MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Systematic and single cell analysis of Xenopus Piwi-interacting RNAs and Xiwi. Systematic and single cell analysis of Xenopus Piwi-interacting RNAs and Xiwi. EMBO J. (0261-4189) 2009 imex curation Lau NC., Ohsumi T., Borowsky M., Kingston RE., Blower MD. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Lau NC., Ohsumi T., Borowsky M., Kingston RE., Blower MD. EMBO J. (0261-4189) 2009 Only protein-protein interactions imex curation Systematic and single cell analysis of Xenopus Piwi-interacting RNAs and Xiwi. Systematic and single cell analysis of Xenopus Piwi-interacting RNAs and Xiwi. EMBO J. (0261-4189) 2009 imex curation Lau NC., Ohsumi T., Borowsky M., Kingston RE., Blower MD. Only protein-protein interactions xenla- Xenopus laevis egg egg anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Lau NC., Ohsumi T., Borowsky M., Kingston RE., Blower MD. EMBO J. (0261-4189) 2009 Only protein-protein interactions imex curation Systematic and single cell analysis of Xenopus Piwi-interacting RNAs and Xiwi. Systematic and single cell analysis of Xenopus Piwi-interacting RNAs and Xiwi. EMBO J. (0261-4189) 2009 imex curation Lau NC., Ohsumi T., Borowsky M., Kingston RE., Blower MD. Only protein-protein interactions xenla- Xenopus laevis egg egg fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Lau NC., Ohsumi T., Borowsky M., Kingston RE., Blower MD. EMBO J. (0261-4189) 2009 Only protein-protein interactions imex curation l14aa_xenla Protein LSM14 homolog A-A lsm14a-a lsm14a rap55a-a RNA-associated protein 55A-A protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MSGGTPYIGSKISLISKAEIRYEGILYTIDTENSTVALAKVRSFGTEDRPTDRPIPPRDEIFEYIIFRGSDIKDLTVCEPPKPQCSLPQDPAIVQSSLGSSSASSFQSVSSYGPFGRMPAYSQFNTGPLVGPQFGAVGVGSSLTSFGAETTSSTSLPPSSAVGTSFTQEARTLKTQSSQGQSSSPLDSLRKSPNIEQAVQTAAAPHAPSTATVGRRSPVLSRPVPSSIQKTAESPEQRKGELHKMQRPDIDQLKNDKNDPSKRQPVLSALQPRRGRGGNRGGRGRFGVRRDGPMKFEKDFDFESANAQFNKEEIDREFHNKLKIKDDKPEKPVNGEDKTDSVVDTQNSEGNAEEEEVLAGGVCYYDKTKSFFDNISCDDNRDRRQTWAEERRINVETFGLPLRSNRGRGGFRGRGGGMGFRGGRGRGGERRGAPGGGGFGPARGFRGGFRGGRGGREFADYEYRKDNKVAA 67A85C7E2FE93398 q2tav5_xenla ilf3.S CBTF122 ilf3 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MRPMRIFLNDDRHVMAKHSVVYPTQEELEAVQNMVSHTERALKAVSDWIDQQEKDCSGEQEQPMAEETETTEEGKDSEMKTGENPTRTLRGVMRVGLVAKGLLLKGDLDLELVLLCRDKPTISLLKRVADNLVLQFETVSEDKYEVIQNIREALIVVKSTKEPPLTLNIRLTSPLVREEMEKLSAGETLTVSDPPDVLDRHKCLAALASLRHAKWFQARANGLKSCVIVIRVLRDLCTRVPTWEPLRGWPLELLCEKAIGTANRPMGAGEALRRVLECLSSGILMPDGSGLYDPCEKDASDALEHLERQQREDITQSAQHALRLAAFGQLHKVLGMDPLPSKMPKKTKVETPVIDYTVQIPPSTTYAMPPLKRPIEEDGDDKSPSKKKKKIQKKDEKSEPPQVMNALMRLNQLKPGLQYKLISQTGPVHAPVFTMSVEVDDKTFEASGPSKKTAKLHVAVKVLQDMGLPTGMEEKEEGTDESEQKPVVQTPAQPDDSAEVDSAALDQAESAKQQGPILTKHGKNPVMELNEKRRGLKYELISETGGSHDKRFVMEVEVDGVKFQGSGSNKKVAKAYAALSALEKLFPDYTMYTEAPKKKRPPMMPRGGPKFAGKHNQGFGMMYSEVPPPQAMRGRGRGGMNRGRGRGRGGFGGGYMNSGGYGGGYGGNYNYQTSATAGYSQFYSNGGASGNAGGGGAGTGGYSSYYQGDSYSAPTPPKPFVNKKPPPPQQQQQQQPPPQHASNPPKPSYNQGYQGHQGGQQQQQQQQQQQTYNQNQYSNYGPPQKQKGGYNQGAQGAGSGGSYNYSNSYTGGTAPGYGSGEGAGGRGGSSYTAPSSGYNTGAHSGYGGASSTSTYQGYTQSNYNQGPPGQNYSGPPNNYQQPQGGAGSYSRNTEHNMSYQYR mint 3B1EE6B4DF384E51 epaba_xenla Embryonic polyadenylate-binding protein A epabp-a XePABP-A epab protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MNATGAGYPLASLYIGDLHPDVTEAMLYEKFSPAGPIMSIRVCRDIATRRSLSYAYINFQQPADAERALDTMNFEVIKGRPIRIMWSQRDPGLRKSGVGNVFIKNLDESIDNKALYDTFSAFGNILSCKVVCDEHGSRGYGFVHFETHEAANRAIQTMNGMLLNDRKVFVGHFKSRRERELEYGAKVMEFTNVYIKNFGEDMDDKRLREIFSAFGNTLSVKVMMDDSGRSRGFGFVNYGNHEEAQKAVSEMNGKEVNGRMIYVGRAQKRIERQSELKRKFEQIKQERINRYQGVNLYVKNLDDGIDDDRLRKEFLPYGTITSAKVMTEGGHSKGFGFVCFSSPEEATKAVTEMNGRIVSTKPLYVALAQRKEERKAILTNQYMQRLATMRAMPGPLLGSFQQPANYFLSAMPQPPNRTFYSPNPVAPVRPAPQWASHQSRPPQYQPPTPLMRAVQPRRMSSNISTMKQASTQVPRVAQHSQRVANIGTQTAGARAQVNPSMMRTMPHYKYSCGVRNVQPIVSSTHLQQVMEPAVLMQGQEPLTASLLAGAPPQEQKQMLGERIYPVIHEMHPTLAGKITGMLLEIDNSELLHMLESPESLHSKVEEAVAVLQAHQAKENAQKSAQPSLI mint F9C1BBB5A3E1B3AF piwl2_xentr Piwi-like protein 2 piwil2 protein protein xentr Xenopus tropicalis (Western clawed frog) Silurana tropicalis MDPTRPPFRGSPFHTPLGVRPPVLETKEEGPHGRAVLLPRGRALLGASAPSSDTTQRDPSDSRNVLPALFRGMGIETKPSGIPGRGGPVFGRGFLSTMSSGDGPDQPLSEPSIRPSLSTRVQQASDFSTERVALGRARFPIPPVSMEKPHVPTGRGLLFPSVLPTLTSDPVPKESPIATLQIEKEEKWEPLPKKGSKGSPCQLGLNLIKINFQNEAVYQYHVTFTPIVECRSMRFGMMKDHRSVTGPVTAFDGSILYLPVKLAQTVELESERRTDGQKIKITIQMTKILDPSSDLCLPFYNVVMRRVFKILDLKLVGRNFYDPASSTVLQQYRLQVWPGYAANIRKTDGGLFLLVDITHKIIRSDSVLDIMNILYQQSPENFQDEVTKQLVGSIVITRYNNRTYRIDDIEWNMSPKDSFSMSDGSKISFIDYYSKNYGITVKELDQPLLLHRPSERKAPKGKALDIVLLLPELAFMTGIPEKMRKDFRAMKDLTQQIHLSPKQHHISLGKLLKRIESSADAKNELQRWGLFLDTDIHMTTGRILPIEKINLRNNSFPAGEDLNWNREVTREACRSSVHLLYWAMIYPKRASAQAQELSSMLERIGGPIGIRVNHPNCVELRDDRVETYARSIKSLLEGEGKVQLLVCLISGTRDDLYGAIKKLCCVQNPVPSQVINTRTISQPQKLRSIAQKILLQINCKLGGELWGVDIPLKSVMVIGMDVYHDPSRGMRSVLGFVASINSCLTAWYSRVVFQLPNQEIMDSLKLCLVAALQKFFEVNHSLPEKIVVYRDGVSDGQLNTVENYEIPQLQTCFQTFDNYNPRMVVIVVQKRVSTNLYSTATGQFLTPQPGTVIDHTVTNRKWIDFFLMSHHVRQGCGIPTHYICVMNTANLGPDHLQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGQFLHHEPSIKLCDKLFFL mint 2257DC7720E3D672 q6ip60_xenla 60 kDa chaperonin hspd1.S 60 kDa heat shock protein, mitochondrial Chaperonin 60 Heat shock protein 60 Hspd1 hspd1 XELAEV_18047428mg protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MLRLQRILQHAKPASRVLALSLSRQYAKDVKFGAEARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKAIELKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDEEIGKIISDAMKKVGRKGVITVKDGKTLHDELEIIEGMKFDRGYISPYFINTAKGQKCEFQDAYLLLSEKKISSVQSIVPALEIANSHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAVSSGGVVFGEEGLSLSLEDIQPHDFGKVGEVIVTKDDTMILKGKGDQAQIEKRIQEIHDQLETTSSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPALDSLNPANEDQKVGIEIIRRTLKIPAMTIAKNAGVEGSLVVEKIIQSPVEIGYDAMNAEFVNLVEKGIIDPTKVVRTALMDAAGVASLLTTAEAVVTEIPKEEKDGGMAGMGGMGGMGGMGGGMGGGMF mint 897EDDF232C2B34B sympk_xenla Symplekin sympk protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MAVSMASQFFSQEEGTSEVVSTSERVVELLNMAALATNDTKITMLKQVQELIINKDPTLLDNFLDEIIAFQADKSVEVRKIVVNFIEEACKLDNELLIKLIANLHMLLKDENVNVVKKSILTMTQLYKVALQWVARSRPPSEQQESCWDLVTEMAADILLLLESDNDGMRTHAVKFVESLIVTLSPRTADSEVPKRQDGDISLEQIPPDHQFLKYNTLRDLGKQAVKELLKFMAHPAISSINLTAALGSLASIARQRPMFMAEVIQAYETLHANLPPTLAKSQVSSVRKNLKLQLLSVLRHPSSYEYQGPISTLLLDLGTSQGEITRHLPPPRDSNRKRAREDGEGGQKKSRTDIPLPDDDDDKDTMQLPTIPAPAPSTQSATDITADFVRPLLSPENVANLVLISMVYLPDSMPASFQSTYTPVESAGTDSQIRHLARLMATQMSAAGIGPGVEQSKDIPVDVVEEENIKPEGLVIKRRISALTKGQAISVLGCQSEKTKEEEEEEDDAPLAKKRPEPILPATQIRPSGVGGRKKVFRLKEVTEPLTDVQIKSLKLGAVRRILQAERSVGSSGASQMRVKVLARLVTQLDISVKAEVLSHFLNDPRMRLDLALAWLYQEYCEYQSGAGEEGYQECLIGILTGLQERPDQRDGVFTKVVLEAPLLTDSALDVLRKYCEDEGRSYLGMSTLRDLILTRPARQFQYLHLLLDLSSHEKDKIRQQSLHFIKRMYEKESLRPYIEKFALNYLQLLVHPNPPSVLFGADKDTEVAAPWTEDTVKQCLYLYLALLPQNHKLIHELASVYTEATADIKRTVLRVIETPIRGMGMNSPELLLLVENCPKGAETLVTRCLHILTDKVPPSPELVKRVRELYHKRLPDVRFLIPVLNGLDKKEVIQALPKLIKLNPIVVKEVFNRLLGTQHGEVSSSLSPLTPGDLLVALHNIDSSKCDMKSVIKATNLCFSARSVYTSEVLAVVLQQLMDTTPLPMLLMRTVIQALGMYPRLGGFIMNILTRLIYKQVWKYPKVWEGFIKCCQRTKPQSFSVLLQLPPPQLLSVLQTSPDLRDPLLAHVRAFTPHQLAHVPHSIMAILEAESRTDAGAEEAAQVEEEQETPAQDIIARRLAQEKSLKKQIEEQKGRNNQEMEEVAEVDPPAIFIAEEEEEESLKSQGKEEPQEDSSSNATTAEKSPVADEPMEEEPVEKKD mint C0E5839897716276 if23a_xenla Insulin-like growth factor 2 mRNA-binding protein 3-A KH domain-containing transcription factor B3-A Vg1 localization element binding protein A Vg1 RNA-binding protein A RNA-binding protein Vera-A VLE-binding protein A VgLE-binding and ER association protein A 69 kDa RNA-binding protein A Trans-acting factor B3-A VICKZ family member 3-A IGF-II mRNA-binding protein 3-A igf2bp3-a vera-a vickz3-a protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MNKLYIGNLSENVSPTDLESLFKESKIPFTGQFLVKSGYAFVDCPDETWAMKAIDTLSGKVELHGKVIEVEHSVPKRQRSRKLQIRNIPPHLQWEVLDSLLAQYGTVENCEQVNTESETAVVNVTYANKEHARQGLEKLNGYQLENYSLKVTYIPDEMATPQAPSQQLQQQPQQQHPQGRRGFGQRGPARQGSPGAAARPKPQTEVPLRMLVPTQFVGAIIGKEGATIRNITKQTQSKIDIHRKENAGAAEKPITIHSTPEGCSAACKIIMEIMQKEAQDTKFTEEIPLKILAHNNFVGRLIGKEGRNLKKIEQDTDTKITISPLQDLTLYNPERTITVKGSIEPCAKAEEEIMKKIRESYENDIAAMNLQAHLIPGLNLNALGLFPSSSSGMPPPSVGVPSPTSSTSYPPFGQQPESETVHLFIPALAVGAIIGKQGQHIKQLSRFAGASIKIAPAEGPDAKLRMVIITGPPEAQFKAQGRIYGKLKEENFFGPKEEVKLETHIKVPSYAAGRVIGKGGKTVNELQNLTSAEVVVPRDQTPDENDEVVVKITGHFYASQLAQRKIQEILAQVRRQQQQQQKTVQSGQPQPRRK 54CEA7BFF0856DD6 tdrd6_xenla Tudor domain-containing 6-like Xenopus tudor repeat xtr tdrd6 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MVEVYFIDHGNTEMVDWYNVKKLPAELREMPGLAIHCCVADICPLGVRWSPEAILAFKIAVVDKKLIIYVVSKELHKYIIEVLDNSRIEQRSMAKILSAAGHAKYEEVEPVAQHTGNMSDIDNETQQQFLGYINKDTSSLKTQQKEDVCSMEDDNSVPYSPYEDQFFEPGATIEVVVSCIISPGLFWCQNASLSSKLEKLMAKIQDYCSSTDCPYERGAYACLAKSSCDGKWYRAFITNNRPGSKANANQVEVLYVDYGITETVLVKDLRCIESELFDLKAQAFRCSLYNLIAPDSENPFEWDTKATLSFHRFVDSSAKKCSEFKCTFFATALVKTELSYIVDVFTPFASICKLLVELGHAKQLSHTTLAPSVQLQTYYYSMHDIKIGGEEEVYITYVNSSLEFYCQLSRNTETIDMIASATARVCSEVRKFELSVTPGPLCLAKFSDQQWYRCFINTNKNSTDAFFVDYGNTEKVNKEEMLPIPSDAYELLHFPMQAIKCSLSDMPDTVPSDVVLWFENHVLEKPLRAIIVAKETDGKLIVELYDGSQQINSILKTKLGWKSSRAEGSFGNSEKRNQLNDLDRGGRKETTSKFQPYSQGSKFSPDLDGHSQNGLTYQKPEFQTKEREQFEQKPNLRTPRSYNNDREVYQVQKNMSQSGFAPQKTGGFRSKDREAFEQRPNLKASGLYSQGRETPSMSQNSSYSGFPPQKTGAFRSKERQVSEHKQNSNPPKFYNQERKLSPQLRKASQNGSSSQTEAFWSSGSDQSSEHKPDNASQQRRSTFQESKLTPPLSKLSDLPKQNIALGMKSSVYVAHTNTISDFYVHIAQNTDLSNISEILNNEKGPSDQLDEKYVNLGDLICAFYEDGLYYRAVITEKCADGLLAQYIDYGNTSVIPPTKIYKLPPSLLSIPAMSICCALDKCTTSACEQNMDDLMLKFSERTGDLELSCEFVQYNNRKWNVILCDDQGCINDLFISVSGDPMLNPPLPKEPSVTSETLISASLFVWNLPELGETVEAFASAVDSPEHFWCQLATANVDSLAVKVQEAGEHSIHDGRFSAEIEVGSPCNVIYSDDNYWYRAAVTKMKDDKVTVRFVDYGNEETLQMEQVRRLPADIAAIPVQAFPCSLANFNLSEGCWSSEANTFFYDKVTEGLLEITVLHIQELGLCKIPQASVNVKYNGEDINCEMRRFWQDSFVNTNPFTESLNAKEETAIEDNVIPSQADEDDHSEPSEEPCASESIETPAVDGEVLTANDETKLEALPVSSAEEAAEITDNTDVELMRTEYLLHEVQKSSDLSCLELTLDEDVPDEKNSGTPAVTPLAAEDLCIDYDESNIKKSYSGVTTEMDNRELHQDEDLDLWTSAAQDQEIASSEILGDVPIDKECNYSVEEATDQSCTNIGLEEGPEPVENAFTENINDETDIANVQSKGEEEEAYLVPEESTVAECEIEDFEPEVDLQSKENEGLPDIPLLEGDGDDSVSPEEVSSHEMNEAEGLEDQDQELLGYTGTERAMDDYEVLQSEEQAEDLVPEEDPGTETEHRSYLFEAEEADLPSQEHKDFPEQEEDRVAEHKNDISEPDLQSKEQKEDLVPEEDPGTETEHRSYLFEAEETDLPSQEHKDTVTYTDIPLLEGGVDDFGSKETVSIDYNYEYVTEDVEDLDTENQESQICISGSDNRSKESGPVDLQDFEDEVLFQYTEPTADSASDVRQGDECEFAAHSDENIESPEHPVHTDSTADVCETDVCEPEVADHCHLQDKVVSERTECPVPDDRTKDDHQNNECQCAVDPVENIECQTPVCLVAADRSYTEYTWTVSETESGNMKNIEFQESPAEGDSVGSHGVGATEWKDGEPESLVNPDTPLLEGPVSVDIMHSSDNFEPETDDMEQMEQDQGRMKIESSYVPAPSV mint 275311110716F042 piwil2-epabp_a 2 vera\vg1rbp 10 ms participant Identification by mass spectrometry ms participant predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level RAP55 4 ms participant Identification by mass spectrometry ms participant predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level epab 6 ms participant Identification by mass spectrometry ms participant predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level xiwi 7 ms participant Identification by mass spectrometry ms participant predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level CBTF122 5 ms participant Identification by mass spectrometry ms participant predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level hsp60s 8 ms participant Identification by mass spectrometry ms participant predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association f5a mint piwil2-sympk 2 symplekin 9 ms participant Identification by mass spectrometry ms participant predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Xtr 11 ms participant Identification by mass spectrometry ms participant predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level xiwi 7 monoclonal western monoclonal antibody western blot monoclonal western predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association f5b mint IP after mild formaldehyde cross-linking (0,1%) sympk-piwil2 2 xiwi 7 monoclonal western monoclonal antibody western blot monoclonal western predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level symplekin 9 monoclonal western monoclonal antibody western blot monoclonal western predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association f5c mint tdrd6-piwil2 2 Xtr 11 monoclonal western monoclonal antibody western blot monoclonal western predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level xiwi 7 monoclonal western monoclonal antibody western blot monoclonal western predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association f5c mint piwil2-epabp_a-1 2 CBTF122 5 monoclonal western monoclonal antibody western blot monoclonal western predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level symplekin 9 monoclonal western monoclonal antibody western blot monoclonal western predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level xiwi 7 monoclonal western monoclonal antibody western blot monoclonal western predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Xtr 11 monoclonal western monoclonal antibody western blot monoclonal western predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level epab, 6 monoclonal western monoclonal antibody western blot monoclonal western predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association f5e mint To test whether some proteins are interacting with Xiwi through a shared interaction with mRNAs, we treated Xiwi IPs with RNaseA to digest associated RNAs and probed these IPs for ePAB, symplekin, CBTF122, and Xtr. We found that the interaction of Xiwi with ePAB, symplekin, and CBTF122 required RNA, whereas the Xiwi interaction with Xtr was RNA independent epabp_a-piwil2 1 epab 6 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified gfp tagged gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined xiwi 7 monoclonal western monoclonal antibody western blot monoclonal western predetermined predetermined participant predetermined unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association f5c mint piwil2-tdrd6 3 Xtr 11 immunostaining immunostaining predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell Xiwi 7 immunostaining immunostaining predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization f6 mint