UniProt UniProt http://www.uniprot.org Human PTIP facilitates ATM-mediated activation of p53 and promotes cellular resistance to ionizing radiation. Human PTIP facilitates ATM-mediated activation of p53 and promotes cellular resistance to ionizing radiation. J. Biol. Chem. (0021-9258) 2004 mimix curation Jowsey PA., Doherty AJ., Rouse J. 1. anti bait coip fig 2B: have no source/host for the exp 2. cannot interprete immunofluoresc exp fig 2C and 3E as colocalization Origin of HA-tagged 54BP1 as human according pmid:9748285 (fig 2A) j.rouse@dundee.ac.uk 23-Sept-2011: Contacted by JYOTI. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Jowsey PA., Doherty AJ., Rouse J. 1. anti bait coip fig 2B: have no source/host for the exp 2. cannot interprete immunofluoresc exp fig 2C and 3E as colocalization Origin of HA-tagged 54BP1 as human according pmid:9748285 (fig 2A) j.rouse@dundee.ac.uk Accepted 2011-SEP-01 by ORCHARD 23-Sept-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2004 mimix curation Human PTIP facilitates ATM-mediated activation of p53 and promotes cellular resistance to ionizing radiation. Human PTIP facilitates ATM-mediated activation of p53 and promotes cellular resistance to ionizing radiation. J. Biol. Chem. (0021-9258) 2004 mimix curation Jowsey PA., Doherty AJ., Rouse J. 1. anti bait coip fig 2B: have no source/host for the exp 2. cannot interprete immunofluoresc exp fig 2C and 3E as colocalization Origin of HA-tagged 54BP1 as human according pmid:9748285 (fig 2A) j.rouse@dundee.ac.uk 23-Sept-2011: Contacted by JYOTI. human-at22ije-tpebs7-yz5 Homo sapiens at22ije-tpebs7-yz5 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Jowsey PA., Doherty AJ., Rouse J. 1. anti bait coip fig 2B: have no source/host for the exp 2. cannot interprete immunofluoresc exp fig 2C and 3E as colocalization Origin of HA-tagged 54BP1 as human according pmid:9748285 (fig 2A) j.rouse@dundee.ac.uk Accepted 2011-SEP-01 by ORCHARD 23-Sept-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2004 mimix curation Human PTIP facilitates ATM-mediated activation of p53 and promotes cellular resistance to ionizing radiation. Human PTIP facilitates ATM-mediated activation of p53 and promotes cellular resistance to ionizing radiation. J. Biol. Chem. (0021-9258) 2004 mimix curation Jowsey PA., Doherty AJ., Rouse J. 1. anti bait coip fig 2B: have no source/host for the exp 2. cannot interprete immunofluoresc exp fig 2C and 3E as colocalization Origin of HA-tagged 54BP1 as human according pmid:9748285 (fig 2A) j.rouse@dundee.ac.uk 23-Sept-2011: Contacted by JYOTI. human-at22ije-tpebs7 Homo sapiens A-T fibroblasts with empty vector at22ije-tpebs7 A-T fibroblasts with empty vector anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Jowsey PA., Doherty AJ., Rouse J. 1. anti bait coip fig 2B: have no source/host for the exp 2. cannot interprete immunofluoresc exp fig 2C and 3E as colocalization Origin of HA-tagged 54BP1 as human according pmid:9748285 (fig 2A) j.rouse@dundee.ac.uk Accepted 2011-SEP-01 by ORCHARD 23-Sept-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2004 mimix curation Human PTIP facilitates ATM-mediated activation of p53 and promotes cellular resistance to ionizing radiation. Human PTIP facilitates ATM-mediated activation of p53 and promotes cellular resistance to ionizing radiation. J. Biol. Chem. (0021-9258) 2004 mimix curation Jowsey PA., Doherty AJ., Rouse J. 1. anti bait coip fig 2B: have no source/host for the exp 2. cannot interprete immunofluoresc exp fig 2C and 3E as colocalization Origin of HA-tagged 54BP1 as human according pmid:9748285 (fig 2A) j.rouse@dundee.ac.uk 23-Sept-2011: Contacted by JYOTI. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Jowsey PA., Doherty AJ., Rouse J. 1. anti bait coip fig 2B: have no source/host for the exp 2. cannot interprete immunofluoresc exp fig 2C and 3E as colocalization Origin of HA-tagged 54BP1 as human according pmid:9748285 (fig 2A) j.rouse@dundee.ac.uk Accepted 2011-SEP-01 by ORCHARD 23-Sept-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2004 mimix curation paxi1_human PAX-interacting protein 1 PAX transactivation activation domain-interacting protein PAXIP1 PAXIP1L PTIP CAGF28 protein protein human Homo sapiens Human MSDQAPKVPEEMFREVKYYAVGDIDPQVIQLLKAGKAKEVSYNALASHIISEDGDNPEVGEAREVFDLPVVKPSWVILSVQCGTLLPVNGFSPESCQIFFGITACLSQVSSEDRSALWALVTFYGGDCQLTLNKKCTHLIVPEPKGEKYECALKRASIKIVTPDWVLDCVSEKTKKDEAFYHPRLIIYEEEEEEEEEEEEVENEEQDSQNEGSTDEKSSPASSQEGSPSGDQQFSPKSNTEKSKGELMFDDSSDSSPEKQERNLNWTPAEVPQLAAAKRRLPQGKEPGLINLCANVPPVPGNILPPEVRGNLMAAGQNLQSSERSEMIATWSPAVRTLRNITNNADIQQMNRPSNVAHILQTLSAPTKNLEQQVNHSQQGHTNANAVLFSQVKVTPETHMLQQQQQAQQQQQQHPVLHLQPQQIMQLQQQQQQQISQQPYPQQPPHPFSQQQQQQQQAHPHQFSQQQLQFPQQQLHPPQQLHRPQQQLQPFQQQHALQQQFHQLQQHQLQQQQLAQLQQQHSLLQQQQQQQIQQQQLQRMHQQQQQQQMQSQTAPHLSQTSQALQHQVPPQQPPQQQQQQQPPPSPQQHQLFGHDPAVEIPEEGFLLGCVFAIADYPEQMSDKQLLATWKRIIQAHGGTVDPTFTSRCTHLLCESQVSSAYAQAIRERKRCVTAHWLNTVLKKKKMVPPHRALHFPVAFPPGGKPCSQHIISVTGFVDSDRDDLKLMAYLAGAKYTGYLCRSNTVLICKEPTGLKYEKAKEWRIPCVNAQWLGDILLGNFEALRQIQYSRYTAFSLQDPFAPTQHLVLNLLDAWRVPLKVSAELLMSIRLPPKLKQNEVANVQPSSKRARIEDVPPPTKKLTPELTPFVLFTGFEPVQVQQYIKKLYILGGEVAESAQKCTHLIASKVTRTVKFLTAISVVKHIVTPEWLEECFRCQKFIDEQNYILRDAEAEVLFSFSLEESLKRAHVSPLFKAKYFYITPGICPSLSTMKAIVECAGGKVLSKQPSFRKLMEHKQNSSLSEIILISCENDLHLCREYFARGIDVHNAEFVLTGVLTQTLDYESYKFN DIP protein Q6ZW49 original sequence version: 47 DIP protein Q6ZW49 original sequence version: 48 78CD535A150E1FC1 tp53b_human TP53-binding protein 1 TP53BP1 protein protein human Homo sapiens Human MDPTGSQLDSDFSQQDTPCLIIEDSQPESQVLEDDSGSHFSMLSRHLPNLQTHKENPVLDVVSNPEQTAGEERGDGNSGFNEHLKENKVADPVDSSNLDTCGSISQVIEQLPQPNRTSSVLGMSVESAPAVEEEKGEELEQKEKEKEEDTSGNTTHSLGAEDTASSQLGFGVLELSQSQDVEENTVPYEVDKEQLQSVTTNSGYTRLSDVDANTAIKHEEQSNEDIPIAEQSSKDIPVTAQPSKDVHVVKEQNPPPARSEDMPFSPKASVAAMEAKEQLSAQELMESGLQIQKSPEPEVLSTQEDLFDQSNKTVSSDGCSTPSREEGGCSLASTPATTLHLLQLSGQRSLVQDSLSTNSSDLVAPSPDAFRSTPFIVPSSPTEQEGRQDKPMDTSVLSEEGGEPFQKKLQSGEPVELENPPLLPESTVSPQASTPISQSTPVFPPGSLPIPSQPQFSHDIFIPSPSLEEQSNDGKKDGDMHSSSLTVECSKTSEIEPKNSPEDLGLSLTGDSCKLMLSTSEYSQSPKMESLSSHRIDEDGENTQIEDTEPMSPVLNSKFVPAENDSILMNPAQDGEVQLSQNDDKTKGDDTDTRDDISILATGCKGREETVAEDVCIDLTCDSGSQAVPSPATRSEALSSVLDQEEAMEIKEHHPEEGSSGSEVEEIPETPCESQGEELKEENMESVPLHLSLTETQSQGLCLQKEMPKKECSEAMEVETSVISIDSPQKLAILDQELEHKEQEAWEEATSEDSSVVIVDVKEPSPRVDVSCEPLEGVEKCSDSQSWEDIAPEIEPCAENRLDTKEEKSVEYEGDLKSGTAETEPVEQDSSQPSLPLVRADDPLRLDQELQQPQTQEKTSNSLTEDSKMANAKQLSSDAEAQKLGKPSAHASQSFCESSSETPFHFTLPKEGDIIPPLTGATPPLIGHLKLEPKRHSTPIGISNYPESTIATSDVMSESMVETHDPILGSGKGDSGAAPDVDDKLCLRMKLVSPETEASEESLQFNLEKPATGERKNGSTAVAESVASPQKTMSVLSCICEARQENEARSEDPPTTPIRGNLLHFPSSQGEEEKEKLEGDHTIRQSQQPMKPISPVKDPVSPASQKMVIQGPSSPQGEAMVTDVLEDQKEGRSTNKENPSKALIERPSQNNIGIQTMECSLRVPETVSAATQTIKNVCEQGTSTVDQNFGKQDATVQTERGSGEKPVSAPGDDTESLHSQGEEEFDMPQPPHGHVLHRHMRTIREVRTLVTRVITDVYYVDGTEVERKVTEETEEPIVECQECETEVSPSQTGGSSGDLGDISSFSSKASSLHRTSSGTSLSAMHSSGSSGKGAGPLRGKTSGTEPADFALPSSRGGPGKLSPRKGVSQTGTPVCEEDGDAGLGIRQGGKAPVTPRGRGRRGRPPSRTTGTRETAVPGPLGIEDISPNLSPDDKSFSRVVPRVPDSTRRTDVGAGALRRSDSPEIPFQAAAGPSDGLDASSPGNSFVGLRVVAKWSSNGYFYSGKITRDVGAGKYKLLFDDGYECDVLGKDILLCDPIPLDTEVTALSEDEYFSAGVVKGHRKESGELYYSIEKEGQRKWYKRMAVILSLEQGNRLREQYGLGPYEAVTPLTKAADISLDNLVEGKRKRRSNVSSPATPTASSSSSTTPTRKITESPRASMGVLSGKRKLITSEEERSPAKRGRKSATVKPGAVGAGEFVSPCESGDNTGEPSALEEQRGPLPLNKTLFLGYAFLLTMATTSDKLASRSKLPDGPTGSSEEEEEFLEIPPFNKQYTESQLRAGAGYILEDFNEAQCNTAYQCLLIADQHCRTRKYFLCLASGIPCVSHVWVHDSCHANQLQNYRNYLLPAGYSLEEQRILDWQPRENPFQNLKVLLVSDQQQNFLELWSEILMTGGAASVKQHHSSAHNKDIALGVFDVVVTDPSCPASVLKCAEALQLPVVSQEWVIQCLIVGERIGFKQHPKYKHDYVSH DIP protein Q12888 original sequence version: 117 DIP protein Q12888 original sequence version: 110 DIP protein Q12888 original sequence version: 113 DIP protein Q12888 original sequence version: 165 DIP protein Q12888 original sequence version: 149 DIP protein Q12888 original sequence version: 143 13E2CC8A265F9D2A paxip1-tp53bp1 1 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association 1d Ionizing radiation (20 Gy) paxip1-tp53bp1-1 2 5 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2a the interaction is enhanced upon IR-stimulation; the enhancement requires serine-protein kinase ATM which phosporylates TP53BP1. paxip1-tp53bp1-3 3 5 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2a the interaction is enhanced upon IR-stimulation; the enhancement requires serine-protein kinase ATM which phosphorylates TP53BP1. paxip1-tp53bp1-4 4 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association 3A and B