IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A protein-protein interaction map of the Trypanosoma brucei paraflagellar rod. A protein-protein interaction map of the Trypanosoma brucei paraflagellar rod. PLoS ONE 2009 imex curation Lacomble S., Portman N., Gull K. Interactions were ranked according to the number of assays in which they were observed; 4 interactions were detected in all four assays, 3 interactions were detected in three assays, one interaction in two assays and two interactions in one assay. keith.gull@path.ox.ac.uk 23-Feb-2010: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lacomble S., Portman N., Gull K. Interactions were ranked according to the number of assays in which they were observed; 4 interactions were detected in all four assays, 3 interactions were detected in three assays, one interaction in two assays and two interactions in one assay. keith.gull@path.ox.ac.uk Accepted 2010-FEB-6 by ORCHARD. 23-Feb-2010: Contacted by JYOTI. PLoS ONE 2009 orchard Only protein-protein interactions imex curation q382i7_tryb2 Tb11.01.5100 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MIEVQLNSFNLLPPEYPRRLVQEEENHRAVAALIELAETAIATAENYSGYVDSRLLPLSSRGFDLRSAASEMCERYKHEAPCGWTEEKVMRFCEQEVTREKIAELLSRQPLDVVAVESILSTLRDSTVEFPRGRFLLLRENLNILKPHQPRDIIRDLSELCGALYEIQFVDLLGKMREELAEFDPPLDAAKKKVQDAIEAHKRAVVGGDVLEVERTHRQLIAARYELVEVCAQRLKAFLSQNLENQEKGFIAELQALERDSLEALQQFTDYHSNQRDVIQEDIKKCSDKLLQEGATHEKCLEEYKVKEREMKDNLYAVISAKQKLVEEIQRKAAELVQLTAKQREIVDEQIKAKKEEERRITTYNEFANMGTQQKQRLLKCLEYCERVLSVVPGIKSYVGEMVKRLPWQKLRDARNEVNDTEAEGFMEAYDPFVECCGELTVKKMHRHDTLSRQSRLVEHNRNSSMESLDPNMNNYRAELEELIEQMKGVTGVINALNATQDAGEQLFLSVEKAILEKYERAATPFVHPLQVHGVKSVEERDRFVNRSMQYVEDEERKVLEKKSVLERMRRAVEGEETAVELAIRDIPVGRA AA69D6D9FB7ECF61 q586u8_tryb2 Tb927.2.4330 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MTQSAQSTTLFQRNKHIYIQEEDKLKDILSDEAFHEKVISWLESTITELDLQFEICDQKLAELQQNISVESGNYWCSKTVIKHCNLHLDVKNISSSGADTEQHATIPEVVHAVSSLQLLKTQPFITPKQKRFIEQCEAELSSVVFEPRELGFISNALRVKLREGSNARSLLGSLAEFQNTIEVLSPDVDKCEQLLEASISNGEMALAEEISERQLKIYERILQLITDQYPIITNHYVESRVNDRRQRWAIFRMADKDITAVIEGKNRQIEACEEDLLKIKEQIENYNNDDTHQRKRYETDRGLSDEFLQKNREQQQGVWNRMFELAREMQQCQVELSKLAQKRRKEIERRLQMEEREAGRRSGHESFLKAVSEHAQKLQNIVTNAIKAKDLATSLNNFVLDGCDTIASKVDKRQNTFSEMLRAIQSHHFKRYSDYYLAASRYLYRKERRLMQLDEEIRDNEIKKELHTESLDVNAKKYVEANKILLLKRRELGHQIVAIRANLDKASEDIEPTLSSLRFAGVEYVHPSEIAKKVNLNRHSTILDYREKVTPSTTFDEKVKVEEEKTLLQIRAAIEQENSERASYRRTQLPMLEAKYRTPLQRQVGALLDRRLNDTPSAGSPSASYPKAVTHPLPGHQKVPGTHGAGGEEPDRAAATLSAKPSHLVSSRRNSLEEMGDGVGGDASTASHGVQLGINDVLRVEGRSLRALYPYKARAPDELTFDEGEVIVCVSRAQEEGWLKGVCNQRTGLFPINYVAPCDEDD 9EA9DB5A357E5631 q57wb3_tryb2 EF-hand domain-containing protein Tb927.3.3770 Tb927.3.3790 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MSATEAVVEPKAPDVCTEANERPPTESGVAAPVKRLFDLLQEDSKERVVRLLTAFRELTLLEQESLAQEMGVTLPKEEKWRAPCPLIARQPSGPSLEVRRRDPSYVALCPSVSSALRELGSKEAARRLPDADLRKLVEGALGEDGVYLTVPESDGEGCAAASERVGVVDDGKPPLLVRSRCYQALRLARDEEIETDAQYTRPRVLNVTGIPLEESDVWEWFNLLDVARNGTVGVDAFLTSVRELDRGFGTSSKVQEAFEDESRSLSVDGQLSFDKFAYLVTRFSRY 2EF56E8167F27BAF q57xr7_tryb2 Tb927.7.6970 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MTKKSGHEGGTSLNGNGSSSTTPSNTVPTSLTFAGSVAATRKTFLQREAVLDACAVTTNNVALAQARRTALRDSGARIVKLFEELAQTYAANMKTATASQEGSGTGTPSAKDGPITVEQLNMINCMAEAYAESFRKECGLELALGDMVMSDAPANTSKKENDNSGSRNHNSASKGGDSTGGEAGGTAAGPAQEAIRRRIAPLQRALASAKELRNIPTYNKVLSVAQRGNVGANSLTTLVKNLTETLEESLQELEGGYGSCSNSANDVAEWACWVQPLVRLTNECLQTKPLEKIMEGPRERLRRVAFDVEEKQREQEDAVTDGDMVRSEQLYFEKTALLESMRPIYDELEAAIEESKRASVDEPSKELRSLVKELSTKQAPKVLDREKQVRRRGKTDLERLLARREEIMAARTKQTSTFKVYLAEWDKMFRHNEQQQENCLRAIEELEQRLKYLSEERAILVEDRLEVAAQEQQRSEDAASFMLFAAQHEQKLRKTIENLDQSLSCGERVLEVVRSSYHHLGKYLQDMVQREADEQLLEVRKERLSHFRGLYLTLGELKYKKERHLEELDKRIEYYHVQQELAMDTFNPKAKEFSKAKKDLLEVKETMQQQIDLIGQKAVKQLEDFKPTEQLLLASGVKFVHPVKELEEMNQRRTQKLLEYHNLMSTIGDGRSREEAEANGKVAPKTSS E05543CB0084CE1D q389k9_tryb2 Tb10.26.0680 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MSHYLLVTFTFNCPEIRILGPIKEQTIEKLNDVIPNATTTSRSNRTALPKFEYLPNPNHWYIKLDRQFCDEDGKSHLMVLLLDALSEEGSWRLVSSFVTKEPSGTGSKEGTETHTLFLNKLLAEDS 24D0CFB0051C0D67 q580g8_tryb2 Tb927.8.1550 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MSATYPERRPYATTVPHSGYLSKPTLPPVEALRILSSTVGIDLPRSVRHSANTLLRRSPTPRSVSPTRGVQLFKPPTLETATPFEFQEKDYHKSQQSVLNVQLSNAVEELFKVWRVKVRDLMHYLDSFPCEAGHSFSRTNGALSSFFEGYRTTDITPMTAHQLVNDFKLCTSVSMLDEIQNSPEVLGDQVRAALDALLNAKHFLAPLENFVDRLEPIAAFALPDKRPECLDGTRGPVWPSKVAVALHEKFHYDPSEYVLAELRKSDQPIERLENRVLQVKERKEDAIDREIPGDALNYLTTQVDLSNDLLLMNKARMELVYLHGKDIQEMKRMLEDLIVDARHSVELLKGRVERDMPLVKKDIDGVYQDVENTQKHIKDLDQKNAEANKAAHEAYSKLNDTEVELWVLLTDTIRKLVDIAGEKEAFVRNEMRLRELRGRDMAIAEELLAAQQSYFSRLRKSEDVLKRWENAAETYEAYVQAYVPTLLKKIHDAEEADKDLYNREAQNYVRRYEMFEYAVEEGRATRSVHVDRLQTLQRSKLLDVERALSTLDPFADKYRKELDEAQAQLDEGLAFIQLVENLAKERRGEVEPILQHVISYNRTLQTKTLEIEAEAEAQALLLRDGPATEGAPSTMNTVDAKVDTSLLQSARGSSGKRPATAAVSEVTHFCDAPPTSAALRLRSVVDANNMMCTVSHPHVMARTVGINHEEAFLEKYRRFTDEEQVAVEGVAGRVRQSNTSLEMLGEKYNNKDYIRMLLLSPGAKQSNSATEAAVEECA 4EC035CC8409629C q586b2_tryb2 Tb927.6.4140 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MPNLVVTATFSPPAFSIVGSPLREDTIKHMEERIPTVLSTALSRSREPSYFVHSKNPESWKMELSQHFCDELHRAAVSLVIIECLDAEGWVLRTSSSSQDTNEGKCCIKFYFTRS 290BFC71B0F8C4D6 q388r4_tryb2 Tb10.389.0100 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MDQSCNEAIQILQEVLTNADLRAVAAGGQTGDSETSTTPHLPMEKKLAAVCMLLREHSRVLLEQLCSINETASGGSLKSPQCNAANEFNDINGYALPRIVDTALFVRKSQLLRERVRRVNSTLGSVKHVGLLGDSSTGRRVITERAHAAVKELLDVVFTVASTLSVTPVLTFSTQGGEDVAPRAVKLPSDEFSAEPLLEHSELCRQTQDHIVTNYLICHEDSAINLPHRNEKGLSTVKEESNGYRSTTGKFRVAVLSKSFRGPLWQGLLLEENAIVDDIDGTRCTWKDIFAKDVGEAFGINAHSVWNIRALDVGTECGSGAVQRVQFHIRLPASVDDARAELLLRQCEFSGLLAQYTRLEEELESGGAGFASSPAVKDANNGHCELDDAPLNTHGNGIWNGDKLQRYNDINDESTLSTFPITLREEEAPSSGPPSPNEAFHYDPVGHTVRWPQQPPERESEVLGTGGEGPTLFQARLNLASSDAAPGNSVANFGVESHDADDASQAHGAGPLPNQGDDISNSPSLQPNSFSEDLLPISLFSSSQPQESCDVTKKQTSVLPQEQSINREWLSQLPPQPPQRRPYASQQDALQLLTTRHKKTFSGTDWDITVSRFEDDIIQTFSSEVTALFELSRECVKDVEFSHEDNSIVFGLVHDRYLLEDEINALIAVFDFPQVLKIHKRCLNHDKPTAVRFDCAAAHGAGGLHDRIDENNVVTTAPPNDLLSKEECCATPRGPNTSAQLFVDGGGSNPLEGEDECSDIAGIDWRQQQEFFLPSHEDSLTPDNEPATLTHIALLHDVPLELLIASNPHLATFDVDMPLPQSARLHIPRYGPLTETLSMSPSLRSQTPPSRRVKQEMNLRAVAMKLGVSVRELRQSNSHLDRYGDTELLPLSCSINVPALLVASADIGSFNSSQRSSPHSVVRATNRTLDQQLTTTWRGKDGTRYSSYQDHIPGAGEQPSESFPPKKPCSERTLARVESRSPSLLSRSESNKERRWHFTPWSEGGSPSARTAPRSSTARSIPPFIETAVRVMTRAVMKFLLLRFFAKWRFYARVKGHQAVKQPKTLYMQLKNPSRGEAQRLPTTGGSNRSQDVVAEKRSRPQVSDLRARLQELKRNSGTQSQPGAAPRSISKSVPRRDPSATREGKMVSPAVVTGGRGRKMTPVASAPVTNRTSEEGKQKDEYGRQGRANQRAPSAGSRLRRVSSEGRVSSPEKTQRGGMTKYPQRSRSLNNQLRFTSANPCSTGARSQSPSCEIRLKTGEPMMPEWIPIGLVISPERLTVEAQTAEASASGIERGDIIMEVDGKRVRTIRDVRSVLSSGLASHVFVTVRKRNKAIAAYRIPRSDGCR 76E9BF0908728B7A pfc3-pfc3 1 7 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association author score author-based confidence author score 4 1 The interactions of PFC3-PFR5 and PFC4-PFC16 reciprocate in both bait and prey configurations and both PFC3 and PFC6 interact with themselves. pfr5-pfc3 1 7 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association author score author-based confidence author score 3 1 The interactions of PFC3-PFR5 and PFC4-PFC16 reciprocate in both bait and prey configurations and both PFC3 and PFC6 interact with themselves. pfc3-pfr5 1 7 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey physical association physical association author score author-based confidence author score 3 1 The interactions of PFC3-PFR5 and PFC4-PFC16 reciprocate in both bait and prey configurations and both PFC3 and PFC6 interact with themselves. pfc4-pfc16 1 6 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association author score author-based confidence author score 3 1 The interactions of PFC3-PFR5 and PFC4-PFC16 reciprocate in both bait and prey configurations and both PFC3 and PFC6 interact with themselves. pfc16-pfc4 1 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association author score author-based confidence author score 4 1 The interactions of PFC3-PFR5 and PFC4-PFC16 reciprocate in both bait and prey configurations and both PFC3 and PFC6 interact with themselves. pfc6-pfc6 1 4 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association author score author-based confidence author score 4 1 The interactions of PFC3-PFR5 and PFC4-PFC16 reciprocate in both bait and prey configurations and both PFC3 and PFC6 interact with themselves. pfr5-pfc6 1 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association author score author-based confidence author score 1 1 8 non-redundant interactions were detected in the yeast two-hybrid screen (Figure 1) and these can be divided into two clusters: PAR1-PFC3-PFR5-PFC20-PFC6-PFR6 and PFC4-PFC16. In the first of these clusters, PFC3, PFC6 and PFC20 all interact with PFR5 while PFC3 also interacts with PAR1. pfr6-pfc6 1 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association author score author-based confidence author score 1 1 8 non-redundant interactions were detected in the yeast two-hybrid screen (Figure 1) and these can be divided into two clusters: PAR1-PFC3-PFR5-PFC20-PFC6-PFR6 and PFC4-PFC16. In the first of these clusters, PFC3, PFC6 and PFC20 all interact with PFR5 while PFC3 also interacts with PAR1. pfc3-par1 1 7 unspecified role unspecified role bait bait 2 unspecified role unspecified role prey prey physical association physical association author score author-based confidence author score 4 1 8 non-redundant interactions were detected in the yeast two-hybrid screen (Figure 1) and these can be divided into two clusters: PAR1-PFC3-PFR5-PFC20-PFC6-PFR6 and PFC4-PFC16. In the first of these clusters, PFC3, PFC6 and PFC20 all interact with PFR5 while PFC3 also interacts with PAR1. pfc20-pfr5 1 3 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association author score author-based confidence author score 2 1 8 non-redundant interactions were detected in the yeast two-hybrid screen (Figure 1) and these can be divided into two clusters: PAR1-PFC3-PFR5-PFC20-PFC6-PFR6 and PFC4-PFC16. In the first of these clusters, PFC3, PFC6 and PFC20 all interact with PFR5 while PFC3 also interacts with PAR1.