UniProt UniProt http://www.uniprot.org Protein-protein interactions of hCsl4p with other human exosome subunits. Protein-protein interactions of hCsl4p with other human exosome subunits. J. Mol. Biol. (0022-2836) 2002 imex curation Raijmakers R., Noordman YE., van Venrooij WJ., Pruijn GJ. G.Pruijn@ncmls.kun.nl orchard Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Raijmakers R., Noordman YE., van Venrooij WJ., Pruijn GJ. no fig 3 includes further interactions bases on MW and crossreactivity of the antibody. Not included. G.Pruijn@ncmls.kun.nl Accepted 2011-MAR-24 by ORCHARD J. Mol. Biol. (0022-2836) 2002 orchard Only protein-protein interactions imex curation Protein-protein interactions of hCsl4p with other human exosome subunits. Protein-protein interactions of hCsl4p with other human exosome subunits. J. Mol. Biol. (0022-2836) 2002 imex curation Raijmakers R., Noordman YE., van Venrooij WJ., Pruijn GJ. G.Pruijn@ncmls.kun.nl orchard Only protein-protein interactions chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Raijmakers R., Noordman YE., van Venrooij WJ., Pruijn GJ. G.Pruijn@ncmls.kun.nl Accepted 2011-MAR-24 by ORCHARD J. Mol. Biol. (0022-2836) 2002 orchard Only protein-protein interactions imex curation Protein-protein interactions of hCsl4p with other human exosome subunits. Protein-protein interactions of hCsl4p with other human exosome subunits. J. Mol. Biol. (0022-2836) 2002 imex curation Raijmakers R., Noordman YE., van Venrooij WJ., Pruijn GJ. G.Pruijn@ncmls.kun.nl orchard Only protein-protein interactions in vitro In vitro pull down pull down autoradiography autoradiography Raijmakers R., Noordman YE., van Venrooij WJ., Pruijn GJ. G.Pruijn@ncmls.kun.nl Accepted 2011-MAR-24 by ORCHARD J. Mol. Biol. (0022-2836) 2002 orchard Only protein-protein interactions imex curation exos1_human Exosome complex component CSL4 CSL4 EXOSC1 CGI-108 Exosome component 1 protein protein human Homo sapiens Human MAPPVRYCIPGERLCNLEEGSPGSGTYTRHGYIFSSLAGCLMKSSENGALPVVSVVRETESQLLPDVGAIVTCKVSSINSRFAKVHILYVGSMPLKNSFRGTIRKEDVRATEKDKVEIYKSFRPGDIVLAKVISLGDAQSNYLLTTAENELGVVVAHSESGIQMVPISWCEMQCPKTHTKEFRKVARVQPEFLQT DIP protein Q9Y3B2 original sequence version: 130 E9C3B0A66F911195 exosx_human Exosome component 10 PMSCL2 EXOSC10 PMSCL Polymyositis/scleroderma autoantigen 2 Autoantigen PM/Scl 2 Polymyositis/scleroderma autoantigen 100 kDa P100 polymyositis-scleroderma overlap syndrome-associated autoantigen RRP6 protein protein human Homo sapiens Human MAPPSTREPRVLSATSATKSDGEMVLPGFPDADSFVKFALGSVVAVTKASGGLPQFGDEYDFYRSFPGFQAFCETQGDRLLQCMSRVMQYHGCRSNIKDRSKVTELEDKFDLLVDANDVILERVGILLDEASGVNKNQQPVLPAGLQVPKTVVSSWNRKAAEYGKKAKSETFRLLHAKNIIRPQLKFREKIDNSNTPFLPKIFIKPNAQKPLPQALSKERRERPQDRPEDLDVPPALADFIHQQRTQQVEQDMFAHPYQYELNHFTPADAVLQKPQPQLYRPIEETPCHFISSLDELVELNEKLLNCQEFAVDLEHHSYRSFLGLTCLMQISTRTEDFIIDTLELRSDMYILNESLTDPAIVKVFHGADSDIEWLQKDFGLYVVNMFDTHQAARLLNLGRHSLDHLLKLYCNVDSNKQYQLADWRIRPLPEEMLSYARDDTHYLLYIYDKMRLEMWERGNGQPVQLQVVWQRSRDICLKKFIKPIFTDESYLELYRKQKKHLNTQQLTAFQLLFAWRDKTARREDESYGYVLPNHMMLKIAEELPKEPQGIIACCNPVPPLVRQQINEMHLLIQQAREMPLLKSEVAAGVKKSGPLPSAERLENVLFGPHDCSHAPPDGYPIIPTSGSVPVQKQASLFPDEKEDNLLGTTCLIATAVITLFNEPSAEDSKKGPLTVAQKKAQNIMESFENPFRMFLPSLGHRAPVSQAAKFDPSTKIYEISNRWKLAQVQVQKDSKEAVKKKAAEQTAAREQAKEACKAAAEQAISVRQQVVLENAAKKRERATSDPRTTEQKQEKKRLKISKKPKDPEPPEKEFTPYDYSQSDFKAFAGNSKSKVSSQFDPNKQTPSGKKCIAAKKIKQSVGNKSMSFPTGKSDRGFRYNWPQR DIP protein Q01780 original sequence version: 154 A37BDC8F49BF2E57 exos7_human Exosome complex component RRP42 KIAA0116 RRP42 EXOSC7 Ribosomal RNA-processing protein 42 Exosome component 7 p8 protein protein human Homo sapiens Human MASVTLSEAEKVYIVHGVQEDLRVDGRGCEDYRCVEVETDVVSNTSGSARVKLGHTDILVGVKAEMGTPKLEKPNEGYLEFFVDCSASATPEFEGRGGDDLGTEIANTLYRIFNNKSSVDLKTLCISPREHCWVLYVDVLLLECGGNLFDAISIAVKAALFNTRIPRVRVLEDEEGSKDIELSDDPYDCIRLSVENVPCIVTLCKIGYRHVVDATLQEEACSLASLLVSVTSKGVVTCMRKVGKGSLDPESIFEMMETGKRVGKVLHASLQSVVHKEESLGPKRQKVGFLG DIP protein Q15024 original sequence version: 123 DIP protein Q15024 original sequence version: 138 A674F745CEC61BBB exos5_human Exosome complex component RRP46 EXOSC5 CML28 RRP46 Ribosomal RNA-processing protein 46 Exosome component 5 p12B Chronic myelogenous leukemia tumor antigen 28 protein protein human Homo sapiens Human MEEETHTDAKIRAENGTGSSPRGPGCSLRHFACEQNLLSRPDGSASFLQGDTSVLAGVYGPAEVKVSKEIFNKATLEVILRPKIGLPGVAEKSRERLIRNTCEAVVLGTLHPRTSITVVLQVVSDAGSLLACCLNAACMALVDAGVPMRALFCGVACALDSDGTLVLDPTSKQEKEARAVLTFALDSVERKLLMSSTKGLYSDTELQQCLAAAQAASQHVFRFYRESLQRRYSKS DIP protein Q9NQT4 original sequence version: 123 DIP protein Q9NQT4 original sequence version: 66 DIP protein Q9NQT4 original sequence version: 112 DIP protein Q9NQT4 original sequence version: 129 DIP protein Q9NQT4 original sequence version: 105 C3C1FCB39D85B1EE exosc1-exosc7-2 2 6 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association 1 exosc1-exosc5-5 2 7 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association 1 exosc1-exosc7-3 3 6 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 4 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 2b exosc1-exosc5-6 3 7 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 4 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 2b exosc1-exosc10-1 1 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3 exosc7-exosc10 1 6 unspecified role unspecified role bait bait region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey physical association physical association 3 exosc5-exosc10 1 7 unspecified role unspecified role bait bait region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey physical association physical association 3 exosc7-exosc1 2 6 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association 1 exosc5-exosc1-2 2 4 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association 1