UniProt UniProt http://www.uniprot.org BTB/POZ domain proteins are putative substrate adaptors for cullin 3 ubiquitin ligases. BTB/POZ domain proteins are putative substrate adaptors for cullin 3 ubiquitin ligases. Mol. Cell (1097-2765) 2003 imex curation Geyer R., Wee S., Anderson S., Yates J., Wolf DA. dwolf@hsph.harvard.edu 06-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip polyclonal western polyclonal antibody western blot polyclonal western Geyer R., Wee S., Anderson S., Yates J., Wolf DA. dwolf@hsph.harvard.edu Accepted 2011-APR-19 by ORCHARD 06-Jun-2011: Contacted by JYOTI. Mol. Cell (1097-2765) 2003 orchard Only protein-protein interactions imex curation BTB/POZ domain proteins are putative substrate adaptors for cullin 3 ubiquitin ligases. BTB/POZ domain proteins are putative substrate adaptors for cullin 3 ubiquitin ligases. Mol. Cell (1097-2765) 2003 imex curation Geyer R., Wee S., Anderson S., Yates J., Wolf DA. dwolf@hsph.harvard.edu 06-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast pull down pull down anti tag western anti tag western blot anti tag western Geyer R., Wee S., Anderson S., Yates J., Wolf DA. dwolf@hsph.harvard.edu Accepted 2011-APR-19 by ORCHARD 06-Jun-2011: Contacted by JYOTI. Mol. Cell (1097-2765) 2003 orchard Only protein-protein interactions imex curation BTB/POZ domain proteins are putative substrate adaptors for cullin 3 ubiquitin ligases. BTB/POZ domain proteins are putative substrate adaptors for cullin 3 ubiquitin ligases. Mol. Cell (1097-2765) 2003 imex curation Geyer R., Wee S., Anderson S., Yates J., Wolf DA. dwolf@hsph.harvard.edu 06-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast pull down pull down polyclonal western polyclonal antibody western blot polyclonal western Geyer R., Wee S., Anderson S., Yates J., Wolf DA. dwolf@hsph.harvard.edu Accepted 2011-APR-19 by ORCHARD 06-Jun-2011: Contacted by JYOTI. Mol. Cell (1097-2765) 2003 orchard Only protein-protein interactions imex curation BTB/POZ domain proteins are putative substrate adaptors for cullin 3 ubiquitin ligases. BTB/POZ domain proteins are putative substrate adaptors for cullin 3 ubiquitin ligases. Mol. Cell (1097-2765) 2003 imex curation Geyer R., Wee S., Anderson S., Yates J., Wolf DA. dwolf@hsph.harvard.edu 06-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast pull down pull down mudpit multidimensional protein identification technology mudpit Geyer R., Wee S., Anderson S., Yates J., Wolf DA. dwolf@hsph.harvard.edu Accepted 2011-APR-19 by ORCHARD 06-Jun-2011: Contacted by JYOTI. Mol. Cell (1097-2765) 2003 orchard Only protein-protein interactions imex curation ubc4_schpo Ubiquitin-conjugating enzyme E2 4 ubc4 Ubiquitin carrier protein 4 Ubiquitin-protein ligase 4 SPBC119.02 E2 ubiquitin-conjugating enzyme 4 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MALKRINRELADLGKDPPSSCSAGPVGDDLFHWQATIMGPADSPYAGGVFFLSIHFPTDYPFKPPKVNFTTRIYHPNINSNGSICLDILRDQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHVYKTDRSRYELSAREWTRKYAI 3E8BADFF494A91EB btb1_schpo BTB/POZ domain-containing protein 1 btb1 SPCC330.11 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSHLLFAYYLCNDIRSFQNLLKQDDSKVKEPRKGFSEKSGQKLRINQKDRYGRTVLHIAVSENKNSFVRSLLQHKGIDVFVQDEESGYTALHRAIYVGNLEAASLLLSKDPSFRSLRIKDKEGLSPFQFLSRVLSSTIHPVLDLPIIGNELYGFGTNVNNTLGIANGKEPSSPERVFLLKNQTESPTSGQLFSRDKILDVQASKFHSVVLTDEPSQNVYVCGIGAGGRIGFNTDVQYNFIPIPGIIHKVIQISVSHTHSLALTKFGSIYSWGKNGSGELGLSNDELKKDDPIQITPRRISAFKDYQIIGMAAGKSYSVAWTDTDIYSWGLNNGQLGISDHISVVSTPRRVAGLLSPVIHAVCTTRATICLLQNNSIIAFCNYNQVKLPFNVDFGSSLKVTKHPLSLTRFNVRKLLASENKLAVLTELGEVYEFDMKLLLDRDSTSSKNSTRTSFKFTPLWIFESSDLAALDIAWTADNSLILCTRNGTCWKRELRSKKREKSSSSPYSRGPYKYNRIENLQMVVGVRASASGSFFAIRNDYLPPPIYKPSNMLIDLLRSLLPYDHLLHVRQPRLIPPEDEDGVPIFDEDRAASSNEMQLLFEGSIPILTSYENYKQSFSDVTIYCGTSMFHSHKFILCARSSFLRKLLLQKKKSSVSNIIYIEEITQSHSTIRVEDIPPLAVAILLHYLYTDTLLSPWHLDSRFSPLKENLSKLANLLELPHLAEVLPFSVSRQPLLSLTNDILQLYNNFYVLCEETMDTVIKLKDGELKAHGLFLSLRSEYFSSYFQFVSMESNSFDIPITVNLSHLTVEHMSIVLRHVYSDLKVELFDDLKESDFHNWLETMFEILSIADELLFLELKSIAQQSLLRFLNLKTLPTLMDLSLSYHAEELYSRCIDYACHNIEFFLEANRISEWDGFHLKKVAQRLTELLSDQRVHLPSSKIANRLLIRDPVLMEKRNYELKVLREYLFSQESSQLWDDSPYRSIFEDRRCSTSAVILESGIVPSSNQSDSLNKEDAEEKSPKPNVVNVTSITKTAGASVEIQNNIESASSGGDKTQLNGPGADQPVTATITFDKTSPWRNRENLSHNNNTTRASLRELLEQEKADASTTTVLSDSRFMKAPTKKSQREKKKELSKQVPISKTNVGHIDIELGKSNHSNPWSVATHQRGSFSSSTGVKKSFNGILREAAREEGSSQVIYQESKKRISNGSPTSWNLLTKPSPRSASLPKNSQPLSISEIMTEQKEEIESQKRRSSFRKTIEEIQQEEEFQKWWEEESLRVQKELGILKTERDTSTNRKQGQASKQPQRRHRKEKDSKVSESTAEFKSLPIDIPRTTHKKGKARAVK A5A3C9FF2E682C3C rbx1_schpo RING-box protein pip1 pip1 SPAC23H4.18c Pop-interacting protein 1 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MEDEMQIDKKEVEIEQKPPRFEIKKWNAVALWQWDIVVDNCAICRNHIMDLCIECQANTDSAAAQECTVAWGTCNHAFHFHCISRWLNTRNVCPLDNREWEFQRYGH 71C3A3D2BF26DABF cul3_schpo Cullin-3 cul3 pcu3 SPAC24H6.03 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MQRSAKLKIRAPRKFSANQVDFATHWEVLQRAIGDIFQKSTSQLSFEELYRNAYILVLHKYGEKLYNHVQDVIRSRLKEETVPAIYKNYDASLLGNALLDIRKNDSYSTSWSRSLEAAHRFLSSLVNSWKDHIVSMQMISSVLKYLDKVYSKSADKVPVNENGIYIFREVVLLNSFEIGEKCVETILILVYLERKGNTINRPLINDCLDMLNSLPSENKKETLYDVLFAPKFLSYTRNFYEIESSTVIGVFGVVEYLKKAEKRFEEEKERSKNYLFTKIASPLLSVVEDELLSKHLDDLLENQSTGFFSMIDSSNFEGLQLVYESFSRVELGVKSLKKYLAKYVAHHGKLINETTSQALEGKMAVGRLSSNATMATLWVQKVLALWDRLNTIISTTMDADRSILNSLSDAFVTFVDGYTRAPEYISLFIDDNLKKDARKAIEGSIEATLQNSVTLFRFISEKDVFEKYYKTHLAKRLLNNRSISSDAELGMISRLKQEAGNVFTQKLEGMFNDMNLSQELLQEYKHNSALQSAKPALDLNVSILASTFWPIDLSPHKIKCNFPKVLLAQIDQFTDFYLSKHTGRKLLWYPSMGSADVRVNFKDRKYDLNVSTIASVILLLFQDLKENQCLIFEEILEKTNIEVGDLKRNLQSLACAKYKILLKDPKGREVNAGDKFYFNENFVSNLARIKISTVAQTRVEDDSERKRTLEKVDESRKHQADACIVRVMKDRKVCEHNQLMAEVTRQLNPRFHPSPMMIKRRIEALIEREYLQRQADNGRIYEYLA 0830F7A857DF6CE7 tcpd_schpo T-complex protein 1 subunit delta cct4 CCT-delta SPBC106.06 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSKAATVPVAFQDREKPQEVRLSNIMAARSVADAIRTSLGPKGMDKMIQTGKGEVILTNDGATILKHLSVLHPAAKMLVDLSAAQDVEAGDGTTSVVILAGSMLACAEKLLKKGIHPTVIAESFQRAAGFTVDCMKENALAIELSDRESLLRAATTSLNSKIVSQYSNLLAPIAVDAVLKVIDPRVATNVDLKDIRIVKKLGGIIDDTELIPGLALTQTAVKSAGGPTRIEKANIALIQFQLSPPKPDMENQVVVNDYRQMDKILKEERQYLLNMCKKIKKAGANVILIQKSILRDAVNDLALHFLAKLKIMVIKDIERDEVEFICKSTGCKPIADIESFAEDKLGHADLVEETSSSGEKIVKFSGVKNAGKTVSILCRGANLLTLEEAERSLHDALCVIRCLVKQRALIAGGGSPEIEAAQRLLEHARQLEGREAICIRAFSEALEIIPVTLAENAGLNAIQVVTELRSRHANGEKTAGINVRKGIVTNILEENVLQPLLVNISAIQLAAETTKMIMKIDDITLAR ACB5D86F9A83C02E btb3_schpo BTB/POZ domain-containing protein 3 btb3 SPAC13D6.04c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MASQDQNTGTTHVELQGGENSKKLFSKYDLWSKAMDEKKLSSSLFTVNDTQEFLELCEACRRGDLEVVKSLVENYNTPINQVDQFDYSPLVLASLCGHEPVVKFLLENGALCERDTFQGERCLYGALNDNIRRMLLSYDITKAIDESQPYASHITSLLSNSALHFTTDIVFAGQYGRVFAHKFYLAARSSYFKSKFSKLGPSEHEIEVKHFAKEFESILRYLYLDTNAVFTKQYNNALLSIGKKFQLNDFIALYEKDREQLHSRDWKKIQLAKTQNDLGEFLDYIISNYKVPIESLNQPSDQYSFHDAYLQSYTHRYPVHRAIMCRCEYFLDMLAGPFLESNQELPVLSLPFSSSVVEIVLKFLYTDKTDIAPELALDVVYVADMLSLDKDRSLKSLASIVITKQEEPIDSIYDILRTAWDTSTPRLEQYASEYMANHLEHLIDDPEFCELVKESADRILQRQETDTIELIDDIRYFLSKRFGIYHEDLCIDGVVDTLTPYESEYNQKMEMIDDLLDKLELQA B565690ECCBF6A5E ubl1_schpo Ubiquitin-like protein 1 ubl1 ned8 SPBC12D12.08c SPBC24C6.01c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MLIKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYHLEGGSVLHLVLALRGGSC 57656CEBDB824234 prs7_schpo 26S proteasome regulatory subunit 7 homolog rpt1 SPBC16C6.07c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MPPKEDWEKYQKPVDTEEENDKNPPPLDEGDIELLKSYATGPYARELAAIKEDTEAVLKRINDTVGIKESDTGLAPISFWDVAADRQRMSEEQPLQVARCTKIIENEQSAEKNAYVINLKQIAKFVVSLGERVSPTDIEEGMRVGCDRNKYAIQLPLPPKIDPSVTMMQVEEKPDVTYGDVGGCKEQIERLREVVELPLLSPERFVKLGIDPPKGIMLYGPPGTGKTLCARAVANRTDATFIRVIGSELVQKYVGEGARMVRELFEMARTKKACIIFFDEIDAIGGARFDDGAGGDNEVQRTMLELITQLDGFDPRGNIKVLFATNRPNTLDEALMRPGRIDRKVEFGLPDLEGRAHILRIHAKSMAIDKDIRWELIARLCPSQTGAELRSVCTEAGMFAIRARRRVATEKDFLDAVQKVVKGNQKFSSTADYMNMSS D1D5D24C926FC74D rpn2_schpo 26S proteasome regulatory subunit rpn2 rpn2 SPBC17D11.07c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MTMVYSDNTSIITSAGGLMALLDEQERELQVHALLKIYEFIDQLWPEISDDVTKIEVMYEDHSFPERELAALVVSKVYYYLGEYDEALLFALSSGPKFLHDKNSDYKETLIFKCIDMFIHKSAELYKNPKADPLDERLSGVVEGIFQKCYAKNEWRHVLGIAIEAHRLDIIEYILNADKETDLKPYVLELAMTVVLDIEFRNRLLRLLLSSFLTETEPDYFSVGKCVVHLNDASVAAKLLMKLSSQNDDKSLLTAYQLAFDLEDSAPQEFLNSVMDLLPSPSVANSEEDANADSKKEDSSPCGYIIRILSGEQTVKYDREFLYAHNNTDMLILNRTKDSLEARNSVFHNAVTFANAFMNFGTSSDSFFRDNLSWLSKANNWSKFTATAALGVIHRGYYNQAMNILRPYLPEEDAPSSSTYSEGGAFYAMGLIHANHGRGVTEYLREQLKHTEDEIVQYGLLLGIGLTGMASRDETLYESVKTILFNDNAVAGSAAGISMGLIMLGTASSAAIDEMLQYAHETQHEKIIRGLGIGIALIVYGRQQEADGIIKELNNDLDPTLRYAGMFATALAYCGTSNSKIVRDVLHISVSDVNDDVRRAAVCALGFICFKDPNALISTVELLVDSYNPHVRYGSAIALGIACANSGSNAALDLLSRLVEDATDFVRQGAMIAQAMILTQHNDQLNSKVSGIRKHFEQVINEKHEDALAKLGATLAQGIIDAGGRNVTIALQTATGSLKLSAIVGLTVFLQYWYWFPLTHFMSLSFSPTALIGLDKNLNAPKFSFISNVRPKLFAYPPKSVQPTAKTVQKVETAVLSTTVKAQARAKRAEREKASKGSNDDEMKIDKKTTEEKEATPMEMDEEKSQDISINGNSKKEEPKSETLENFTRVVPAQLPYISFNLNGRYYPVRKFTGGVLMLIDRESDKAPDLIELNRDAVPASADTEPGEQEASPPEDFEYPFDDDD E8D1758722127F31 prs4_schpo 26S proteasome regulatory subunit 4 homolog mts2 Protein mts2 SPBC4.07c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MGQAQSGNFSNFGDGANGDNKKDQKKDKPKYEPPVPTRTGRRKKKAQSGPDASAKLPTVIPTTRCRLRLLKMQRIHDHLLMEEEYVQNQERLKPQDERTQEERNRVDEIRGTPMSVGTLEEIIDDDHAIVSTAGPEYYVSIMSFVDKDMLEPGCSVLLHHKAMSIVGLLLDDTDPMINVMKLDKAPTESYADIGGLESQIQEIKEAVELPLTHPELYEEMGIKPPKGVILYGAPGTGKTLLAKAVANQTSATFLRVVGSELIQKYLGDGPRLVRQLFNAAEEHSPSIVFIDEIDAIGTKRYDAQSGAEREIQRTMLELLNQLDGFDTSQRDIKVIMATNRISDLDPALIRPGRIDRKILFENPDEATKRKIFTIHTSKMNLGEDVNLEELIQCKDDLSGAEIKAIVSEAGLLALRERRMRVVMDDFRQAREKVLKTKDEGGPAGGLYI 451AEEF6A24C29D4 prs8_schpo 26S proteasome regulatory subunit 8 homolog let1 Protein let1 SPBC23G7.12c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MTEVLKTNVLQSNENIVQYYTQKIQDAELAILQKTQNLRRLEAQRNGLNARVRLLREEIQLLQEPGSYVGEVIKTMGKNKVLVKVHPEGKYVVDISPDIDIKEIKPNIRVALRNDSYQLIKILPNKVDPLVSLMMVEKIPDSTYEMVGGLEKQIKEIKEVIELPVKHPELFESLGIPQPKGILLYGPPGTGKTLLARAVAHHTDCKFIRVSGSELVQKYIGEGSRMVRELFVMAREHAPSIIFMDEIDSIGSSRSDSSGGSGDSEVQRTMLELLNQLDGFEATKNIKVIMATNRIDILDPALLRPGRIDRKIEFPPPSAEARAEILRIHSRSMNLTRGIDLKSIAEKMNGASGAELKGVCTEAGMFALRERRVHVTQEDFELAVAKVLNKGDSGEMSLQKLFK 36DEF12C39841315 adt_schpo ADP,ATP carrier protein anc1 ADP/ATP translocase Adenine nucleotide translocator SPBC530.10c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MATSSAAAAASTPVNANTITETKNSTFFFDFMMGGVSAAVSKTAAAPIERVKLLIQNQDEMIRAGRLSHRYKGIGECFKRTAAEEGVISLWRGNTANVLRYFPTQALNFAFKDKFKKMFGYKKERDGYAKWFAGNLASGGAAGAASLLFVYSLDYARTRLANDAKSAKKGGERQFNGLVDVYRKTYRSDGLRGLYRGFGPSVVGIVVYRGLYFGMYDTLKPVVLVGPLEGNFLASFLLGWAVTTGSGVASYPLDTIRRRMMMTSGEAVKYSSSFECGRQILAKEGARSFFKGAGANILRGVAGAGVLSIYDQVQLLMFGKKF 8AC3D16A40F41AFC tcpq_schpo Probable T-complex protein 1 subunit theta cct8 CCT-theta SPBC337.05c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MALRVPKASGPQLFREGYRIMQGVEDAVIRNCNAIRELSEITRTSLGPNGKNKIVVNHLQQTFLTNDAATIIRELEVIHPAAKLVVDATQQQENELGDAANFVVVFTGELLAKAENMIRMGLTPLEIAKGYEMALSHTMEVLEEICADKIETVESEKELIKAIRTCISSKQYGNEDFLSDLVAKAILTVLPKDPSKFNVDNIRVVKIMGSSLYNSQVVKGMVFPREPEGTVTRSKEAKVAVFSCPLDISQTETKGTVLLHNAQEMLDFSKGEENLIESHIKEIYDAGVRVVVTSGNVNDLVLHYLNRFEILVIRVPSKFELRRLCRVVGATPLARMGVPMPEEMGSVDVVETIEIGGDRVTVFRQVEDITRTATIVLRGATKTYLDDLERAIDDGVNIVKALVKDNRLIFGAGASDMQLCIRLISVGEKTPGIYQHAIKQYGEAFEVVPRTISENAGLDPTDVISKLYAAHHKENGESIGVDVECENDGTLDAKEAGIFDVLLAKKSAIRLATETVLTVLNVDQVVMSKPAGGPKPPGPNPHWDDD 61EEBF8CE3A4ACEE btb2_schpo BTB/POZ domain-containing protein 2 btb2 SPBC25B2.06c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSMNSSTEISQEPSIMKPVSSISSFVFNAGFLQGTFSDTTLIIKGETYHLHALFLGRSPVLLQKLIENNPKGDVHYTIEVETEDPYVTKESCLFVLSTLYCDSPRIPAEVNVCSVLAVSDLLGLDTLAFEASSLIEKSIRPETMESVIRFLDPNFEGLERLKMGMYPRFTSGLFNKAIQVMYNTLVSNWNTEYARLLCNLPFEVIKDLLESDKLTVGASSMARYKLANEIVKMRASFRKQNHIEGKGDESVVLAFDEGSRGIQLVHIAPGAESRRKIWKATSVR DE50CC8EACBF0AA7 tcpg_schpo T-complex protein 1 subunit gamma cct3 CCT-gamma SPBC1A4.08c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MQSPVFVMNTNGNRQVGHKAQMSNIQAAKAVADVIRTCLGPRAMLKMLLDPVGSVLLTNDGHAILREIEVAHPAAKSMIELARTQDEEVGDGTTSVIILAGEILAAASPLLDRKIHPVVMIRSFKQALEDALSIIDEITLPVNVDDNAEMFRLIRTCIGTKLVARWSDLMCHLALRAVRTVASTSNGRMEIDIKRYARVEKVPGGEIESSCVLDGVMLNKDVTHPKMRRRIENPRIVLLDCPLEYRKGESQTNIEISKDTDWNRILEIEEEQVKRMCDYIIAVKPDLVITEKGVSDLAQHYLLKANITALRRTRKSDNNRIARACGANIVNRLEDLREKDVGTGCGLFYIDKLGDEYYTFLTGCKNPKACTILLRGPSKDIINEVERNLQDAMAVARNVFFHPKLSPGGGATEMAVSVRLAEKARSIEGVAQWPYRAVADAIEIIPRTLVQNCGANPIKALTELRAKHAEGQHSFGIDGETGRVVDMHEYGVWEPEAVKLQSIKTAIESACLLLRVDDIVSGVRKHSE 260BC1A6DA295E8E cul3-adt 4 8 unspecified role unspecified role bait bait c-ter protein a tag protein a tag protein A protein A c-term range C-terminal range c-term range c-term range C-terminal range c-term range 14 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 19 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey association association Suppl Fig. S1 btb1-cul3 2 8 unspecified role unspecified role prey prey c-ter myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role bait bait c-ter protein a tag protein a tag protein A protein A c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 1B btb2-cul3 2 18 unspecified role unspecified role bait bait c-ter protein a tag protein a tag protein A protein A c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role prey prey c-ter myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 1C btb3-cul3 2 8 unspecified role unspecified role prey prey c-ter myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role bait bait c-ter protein a tag protein a tag protein A protein A c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 1C btb1-pip1 3 8 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait c-ter protein a tag protein a tag protein A protein A c-term range C-terminal range c-term range c-term range C-terminal range c-term range 7 unspecified role unspecified role prey prey association association Fig. 1D btb2-pip1 1 8 unspecified role unspecified role prey prey 18 unspecified role unspecified role bait bait n-ter myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 7 unspecified role unspecified role prey prey association association Fig. 1E btb3-pip1 3 8 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait c-ter myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 7 unspecified role unspecified role prey prey association association Fig. 1D btb3-cul3-2 1 8 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q10225:p.His181Leu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain H L Wed May 02 15:31:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his181leu' Tue Jun 30 18:28:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His181Leu' Q10225:p.Asp168Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain D A Wed May 02 16:26:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp168ala' Tue Jun 30 17:29:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp168Ala' physical association physical association Fig. 2A and Fig. 2B btb3-cul3-3 2 8 unspecified role unspecified role prey prey n-ter myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q09760:p.Tyr50Gly mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain Y G Wed May 02 17:40:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr50gly' Tue Jun 30 18:47:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr50Gly' 10 unspecified role unspecified role bait bait c-ter protein a tag protein a tag protein A protein A c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 3C