IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ WRM-1 activates the LIT-1 protein kinase to transduce anterior/posterior polarity signals in C. elegans. WRM-1 activates the LIT-1 protein kinase to transduce anterior/posterior polarity signals in C. elegans. Cell (0092-8674) 1999 imex curation Rocheleau CE., Yasuda J., Shin TH., Lin R., Sawa H., Okano H., Priess JR., Davis RJ., Mello CC. craig.mello@ummed.edu 23-Feb-2010: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Rocheleau CE., Yasuda J., Shin TH., Lin R., Sawa H., Okano H., Priess JR., Davis RJ., Mello CC. craig.mello@ummed.edu Accepted 2010-FEB-19 by ORCHARD. 23-Feb-2010: Contacted by JYOTI. Cell (0092-8674) 1999 orchard Only protein-protein interactions imex curation WRM-1 activates the LIT-1 protein kinase to transduce anterior/posterior polarity signals in C. elegans. WRM-1 activates the LIT-1 protein kinase to transduce anterior/posterior polarity signals in C. elegans. Cell (0092-8674) 1999 imex curation Rocheleau CE., Yasuda J., Shin TH., Lin R., Sawa H., Okano H., Priess JR., Davis RJ., Mello CC. craig.mello@ummed.edu 23-Feb-2010: Contacted by JYOTI. orchard Only protein-protein interactions caeel-embryo Caenorhabditis elegans embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti tag coip anti tag coimmunoprecipitation anti tag coip monoclonal western monoclonal antibody western blot monoclonal western Rocheleau CE., Yasuda J., Shin TH., Lin R., Sawa H., Okano H., Priess JR., Davis RJ., Mello CC. craig.mello@ummed.edu Accepted 2010-FEB-19 by ORCHARD. 23-Feb-2010: Contacted by JYOTI. Cell (0092-8674) 1999 orchard Only protein-protein interactions imex curation WRM-1 activates the LIT-1 protein kinase to transduce anterior/posterior polarity signals in C. elegans. WRM-1 activates the LIT-1 protein kinase to transduce anterior/posterior polarity signals in C. elegans. Cell (0092-8674) 1999 imex curation Rocheleau CE., Yasuda J., Shin TH., Lin R., Sawa H., Okano H., Priess JR., Davis RJ., Mello CC. craig.mello@ummed.edu 23-Feb-2010: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Rocheleau CE., Yasuda J., Shin TH., Lin R., Sawa H., Okano H., Priess JR., Davis RJ., Mello CC. craig.mello@ummed.edu Accepted 2010-FEB-19 by ORCHARD. 23-Feb-2010: Contacted by JYOTI. Cell (0092-8674) 1999 orchard Only protein-protein interactions imex curation WRM-1 activates the LIT-1 protein kinase to transduce anterior/posterior polarity signals in C. elegans. WRM-1 activates the LIT-1 protein kinase to transduce anterior/posterior polarity signals in C. elegans. Cell (0092-8674) 1999 imex curation Rocheleau CE., Yasuda J., Shin TH., Lin R., Sawa H., Okano H., Priess JR., Davis RJ., Mello CC. craig.mello@ummed.edu 23-Feb-2010: Contacted by JYOTI. orchard Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Rocheleau CE., Yasuda J., Shin TH., Lin R., Sawa H., Okano H., Priess JR., Davis RJ., Mello CC. craig.mello@ummed.edu Accepted 2010-FEB-19 by ORCHARD. 23-Feb-2010: Contacted by JYOTI. Cell (0092-8674) 1999 orchard Only protein-protein interactions imex curation nlk_caeel Serine/threonine kinase NLK nlk W06F12.1 lit-1 Nemo-like kinase Loss of intestine protein 1 protein protein caeel Caenorhabditis elegans MVSWGRGKDAYYLYISREQEEDDDDSLSFYSSQKDTEDEFCGCLFPDPEIPGSSSSSGCSSSSTELYDLAAAHAALISRQQQILSQAIPIIPEHQLAAVAAHHQHHQQLHPSVQYQLVAAATHHNHHQPQAAQPHYSAVVPRSDVIQQPPHFALHHHLQNLVQQQQQQQAHHHHQQLVGEMALVSHTHPAAVGSTTCYEKNQQKQQQVQQIPTQPQVAHVSSNAILAAAQPFYPPPVQDSQPDRPIGYGAFGVVWSVTDPRSGKRVALKKMPNVFQNLASCKRVFREIKMLSSFRHDNVLSLLDILQPANPSFFQELYVLTELMQSDLHKIIVSPQALTPDHVKVFVYQILRGLKYLHTANILHRDIKPGNLLVNSNCILKICDFGLARTWDQRDRLNMTHEVVTQYYRAPELLMGARRYTGAVDIWSVGCIFAELLQRKILFQAAGPIEQLQMIIDLLGTPSQEAMKYACEGAKNHVLRAGLRAPDTQRLYKIASPDDKNHEAVDLLQKLLHFDPDKRISVEEALQHRYLEEGRLRFHSCMCSCCYTKPNMPSRLFAQDLDPRHESPFDPKWEKDMSRLSMFELREKMYQFVMDRPALYGVALCINPQSAAYKNFASSSVAQASELPPSPQAW A9F2EA7E3E2CE7F5 wrm1_caeel Armadillo repeat-containing protein wrm-1 wrm-1 Worm armadillo protein 1 B0336.1 protein protein caeel Caenorhabditis elegans MDVDCAETFSQPCTPLNFNPMTPSTSRVSTPVRPSSTMSARQYSGSPFKAQPQNMEPSNSRVQELREAAVGKRSYTNAWMQGTYAPPQMAGQQQRFSRPPSVIGSTMSHMTNMSEMTAYSYGGLSMLSVNTEMGEFNNFVNQAPYQRALTRVSQVSENQDPTNRQYPMTAPEIIENLESTELINQAAAIRALEPIVKAGGMLQTWGPKGAEPIIRALFQVLIPRPVENENVIRKAFEILHHSILLSNKEIRRIDRMFFRLNAALMDPNGPPVFNVPKPYSIYEIVMTRAIQLDTKFESSAMVLLVHLCCKPHFMKIFFGEDETQQSPAHRRLHKIVIEFAIGNLRRPETKSKNKGLCVSIIKNLSNKNATIKDMSERLGVVSLFHQIMQNEVIHEDLLWSTMQALTVFCGDVKNGTHFVQMGGAQVLCGLLSHGSTRLLHELLKCLRRVSDLPAIQEQDMKESIHCIVQLIGCSDVTIVELATGTLRNIGLHNKMNKAFMVQDGVTSHAIAVLRTSEQFTYQPHANIDLYRKQILSIYENCLSVLNNVTSMAPQDIKESAVSACRMISENADSAYVLLHYFNVGNRKCRKLAVTVMKRVIETVPAFADPFVDLLGTTNEPLPILLLQRAFQSLDEWRKTSVEMMNCDGRSAEQRRELDDRRKDHEDIVKRSVGLLTNLCSQANPRFFHSLKLVLTNGTLNPFQWLTHEMSDGILQEWLAFILSICSRDESLQTFMMYRFLEQAKMTEAFFAELKARRQNSNIQTMLSKIIDLGRHQQRIVSQQHQQHQMQHHRQLM B517C7A804A7DEA8 pop1_caeel Protein pop-1 pop-1 W10C8.2 Posterior pharynx defect protein 1 protein protein caeel Caenorhabditis elegans MMADEELGDEVKVFRRDEDADDDPMISGETSEQQLADDKKEAVMEAELDGAGRNPSIDVLKSAFPKVEPMSPSFPGLMSHFSPGYSAAALPMFMPLFMNPYAAALRSPSLMFPMGAMSPTFPMFPPSPVYGAAIAAAAAKQHFENMAPLNMRAGHPMNQMGMPPYMHPSSMAPQNVDRRAQGGGKAKKDDHVKKPLNAFMWFMKENRKALLEEIGNNEKQSAELNKELGKRWHDLSKEEQAKYFEMAKKDKETHKERYPEWSARENYAVNKKKTKKRRDKSIPSENNDQKKCRARFGVNNTEMWCKFCKRKKKCEYATDRSGGSDITDSQDGRGTSGAYSSSSESPSPKANAGIALTTQQQQAAMMHTMLMQMRLGSTTGASTHVPSPLASSSAGRSPLDANASDSESDVEEEEDEQIDPTVMQQTHDMLMQESMCTI 494078BE40B40E2E wrm1-lit1-1 1 6 enzyme target enzyme target substrate substrate neutral component neutral component n terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 5 enzyme enzyme neutral component neutral component Q9U9Y8:p.Lys269Gly Alters kinetics of interaction mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K G Wed May 02 14:44:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys269gly' Tue Jun 30 19:06:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys269Gly' n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 phosphorylation phosphorylation reaction phosphorylation WRM-1 is both a substrate for and an activator of LIT-1-dependent kinase activity. Kinase activity requires the putative ATP-binding site in the LIT-1 kinase domain, as a point mutation at this position (K89G) abolished the phosphorylation of both LIT-1 and WRM-1. Fig 3A. wrm1-lit1-2 1 7 enzyme target enzyme target substrate substrate neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 enzyme enzyme neutral component neutral component n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 phosphorylation phosphorylation reaction phosphorylation For "Expressed in" for POP-1 GST: "bacterial" - cannot enter this. Ref 41 states "as previously desribed - with no referenece. (Presumably E.coli.) Fig 3B. A bacterially expressed GST-POP-1 protein can be phosphorylated by the LIT-1 immunoprecipitation complex and that phosphorylation required WRM-1 and an intact LIT-1 kinase domain. wrm1-lit1-3 3 6 unspecified role unspecified role prey prey n terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 enzyme enzyme bait bait physical association physical association Fig 4. wrm1-pop1-1 3 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Fig 4. POP-1 interacts weakly with full-length WRM-1. wrm1-lit1-5 4 6 unspecified role unspecified role bait bait n terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 enzyme enzyme prey prey n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 5 top image. wrm1-lit1-6 4 6 unspecified role unspecified role prey prey n terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 5 bottom image. wrm-1-lit1-7 2 5 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey physical association physical association Fig 5. wrm1-wrm-1 1 6 stimulator stimulator neutral component neutral component n terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 5 putative self putative self putative self putative self Q9U9Y8:p.Lys269Gly Alters kinetics of interaction mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K G Wed May 02 16:17:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys269gly' Tue Jun 30 17:01:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys269Gly' phosphorylation phosphorylation reaction phosphorylation WRM-1 is both a substrate for and an activator of LIT-1-dependent kinase activity. Fig 3A.