IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Insights into egg coat assembly and egg-sperm interaction from the X-ray structure of full-length ZP3. Insights into egg coat assembly and egg-sperm interaction from the X-ray structure of full-length ZP3. Cell (0092-8674) 2010 imex curation Han L., Monne' M., Okumura H., Schwend T., Cherry AL., Flot D., Matsuda T., Jovine L. Proteins purified by nickel chromatography and identified by anti-tag western blot prior to size exclusion chromatography on Superdex 200 10/300 GL column. luca.jovine@ki.se 06-April-2011: Contacted by JYOTI. jyoti Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink weight by staining molecular weight estimation by staining weight by staining Han L., Monne' M., Okumura H., Schwend T., Cherry AL., Flot D., Matsuda T., Jovine L. Proteins purified by nickel chromatography and identified by anti-tag western blot prior to size exclusion chromatography on Superdex 200 10/300 GL column. luca.jovine@ki.se Accepted 2010-Not a month!-8 by JYOTI. 06-April-2011: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions imex curation Insights into egg coat assembly and egg-sperm interaction from the X-ray structure of full-length ZP3. Insights into egg coat assembly and egg-sperm interaction from the X-ray structure of full-length ZP3. Cell (0092-8674) 2010 imex curation Han L., Monne' M., Okumura H., Schwend T., Cherry AL., Flot D., Matsuda T., Jovine L. Proteins purified by nickel chromatography and identified by anti-tag western blot prior to size exclusion chromatography on Superdex 200 10/300 GL column. luca.jovine@ki.se 06-April-2011: Contacted by JYOTI. jyoti Only protein-protein interactions crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Han L., Monne' M., Okumura H., Schwend T., Cherry AL., Flot D., Matsuda T., Jovine L. Proteins purified by nickel chromatography and identified by anti-tag western blot prior to size exclusion chromatography on Superdex 200 10/300 GL column. luca.jovine@ki.se Accepted 2010-Not a month!-8 by JYOTI. 06-April-2011: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions imex curation Insights into egg coat assembly and egg-sperm interaction from the X-ray structure of full-length ZP3. Insights into egg coat assembly and egg-sperm interaction from the X-ray structure of full-length ZP3. Cell (0092-8674) 2010 imex curation Han L., Monne' M., Okumura H., Schwend T., Cherry AL., Flot D., Matsuda T., Jovine L. Proteins purified by nickel chromatography and identified by anti-tag western blot prior to size exclusion chromatography on Superdex 200 10/300 GL column. luca.jovine@ki.se 06-April-2011: Contacted by JYOTI. jyoti Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Han L., Monne' M., Okumura H., Schwend T., Cherry AL., Flot D., Matsuda T., Jovine L. Proteins purified by nickel chromatography and identified by anti-tag western blot prior to size exclusion chromatography on Superdex 200 10/300 GL column. luca.jovine@ki.se Accepted 2010-Not a month!-8 by JYOTI. 06-April-2011: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions imex curation zp3_chick Zona pellucida sperm-binding protein 3 ZP3 ZPC Sperm receptor Zona pellucida C protein Zona pellucida glycoprotein 3 protein protein chick Gallus gallus (Chicken) Chicken MQGGRVVLGLLCCLVAGVGSYTPWDISWAARGDPSAWSWGAEAHSRAVAGSHPVAVQCQEAQLVVTVHRDLFGTGRLINAADLTLGPAACKHSSLNAAHNTVTFAAGLHECGSVVQVTPDTLIYRTLINYDPSPASNPVIIRTNPAVIPIECHYPRRENVSSNAIRPTWSPFNSALSAEERLVFSLRLMSDDWSTERPFTGFQLGDILNIQAEVSTENHVPLRLFVDSCVAALSPDGDSSPHYAIIDFNGCLVDGRVDDTSSAFITPRPREDVLRFRIDVFRFAGDNRNLIYITCHLKVTPADQGPDPQNKACSFNKARNTWVPVEGSRDVCNCCETGNCEPPALSRRLNPMERWQSRRFRRDAGKEVAADVVIGPVLLSADPGAVGQQEEGGDGAAVMVPSVGTGLVCVAVAVALAAVGVAVGIARKGCTRTSAAV A9B59E9DE71819B3 zp3 2 4 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain undefined mutation asn159gln_arg359_361_362ala mutation mutation certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain Wed Jul 06 16:32:41 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'mutated region' direct interaction direct interaction Radius of gyration (Rg) of 32.02 A. Supp. Fig. 1C zp3-1 1 4 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain undefined mutation asn159gln_arg359_361_362ala mutation mutation certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain Wed Jul 06 16:32:22 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'mutated region' c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. direct interaction direct interaction Supp. Fig. 1D Coomassie blue and silver stained SDS-PAGE gels show an 84 kDa band after crosslinking of cZP3-4 in solution with BS(PEG)9, equivalent to a dimeric species. zp3-2 3 4 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. direct interaction direct interaction Supp. Fig. 5, Supp. Table 1 Trypsinized cZP3-3 and cZP3-4 (cZP3-3T/4T) produced tetragonal crystals that diffracted to high resolution despite 71% solvent content (Figures S5A and S5B) 2.0-2.6A R working 21.9%, R free 24.9% (3NK3) Two molecules of ZP3 embrace each other in antiparallel orientation to form a flat, Yin-Yang-shaped homodimer