MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Regulation of clathrin adaptor function in endocytosis: novel role for the SAM domain. Regulation of clathrin adaptor function in endocytosis: novel role for the SAM domain. EMBO J. (0261-4189) 2010 imex curation Di Pietro SM., Cascio D., Feliciano D., Bowie JU., Payne GS. santiago.dipietro@colostate.edu Only protein-protein interactions in vitro In vitro competition binding competition binding predetermined predetermined participant predetermined Di Pietro SM., Cascio D., Feliciano D., Bowie JU., Payne GS. santiago.dipietro@colostate.edu EMBO J. (0261-4189) 2010 Only protein-protein interactions imex curation Regulation of clathrin adaptor function in endocytosis: novel role for the SAM domain. Regulation of clathrin adaptor function in endocytosis: novel role for the SAM domain. EMBO J. (0261-4189) 2010 imex curation Di Pietro SM., Cascio D., Feliciano D., Bowie JU., Payne GS. santiago.dipietro@colostate.edu Only protein-protein interactions in vitro In vitro fluorescence fluorescence technology fluorescence predetermined predetermined participant predetermined Di Pietro SM., Cascio D., Feliciano D., Bowie JU., Payne GS. santiago.dipietro@colostate.edu EMBO J. (0261-4189) 2010 Only protein-protein interactions imex curation Regulation of clathrin adaptor function in endocytosis: novel role for the SAM domain. Regulation of clathrin adaptor function in endocytosis: novel role for the SAM domain. EMBO J. (0261-4189) 2010 imex curation Di Pietro SM., Cascio D., Feliciano D., Bowie JU., Payne GS. santiago.dipietro@colostate.edu Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Di Pietro SM., Cascio D., Feliciano D., Bowie JU., Payne GS. santiago.dipietro@colostate.edu EMBO J. (0261-4189) 2010 Only protein-protein interactions imex curation Regulation of clathrin adaptor function in endocytosis: novel role for the SAM domain. Regulation of clathrin adaptor function in endocytosis: novel role for the SAM domain. EMBO J. (0261-4189) 2010 imex curation Di Pietro SM., Cascio D., Feliciano D., Bowie JU., Payne GS. santiago.dipietro@colostate.edu Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Di Pietro SM., Cascio D., Feliciano D., Bowie JU., Payne GS. santiago.dipietro@colostate.edu EMBO J. (0261-4189) 2010 Only protein-protein interactions imex curation Regulation of clathrin adaptor function in endocytosis: novel role for the SAM domain. Regulation of clathrin adaptor function in endocytosis: novel role for the SAM domain. EMBO J. (0261-4189) 2010 imex curation Di Pietro SM., Cascio D., Feliciano D., Bowie JU., Payne GS. santiago.dipietro@colostate.edu Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Di Pietro SM., Cascio D., Feliciano D., Bowie JU., Payne GS. santiago.dipietro@colostate.edu EMBO J. (0261-4189) 2010 Only protein-protein interactions imex curation sla1_yeast Actin cytoskeleton-regulatory complex protein SLA1 YBL0321 YBL007C SLA1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTVFLGIYRAVYAYEPQTPEELAIQEDDLLYLLQKSDIDDWWTVKKRVIGSDSEEPVGLVPSTYIEEAPVLKKVRAIYDYEQVQNADEELTFHENDVFDVFDDKDADWLLVKSTVSNEFGFIPGNYVEPENGSTSKQEQAPAAAEAPAATPAAAPASAAVLPTNFLPPPQHNDRARMMQSKEDQAPDEDEEGPPPAMPARPTATTETTDATAAAVRSRTRLSYSDNDNDDEEDDYYYNSNSNNVGNHEYNTEYHSWNVTEIEGRKKKKAKLSIGNNKINFIPQKGTPHEWSIDKLVSYDNEKKHMFLEFVDPYRSLELHTGNTTTCEEIMNIIGEYKGASRDPGLREVEMASKSKKRGIVQYDFMAESQDELTIKSGDKVYILDDKKSKDWWMCQLVDSGKSGLVPAQFIEPVRDKKHTESTASGIIKSIKKNFTKSPSRSRSRSRSKSNANASWKDDELQNDVVGSAAGKRSRKSSLSSHKKNSSATKDFPNPKKSRLWVDRSGTFKVDAEFIGCAKGKIHLHKANGVKIAVAADKLSNEDLAYVEKITGFSLEKFKANDGSSSRGTDSRDSERERRRRLKEQEEKERDRRLKERELYELKKARELLDEERSRLQEKELPPIKPPRPTSTTSVPNTTSVPPAESSNNNNSSNKYDWFEFFLNCGVDVSNCQRYTINFDREQLTEDMMPDINNSMLRTLGLREGDIVRVMKHLDKKFGRENIASIPTNATGNMFSQPDGSLNVATSPETSLPQQLLPQTTSPAQTAPSTSAETDDAWTVKPASKSESNLLSKKSEFTGSMQDLLDLQPLEPKKAAASTPEPNLKDLEPVKTGGTTVPAAPVSSAPVSSAPAPLDPFKTGGNNILPLSTGFVMMPMITGGDMLPMQRTGGFVVPQTTFGMQSQVTGGILPVQKTGNGLIPISNTGGAMMPQTTFGAAATVLPLQKTGGGLIPIATTGGAQFPQTSFNVQGQQQLPTGSILPVQKTANGLISANTGVSMPTVQRTGGTMIPQTSFGVSQQLTGGAMMTQPQNTGSAMMPQTSFNAVPQITGGAMMPQTSFNALPQVTGGAMMPLQRTGGALNTFNTGGAMIPQTSFSSQAQNTGGFRPQSQFGLTLQKTGGIAPLNQNQFTGGAMNTLSTGGVLQQQQPQTMNTFNTGGVMQELQMMTTFNTGGAMQQPQMMNTFNTDGIMQQPQMMNTFNTGGAMQQPQQQALQNQPTGFGFGNGPQQSRQANIFNATASNPFGF 7FD85AA776407624 7FD85AA776407624 gga2_yeast ADP-ribosylation factor-binding protein GGA2 YHR108W GGA2 Golgi-localized, gamma ear-containing, ARF-binding protein 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSHPHSHSIYLSELPVRKPQALGNPLLRKIQRACRMSLAEPDLALNLDIADYINEKQGAAPRDAAIALAKLINNRESHVAIFALSLLDVLVKNCGYPFHLQISRKEFLNELVKRFPGHPPLRYSKIQRLILTAIEEWYQTICKHSSYKNDMGYIRDMHRLLKYKGYAFPKISESDLAVLKPSNQLKTASEIQKEQEIAQAAKLEELIRRGKPEDLREANKLMKIMAGFKEDNAVQAKQAISSELNKLKRKADLLNEMLESPDSQNWDNETTQELHSALKVAQPKFQKIIEEEQEDDALVQDLLKFNDTVNQLLEKFNLLKNGDSNAASQIHPSHVSAPLQQSSGALTNEINLIDFNDLDEAPSQGNNNTNGTGTPAAAETSVNDLLGDLTDLSISNPSTANQASFGLGGDIVLGSSQPAPPVTTTNNSNNTLDLLGLSTPQSPTNSQAVNSSGFDLLMGFNPTTGTTTAPARTLVNQSPNLKIEFEISRESNSVIRIKSFFTNLSSSPISNLVFLLAVPKSMSLKLQPQSSNFMIGNAKDGISQEGTIENAPANPSKALKVKWKVNYSVNSTQAEETAVFTLPNV 7AC3A317AEE302E2 7AC3A317AEE302E2 clh_yeast Clathrin heavy chain CHC1 YGL206C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDLPIEFTELVDLMSLGISPQFLDFRSTTFESDHFVTVRETKDGTNSVAIVDLAKGNEVTRKNMGGDSAIMHPSQMVISVRANGTIVQIFNLETKSKLKSFTLDEPVIFWRWLSETTLGFVTARSILTSNVFDGNVNAKPQLLTLRHANLNNTQIINFVANKNLDWFAVVGILQENGRIAGRIQLFSKQRNISQAIDGHVAIFTNILLEGNGSTPVQVFVTGNRNATTGAGELRIIEIDHDASLPSQYQKETTDIFFPPDATNDFPIAVQVSEKYGIIYLLTKYGFIHLYELETGTNLFVNRITAESVFTAAPYNHENGIACINKKGQVLAVEISTSQIVPYILNKLSNVALALIVATRGGLPGADDLFQKQFESLLLQNDYQNAAKVAASSTSLRNQNTINRLKNIQAPPGAISPILLYFSTLLDKGKLNKEETIELARPVLQQDRKQLFEKWLKEDKLECSEELGDIVKPFDTTLALACYLRAGAHAKVISCLAELQQFEKIIPYCQKVGYQPNFLVLISSLIRSSPDRASEFAVSLLQNPETASQIDIEKIADLFFSQNHIQQGTSLLLDALKGDTPDQGHLQTRVLEVNLLHAPQVADAILGNNIFSHYDKPTIASLSEKAGLYQRALENYTDIKDIKRCVVHTNALPIDWLVGYFGKLNVEQSLACLKALMDNNIQANIQTVVQVATKFSDLIGPSTLIKLFEDYNATEGLYYYLASLVNLTEDKDVVYKYIEAAAKMKQYREIERIVKDNNVYDPERVKNFLKDANLEDQLPLVIVCDRFDFVHEMILYLYKSQNLKFIETYVQQVNPSKTAQVVGALLDMDCDEAFIQSLLQSVLGQVPINELTTEVEKRNRLKILLPFLEQSLSQGIQDQAVYNALAKIYIDSNNSPEKFLKENDQYDTLDVGHYCEKRDPYLAYIAYEKGQNDDDLIRITNENSMYKYQARYLLERSDLDLWNKVLNQENIHRRQLIDSVISVGIPELTDPEPVSLTVQAFMTNGLKLELIELLEKIILEPSPFNENVALQGLLLLSAIKYEPTKVSSYIEKLDNYDADEIAPLCIEHDLKEEAFEIYDKHEMYGKALKVLIEDIMSLDRAASYADKINTPELWSQIGTAQLDGLRIPDAIESYIKAEDPSNYENVIDIAEQAGKYEELIPFLLMARKTLKEPKIDGALILAYAELNKIHEIENLLAGSNVANLDHVGDKLFENKEYKAARLCYSAVSNYSKLASTLVYLGDYQAAVDTARKASNIKVWKLVNDACIEKKEFKLAQICGLNLIVHAEELDELVERYESNGYFEELISLFEAGLGLERAHMGMFTELAILYSKYEPDKTFEHLKLFWSRINIPKVIRAVEQAHLWSELVFLYAHYDEWDNAALTLIEKSTKDLDHAYFKEVVVKVSNLEIYYKAINFYVKFHPSLLVDLLTSLTPRLDIPRTVKIFSKSDNLPLIKPFLINVLPKNNSVVNQAYHDLMIEEEDYKALQDAVDSYDKFDQLGLASRLESHKLIFFKKIGALLYRRNKKWAKSLSILKEEKLWKDAIETAAISQDPKVVEALLTYFVETGNREGFVALLYAAYNLVRIEFVLEISWMNSLEDYIKPFEISIKKEQNDSIKKITEELAKKSGSNEEHKDGQPLMLMNSAMNVQPTGF 622F3083DF2FE315 622F3083DF2FE315 sla1-chc1 3 SLA1 6 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell P32790:p.Leu804Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Tue Jul 12 10:06:37 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 19:29:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu804ala' Tue Jun 30 15:52:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu804Ala' P32790:p.Leu803Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Tue Jul 12 10:06:30 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 19:04:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu803ala' Tue Jun 30 18:15:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu803Ala' P32790:p.Asp805Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D A Tue Jul 12 10:06:32 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 15:06:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp805ala' Tue Jun 30 20:05:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp805Ala' Chc 8 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association f1a mint sla1-chc1-1 4 Chc 8 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level SLA1 6 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified P32790:p.Asp805Ala mutation decreasing interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D A Tue Jul 12 10:06:03 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 14:40:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp805ala' Tue Jun 30 18:43:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp805Ala' P32790:p.Leu804Ala mutation decreasing interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Tue Jul 12 10:14:09 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 19:43:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu804ala' Tue Jun 30 16:57:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu804Ala' gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P32790:p.Leu803Ala mutation decreasing interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Tue Jul 12 10:53:28 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 18:00:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu803ala' Tue Jun 30 17:32:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu803Ala' physical association physical association mint f1c f1d f1f sla1-chc1-2 4 Chc 8 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level SLA1 6 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P32790:p.Leu804Ala mutation decreasing interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Tue Jul 12 10:13:50 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 16:41:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu804ala' Tue Jun 30 18:29:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu804Ala' gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P32790:p.Leu803Ala mutation decreasing interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Tue Jul 12 10:13:55 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 18:23:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu803ala' Tue Jun 30 18:54:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu803Ala' physical association physical association f1e mint sla1-sla1 4 SLA1 6 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Sla1 homology domain 2 SLA1 6 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified P32790:p.Asp805Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D A Tue Jul 12 10:14:22 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 14:31:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp805ala' Tue Jun 30 19:40:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp805Ala' P32790:p.Leu804Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Tue Jul 12 10:14:28 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 17:53:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu804ala' Tue Jun 30 20:10:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu804Ala' P32790:p.Leu803Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Tue Jul 12 10:14:15 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 17:39:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu803ala' Tue Jun 30 16:49:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu803Ala' binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction f1g mint gga2-sla1 4 Gga2 7 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal SLA1 6 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Sla1 homology domain 2 direct interaction direct interaction f1g mint sla1-sla1-1 4 SLA1 6 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified P32790:p.Arg708Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Tue Jul 12 10:23:08 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 14:42:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg708ala' Tue Jun 30 17:54:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg708Ala' gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Sla1 homology domain 2 SLA1 6 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Sla1 homology domain 2 P32790:p.Arg708Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Tue Jul 12 10:23:04 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 16:12:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg708ala' Tue Jun 30 18:32:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg708Ala' direct interaction direct interaction f4c mint sla1-sla1-2 4 SLA1 6 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified P32790:p.Glu681Ala mutation disrupting interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A Tue Jul 12 10:20:12 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 15:13:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu681ala' Tue Jun 30 15:37:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu681Ala' binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Sla1 homology domain 2 gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined SLA1 6 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Sla1 homology domain 2 P32790:p.Glu681Ala mutation disrupting interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A Tue Jul 12 10:22:56 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 18:27:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu681ala' Tue Jun 30 19:47:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu681Ala' his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction f4c mint sla1-sla1-3 1 SLA1 6 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Sla1 homology domain 2 SLA1 6 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association f1f mint sla1-sla1-4 1 SLA1 6 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified P32790:p.Leu803Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Tue Jul 12 10:20:51 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 17:15:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu803ala' Tue Jun 30 18:21:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu803Ala' P32790:p.Leu804Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Tue Jul 12 10:57:16 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 17:43:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu804ala' Tue Jun 30 19:27:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu804Ala' P32790:p.Asp805Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D A Tue Jul 12 10:20:49 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 15:42:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp805ala' Tue Jun 30 17:02:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp805Ala' binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain SLA1 6 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Sla1 homology domain 2 physical association physical association sf2 mint sla1-sla1-5 2 SLA1 6 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified P32790:p.Glu681Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A Tue Jul 12 10:50:00 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 17:32:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu681ala' Tue Jun 30 19:21:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu681Ala' his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Sla1 homology domain 2 SLA1 6 peptide synthesis peptide synthesis predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction mint f1h sla1-sla1-6 2 SLA1 6 peptide synthesis peptide synthesis predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain SLA1 6 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Sla1 homology domain 2 P32790:p.Met687Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain M A Tue Jul 12 10:07:03 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 15:31:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met687ala' Tue Jun 30 19:23:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met687Ala' his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction mint f1h sla1-sla1-7 5 SLA1 6 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell SLA1 6 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f4d mint