IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structural insight into processive human mitochondrial DNA synthesis and disease-related polymerase mutations. Structural insight into processive human mitochondrial DNA synthesis and disease-related polymerase mutations. Cell (0092-8674) 2009 imex curation Lee YS., Kennedy WD., Yin YW. I have not added the polymerase or footprinting data as in neither case could I find the sequence of the DNA used Superdex 200 gel filtration column whitney.yin@mail.utexas.edu 23-Dec-2009: Contacted by JYOTI. jyoti Only protein-protein interactions 2 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Lee YS., Kennedy WD., Yin YW. I have not added the polymerase or footprinting data as in neither case could I find the sequence of the DNA used Superdex 200 gel filtration column whitney.yin@mail.utexas.edu Accepted 2009-NOV-23 by JYOTI. 23-Dec-2009: Contacted by JYOTI. Cell (0092-8674) 2009 jyoti Only protein-protein interactions 2 imex curation Structural insight into processive human mitochondrial DNA synthesis and disease-related polymerase mutations. Structural insight into processive human mitochondrial DNA synthesis and disease-related polymerase mutations. Cell (0092-8674) 2009 imex curation Lee YS., Kennedy WD., Yin YW. I have not added the polymerase or footprinting data as in neither case could I find the sequence of the DNA used Superdex 200 gel filtration column whitney.yin@mail.utexas.edu 23-Dec-2009: Contacted by JYOTI. jyoti Only protein-protein interactions 2 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lee YS., Kennedy WD., Yin YW. I have not added the polymerase or footprinting data as in neither case could I find the sequence of the DNA used Superdex 200 gel filtration column whitney.yin@mail.utexas.edu Accepted 2009-NOV-23 by JYOTI. 23-Dec-2009: Contacted by JYOTI. Cell (0092-8674) 2009 jyoti Only protein-protein interactions 2 imex curation dpog1_human DNA polymerase subunit gamma-1 Mitochondrial DNA polymerase catalytic subunit PolG-alpha POLG MDP1 POLG1 POLGA protein protein human Homo sapiens Human MSRLLWRKVAGATVGPGPVPAPGRWVSSSVPASDPSDGQRRRQQQQQQQQQQQQQPQQPQVLSSEGGQLRHNPLDIQMLSRGLHEQIFGQGGEMPGEAAVRRSVEHLQKHGLWGQPAVPLPDVELRLPPLYGDNLDQHFRLLAQKQSLPYLEAANLLLQAQLPPKPPAWAWAEGWTRYGPEGEAVPVAIPEERALVFDVEVCLAEGTCPTLAVAISPSAWYSWCSQRLVEERYSWTSQLSPADLIPLEVPTGASSPTQRDWQEQLVVGHNVSFDRAHIREQYLIQGSRMRFLDTMSMHMAISGLSSFQRSLWIAAKQGKHKVQPPTKQGQKSQRKARRGPAISSWDWLDISSVNSLAEVHRLYVGGPPLEKEPRELFVKGTMKDIRENFQDLMQYCAQDVWATHEVFQQQLPLFLERCPHPVTLAGMLEMGVSYLPVNQNWERYLAEAQGTYEELQREMKKSLMDLANDACQLLSGERYKEDPWLWDLEWDLQEFKQKKAKKVKKEPATASKLPIEGAGAPGDPMDQEDLGPCSEEEEFQQDVMARACLQKLKGTTELLPKRPQHLPGHPGWYRKLCPRLDDPAWTPGPSLLSLQMRVTPKLMALTWDGFPLHYSERHGWGYLVPGRRDNLAKLPTGTTLESAGVVCPYRAIESLYRKHCLEQGKQQLMPQEAGLAEEFLLTDNSAIWQTVEELDYLEVEAEAKMENLRAAVPGQPLALTARGGPKDTQPSYHHGNGPYNDVDIPGCWFFKLPHKDGNSCNVGSPFAKDFLPKMEDGTLQAGPGGASGPRALEINKMISFWRNAHKRISSQMVVWLPRSALPRAVIRHPDYDEEGLYGAILPQVVTAGTITRRAVEPTWLTASNARPDRVGSELKAMVQAPPGYTLVGADVDSQELWIAAVLGDAHFAGMHGCTAFGWMTLQGRKSRGTDLHSKTATTVGISREHAKIFNYGRIYGAGQPFAERLLMQFNHRLTQQEAAEKAQQMYAATKGLRWYRLSDEGEWLVRELNLPVDRTEGGWISLQDLRKVQRETARKSQWKKWEVVAERAWKGGTESEMFNKLESIATSDIPRTPVLGCCISRALEPSAVQEEFMTSRVNWVVQSSAVDYLHLMLVAMKWLFEEFAIDGRFCISIHDEVRYLVREEDRYRAALALQITNLLTRCMFAYKLGLNDLPQSVAFFSAVDIDRCLRKEVTMDCKTPSNPTGMERRYGIPQGEALDIYQIIELTKGSLEKRSQPGP 2D9ECCD75AD6E01E dpog2_human DNA polymerase subunit gamma-2, mitochondrial Mitochondrial DNA polymerase accessory subunit PolG-beta MtPolB DNA polymerase gamma accessory 55 kDa subunit POLG2 MTPOLB protein protein human Homo sapiens Human MRSRVAVRACHKVCRCLLSGFGGRVDAGQPELLTERSSPKGGHVKSHAELEGNGEHPEAPGSGEGSEALLEICQRRHFLSGSKQQLSRDSLLSGCHPGFGPLGVELRKNLAAEWWTSVVVFREQVFPVDALHHKPGPLLPGDSAFRLVSAETLREILQDKELSKEQLVAFLENVLKTSGKLRENLLHGALEHYVNCLDLVNKRLPYGLAQIGVCFHPVFDTKQIRNGVKSIGEKTEASLVWFTPPRTSNQWLDFWLRHRLQWWRKFAMSPSNFSSSDCQDEEGRKGNKLYYNFPWGKELIETLWNLGDHELLHMYPGNVSKLHGRDGRKNVVPCVLSVNGDLDRGMLAYLYDSFQLTENSFTRKKNLHRKVLKLHPCLAPIKVALDVGRGPTLELRQVCQGLFNELLENGISVWPGYLETMQSSLEQLYSKYDEMSILFTVLVTETTLENGLIHLRSRDTTMKEMMHISKLKDFLIKYISSAKNV B99734BFEA249192 polg-polg2-7 2 3 unspecified role unspecified role neutral component neutral component P54098:p.Asp198Ala mutation mutation certain certain sequence position certain certain certain sequence position certain D A Wed May 02 20:12:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp198ala' Tue Jun 30 16:57:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp198Ala' P54098:p.Glu200Ala mutation mutation certain certain sequence position certain certain certain sequence position certain E A Wed May 02 19:58:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu200ala' Tue Jun 30 15:56:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu200Ala' c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 4 unspecified role unspecified role neutral component neutral component region LVSAETLREILQDKELSKEQLVAFLENVLKTSG -> GG experimental feature experimental feature substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Rfree=30.3%, Rworking=28.4%. 3.2A An exonuclease-deficient mutant of Pol γA was crystallized with the deletion mutant Pol γB-ΔI4, Table 1. The PDB accession numbers given at the end of the paper for this crystal appear to be incorrect. polg-polg2-8 1 4 unspecified role unspecified role neutral component neutral component region LVSAETLREILQDKELSKEQLVAFLENVLKTSG -> GG experimental feature experimental feature substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain P54098:p.Asp198Ala mutation mutation certain certain sequence position certain certain certain sequence position certain D A Wed May 02 17:41:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp198ala' Tue Jun 30 16:33:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp198Ala' P54098:p.Glu200Ala mutation mutation certain certain sequence position certain certain certain sequence position certain E A Wed May 02 15:03:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu200ala' Tue Jun 30 19:53:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu200Ala' spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Holoenzyme was formed by mixing Pol γΑ and Pol γΒ−ΔΙ4 monomer at a 1:2 molar ratio. Elution profiles of purified Pol γΑ (135 kDa; red curve) and Pol γΒ−ΔΙ4 dimer (100 kDa; blue curve) on a Superdex 200 gel filtration column are superimposed on that of the holoenzyme (235 kDa; purple curve). Supp. Fig. 2