IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Involvement of Arabidopsis ROF2 (FKBP65) in thermotolerance. Involvement of Arabidopsis ROF2 (FKBP65) in thermotolerance. Plant Mol. Biol. (0167-4412) 2010 imex curation Meiri D., Tazat K., Cohen-Peer R., Farchi-Pisanty O., Aviezer-Hagai K., Avni A., Breiman A. adina@post.tau.ac.il 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Meiri D., Tazat K., Cohen-Peer R., Farchi-Pisanty O., Aviezer-Hagai K., Avni A., Breiman A. adina@post.tau.ac.il Accepted 2010-MAY-8 by ORCHARD. Plant Mol. Biol. (0167-4412) 2010 orchard Only protein-protein interactions imex curation Involvement of Arabidopsis ROF2 (FKBP65) in thermotolerance. Involvement of Arabidopsis ROF2 (FKBP65) in thermotolerance. Plant Mol. Biol. (0167-4412) 2010 imex curation Meiri D., Tazat K., Cohen-Peer R., Farchi-Pisanty O., Aviezer-Hagai K., Avni A., Breiman A. adina@post.tau.ac.il 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink weight silver stain molecular weight estimation by silver staining weight silver stain Meiri D., Tazat K., Cohen-Peer R., Farchi-Pisanty O., Aviezer-Hagai K., Avni A., Breiman A. Cross-linking agent Dithiobissuccinimidyl Propionate adina@post.tau.ac.il Accepted 2010-MAY-8 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Plant Mol. Biol. (0167-4412) 2010 orchard Only protein-protein interactions imex curation Involvement of Arabidopsis ROF2 (FKBP65) in thermotolerance. Involvement of Arabidopsis ROF2 (FKBP65) in thermotolerance. Plant Mol. Biol. (0167-4412) 2010 imex curation Meiri D., Tazat K., Cohen-Peer R., Farchi-Pisanty O., Aviezer-Hagai K., Avni A., Breiman A. adina@post.tau.ac.il 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions arath-leaf Arabidopsis thaliana leaf leaf leaf bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined Meiri D., Tazat K., Cohen-Peer R., Farchi-Pisanty O., Aviezer-Hagai K., Avni A., Breiman A. adina@post.tau.ac.il Accepted 2010-MAY-8 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Plant Mol. Biol. (0167-4412) 2010 orchard Only protein-protein interactions imex curation fkb65_arath Peptidyl-prolyl cis-trans isomerase FKBP65 FKBP65 70 kDa peptidyl-prolyl isomerase FK506-binding protein 65 Immunophilin FKBP65 Peptidyl-prolyl isomerase ROF2 Protein ROTAMASE FKBP 2 ROF2 Rotamase K15N18.12 At5g48570 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEDDFDTQNQFPEEEPEEMDMDLPDNDEADSAPYLKIGEEMEIGKSGLKKKLVKECEKWDTPENGDEVEVHYTGTLLDGTKFDSSRDRGTPFKFTLGQGHVIKGWDLGIKTMKKGENAIFTIPPELAYGETGSPPTIPPNATLQFDVELIAWRSVKDICGDGGVSKKIIVEGEKWEKPKDLDEVYVKYEARLEDGTIVGKSDGVEFTVKEGHFCPALSKAVKTMKRGEKVLLTVKPQYGFGEFGRPASDGLQAAIPPNATLQIDLELVSWKTVVEVTDDRKVIKKILKEGEGYERPNEGAIVKLKLIGKLQDGTTVFVKKGHEEDEEPFEFKIDEEQVIEGLEKAVMGMKKGEVALITISPEYAFGSSESKQELAVIPPNSTVYYEVELVSFIKEKESWDMNTQERIEAAGKKKEEGNVLFKAGKYARASKRYERGVKYIEYDSTFDEEEKKKSKDLKIACNLNDAACKLKLKDYKEAAKLSTKVLEMDSRNVKAMYRRAHAYLETADLDLAELDIKKALEIDPDNKEVKIEYKKLKEKVKEYNKKDAKFYSNMLSKMLEPHKGTQKEAQAMSIDTKA 0C56F57D22E37A3B fkb62_arath Peptidyl-prolyl cis-trans isomerase FKBP62 FKBP62 70 kDa peptidyl-prolyl isomerase FK506-binding protein 62 Immunophilin FKBP62 Peptidylprolyl isomerase ROF1 Protein ROTAMASE FKBP 1 ROF1 Rotamase MJL12.19 At3g25230 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDANFEMPPVGGMNDDDDMDFGDGASFLKVGEEKEIQQGLKKKLLKEGEGYETPENGDEVEVHYTGTLLDGTKFDSSRDRATPFKFTLGQGQVIKGWDIGIKTMKKGENAVFTIPAELAYGESGSPPTIPANATLQFDVELLKWDSVKDICKDGGVFKKILAVGEKWENPKDLDEVLVKFEAKLEDGTVVGKSDGVEFTVKDGHFCPALTKAVKTMKKGEKVLLTVKPQYGFGEKGKPASAGEGAVPPNATLEINLELVSWKTVSEVTDDNKVVKKVLKEGDGYERPNEGAVVKVKLIGKLQDGTVFLKKGHGENEEPFEFKTDEEQVVDGLDRAVMKMKKGEVALVTIDPEYAFGSNESQQELAVVPPNSTVTYEVDLLTFDKERESWDMNTEEKIEAASKKKEEGNSKFKGGKYSLASKRYEKAVKFIEYDTSFSEEEKKQAKALKVACNLNDAACKLKLKDYKQAEKLCTKVLELESTNVKALYRRAQAYMELSDLDLAEFDVKKALEIDPNNREVKLEQKRLKEKMKEFNKKEAKFYGNMFAKLSKE 36CF13637466D286 rof2-rof1-1 1 4 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Fig. 5B rof2-rof1-2 2 4 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component physical association physical association Fig. 5C rof2-rof1-3 3 5 unspecified role unspecified role unspecified role unspecified role region n-terminal of YFP tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role unspecified role unspecified role region c-terminal of YFP tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 6A