MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Identification of major Ca(2+)/calmodulin-dependent protein kinase phosphatase-binding proteins in brain: biochemical analysis of the interaction. Identification of major Ca(2+)/calmodulin-dependent protein kinase phosphatase-binding proteins in brain: biochemical analysis of the interaction. Arch. Biochem. Biophys. (0003-9861) 2005 Ishida A., Tada Y., Nimura T., Sueyoshi N., Katoh T., Takeuchi M., Fujisawa H., Taniguchi T., Kameshita I. suggested by santonico aishida@asahikawa-med.ac.jp partial coverage rapid curation in vitro In vitro phosphatase assay phosphatase assay autoradiography autoradiography Ishida A., Tada Y., Nimura T., Sueyoshi N., Katoh T., Takeuchi M., Fujisawa H., Taniguchi T., Kameshita I. suggested by santonico aishida@asahikawa-med.ac.jp Arch. Biochem. Biophys. (0003-9861) 2005 partial coverage rapid curation Identification of major Ca(2+)/calmodulin-dependent protein kinase phosphatase-binding proteins in brain: biochemical analysis of the interaction. Identification of major Ca(2+)/calmodulin-dependent protein kinase phosphatase-binding proteins in brain: biochemical analysis of the interaction. Arch. Biochem. Biophys. (0003-9861) 2005 Ishida A., Tada Y., Nimura T., Sueyoshi N., Katoh T., Takeuchi M., Fujisawa H., Taniguchi T., Kameshita I. suggested by santonico aishida@asahikawa-med.ac.jp partial coverage rapid curation rat-brain Rattus norvegicus brain brain Brain [cerebrum] far western blotting far western blotting Affinity blotting Affinity blotting fingerprinting peptide massfingerprinting fingerprinting Ishida A., Tada Y., Nimura T., Sueyoshi N., Katoh T., Takeuchi M., Fujisawa H., Taniguchi T., Kameshita I. suggested by santonico aishida@asahikawa-med.ac.jp Arch. Biochem. Biophys. (0003-9861) 2005 partial coverage rapid curation Identification of major Ca(2+)/calmodulin-dependent protein kinase phosphatase-binding proteins in brain: biochemical analysis of the interaction. Identification of major Ca(2+)/calmodulin-dependent protein kinase phosphatase-binding proteins in brain: biochemical analysis of the interaction. Arch. Biochem. Biophys. (0003-9861) 2005 Ishida A., Tada Y., Nimura T., Sueyoshi N., Katoh T., Takeuchi M., Fujisawa H., Taniguchi T., Kameshita I. suggested by santonico aishida@asahikawa-med.ac.jp partial coverage rapid curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Ishida A., Tada Y., Nimura T., Sueyoshi N., Katoh T., Takeuchi M., Fujisawa H., Taniguchi T., Kameshita I. suggested by santonico aishida@asahikawa-med.ac.jp Arch. Biochem. Biophys. (0003-9861) 2005 partial coverage rapid curation Identification of major Ca(2+)/calmodulin-dependent protein kinase phosphatase-binding proteins in brain: biochemical analysis of the interaction. Identification of major Ca(2+)/calmodulin-dependent protein kinase phosphatase-binding proteins in brain: biochemical analysis of the interaction. Arch. Biochem. Biophys. (0003-9861) 2005 Ishida A., Tada Y., Nimura T., Sueyoshi N., Katoh T., Takeuchi M., Fujisawa H., Taniguchi T., Kameshita I. suggested by santonico aishida@asahikawa-med.ac.jp partial coverage rapid curation in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps tag fluorescence tag visualisation by fluorescence tag fluorescence Ishida A., Tada Y., Nimura T., Sueyoshi N., Katoh T., Takeuchi M., Fujisawa H., Taniguchi T., Kameshita I. suggested by santonico aishida@asahikawa-med.ac.jp Arch. Biochem. Biophys. (0003-9861) 2005 partial coverage rapid curation Identification of major Ca(2+)/calmodulin-dependent protein kinase phosphatase-binding proteins in brain: biochemical analysis of the interaction. Identification of major Ca(2+)/calmodulin-dependent protein kinase phosphatase-binding proteins in brain: biochemical analysis of the interaction. Arch. Biochem. Biophys. (0003-9861) 2005 Ishida A., Tada Y., Nimura T., Sueyoshi N., Katoh T., Takeuchi M., Fujisawa H., Taniguchi T., Kameshita I. suggested by santonico aishida@asahikawa-med.ac.jp partial coverage rapid curation rat-brain Rattus norvegicus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip monoclonal western monoclonal antibody western blot monoclonal western Ishida A., Tada Y., Nimura T., Sueyoshi N., Katoh T., Takeuchi M., Fujisawa H., Taniguchi T., Kameshita I. suggested by santonico aishida@asahikawa-med.ac.jp Arch. Biochem. Biophys. (0003-9861) 2005 partial coverage rapid curation aldoa_rat Fructose-bisphosphate aldolase A Muscle-type aldolase Aldoa protein protein rat Rattus norvegicus (Rat) Rat MPHPYPALTPEQKKELADIAHRIVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYRQLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKSKGGVVGIKVDKGVVPLAGTNGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSSLAIMENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHVYLEGTLLKPNMVTPGHACTQKFSNEEIAMATVTALRRTVPPAVPGVTFLSGGQSEEEASINLNAINKCPLLKPWALTFSYGRALQASALKAWGGKKENLKAAQEEYIKRALANSLACQGKYTPSGQSGAAASESLFISNHAY 2774497C20DCC0DA kcc4_mouse Calcium/calmodulin-dependent protein kinase type IV Camk4 CaM kinase-GR protein protein mouse Mus musculus Mouse MLKVTVPSCPSSPCSSVTASTENLVPDYWIDGSNRDPLGDFFEVESELGRGATSIVYRCKQKGTQKPYALKVLKKTVDKKIVRTEIGVLLRLSHPNIIKLKEIFETPTEISLVLELVTGGELFDRIVEKGYYSERDARDAVKQILEAVAYLHENGIVHRDLKPENLLYATPAPDAPLKIADFGLSKIVEHQVLMKTVCGTPGYCAPEILRGCAYGPEVDMWSVGIITYILLCGFEPFYDERGDQFMFRRILNCEYYFISPWWDEVSLNAKDLVKKLIVLDPKKRLTTFQALQHPWVTGKAANFVHMDTAQKKLQEFNARRKLKAAVKAVVASSRLGSASSSHTSIQENHKASSDPPSTQDAKDSTDLLGKKMQEEDQEEDQVEAEASADEMRKLQSEEVEKDAGVKEEETSSMVPQDPEDELETDDPEMKRDSEEKLKSVEEEMDPMTEEEAPDAGLGVPQQDAIQPEY mint Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory CE1F98670822F975 g3p_rat Glyceraldehyde-3-phosphate dehydrogenase Gapdh 38 kDa BFA-dependent ADP-ribosylation substrate BARS-38 Gapd Peptidyl-cysteine S-nitrosylase GAPDH protein protein rat Rattus norvegicus (Rat) Rat MVKVGVNGFGRIGRLVTRAAFSCDKVDIVAINDPFIDLNYMVYMFQYDSTHGKFNGTVKAENGKLVINGKPITIFQERDPANIKWGDAGAEYVVESTGVFTTMEKAGAHLKGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIVSNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDDIKKVVKQAAEGPLKGILGYTEDQVVSCDFNSNSHSSTFDAGAGIALNDNFVKLISWYDNEYGYSNRVVDLMAYMASKE D9E5ED4F38544C77 kcc1a_rat Calcium/calmodulin-dependent protein kinase type 1 Camk1 CaM kinase I CaM kinase I alpha protein protein rat Rattus norvegicus (Rat) Rat MPGAVEGPRWKQAEDIRDIYDFRDVLGTGAFSEVILAEDKRTQKLVAIKCIAKKALEGKEGSMENEIAVLHKIKHPNIVALDDIYESGGHLYLIMQLVSGGELFDRIVEKGFYTERDASRLIFQVLDAVKYLHDLGIVHRDLKPENLLYYSLDEDSKIMISDFGLSKMEDPGSVLSTACGTPGYVAPEVLAQKPYSKAVDCWSIGVIAYILLCGYPPFYDENDAKLFEQILKAEYEFDSPYWDDISDSAKDFIRHLMEKDPEKRFTCEQALQHPWIAGDTALDKNIHQSVSEQIKKNFAKSKWKQAFNATAVVRHMRKLQLGTSQEGQGQTASHGELLTPTAGGPAAGCCCRDCCVEPGSELPPAPPPSSRAMD Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes like transcriptional regulation, hormone production, translational regulation, regulation of actin filament organization and neurite outgrowth. Involved in calcium-dependent activation of the ERK pathway (By similarity). Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CTFR, MYL9, SYN1/synapsin I and SYNII/synapsin II mint 37889B3DEF033AB2 ppm1e_rat Protein phosphatase 1E Ppm1e Camkn Ca(2+)/calmodulin-dependent protein kinase phosphatase N CaMKP-nucleus Partner of PIX 1 Partner of PIX-alpha protein protein rat Rattus norvegicus (Rat) Rat MAGCIPEEKTYRRFLELFLGEFRGPCGGGEPEPEPESEPEPEPEAELVAAEAAEASGEDPGEDAATVEAAEEGVQDQDPEPEEEAVEEEAAAEGEEEEEEEEAAAPGHSAVPPPQPQLPPLPPLPRPLSERITREEVEGESLDLCLQQLYKYNCPSFLAAALARATSDEVLQSDLSAHCIPKETDGTEGTVEIETVKLARSVFSKLHEICCNWVKDFPLRRRPQIYYETSIHAIKNMRRKMEDKHVCIPDFNMLFNLEDQEEQAYFAVFDGHGGVDAAIYASVHLHVNLVRQEMFPHDPAEALCRAFRVTDERFVQKAARESLRCGTTGVVTFIRGNMLHVAWVGDSQVMLVRKGQAVELMKPHKPDREDEKQRIEALGGCVVWFGAWRVNGSLSVSRAIGDAEHKPYICGDADSASTVLDGTEDYLILACDGFYDTVNPDEAVKVVSDHLKENNGDSSMVAHKLVASARDAGSSDNITVIVVFLRDMNKAVNVSEESDWTENSFQGGQEDGGDDKENHGECKRPWPQHQCSAPADLGYEGRVDSFTDRTSLSPGPQINVLEDPDYLDLTQIETSKPHSTQFLPPVEMIGPGAPKKAYVNELIMEESSVTPSQPERSGAGELLVSFNLGSTGQQICRMENLSPVYSGLENEQFKSPGKRASRLYHLRHHYSKRQRGFRFNPKFYSFFSAQESSRKIGISLSSLTRSGKRNKMLRSSLPWRENSWEGYSGNMAIRKRNNISCPDLPWDYKI mint Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2. Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42. Inactivates multifunctional CaM kinases AD18B60517D57347 aldoa-ppm1e 2 10 enzyme enzyme neutral component neutral component 6 enzyme target enzyme target substrate substrate neutral component neutral component physical association physical association mint F1; T1 gapdh-ppm1e 2 10 enzyme enzyme neutral component neutral component 8 enzyme target enzyme target substrate substrate neutral component neutral component physical association physical association mint F1; T1;F2 gapdh-ppm1e-1 4 10 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component direct interaction direct interaction F3 mint aldoa-ppm1e-1 4 10 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component direct interaction direct interaction F3 mint ppm1e-gapdh 5 10 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association F4 mint ppm1e-gapdh-1 3 10 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association F5 mint gapdh-ppm1e-2 3 10 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association F5 mint camk1-ppm1e 1 9 enzyme target enzyme target substrate substrate neutral component neutral component 10 enzyme enzyme neutral component neutral component dephosphorylation dephosphorylation reaction dephosphorylation F9 mint camk4-ppm1e 1 10 enzyme enzyme neutral component neutral component 7 enzyme target enzyme target substrate substrate neutral component neutral component dephosphorylation dephosphorylation reaction dephosphorylation F7 mint