IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Altered interactions within FY/AtCPSF complexes required for Arabidopsis FCA-mediated chromatin silencing. Altered interactions within FY/AtCPSF complexes required for Arabidopsis FCA-mediated chromatin silencing. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2009 Manzano D., Marquardt S., Jones AM., Baurle I., Liu F., Dean C. caroline.dean@bbsrc.ac.uk 01-Sep-2009: Contacted by JYOTI. orchard arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress tap tandem affinity purification TAP TAP tap de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Manzano D., Marquardt S., Jones AM., Baurle I., Liu F., Dean C. caroline.dean@bbsrc.ac.uk Accepted 2009-JUL-24 by ORCHARD. 01-Sep-2009: Contacted by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2009 orchard Altered interactions within FY/AtCPSF complexes required for Arabidopsis FCA-mediated chromatin silencing. Altered interactions within FY/AtCPSF complexes required for Arabidopsis FCA-mediated chromatin silencing. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2009 Manzano D., Marquardt S., Jones AM., Baurle I., Liu F., Dean C. caroline.dean@bbsrc.ac.uk 01-Sep-2009: Contacted by JYOTI. orchard arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Manzano D., Marquardt S., Jones AM., Baurle I., Liu F., Dean C. caroline.dean@bbsrc.ac.uk Accepted 2009-JUL-24 by ORCHARD. 01-Sep-2009: Contacted by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2009 orchard cpsf2_arath Cleavage and polyadenylation specificity factor subunit 2 CPSF100 MRO11.8 At5g23880 Cleavage and polyadenylation specificity factor 100 kDa subunit Protein EMBRYO DEFECTIVE 1265 EMB1265 ESP5 Protein ENHANCED SILENCING PHENOTYPE 5 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGTSVQVTPLCGVYNENPLSYLVSIDGFNFLIDCGWNDLFDTSLLEPLSRVASTIDAVLLSHPDTLHIGALPYAMKQLGLSAPVYATEPVHRLGLLTMYDQFLSRKQVSDFDLFTLDDIDSAFQNVIRLTYSQNYHLSGKGEGIVIAPHVAGHMLGGSIWRITKDGEDVIYAVDYNHRKERHLNGTVLQSFVRPAVLITDAYHALYTNQTARQQRDKEFLDTISKHLEVGGNVLLPVDTAGRVLELLLILEQHWSQRGFSFPIYFLTYVSSSTIDYVKSFLEWMSDSISKSFETSRDNAFLLRHVTLLINKTDLDNAPPGPKVVLASMASLEAGFAREIFVEWANDPRNLVLFTETGQFGTLARMLQSAPPPKFVKVTMSKRVPLAGEELIAYEEEQNRLKREEALRASLVKEEETKASHGSDDNSSEPMIIDTKTTHDVIGSHGPAYKDILIDGFVPPSSSVAPMFPYYDNTSEWDDFGEIINPDDYVIKDEDMDRGAMHNGGDVDGRLDEATASLMLDTRPSKVMSNELIVTVSCSLVKMDYEGRSDGRSIKSMIAHVSPLKLVLVHAIAEATEHLKQHCLNNICPHVYAPQIEETVDVTSDLCAYKVQLSEKLMSNVIFKKLGDSEVAWVDSEVGKTERDMRSLLPMPGAASPHKPVLVGDLKIADFKQFLSSKGVQVEFAGGGALRCGEYVTLRKVGPTGQKGGASGPQQILIEGPLCEDYYKIRDYLYSQFYLL DIP protein Q9LKF9 original sequence version: 108 9ABFC916712AD464 cpsf1_arath Cleavage and polyadenylation specificity factor subunit 1 CPSF160 Cleavage and polyadenylation specificity factor 160 kDa subunit K17N15.21 At5g51660 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSFAAYKMMHWPTGVENCASGYITHSLSDSTLQIPIVSVHDDIEAEWPNPKRGIGPLPNVVITAANILEVYIVRAQEEGNTQELRNPKLAKRGGVMDGVYGVSLELVCHYRLHGNVESIAVLPMGGGNSSKGRDSIILTFRDAKISVLEFDDSIHSLRMTSMHCFEGPDWLHLKRGRESFPRGPLVKVDPQGRCGGVLVYGLQMIILKTSQVGSGLVGDDDAFSSGGTVSARVESSYIINLRDLEMKHVKDFVFLHGYIEPVIVILQEEEHTWAGRVSWKHHTCVLSALSINSTLKQHPVIWSAINLPHDAYKLLAVPSPIGGVLVLCANTIHYHSQSASCALALNNYASSADSSQELPASNFSVELDAAHGTWISNDVALLSTKSGELLLLTLIYDGRAVQRLDLSKSKASVLASDITSVGNSLFFLGSRLGDSLLVQFSCRSGPAASLPGLRDEDEDIEGEGHQAKRLRMTSDTFQDTIGNEELSLFGSTPNNSDSAQKSFSFAVRDSLVNVGPVKDFAYGLRINADANATGVSKQSNYELVCCSGHGKNGALCVLRQSIRPEMITEVELPGCKGIWTVYHKSSRGHNADSSKMAADEDEYHAYLIISLEARTMVLETADLLTEVTESVDYYVQGRTIAAGNLFGRRRVIQVFEHGARILDGSFMNQELSFGASNSESNSGSESSTVSSVSIADPYVLLRMTDDSIRLLVGDPSTCTVSISSPSVLEGSKRKISACTLYHDKGPEPWLRKASTDAWLSSGVGEAVDSVDGGPQDQGDIYCVVCYESGALEIFDVPSFNCVFSVDKFASGRRHLSDMPIHELEYELNKNSEDNTSSKEIKNTRVVELAMQRWSGHHTRPFLFAVLADGTILCYHAYLFDGVDSTKAENSLSSENPAALNSSGSSKLRNLKFLRIPLDTSTREGTSDGVASQRITMFKNISGHQGFFLSGSRPGWCMLFRERLRFHSQLCDGSIAAFTVLHNVNCNHGFIYVTAQGVLKICQLPSASIYDNYWPVQKIPLKATPHQVTYYAEKNLYPLIVSYPVSKPLNQVLSSLVDQEAGQQLDNHNMSSDDLQRTYTVEEFEIQILEPERSGGPWETKAKIPMQTSEHALTVRVVTLLNASTGENETLLAVGTAYVQGEDVAARGRVLLFSFGKNGDNSQNVVTEVYSRELKGAISAVASIQGHLLISSGPKIILHKWNGTELNGVAFFDAPPLYVVSMNVVKSFILLGDVHKSIYFLSWKEQGSQLSLLAKDFESLDCFATEFLIDGSTLSLAVSDEQKNIQVFYYAPKMIESWKGLKLLSRAEFHVGAHVSKFLRLQMVSSGADKINRFALLFGTLDGSFGCIAPLDEVTFRRLQSLQKKLVDAVPHVAGLNPLAFRQFRSSGKARRSGPDSIVDCELLCHYEMLPLEEQLELAHQIGTTRYSILKDLVDLSVGTSFL DIP protein Q9FGR0 original sequence version: 89 14741C95CB4190BF fy_arath Flowering time control protein FY FY T6I14.10 At5g13480 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MYAGGDMHRGSQMPQPPMMRQSSASSTNINPDYHHPSGPFDPNVDSFGAKRMRKHTQRRAVDYTSTVVRYIQARTWQRDSRDRTTLQPTPAAAVDMLPTVAYSDNPSTSFAAKFVHASLNKNRCSINRVLWTPSGRRLITGSQSGEFTLWNGQSFNFEMILQAHDQPIRSMVWSHNENYMVSGDDGGTLKYWQNNMNNVKANKTAHKESIRDLSFCKTDLKFCSCSDDTTVKVWDFTKCVDESSLTGHGWDVKSVDWHPTKSLLVSGGKDQLVKLWDTRSGRELCSLHGHKNIVLSVKWNQNGNWLLTASKDQIIKLYDIRTMKELQSFRGHTKDVTSLAWHPCHEEYFVSGSSDGSICHWIVGHENPQIEIPNAHDNSVWDLAWHPIGYLLCSGSNDHTTKFWCRNRPADNPRDVLMQNQGYNEQGFGRQPDNFQPSEASPIPGAFVPGLTRNEGTIPGIGIAMPFDASSQGDHKQPLPGSMALGAPPLPPGPHPSLLGSGQQQGYQQQQQHQGHPQQMLPMPNMPHHQLPPSSHMPLHPHHLPRPMQMPPHGHMPPPSMPMSHQMPGSMGMQGGMNPQMSQSHFMGAPSGVFQGQPNSGGPQMYPQGRGGFNRPQMIPGYNNPFQQQQQPPLPPGPPPNNNQQHQ DIP protein Q6NLV4 original sequence version: 79 74569D2178BEA5C7 fy-cpsf100-cpsf160 1 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait c-terminal tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Figure 2C fy-cpsf160 2 5 unspecified role unspecified role neutral component neutral component c-terminal tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role neutral component neutral component physical association physical association Figure 3