UniProt UniProt http://www.uniprot.org The Bbp1p-Mps2p complex connects the SPB to the nuclear envelope and is essential for SPB duplication. The Bbp1p-Mps2p complex connects the SPB to the nuclear envelope and is essential for SPB duplication. EMBO J. (0261-4189) 2000 imex curation Schramm C., Elliott S., Shevchenko A., Schiebel E. eschiebe@udcf.gla.ac.uk 06-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down fingerprinting peptide massfingerprinting fingerprinting Schramm C., Elliott S., Shevchenko A., Schiebel E. eschiebe@udcf.gla.ac.uk Accepted 2011-APR-20 by ORCHARD 06-Jun-2011: Contacted by JYOTI. EMBO J. (0261-4189) 2000 orchard Only protein-protein interactions imex curation The Bbp1p-Mps2p complex connects the SPB to the nuclear envelope and is essential for SPB duplication. The Bbp1p-Mps2p complex connects the SPB to the nuclear envelope and is essential for SPB duplication. EMBO J. (0261-4189) 2000 imex curation Schramm C., Elliott S., Shevchenko A., Schiebel E. eschiebe@udcf.gla.ac.uk 06-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Schramm C., Elliott S., Shevchenko A., Schiebel E. eschiebe@udcf.gla.ac.uk Accepted 2011-APR-20 by ORCHARD 06-Jun-2011: Contacted by JYOTI. EMBO J. (0261-4189) 2000 orchard Only protein-protein interactions imex curation The Bbp1p-Mps2p complex connects the SPB to the nuclear envelope and is essential for SPB duplication. The Bbp1p-Mps2p complex connects the SPB to the nuclear envelope and is essential for SPB duplication. EMBO J. (0261-4189) 2000 imex curation Schramm C., Elliott S., Shevchenko A., Schiebel E. eschiebe@udcf.gla.ac.uk 06-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Schramm C., Elliott S., Shevchenko A., Schiebel E. eschiebe@udcf.gla.ac.uk Accepted 2011-APR-20 by ORCHARD 06-Jun-2011: Contacted by JYOTI. EMBO J. (0261-4189) 2000 orchard Only protein-protein interactions imex curation The Bbp1p-Mps2p complex connects the SPB to the nuclear envelope and is essential for SPB duplication. The Bbp1p-Mps2p complex connects the SPB to the nuclear envelope and is essential for SPB duplication. EMBO J. (0261-4189) 2000 imex curation Schramm C., Elliott S., Shevchenko A., Schiebel E. eschiebe@udcf.gla.ac.uk 06-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Schramm C., Elliott S., Shevchenko A., Schiebel E. eschiebe@udcf.gla.ac.uk Accepted 2011-APR-20 by ORCHARD 06-Jun-2011: Contacted by JYOTI. EMBO J. (0261-4189) 2000 orchard Only protein-protein interactions imex curation The Bbp1p-Mps2p complex connects the SPB to the nuclear envelope and is essential for SPB duplication. The Bbp1p-Mps2p complex connects the SPB to the nuclear envelope and is essential for SPB duplication. EMBO J. (0261-4189) 2000 imex curation Schramm C., Elliott S., Shevchenko A., Schiebel E. eschiebe@udcf.gla.ac.uk 06-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Schramm C., Elliott S., Shevchenko A., Schiebel E. eschiebe@udcf.gla.ac.uk Accepted 2011-APR-20 by ORCHARD 06-Jun-2011: Contacted by JYOTI. EMBO J. (0261-4189) 2000 orchard Only protein-protein interactions imex curation mps2_yeast Monopolar spindle protein 2 YGL075C MPS2 MMC1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNGAFDAIFEYAWGQIDKPISGDFIYGKDLPKLIEIIENIFQKAQKSGSYELRLPLFSEINKDLFRTFSNTKTFFKIHKEEFDDIFFNLVNHPLREILENAFIGVDSIPSDFIVSMNLNSPSKFLVENKNKNTEGAGISTPRKKLTESPIKLLSRNNIGKALEVQVEELKRELTAKQSLLQENERQVSELKIRLETYQEKYASIQQRFSDLQKARQVEDNQNSSRTSDPGSPLVTGIDQKAILEEFRRRLQRQTDTISFLKDQIRRERGLNCSNDKVSHSKRKHATTDGDGTFKNFISAVPSNIWVKATIRIIVCFALLAGVLPYIRKYVYAHDTPSQNSRLQLSWWENSGILSKIVWFFEDQTDLETEYRSNANVDDAYSRVFGI CF8AFA86EDEA834C spc29_yeast Spindle pole component 29 SPC29 LPH3 YPL124W LPH3W NIP29 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDYSNFGNSASKKFQDDTLNRVRKEHEEALKKLREENFSSNTSELGNKKHYRAQERMSSPLHRLSPTGKSDDRKVKSPLDDKLRRQLREGNTRLPPPPFSSYGMPPTNRSNLDRIRRRTSSPVRTDKFASQNVIDDQRLEIKYLERIVYDQGTVIDNLTSRITRLESFILNSISDRGDKNFASLEHSRSFSGFPTNKTYGLQMGGLYENDMPYRRSSDNINKEGAREDRSSQIHIENESTEDILKILSSSFHN 0730B17A86136621 0730B17A86136621 kar1_yeast Cell division control protein KAR1 N1611 YNL188W KAR1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNVTSPKDGNHSFSKKNRFNTNKPRFHKLNEQAQSINLPEDRDSIVSSNTTSIMTDDAFDYNEGIASRTKNINSDSDRSNDTIKQNNYNKRETGYNPFYNGSGINQRYTQFRKREFEPTLAENKAEEYISDEDNVKIDEDNIENELQFTPKIKEASILRSSLLGQRNVLNTRNPKSKESHIKVKPIINNKSSSQRKSSAALRKQLGKPLPLPYLNSPNSDSTPTLQRKEEVFTDEVLQKKRELIESKWHRLLFHDKKMVEKKLESLREYERKRMPPRGTDVSSSEQDNSFKISTPTKSYVSLEQKPLPNLSAMNNFNDVTDNKEKEETNNNILKFQAQRDPLQILQSEIEMHTKKLDTIIELLKDDTDSKEKRKVVTNDNAAPEQMVNKGWRKNVMMIYKKSGNIMKKYREYFLWTICILILLYCNIYVYYRF D65331B3B2B068E4 bbp1_yeast Spindle pole component BBP1 BBP1 YPL255W P0745 BFR1-binding protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNQEDNTGGGGIFGLFKWTKDALFGTDISPSMKYKDQEERRDRSRYAQDDTNFSMKFGNDSNRRSTNLSRSNSWSGLDSTLHRKYELLPEYNENGFNSIVNGDHHSKERIRSLRSPAPIVPREPLRNEPTDTFGHRLHTKRRTINELSNSQIPFIPPQEDDPLLSKLFNKDGVNEVRRSPYKLSVKDIPGKFPSPLTKRDEIDNYYVRDEDACHKNREYKKAYFDLFAQMDLNSRDLEDLCEDVREQREQFHRNEQTYKQAYEEMRAELVNELKKSKTLFENYYSLGQKYKSLKKVLDQTISHEAELATSRERLYQEEDLKNFEIQTLKQRLSDLELKYTNLQIEKDMQRDNYESEIHDLLLQLSLRNNERKDTSAGSNIFSTGQ 3A1A827E7664E4E0 3A1A827E7664E4E0 bbp1-bbp1 5 9 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association Fig. 1B and 6E bbp1-nip29 5 9 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Fig. 1B nip29-bbp1 5 7 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association Fig. 1B nip29-nip29-6 5 7 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Fig. 1B ygh5-bbp1 5 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association Fig. 6E bbp1-ygh5 5 9 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association Fig. 6E ygh5-ygh5 5 6 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association Fig. 6E bbp1-kar1 5 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association Fig. 8B bbp1-nip29-1 4 9 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction Fig. 1C bbp1-ygh5-1 1 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 6A mps2-bbp1-1 2 9 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 6C bbp1-ygh5-2 3 6 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 7