UniProt UniProt http://www.uniprot.org Transactivation of Schizosaccharomyces pombe cdt2+ stimulates a Pcu4-Ddb1-CSN ubiquitin ligase. Transactivation of Schizosaccharomyces pombe cdt2+ stimulates a Pcu4-Ddb1-CSN ubiquitin ligase. EMBO J. (0261-4189) 2005 imex curation Liu C., Poitelea M., Watson A., Yoshida SH., Shimoda C., Holmberg C., Nielsen O., Carr AM. a.m.carr@sussex.ac.uk orchard Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column anti tag western anti tag western blot anti tag western Liu C., Poitelea M., Watson A., Yoshida SH., Shimoda C., Holmberg C., Nielsen O., Carr AM. a.m.carr@sussex.ac.uk Accepted 2011-MAR-04 by ORCHARD EMBO J. (0261-4189) 2005 orchard Only protein-protein interactions imex curation Transactivation of Schizosaccharomyces pombe cdt2+ stimulates a Pcu4-Ddb1-CSN ubiquitin ligase. Transactivation of Schizosaccharomyces pombe cdt2+ stimulates a Pcu4-Ddb1-CSN ubiquitin ligase. EMBO J. (0261-4189) 2005 imex curation Liu C., Poitelea M., Watson A., Yoshida SH., Shimoda C., Holmberg C., Nielsen O., Carr AM. a.m.carr@sussex.ac.uk orchard Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Liu C., Poitelea M., Watson A., Yoshida SH., Shimoda C., Holmberg C., Nielsen O., Carr AM. a.m.carr@sussex.ac.uk Accepted 2011-MAR-04 by ORCHARD EMBO J. (0261-4189) 2005 orchard Only protein-protein interactions imex curation Transactivation of Schizosaccharomyces pombe cdt2+ stimulates a Pcu4-Ddb1-CSN ubiquitin ligase. Transactivation of Schizosaccharomyces pombe cdt2+ stimulates a Pcu4-Ddb1-CSN ubiquitin ligase. EMBO J. (0261-4189) 2005 imex curation Liu C., Poitelea M., Watson A., Yoshida SH., Shimoda C., Holmberg C., Nielsen O., Carr AM. a.m.carr@sussex.ac.uk orchard Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast tap tandem affinity purification TAP TAP tap fingerprinting peptide massfingerprinting fingerprinting Liu C., Poitelea M., Watson A., Yoshida SH., Shimoda C., Holmberg C., Nielsen O., Carr AM. a.m.carr@sussex.ac.uk Accepted 2011-MAR-04 by ORCHARD EMBO J. (0261-4189) 2005 orchard Only protein-protein interactions imex curation tcpz_schpo T-complex protein 1 subunit zeta cct6 CCT-zeta SPBC646.11 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MLSLLNPKAESIQRAQALQVNISAAIGLQDVLKSNLGPTGTTKMLVDGAGAIKLTKDGKVLLTEMQIQNPTASCIAKAATAQDDATGDGTTSVCLLVGELLKQAELYIREGLHPSLISDGFNLAKNEALTFLDSFKTDFEVDREVLLNVAKTSLSTKISSKVVESLAPAVVDAILTIRRPDEPIDLHMVEIMKMQNRSASDTQLIRGLLLDHGARHPDMPKQVKNAYILILNVSLEYEKSEINSGFFYSTSEQRERLVESERKFVDNKLRKIVELKKEVCERDPTANFVIINQKGIDPLSLDVLAKNGIMALRRAKRRNMERLQLACGGVAQNSVDDLNPEVLGWAGSVYERTLGEEKYTFVEDVKDPKSATILIHGPNTYTIQQIQDATRDGLRAVKNAVEDNCLIVGAGAFEVACAAHLRNKFAAKEVKGKAKMGVYAYADALLIIPKTLAANSSYDTQDAIVALQEEASEGYKVGLDLKTGMPFDPEVEGIYDNYRVIRHMLHSATVIASNLISVDQILRAGRSSLKEGPPQ 9FABB5CA3CAF511E csn1_schpo COP9 signalosome complex subunit 1 csn1 SPBC215.03c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSLNLLVNQEELKRYLDEYGVWSKIFRALFVARNSKPLRSFCVHYAIKELKEKTYNLELYQSLFEEFQDCFENEQLDVEWVESVTFHRKQNLEQLRRELKAYKNNLIRESIRAAQLDLASFFADVGQFDSALRSYAKVREYCTNAGQIAHLSLELMRISIWIGNYSHVLAFGSRAKSTVSAAMELTSPIYAYCGLANFCLGDYEEALAHFLKVETDTSDGIITKTDISVYISLCALACWDHKRLIQELERNEEFNALSDLDPSLRRCLQAKCNRKYSLLLDTLQQNAQDYSLDMYLAPQLTNLFSLIRERSLLDYLIPYSALPFSKIAVDFHIDENFIEKNLLEIIEAKKLNGKVDSLQKRVYIEPSSEPENFEDIKNIAQTSLLYSKALYLQTLAAMNTENTDDEAPVIAQTNITAEDSQN 37E701F90CEDB2C2 tcph_schpo Probable T-complex protein 1 subunit eta cct7 CCT-eta SPBC25H2.12c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSLGGPQIPVIVLKEGTDDSQGRGQLLSNINACVAVQDTIRTTLGPLGADKLMVDDRGEVVISNDGATIMKLLDIVHPAAKTLVDIARAQDAEVGDGTTSVVVFAGELLREARTFVEDGVSSHLIIRGYRKAAQLAVNKIKEIAIHLDLSDEGKLRDLLTKCASTAMNSKLIRSNSTFFTKMVVDAVLTLDQEDLNENMIGIKKVPGGAMEDSLLVKGVAFKKTFSYAGFEQQPKFFKNPKILCLDVELELKAEKDNAEVRVDKVQEYQNIVDAEWRIIFSKLEAIVATGAKVVLSKLPIGDLATQYFADRDIFCAGRVAADDLNRVVQAVGGSIQSTCSNIEEKHLGTCDTFEERQIGGDRFNLFEGCPKAKTCTLILRGGADQFIAEVERSLHDAIMIVKHALKNNLVVAGGGACEMELSKYLRDYSLTISGKQQNFIAAFARSLEVIPRQLCDNAGFDSTNILNKLRMQHAKGEMWAGVDMDSEGVANNFEKFVWEPSTVKSNAILSATEAATLILSVDETIKNEPSQQPQAPQGALPPGAANRIMRGRGRGMPR C4C1F843F44C5253 cdt2_schpo Cell division cycle protein cdt2 cdt2 SPAC17H9.19c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MNMDIGKALSDVGGLRDITSRANNSLPPTPDSSPAAPSKKHKLYDFLESRGKIETPRKRIVFEKKPLLHKPVHIQKKPAQLCKELLIRQLGGSRSHPFSTKTSRHVGGRLNLETYYSRPSECLMMLNQLPFCLGFANNESLLAVCTETGALELFDSRFYDRQNEENQPSARRIHGWLAHNNAIFSVNFSKDDSLLATSSGDQTSKVFDLSTQQCITRLGRRGVDGYHSHSVKQVNFCNDSPYNLVSCSRDGSIIFWDMRTHGITIDGEHFQKPVLRIRKAHENSGRDCSITSATWLPQSTSQVISSCSANSALKLWDLRTVHTVRPLPAATTPELTTSKRDFGVTNVCTSPDGERIYAASRDSIIYEYSSRHLNSGFCKTYKDPRLRISSFYVKLACSPDGATLACGGGVQDKTSGVVVFDTTRNCSSSAMLTGGHTKDVTAVDWSSEGQLASISDDGSVRVWNSSLHGSAANLREKNFSEIFYWGFSEK 4BB352E3AC3110E6 ddb1_schpo DNA damage-binding protein 1 ddb1 Damage-specific DNA-binding protein 1 SPAC17H9.10c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MTYVTYLHKPSSIRNAVFCKFVNASSWNVIVAKVNCLEVYSYENNRLCLITSANIFAKIVNVKAFKPVSSPTDHIIVATDSFRYFTLFWDANDNTVSNGIKIQDCSERSLRESQSGPLLLVDPFQRVICLHVYQGLLTIIPIFKSKKRFMTSHNNPSLHDNFSVRIQELNVVDIAMLYNSSRPSLAVLYKDSKSIVHLSTYKINVREQEIDEDDVVCHDIEEGKLIPSENGGVFVFGEMYVYYISKDIQVSKLLLTYPITAFSPSISNDPETGLDSSIYIVADESGMLYKFKALFTDETVSMELEKLGESSIASCLIALPDNHLFVGSHFNNSVLLQLPSITKNNHKLEILQNFVNIAPISDFIIDDDQTGSSIITCSGAYKDGTLRIIRNSINIENVALIEMEGIKDFFSVSFRANYDNYIFLSLICETRAIIVSPEGVFSANHDLSCEESTIFVSTIYGNSQILQITTKEIRLFDGKKLHSWISPMSITCGSSFADNVCVAVAGGLILFFEGITEVGRYQCDTEVSSLCFTEENVVYVGLWSADIIMLTYCQDGISLTHSLKLTDIPRSIVYSQKYGDDGGTLYVSTNNGYVLMFNFQNGQVIEHSLRRNQLGVAPIILKHFDSKEKNAIFALGEKPQLMYYESDKLVITPLSCTEMLNISSYVNPSLGVNMLYCTNSYISLAKMSEIRSLNVQTVSVKGFPRRICSNSLFYFVLCMQLEESIGTQEQRLLSFLRVYEKNTLSEIAHHKFNEYEMVESIILMNDDKRVVVGTGFNFPDQDAPDSGRLMVFEMTSDNNIEMQAEHKVQGSVNTLVLYKHLIVAGINASVCIFEYEHGTMHVRNSIRTPTYTIDISVNQDEIIAADLMKSITVLQFIDDQLIEVARDYHPLWATSVEILSERKYFVTEADGNAVILLRDNVSPQLSDRKKLRWYKKFYLGELINKTRHCTFIEPQDKSLVTPQLLCATVDGSLMIVGDAGMSNTPLLLQLQDNIRKVIPSFGGLSHKEWKEYRGENETSPSDLIDGSLIESILGLREPILNEIVNGGHEGTKLDISVQDLKSIIENLEKLHP 70AB463DE14351F3 csn4_schpo COP9 signalosome complex subunit 4 csn4 SPAC22A12.03c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MEEVVHYFLEGNMPVAQFREALALHYTNEKELFEQAKRCLNICCGSNNFAKRNDVLFSLLDVAVSISSLELRKELISELYVPVQSLEEAPSEYLVSCCLQLATIYEAEQNFELLCSSLEAVEKHGHFENDLEQLLLLRIRLGDAYLKLGKAEKAILTVRTSIPLAFKVSNDQLLMELQLCNARALDETGQFLEAAKCYYRVLQYKVPGNELIYRENLCSVAQCLLLAIPSPIVLQFLQEISLQPSVREIPFYSLVEKYLKRKFIGKEDGAFLLPFLLPHQVIHMNRLIEDGRHFLETNILFLSEFFEVSSTSILAKHFKLSEEQVDTVVADMVIQERLNASIDQCEGYITFLPEYGKANNLPNYVNKIATVLQHYGS 49087FF0DAACC53E tcpd_schpo T-complex protein 1 subunit delta cct4 CCT-delta SPBC106.06 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSKAATVPVAFQDREKPQEVRLSNIMAARSVADAIRTSLGPKGMDKMIQTGKGEVILTNDGATILKHLSVLHPAAKMLVDLSAAQDVEAGDGTTSVVILAGSMLACAEKLLKKGIHPTVIAESFQRAAGFTVDCMKENALAIELSDRESLLRAATTSLNSKIVSQYSNLLAPIAVDAVLKVIDPRVATNVDLKDIRIVKKLGGIIDDTELIPGLALTQTAVKSAGGPTRIEKANIALIQFQLSPPKPDMENQVVVNDYRQMDKILKEERQYLLNMCKKIKKAGANVILIQKSILRDAVNDLALHFLAKLKIMVIKDIERDEVEFICKSTGCKPIADIESFAEDKLGHADLVEETSSSGEKIVKFSGVKNAGKTVSILCRGANLLTLEEAERSLHDALCVIRCLVKQRALIAGGGSPEIEAAQRLLEHARQLEGREAICIRAFSEALEIIPVTLAENAGLNAIQVVTELRSRHANGEKTAGINVRKGIVTNILEENVLQPLLVNISAIQLAAETTKMIMKIDDITLAR ACB5D86F9A83C02E tcpa_schpo T-complex protein 1 subunit alpha cct1 CCT-alpha SPBC12D12.03 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MFQAPRESTLFLSGEKISGEDVRNQNVLATTAIANVVKSSLGPVGLDKMLVDDIGDVTVTNDGATILSLLDVEHPAGKVLVELAQQQDKEVGDGTTSVVIIAAELLRRANELVKNKIHPTTIITGYRLAIREAVKFMTDVLSCSVDSLGKESLINVAKTSMSSKIIGNDSDFFSTMAVDAMLSVKTSNSKGETRYPVKAVNILKAHGKSSRESVLVKGYALNCTIASQAMKTRVQNAKIAVLDMDLQKTKMALGVHVTIDDPDQLEKIREREVMITLERVKKILNAGANVILTTKGIDDLCLKSIIEAGAMAVRRCKKEDLRRIAKASGATLLSSLSNLEGEETFESSYLGSAEEVVQEKFSDDECILVKGTKAYSSASIVLRGPNEYSLDEMERSMHDSLSVVKRTLESGKVVPGGGAVETALSIYLENFATSLGSREQLAIAEFAQALLIIPRTLAVNAAKDSTELTAKLRAYHAASQNAEVTDVKKRGYKNYGLDLLNGVIRDNVKAGVLEPSMSKLKSLKSAVEACIAILRIDTSIKLDPERQPEDPHAGLH 34DE8D22AA56D812 cul4_schpo Cullin-4 pcu4 cul4 SPAC3A11.08 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MPPEAKRIVVKGFDPRKSRQRQETYYVTMIDRLNMALQVVMAGLGLKTGYQELYSGVENLTRADQASRCFNILQHHMSSGIQLLKDSAESFIQLEGTETDTNACTVVVGCWNKWLERVEIVQNIFYYMDKTFLSHHPDYPTIEELSLSLFREKLMAVKNIQIPFLNSLLQSFENLHSSKSTDHAYLQDAMLMLHRTEMYSSVFVPMYLVMLSRFYDTESSQKIQELPLEEYLEYAMSSLEREDAYVEKFDIVRDKKSIRETVQRCLITSHLDTLTKGISQFIEKRDAHSCKLLYALLQFNHETEYLIQPWSDCLVDVGFKLVNDESKDDTLVQELLSFHKFLQVVVDESFLHDETLSYAMRKAFETFINGAKGSQREAPARLIAKYIDYLLRVGEQASGGKPLKEVFSEILDLFRYIASKDIFEAYYKLDIAKRLLLNKSASAQNELMLLDMLKKTCGSQFTHSLEGMFRDVNISKEFTSSFRHSKAAHNLHRDLYVNVLSQAYWPSYPESHIRLPDDMQQDLDCFEKFYLSKQVGKKISWYASLGHCIVKARFPLGNKELSISLFQACVLLQFNNCLGGEGISYQDLKKSTELSDIDLTRTLQSLSCARIRPLVMVPKSKKPSPDTMFYVNEKFTDKLYRVKINQIYLKEERQENSDVQEQVVRDRQFELQASIVRVMKQKEKMKHDDLVQYVINNVKDRGIPLVSDVKTAIEKLLEKEYLEREDNDIYTYVT E90FFFE06D071C3E csn2_schpo COP9 signalosome complex subunit 2 csn2 SPAPB17E12.04c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSNDFMLEDDENYDFEFEDDDDDMIEPYVDVENCYYNSKSLKEENPESALTSFYSIVEKCEGEQNEWAFKALKQITKINFQLKKYDDMLQSYQRLLGYTNWLSITKNYSEKSIYNIVEYASSCENTEFLEKFYDVTTKALQNLNNERLMLKVLMHVARFLLTQKNYHKFKYLLRQMHELLSDENNSVADQNRGTHLLELYSLEIQMYSDIEDNKRLKELYQSSLRVKTAIPHPRIMGIIRECGGKMHMQENQWSEAQTNFFESFKSYDEAGSSDRIRVLKYLVLANMLSESEINPFDSPETQPYKDNPHIIAMTKLVEAYQIRDITAVERILQTYQHDILDDDFIRQYVDKILYSIRSQVLIELVKPYTSVKLSLLAKKLGVSISIIEQALVGLIIDERVNGKIDMVNEVFTISQPKNTIHNQLVEDVQKLWNTATK D36C501FB43B52FA csn1-cdt2 2 5 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1A This interaction is csn2 and ddb1 dependent. ddb1-cdt2 2 8 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1B csn1-ddb1 2 5 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1C csn2-ddb1-cdt2 1 8 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 2A These proteins fractionate at a peak between 400 and 800 kDa cdt2-csn2 1 8 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 2A These proteins fractionate at a peak between 200 kDa cdt2-cdt1 3 5 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 2B