ac.cn Accepted 2011-MAY-20 by ORCHARD 06-Jun-2011: Contacted by JYOTI. Development (0950-1991) 2006 orchard Only protein-protein interactions imex curation RNA-binding proteins SOP-2 and SOR-1 form a novel PcG-like complex in C. elegans. RNA-binding proteins SOP-2 and SOR-1 form a novel PcG-like complex in C. elegans. Development (0950-1991) 2006 imex curation Zhang T., Sun Y., Tian E., Deng H., Zhang Y., Luo X., Cai Q., Wang H., Chai J., Zhang H. zhanghong@nibs.ac.cn 06-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Zhang T., Sun Y., Tian E., Deng H., Zhang Y., Luo X., Cai Q., Wang H., Chai J., Zhang H. zhanghong@nibs.ac.cn Accepted 2011-MAY-20 by ORCHARD 06-Jun-2011: Contacted by JYOTI. Development (0950-1991) 2006 orchard Only protein-protein interactions imex curation sop2_caeel Polycomb protein sop-2 C50E10.4 sop-2 Suppressor of pal-1 protein 2 protein protein caeel Caenorhabditis elegans MSSNLTSNEMSSTSAIVEPPEAVKGNERDKSTRRSTSQVVRPEKHEVENALEDQCSSSSLPKEAQYYAKLDKKLEGKDPRSQFYEAVRLSADIFAHKFEKAVCSRQTFEPTNSIIKVLNTAEEEMLHEKVVPLPVSSKLQYYLNRGRYDTIFDRDEQLQRTADPMADEPDHVEKRVTSILEQASREMEEGEVEPVFYDGSDEDQELPIDLGAMRNLQRTNKFAARSSRMAARRGGRPGYRGAFRGAARGAPSRRPAPAAEVAPETPVAAPMAPAAPAAPATPEAAPAAEVMDTSIATEMPQESAVDLSNVSAATEMDTSKEGEASRPTSEKKKKIRTTEMDRLMSMDLGPKDGGRVGELGHMWPESRRRPAAPLPETPAAQPRKSLPRRAAEKKKPEDSDAAEEQEVEMEVDNDASTSTPRNARGGRGGGNRRGSRRGQKRTSGGSGKLVEPKKEPVDEPAEKIPKRSEAAPEVPATATTKEAPPSTSSSPPDAPATPATPASSDSRDSPRKIRAMIFSLTGSPPESETPPVLQQEQVISTAAPTAGRHPNIIQQVPHINRIPPQPLRRLTAPQAPPASQPEEPPVQQTVPVVKVELASAPAPIVRDPQSTEPVPPAMPTLVENNHEATLILPPNKTSDYTRWNAQDLINWVRLLITNNVDSTIAIMVREEFDGETLACLVLDDDFRKEVPIPYGHYKKMKIYGTEVLNHYRTEKYQADLRKFHEELAAWKAQQR BE95913E04329519 egl-5_5utr_rna rna ribonucleic acid RNA rna chemical synthesis Chemical synthesis (Chemical synthesis) 5′UTR of egl-5, C08C3 nucleotides 41280-41840) sor1_caeel Sop-2-related protein 1 ZK1236.3 sor-1 protein protein caeel Caenorhabditis elegans MTINIKYSSKFSSSKTSSSEELKPKTYIPAYYQPPVSMPKYYVNWLRIKLSLNKLKNIRAIYLFDQCQNFNQYQESSRKFSAGQVSSLTPWYSNFSNYSTVILRMKDTSLPPLENPSNGGTYLFNLITVFPSIALSFNYSWRGDTGPSISIFSFSLSVLFFSLFPQHKNICARAWCRPFRRSLSFSLRFIMSSEPASSSTEKVPEEPHPHSIKHKFQGPQFVIPRALSDYVLNVNNQTPESYEKALNAKYGRDDYITLCLHVTSLCTEYGPLDDGPEYVLLCDSRARVDKLIEDLVKLLEIDTDYVQLELHGGKRLHLQKPDAVLRDIAYKQSNEGSDKFFLEMKLVPSESMKAKIMKQEEEEEKARKHGQYQQYQEYHQQHQAMNDGQSSSSVPSTSSPSCSSEANRKEMETVREPAGPSELMRAINAPVAPAPVVIKIETPVALPEEDETLMDDDEMPSLTVEAPSEEASFEAEQPSPQVPQASIEGPSQQQQIPGTSQQKRQVARGSRTNMISYHDLPPGTGNAPPMACPQVTLKLEKNVPFEAKIRAVAGYTRKPISEVQKMRPSDLESIFHSICIASVQRIKRRNELVQQLQEINAQSCKSPTMTMNKKFTLAKAYQRVQNEIEKIDREQILPQQYMNMPPMPPQGQQRLPPPAYPPGILPPQQNRQQGVPPQFQRSPQFMIGPDGQRYAHPYMQLPNSNQRARILNTSSVQPSEEVRNRLVKIEAMAMNMAQLNPPRPPPPQPPHRALQGELQFLRPGAPDPCNFRPDSKQTYNNTYVTVASPATLTNSIIPWHFPPYEKSGRLNVSNTIKAINEYRLLCNSRQADPASFLEFYFLGDPMPHFNKILSIADYNMYLSRRRCDEADVKIHRMSHSDQLQLYLLELQSDESNVEKWKTFYRIMQWDLPLNNEFPRILLPSSLDIGRPVVDRKKKSIDQVMNHIHRMHSQRPPSMGNSSTSSEASSTSPTNAATATSSPASNRPTTSTAQPPTLNPT D230C9A6F84928E2 egl_5-sor_1 3 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait region radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Fig 3C sop_2-sor_1-1 2 7 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig 4E sop_2-sor_1-3 4 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 21 23 direct interaction direct interaction Fig 5A sor_1-sop_2 4 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 27 30 direct interaction direct interaction Fig 5B sop_2-sor_1-5 1 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 35 37 physical association physical association Fig 5D