IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The MEP-1 zinc-finger protein acts with MOG DEAH box proteins to control gene expression via the fem-3 3' untranslated region in Caenorhabditis elegans. The MEP-1 zinc-finger protein acts with MOG DEAH box proteins to control gene expression via the fem-3 3' untranslated region in Caenorhabditis elegans. RNA (1355-8382) 2002 imex curation Belfiore M., Mathies LD., Pugnale P., Moulder G., Barstead R., Kimble J., Puoti A. The email address entered is an address for "requests for reprints" - no other address given. Alessandro+Puoti@unifr.ch. lACT-RB2 cDNA library from mixed staged C. elegans hermaphrodites. jyoti Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Belfiore M., Mathies LD., Pugnale P., Moulder G., Barstead R., Kimble J., Puoti A. The email address entered is an address for "requests for reprints" - no other address given. The email address entered is an address for "requests for reprints" - no other address given. Alessandro+Puoti@unifr.ch. Accepted 2011-MAR-25 by JYOTI lACT-RB2 cDNA library from mixed staged C. elegans hermaphrodites. RNA (1355-8382) 2002 jyoti Only protein-protein interactions imex curation The MEP-1 zinc-finger protein acts with MOG DEAH box proteins to control gene expression via the fem-3 3' untranslated region in Caenorhabditis elegans. The MEP-1 zinc-finger protein acts with MOG DEAH box proteins to control gene expression via the fem-3 3' untranslated region in Caenorhabditis elegans. RNA (1355-8382) 2002 imex curation Belfiore M., Mathies LD., Pugnale P., Moulder G., Barstead R., Kimble J., Puoti A. The email address entered is an address for "requests for reprints" - no other address given. Alessandro+Puoti@unifr.ch. lACT-RB2 cDNA library from mixed staged C. elegans hermaphrodites. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Belfiore M., Mathies LD., Pugnale P., Moulder G., Barstead R., Kimble J., Puoti A. The email address entered is an address for "requests for reprints" - no other address given. Alessandro+Puoti@unifr.ch. Accepted 2011-MAR-25 by JYOTI lACT-RB2 cDNA library from mixed staged C. elegans hermaphrodites. RNA (1355-8382) 2002 jyoti Only protein-protein interactions imex curation The MEP-1 zinc-finger protein acts with MOG DEAH box proteins to control gene expression via the fem-3 3' untranslated region in Caenorhabditis elegans. The MEP-1 zinc-finger protein acts with MOG DEAH box proteins to control gene expression via the fem-3 3' untranslated region in Caenorhabditis elegans. RNA (1355-8382) 2002 imex curation Belfiore M., Mathies LD., Pugnale P., Moulder G., Barstead R., Kimble J., Puoti A. The email address entered is an address for "requests for reprints" - no other address given. Alessandro+Puoti@unifr.ch. lACT-RB2 cDNA library from mixed staged C. elegans hermaphrodites. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Belfiore M., Mathies LD., Pugnale P., Moulder G., Barstead R., Kimble J., Puoti A. The email address entered is an address for "requests for reprints" - no other address given. The email address entered is an address for "requests for reprints" - no other address given. Alessandro+Puoti@unifr.ch. Accepted 2011-MAR-25 by JYOTI lACT-RB2 cDNA library from mixed staged C. elegans hermaphrodites. RNA (1355-8382) 2002 jyoti Only protein-protein interactions imex curation mog1_caeel Probable pre-mRNA-splicing factor ATP-dependent RNA helicase mog-1 mog-1 Masculinization of germline protein 1 Sex determination protein mog-1 K03H1.2 protein protein caeel Caenorhabditis elegans MSDKRADGRLEGTSDTFGGLVIKKKKVEGDSKPTEPSGKSLLGLDRLASTKREHARKRLEDDDDRGVTESVRKGIEKVHEKHRDRDDRGMKYKSRDDDRRRDRDRSERREPSSRRGWKDRSGDQTPRFKVPDTPSRMSWDQDDREGSSRKRNSWDMPTPRGERDRKRYMDSERSISSAWRSERRNRDDEKRRRHRKPEDSVRSVKEEKAEPTFHDDEERAQWEEEQKNLDREWYDNEGAFDDEYNPFNKVSDEFVEKREKQWQEKTQKPRLTVKQQAIKRENELWENNRLHRSGVVAMADELSSIFEDETDENRVTILVQNIVPPFLDGRIVFTKQAQPIIPVVDTTCDMAVSAARGSVAVRKRREVEDRKKAQDKHWELAGSKLGNLMGVKEKKDETADPEDDDSGNYKESHQFASHMKDNEAVSDFAMEKSIKQQREYLPVFACRQKMMNVIRENNVVIIVGETGSGKTTQLAQYLLEDGFGDSGLIGCTQPRRVAAMSVARRVADEMGVDLGQDVGYAIRFEDCTSEKTIIKYMTDGILLRECLGDGSLDQYSAIIMDEAHERSLNTDVLFGLLREVIAKRADLKLIVTSATMDADKFADFFGGNCPTFTIPGRTFPVELFHARTPVEDYVDAAVKQAVTIHLGGMDGDILIFMPGQEDIECTCEMIKEKLGELDEAPPLAVLPIYSQLPSDLQAKIFQRAPGGMRKAIVATNIAETSLTVDGILFVIDPGFCKMKVYNPRIGMDALSIFPVSQASANQRTGRAGRTGPGQCYRLYTERQFKDELLKSTVPEIQRTNLANVVLLLKSLGVDDLLKFHFMDAPPQDNMLNSMYQLWTLGALDNTGQLTPMGRKMVEFPLDPTLSKMLIMSAEMGCSDEVLTIVSMLSVPAIFFRPKGREEEADAKKEKFQVPESDHLTFLNVYIQWRTHKYSAKWCADNYLHVKALKKVREVRAQLKEIMQDLKLPLISNGSEWDIVRKCICSAYFHNAARLKGIGEYVNVRTGIPCFLHPTSALFGMGFMPDYVVYHELIMTAKEYMQCVTAVDAIWLAELGPMFYSIKESKQSRKELKMESVRTVETMEAEMREAQKEMERRKEESDKAFKRPESSRRVVEVGSKSARSERRKLWGL 258C85D6584FAF3A dhx16_caeel Probable pre-mRNA-splicing factor ATP-dependent RNA helicase mog-4 mog-4 C04H5.6 Sex determination protein mog-4 Masculinization of germline protein 4 protein protein caeel Caenorhabditis elegans MSVEQFINDQLHSIVGISDRSICQYVHALAKKAKSAPDLVEKLRDAGDFPISPAIQSFADQLMSRMPRQATSARQRGPTTAELAEQELNRLNRAVGVLEDYSASSTKTKNVRKRKESSSEDDEAPIKASKPGKSVKPSKSDDSESDIEAMEAKLDADIAERDALAARINKKEKDKTRNVMEKKRDDNKDKEGSSMDKLREESRRQYLKKRKVDKLEELEAIVHDDQTLFAREKLTKREKADMEYRKKVLEYTKAHGKAGDVMKMKRYHLPDASTKQIPSQYVEDDEEDFRPGGDGAKWEEEQLMASMLHLGAKDAKRKEQEFELLLDEKVDFIQALQMPGTNEEVVETEAEKKKMSIEETRKSLPVYAFRDAFIEAVKEHQVLIIEGETGSGKTTQLPQYLYEAGFCEGGKRIGCTQPRRVAAMSVAARVADEVGCKLGTQVGYSIRFEDCTSEKTVLKYMTDGMLLREFLNEPDLASYSVMMIDEAHERTLHTDILFGLVKDIARFRKDLKLLISSATLDAEKFSSFFDDAPIFRIPGRRFPVDIYYTQAPEADYVDAAIVTIMQIHLTQPLPGDILVFLTGQEEIETVQEALMERSKALGSKIKELIPLPVYANLPSDLQAKIFEPTPKDARKVVLATNIAETSVTIDGINYVIDPGFSKQNSFDARSGVEHLHVVTISKAAANQRAGRAGRTGPGKCFRLYTAWAYKHELEEQPIPEIQRTNLGNVVLMLKSLGIHDLVHFDFLDPPPQETLVIALEQLYALGALNHRGELTKLGRRMAEFPCDPCMSKMIIASEKYECSEEIVTIAAMLSCNAAVFYRPKAQVIHADSARKGFWSPAGDHITLMNVYNKWQESSFSQRWCVENYVQHRTMKRARDVRDQLVGLLERVEIETKSSTDTIKIRKAITAGYFYNVSKLDNTGHYKTVKHKHTTHPHPNSCLFEETPRWVVYFELVFTSKEFMREMSEIESGWLLEVAPHYYKGRELEDATNKKMPKNKGKSGKDLER F6EB913D919FFD5D mog5_caeel Probable pre-mRNA-splicing factor ATP-dependent RNA helicase mog-5 mog-5 EEED8.5 Sex determination protein mog-5 Masculinization of germline protein 5 protein protein caeel Caenorhabditis elegans MDQLEHLSLVSKVLSEVENHFGVVEKDVAEFVIHLAQENPTFDKLKKALDSQGLGDQFDDSLTATILRIVQSMTAVKKKNKKGGDSKEDIKDSHKITLISDAKEEIKARLPALAMANTAKEKEDNEDDLMAQLEKMEGRYESEKRLQKESDSKRNRSRSRSRSRDRKRRRSRSGDRDRRRRSRSREDRRDRDRRDRDRDRDRGRGDRRGDDRQRDRRDRRDDGGARKSEVAEIGKIYDGRVNSIQSFGAFITLEGFRQKQEGLVHISQIRNERVQTVADVLKRGENVKVKVNKIENGKISLSMKEVDQNSGEDLNPRETDLNPDAIGVRPRTPPASTSSWMNPEASGVGQGPSTSIGGGKARVRISTPERWELRQMQGAGVLTATDMPDFDEEMGVLRNYDDESDGEDIEIELVEDEPDFLRGYGKGGAEIEPVKVVKNPDGSLAQAALMQGALSKERKETKIQAQRERDMDTQKGFSSNARILDPMSGNQSTAWSADESKDRNNKMKEMPEWLKHVTAGGKATYGRRTNLSMVEQRESLPIFALKKNLMEAMIDNQILVVVGETGSGKTTQMTQYAIEAGLGRRGKIGCTQPRRVAAMSVAKRVAEEYGCKLGTDVGYTIRFEDCTSQDTIIKYMTDGMLLRECLIDPDLSGYSLIMLDEAHERTIHTDVLFGLLKAAARKRPELKLIITSATLDSVKFSEYFLEAPIFTIPGRTFPVEILYTREPESDYLEAAHITVMQIHLTEPPGDVLVFLTGQEEIDTSCEVLYERMKSMGPDVPELIILPVYGALPSEMQTRIFDPAPAGKRKVVIATNIAETSLTIDGIFYVVDPGFVKQKIYNPKSGMDSLVVTPISQAAAKQRSGRAGRTGPGKCYRLYTERAFRDEMLPTPVPEIQRTNLASTLLQLKAMGINNLIDFDFMDAPPLDSMITALNTLHTLSALDGDGLLTKLGRRMAEFPLEPSLSKLLIMSVDLGCSEEVLTIVAMLNVQNIFYRPKEKQDHADQKKAKFHQPEGDHLTLLAVYNSWKNHHFSQPWCFENFIQVRSMKRAQDIRKQLLGIMDRHKLLMVSCGRDVSRVQKAICSGFFRNAAKRDPQEGYRTLTDGQNVYIHPSSACFQQQPEWVVYHELVMTTKEYMREVTAIDPKWLVEFAPSFFKIGDSTKLSTFKRNQKIDPLFDKYADANAWRITRVKKRIYNPNK 023C46E494D7F44F mep1_caeel MOG interacting and ectopic P-granules protein 1 M04B2.1 Nuclear zinc finger protein mep-1 protein protein caeel Caenorhabditis elegans MVTADETVLATTTNTTSMSVEPTDPRSAGESSSDSEPDTIEQLKAEQREVMADAANGSEVNGNQENGKEEAASADVEVIEIDDTEESTDPSPDGSDENGDAASTSVPIEEEARKKDEGASEVTVASSEIEQDDDGDVMEITEEPNGKSEDTANGTVTEEVLDEEEPEPSVNGTTEIATEKEPEDSSMPVEQNGKGVKRPVECIELDDDDDDEIQEISTPAPAKKAKIDDVKATSVPEEDNNEQAQKRLLDKLEEYVKEQKDQPSSKSRKVLDTLLGAINAQVQKEPLSVRKLILDKVLVLPNTISFPPSQVCDLLIEHDPEMPLTKVINRMFGEERPKLSDSEKRERAQLKQHNPVPNMTKLLVDIGQDLVQEATYCDIVHAKNLPEVPKNLETYKQVAAQLKPVWETLKRKNEPYKLKMHRCDVCGFQTESKLVMSTHKENLHFTGSKFQCTMCKETDTSEQRMKDHYFETHLVIAKSEEKESKYPCAICEEDFNFKGVREQHYKQCKKDYIRIRNIMMPKQDDHLYINRWLWERPQLDPSILQQQQQAALQQAQQKKQQQLLHQQQAAQAAAAAQLLRKQQLQQQQQQQQARLREQQQAAQFRQVAQLLQQQSAQAQRAQQNQGNVNHNTLIAAMQASLRRGGQQGNSLAVSQLLQKQMAALKSQQGAQQLQAAVNSMRSQNSQKTPTHRSSKLVTTPSHATVGSSSAPTFVCEICDASVQEKEKYLQHLQTTHKQMVGKVLQDMSQGAPLACSRCRDRFWTYEGLERHLVMSHGLVTADLLLKAQKKEDGGRCKTCGKNYAFNMLQHLVADHQVKLCSAEIMYSCDVCAFKCSSYQTLEAHLTSNHPKGDKKTSTPAKKDDCITLDD 3BF8924F86B5A6E3 mog1-mep_1 2 7 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association Fig 1B mog_5-mep_1 2 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association Fig 1B mep_1-mep_1 2 7 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Fig 1B mog1-mep_1-1 3 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain 4 unspecified role unspecified role bait bait physical association physical association Fig 2A mog_4-mep_1 3 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain 5 unspecified role unspecified role bait bait physical association physical association Fig 2A mog_5-mep_1-1 3 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain 6 unspecified role unspecified role bait bait physical association physical association Fig 2A mep_1-mep_1-1 3 7 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Fig 2A mog_4-mog1 3 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association Fig 2C this interaction was found to be RNA dependent mep_1-mog1 1 4 unspecified role unspecified role prey prey 35s-met 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 2B, 3B. mep_1-mog_4 1 5 unspecified role unspecified role prey prey 35s-met 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 2B, 3B. mep_1-mog_5 1 6 unspecified role unspecified role prey prey 35 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 2B, 3B. mep_1-mep_1-2 1 7 unspecified role unspecified role prey prey 35s-met 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 2B, 3B. mog_4-mog1-1 1 4 unspecified role unspecified role prey prey 35s-met 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 3C. RNAse mog_4-mog_4 1 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey 35s-met 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction Fig 3C. RNAse mog_4-mog_5 1 6 unspecified role unspecified role prey prey 35s-met 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 3C. RNAse