IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ An effector of RNA-directed DNA methylation in arabidopsis is an ARGONAUTE 4- and RNA-binding protein. An effector of RNA-directed DNA methylation in arabidopsis is an ARGONAUTE 4- and RNA-binding protein. Cell (0092-8674) 2009 imex curation He XJ., Hsu YF., Zhu S., Wierzbicki AT., Pontes O., Pikaard CS., Liu HL., Wang CS., Jin H., Zhu JK. Plant extracts taken from Myc-AGO4 transgenic plants. jian-kang.zhu@ucr.edu 27-Oct-2007: Contacted by JYOTI. davet Only protein-protein interactions 8 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot He XJ., Hsu YF., Zhu S., Wierzbicki AT., Pontes O., Pikaard CS., Liu HL., Wang CS., Jin H., Zhu JK. Plant extracts taken from Myc-AGO4 transgenic plants. jian-kang.zhu@ucr.edu Accepted 2009-JUL-18 by DAVET. Cell (0092-8674) 2009 davet Only protein-protein interactions 8 imex curation An effector of RNA-directed DNA methylation in arabidopsis is an ARGONAUTE 4- and RNA-binding protein. An effector of RNA-directed DNA methylation in arabidopsis is an ARGONAUTE 4- and RNA-binding protein. Cell (0092-8674) 2009 imex curation He XJ., Hsu YF., Zhu S., Wierzbicki AT., Pontes O., Pikaard CS., Liu HL., Wang CS., Jin H., Zhu JK. Plant extracts taken from Myc-AGO4 transgenic plants. jian-kang.zhu@ucr.edu 27-Oct-2007: Contacted by JYOTI. davet Only protein-protein interactions 8 in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa predetermined predetermined participant predetermined He XJ., Hsu YF., Zhu S., Wierzbicki AT., Pontes O., Pikaard CS., Liu HL., Wang CS., Jin H., Zhu JK. Plant extracts taken from Myc-AGO4 transgenic plants. jian-kang.zhu@ucr.edu Accepted 2009-JUL-18 by DAVET. 27-Oct-2007: Contacted by JYOTI. Cell (0092-8674) 2009 davet Only protein-protein interactions 8 imex curation An effector of RNA-directed DNA methylation in arabidopsis is an ARGONAUTE 4- and RNA-binding protein. An effector of RNA-directed DNA methylation in arabidopsis is an ARGONAUTE 4- and RNA-binding protein. Cell (0092-8674) 2009 imex curation He XJ., Hsu YF., Zhu S., Wierzbicki AT., Pontes O., Pikaard CS., Liu HL., Wang CS., Jin H., Zhu JK. Plant extracts taken from Myc-AGO4 transgenic plants. jian-kang.zhu@ucr.edu 27-Oct-2007: Contacted by JYOTI. davet Only protein-protein interactions 8 arath-flower Arabidopsis thaliana flower flower Flower anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western He XJ., Hsu YF., Zhu S., Wierzbicki AT., Pontes O., Pikaard CS., Liu HL., Wang CS., Jin H., Zhu JK. Plant extracts taken from Myc-AGO4 transgenic plants. jian-kang.zhu@ucr.edu Accepted 2009-JUL-18 by DAVET. 27-Oct-2007: Contacted by JYOTI. Cell (0092-8674) 2009 davet Only protein-protein interactions 8 imex curation An effector of RNA-directed DNA methylation in arabidopsis is an ARGONAUTE 4- and RNA-binding protein. An effector of RNA-directed DNA methylation in arabidopsis is an ARGONAUTE 4- and RNA-binding protein. Cell (0092-8674) 2009 imex curation He XJ., Hsu YF., Zhu S., Wierzbicki AT., Pontes O., Pikaard CS., Liu HL., Wang CS., Jin H., Zhu JK. Plant extracts taken from Myc-AGO4 transgenic plants. jian-kang.zhu@ucr.edu 27-Oct-2007: Contacted by JYOTI. davet Only protein-protein interactions 8 arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining He XJ., Hsu YF., Zhu S., Wierzbicki AT., Pontes O., Pikaard CS., Liu HL., Wang CS., Jin H., Zhu JK. Plant extracts taken from Myc-AGO4 transgenic plants. jian-kang.zhu@ucr.edu Accepted 2009-JUL-18 by DAVET. 27-Oct-2007: Contacted by JYOTI. Cell (0092-8674) 2009 davet Only protein-protein interactions 8 imex curation An effector of RNA-directed DNA methylation in arabidopsis is an ARGONAUTE 4- and RNA-binding protein. An effector of RNA-directed DNA methylation in arabidopsis is an ARGONAUTE 4- and RNA-binding protein. Cell (0092-8674) 2009 imex curation He XJ., Hsu YF., Zhu S., Wierzbicki AT., Pontes O., Pikaard CS., Liu HL., Wang CS., Jin H., Zhu JK. Plant extracts taken from Myc-AGO4 transgenic plants. jian-kang.zhu@ucr.edu 27-Oct-2007: Contacted by JYOTI. davet Only protein-protein interactions 8 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western He XJ., Hsu YF., Zhu S., Wierzbicki AT., Pontes O., Pikaard CS., Liu HL., Wang CS., Jin H., Zhu JK. Plant extracts taken from Myc-AGO4 transgenic plants. jian-kang.zhu@ucr.edu Accepted 2009-JUL-18 by DAVET. 27-Oct-2007: Contacted by JYOTI. Cell (0092-8674) 2009 davet Only protein-protein interactions 8 imex curation An effector of RNA-directed DNA methylation in arabidopsis is an ARGONAUTE 4- and RNA-binding protein. An effector of RNA-directed DNA methylation in arabidopsis is an ARGONAUTE 4- and RNA-binding protein. Cell (0092-8674) 2009 imex curation He XJ., Hsu YF., Zhu S., Wierzbicki AT., Pontes O., Pikaard CS., Liu HL., Wang CS., Jin H., Zhu JK. Plant extracts taken from Myc-AGO4 transgenic plants. jian-kang.zhu@ucr.edu 27-Oct-2007: Contacted by JYOTI. davet Only protein-protein interactions 8 arath-flower Arabidopsis thaliana flower flower Flower anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot He XJ., Hsu YF., Zhu S., Wierzbicki AT., Pontes O., Pikaard CS., Liu HL., Wang CS., Jin H., Zhu JK. Plant extracts taken from Myc-AGO4 transgenic plants. jian-kang.zhu@ucr.edu Accepted 2009-JUL-18 by DAVET. 27-Oct-2007: Contacted by JYOTI. Cell (0092-8674) 2009 davet Only protein-protein interactions 8 imex curation rd29a_arath_prom_rna RNA corresponding to the Arabidopsis RD29A promoter region rna ribonucleic acid RNA rna chemical synthesis Chemical synthesis (Chemical synthesis) RNA corresponding to region described in EMBL record AY973635 ago4_arath Protein argonaute 4 At2g27040 AGO4 OCP11 Protein OVEREXPRESSOR OF CATIONIC PEROXIDASE 11 T20P8.9 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDSTNGNGADLESANGANGSGVTEALPPPPPVIPPNVEPVRVKTELAEKKGPVRVPMARKGFGTRGQKIPLLTNHFKVDVANLQGHFFHYSVALFYDDGRPVEQKGVGRKILDKVHQTYHSDLDGKEFAYDGEKTLFTYGALPSNKMDFSVVLEEVSATRANGNGSPNGNESPSDGDRKRLRRPNRSKNFRVEISYAAKIPLQALANAMRGQESENSQEAIRVLDIILRQHAARQGCLLVRQSFFHNDPTNCEPVGGNILGCRGFHSSFRTTQGGMSLNMDVTTTMIIKPGPVVDFLIANQNARDPYSIDWSKAKRTLKNLRVKVSPSGQEFKITGLSDKPCREQTFELKKRNPNENGEFETTEVTVADYFRDTRHIDLQYSADLPCINVGKPKRPTYIPLELCALVPLQRYTKALTTFQRSALVEKSRQKPQERMTVLSKALKVSNYDAEPLLRSCGISISSNFTQVEGRVLPAPKLKMGCGSETFPRNGRWNFNNKEFVEPTKIQRWVVVNFSARCNVRQVVDDLIKIGGSKGIEIASPFQVFEEGNQFRRAPPMIRVENMFKDIQSKLPGVPQFILCVLPDKKNSDLYGPWKKKNLTEFGIVTQCMAPTRQPNDQYLTNLLLKINAKLGGLNSMLSVERTPAFTVISKVPTIILGMDVSHGSPGQSDVPSIAAVVSSREWPLISKYRASVRTQPSKAEMIESLVKKNGTEDDGIIKELLVDFYTSSNKRKPEHIIIFRDGVSESQFNQVLNIELDQIIEACKLLDANWNPKFLLLVAQKNHHTKFFQPTSPENVPPGTIIDNKICHPKNNDFYLCAHAGMIGTTRPTHYHVLYDEIGFSADELQELVHSLSYVYQRSTSAISVVAPICYAHLAAAQLGTFMKFEDQSETSSSHGGITAPGPISVAQLPRLKDNVANSMFFC DIP protein Q9ZVD5 original sequence version: 48 C0323FAE9592B147 rdm3_arath Protein RNA-directed DNA methylation 3 KOW domain-containing transcription factor 1 KTF1 Protein SPT5-like RDM3 SPT5L T19N18.20 At5g04290 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDRKGKGKQVAGSDSYSGGQKRKNSVEFRDEGLRIKKRKNPEVLQFFEESAEVGYYGGSSDEDDDGLGFLNDMEDEPEVEESSKAGKGEKGKSSFVFPKEEDLNEEEFDRIMEERYKPGSGFLRYADDDIKDAIEMDALAPTSKDPPIWKVKCAIGRERRSVFCLMHKFVELRKIGTKLEIISVFSVDHVKGFIFIEADKEHDVLEACKSLVGIYATRMVLLPKAETPNLLTVQKKTKKVSEGTWARVKNGKYKGDLAQIVAVSDTRNKALIKLIPRIDIQALTQKYGGGVTVQKGQTPAPRLISSSELEEFRPLIQVRRDRDTGITFEHLDSLMLKDGYLYKKVSLDSISSWGVIPTKDELLKFTPVDRKETGDVEWISEIYGEERKKKILPTCREGGKGEGSGGGKGEGSGGGKGEGSRGGKGEGSSDFKSESSYELYNLVCFSRKDFGLIVGVDDKGDGYKVLKEGIDGPVVVTVGKKEMQNGPFDSKFTALDLNKKQISVNDVVKISKGPSEGKQGVVRQVYRGIIFLYDESEEENGGYFCCKSQSCEKVKLFTEESNEKTGGFDGTAFEDFVSSPKSPLSPEKEWQPRERYNSSNQGDIGSTYSIGQKLRIRVGPLKGYLCRVIALRYSDVTVKLDSQHKIFTVKSEHLAEVRDRNTVLSTSGDAGTGSFQPFGMLGTESSTGDWAIGAGTSSEGGNWNIGGPSTDSHESLNIERNMVQLCREKNPWGGSKPTSDVSPTVADDNTSAWANAAAENKPASASDQPGGWNPWGKTPASEAGTVSGWGDTSASNVEASSWEKQGASTSNVADLGSWGTHGGSSGGNKQDEDSVWGKLCEASESSQKKEESSWGKKGGSDGESSWGNKDGNSSASKKDGVSWGQQDKGSDESKGGSAWSNQCGDFGSGKKKDGSSGWNKSAEDSNANSKGVPDWGQPNDGSSWGKKGDGAASWGKKDDGGSWGKKDDGNKDDGGSSWGKKDDGQKDDGGSSWEKKFDGGSSWGKKDDGGSSWGKKDDGGSLWGKKDDGGSSWGKEDDGGSLWGKKDDGESSWGKKDDGESSWGKKDDGGSSWGKKDEGGYSEQTFDRGGRGFGGRRGGGRRGGRDQFGRGSSFGNSEDPAPWSKPSGGSSWGKQDGDGGGSSWGKENDAGGGSSWGKQDNGVGSSWGKQNDGSGGGSSWGKQNDAGGGSSWGKQDSGGDGSSWGKQDGGGDSGSAWGKQNNTSGGSSWGKQSDAGGGSSWGKQDGGGGGSSWGKQDGGGGSGSAWGKQNETSNGSSWGKQNDSGGGSSWGKQDGGGGGSSWGKQNDGGGGSSWGKQGDGGSKPWNEHSGGGRGFGERRGGGGFRGGRNQSGRGGRSFDGGRSSSWKTDNQENTWKSDQSGGSDWKKGWGEDSNNSKPSGSSAGGCAGNWPSWDTNSKKETNDKPGDDSKSAWGTSNDQVNTDNNNDSWNKKPNNDVGTSGEADNAWGGKTNAVAPSPSGSAAWGTGDKKTGW CBF42232AD22572D nrpe1_arath DNA-directed RNA polymerase V subunit 1 NRPE1 DMS5 At2g40030 DRD3 NRPD1b RMD1 RPE1 DNA-directed RNA polymerase D subunit 1b Protein DEFECTIVE IN MERISTEM SILENCING 5 Protein DEFECTIVE IN RNA-DIRECTED DNA METHYLATION 3 Protein RNA-DIRECTED DNA METHYLATION DEFECTIVE 1 RNA polymerase IV subunit 1 T28M21.19 DNA-directed RNA polymerase E subunit 1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEEESTSEILDGEIVGITFALASHHEICIQSISESAINHPSQLTNAFLGLPLEFGKCESCGATEPDKCEGHFGYIQLPVPIYHPAHVNELKQMLSLLCLKCLKIKKAKGTSGGLADRLLGVCCEEASQISIKDRASDGASYLELKLPSRSRLQPGCWNFLERYGYRYGSDYTRPLLAREVKEILRRIPEESRKKLTAKGHIPQEGYILEYLPVPPNCLSVPEASDGFSTMSVDPSRIELKDVLKKVIAIKSSRSGETNFESHKAEASEMFRVVDTYLQVRGTAKAARNIDMRYGVSKISDSSSSKAWTEKMRTLFIRKGSGFSSRSVITGDAYRHVNEVGIPIEIAQRITFEERVSVHNRGYLQKLVDDKLCLSYTQGSTTYSLRDGSKGHTELKPGQVVHRRVMDGDVVFINRPPTTHKHSLQALRVYVHEDNTVKINPLMCSPLSADFDGDCVHLFYPQSLSAKAEVMELFSVEKQLLSSHTGQLILQMGSDSLLSLRVMLERVFLDKATAQQLAMYGSLSLPPPALRKSSKSGPAWTVFQILQLAFPERLSCKGDRFLVDGSDLLKFDFGVDAMGSIINEIVTSIFLEKGPKETLGFFDSLQPLLMESLFAEGFSLSLEDLSMSRADMDVIHNLIIREISPMVSRLRLSYRDELQLENSIHKVKEVAANFMLKSYSIRNLIDIKSNSAITKLVQQTGFLGLQLSDKKKFYTKTLVEDMAIFCKRKYGRISSSGDFGIVKGCFFHGLDPYEEMAHSIAAREVIVRSSRGLAEPGTLFKNLMAVLRDIVITNDGTVRNTCSNSVIQFKYGVDSERGHQGLFEAGEPVGVLAATAMSNPAYKAVLDSSPNSNSSWELMKEVLLCKVNFQNTTNDRRVILYLNECHCGKRFCQENAACTVRNKLNKVSLKDTAVEFLVEYRKQPTISEIFGIDSCLHGHIHLNKTLLQDWNISMQDIHQKCEDVINSLGQKKKKKATDDFKRTSLSVSECCSFRDPCGSKGSDMPCLTFSYNATDPDLERTLDVLCNTVYPVLLEIVIKGDSRICSANIIWNSSDMTTWIRNRHASRRGEWVLDVTVEKSAVKQSGDAWRVVIDSCLSVLHLIDTKRSIPYSVKQVQELLGLSCAFEQAVQRLSASVRMVSKGVLKEHIILLANNMTCSGTMLGFNSGGYKALTRSLNIKAPFTEATLIAPRKCFEKAAEKCHTDSLSTVVGSCSWGKRVDVGTGSQFELLWNQKETGLDDKEETDVYSFLQMVISTTNADAFVSSPGFDVTEEEMAEWAESPERDSALGEPKFEDSADFQNLHDEGKPSGANWEKSSSWDNGCSGGSEWGVSKSTGGEANPESNWEKTTNVEKEDAWSSWNTRKDAQESSKSDSGGAWGIKTKDADADTTPNWETSPAPKDSIVPENNEPTSDVWGHKSVSDKSWDKKNWGTESAPAAWGSTDAAVWGSSDKKNSETESDAAAWGSRDKNNSDVGSGAGVLGPWNKKSSETESNGATWGSSDKTKSGAAAWNSWDKKNIETDSEPAAWGSQGKKNSETESGPAAWGAWDKKKSETEPGPAGWGMGDKKNSETELGPAAMGNWDKKKSDTKSGPAAWGSTDAAAWGSSDKNNSETESDAAAWGSRNKKTSEIESGAGAWGSWGQPSPTAEDKDTNEDDRNPWVSLKETKSREKDDKERSQWGNPAKKFPSSGGWSNGGGADWKGNRNHTPRPPRSEDNLAPMFTATRQRLDSFTSEEQELLSDVEPVMRTLRKIMHPSAYPDGDPISDDDKTFVLEKILNFHPQKETKLGSGVDFITVDKHTIFSDSRCFFVVSTDGAKQDFSYRKSLNNYLMKKYPDRAEEFIDKYFTKPRPSGNRDRNNQDATPPGEEQSQPPNQSIGNGGDDFQTQTQSQSPSQTRAQSPSQAQAQSPSQTQSQSQSQSQSQSQSQSQSQSQSQSQSQSQSQSQSPSQTQTQSPSQTQAQAQSPSSQSPSQTQT DIP protein Q5D869 original sequence version: 45 C16F6B0BBDC40617 ago4-ktf1-1 1 8 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined arath-flower Arabidopsis thaliana flower flower Flower 9 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association Myc-AGO4 was captured by all three versions of truncated KTF1 proteins from the C-terminal region Fig. 4D ago4-nrpe1-1 1 10 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined arath-flower Arabidopsis thaliana flower flower Flower physical association physical association Western blot analysis showing that the GST-fused truncated KTF1 and NRPE1-CTD interact with Myc-AGO4 from plant extracts. Fig. 4D The TAIR/UniProtKB NRPE1 protein transcript is longer (1976aa) than that described in the text (1874) therefore the c-terminal domain has not been accurately described as a feature. ago4-ktf1-2 5 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined arath-flower Arabidopsis thaliana flower flower Flower physical association physical association The result shows that GST-KTF1-P1 and GST-NRPE1-CTD, but not GST, were captured by the Myc antibodies (Figure 4E). Fig. 4E ago4-nrpe1-2 5 10 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined arath-flower Arabidopsis thaliana flower flower Flower physical association physical association The result shows that GST-KTF1-P1 and GST-NRPE1-CTD, but not GST, were captured by the Myc antibodies (Figure 4E). Fig. 4E The TAIR/UniProtKB NRPE1 protein transcript is longer (1976aa) than that described in the text (1874) therefore the c-terminal domain has not been accurately described as a feature. ago4-ktf1-3 6 8 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey physical association physical association Western blot analysis showing coimmunoprecipitation of KTF1 and Myc-AGO4. Fig. 4G ago4-ktf1-4 3 8 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait physical association physical association Western blot analysis showing coimmunoprecipitation of KTF1 and Myc-AGO4. Fig. 4F ago4-ktf1-5 4 9 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization we performed coimmunolocalization experiments using antibody against the native KTF1 protein and transgenic lines expressing NRPE1-Flag and Myc-AGO4. Fig. 5B ago4-npre1-1 4 10 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization we performed coimmunolocalization experiments using antibody against the native KTF1 protein and transgenic lines expressing NRPE1-Flag and Myc-AGO4. Fig. 5B rd25a-ktf1-1 2 7 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey p3 region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction RNA is 32P radio-labelled. The KTF1 P3 region binds to both the forward and reverse strands of the 40 nt RD25A RNA. RNA must be single stranded and KTF1 P3 region does not bind to the corresponding DNA region. Fig. 6