IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ GAMETOPHYTIC FACTOR 1, involved in Pre-mRNA splicing, is essential for megagametogenesis and embryogenesis in Arabidopsis. GAMETOPHYTIC FACTOR 1, involved in Pre-mRNA splicing, is essential for megagametogenesis and embryogenesis in Arabidopsis. J Integr Plant Biol (1672-9072) 2009 imex curation Liu M., Yuan L., Liu NY., Shi DQ., Liu J., Yang WC. wcyang@genetics.ac.cn 09-April-2009: Contacted by JYOTI. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Liu M., Yuan L., Liu NY., Shi DQ., Liu J., Yang WC. wcyang@genetics.ac.cn Accepted 2009-MAY-1 by JYOTI. 09-April-2009: Contacted by JYOTI. J Integr Plant Biol (1672-9072) 2009 jyoti Only protein-protein interactions imex curation clo_arath 110 kDa U5 small nuclear ribonucleoprotein component CLO CLO GFA1 MEE5 Protein CLOTHO Protein GAMETOPHYTE FACTOR 1 Protein MATERNAL EFFECT EMBRYO ARREST 5 Protein VAJRA-1 SNU114 homolog VAJ-1 F9P14.8 At1g06220 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MESSLYDEFGNYVGPEIESDRDSDDEVEDEDLQDKHLEENGSDGEQGPGGSNGWITTINDVEMENQIVLPEDKKYYPTAEEVYGEDVETLVMDEDEQPLEQPIIKPVRDIRFEVGVKDQATYVSTQFLIGLMSNPALVRNVALVGHLQHGKTVFMDMLVEQTHHMSTFNAKNEKHMKYTDTRVDEQERNISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEGVMVNTERAIRHAIQDHLPIVVVINKVDRLITELKLPPRDAYYKLRHTIEVINNHISAASTTAGDLPLIDPAAGNVCFASGTAGWSFTLQSFAKMYAKLHGVAMDVDKFASRLWGDVYYHSDTRVFKRSPPVGGGERAFVQFILEPLYKIYSQVIGEHKKSVETTLAELGVTLSNSAYKLNVRPLLRLACSSVFGSASGFTDMLVKHIPSPREAAARKVDHSYTGTKDSPIYESMVECDPSGPLMVNVTKLYPKSDTSVFDVFGRVYSGRLQTGQSVRVLGEGYSPEDEEDMTIKEVTKLWIYQARYRIPVSSAPPGSWVLIEGVDASIMKTATLCNASYDEDVYIFRALQFNTLPVVKTATEPLNPSELPKMVEGLRKISKSYPLAITKVEESGEHTILGTGELYLDSIMKDLRELYSEVEVKVADPVVSFCETVVESSSMKCFAETPNKKNKITMIAEPLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDLLAARSIWAFGPDKQGPNILLDDTLPTEVDRNLMMAVKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFDHWAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRKGMSEDVSGNKFFDEAMMVELAQQTGDLHLQMI 868DD5F6597FBB79 dexhc_arath DExH-box ATP-dependent RNA helicase DExH12 BRR2A BRR2 homolog A EMB1507 Pre-mRNA-splicing helicase BRR2A Protein EMBRYO DEFECTIVE 1507 F9H16.5 At1g20960 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MANLGGGAEAHARFKQYEYRANSSLVLTTDNRPRDTHEPTGEPETLWGKIDPRSFGDRVAKGRPQELEDKLKKSKKKERDVVDDMVNIRQSKRRRLREESVLTDTDDAVYQPKTKETRAAYEAMLGLIQKQLGGQPPSIVSGAADEILAVLKNDAFRNPEKKMEIEKLLNKIENHEFDQLVSIGKLITDFQEGGDSGGGRANDDEGLDDDLGVAVEFEENEEDDEESDPDMVEEDDDEEDDEPTRTGGMQVDAGINDEDAGDANEGTNLNVQDIDAYWLQRKISQAYEQQIDPQQCQVLAEELLKILAEGDDRVVEDKLLMHLQYEKFSLVKFLLRNRLKVVWCTRLARAEDQEERNRIEEEMRGLGPELTAIVEQLHATRATAKEREENLQKSINEEARRLKDETGGDGGRGRRDVADRDSESGWVKGQRQMLDLESLAFDQGGLLMANKKCDLPPGSYRSHGKGYDEVHVPWVSKKVDRNEKLVKITEMPDWAQPAFKGMQQLNRVQSKVYDTALFKAENILLCAPTGAGKTNVAMLTILQQLEMNRNTDGTYNHGDYKIVYVAPMKALVAEVVGNLSNRLKDYGVIVRELSGDQSLTGREIEETQIIVTTPEKWDIITRKSGDRTYTQLVRLLIIDEIHLLHDNRGPVLESIVARTLRQIETTKENIRLVGLSATLPNYEDVALFLRVDLKKGLFKFDRSYRPVPLHQQYIGISVKKPLQRFQLMNDLCYQKVLAGAGKHQVLIFVHSRKETSKTARAIRDTAMANDTLSRFLKEDSVTRDVLHSHEDIVKNSDLKDILPYGFAIHHAGLSRGDREIVETLFSQGHVQVLVSTATLAWGVNLPAHTVIIKGTQVYNPEKGAWMELSPLDVMQMLGRAGRPQYDQHGEGIIITGYSELQYYLSLMNEQLPIESQFISKLADQLNAEIVLGTVQNAREACHWLGYTYLYIRMVRNPTLYGLAPDALAKDVVLEERRADLIHSAATILDKNNLVKYDRKSGYFQVTDLGRIASYYYITHGTIATYNEHLKPTMGDIDLYRLFSLSDEFKYVTVRQDEKMELAKLLDRVPIPIKETLEEPSAKINVLLQAYISQLKLEGLSLTSDMVYITQSAGRLVRALYEIVLKRGWAQLAEKALNLSKMVGKRMWSVQTPLRQFHGLSNDILMQLEKKDLVWERYYDLSAQELGELIRSPKMGKPLHKFIHQFPKVTLSAHVQPITRTVLNVELTVTPDFLWDEKIHKYVEPFWIIVEDNDGEKILHHEYFLLKKQYIDEDHTLHFTVPIFEPLPPQYFVRVVSDKWLGSETVLPVSFRHLILPEKYPPPTELLDLQPLPVTALRNPNYEILYQDFKHFNPVQTQVFTVLYNTNDNVLVAAPTGSGKTICAEFAILRNHHEGPDATMRVVYIAPLEAIAKEQFRIWEGKFGKGLGLRVVELTGETALDLKLLEKGQIIISTPEKWDALSRRWKQRKYVQQVSLFIVDELHLIGGQHGPVLEVIVSRMRYISSQVINKIRIVALSTSLANAKDLGEWIGASSHGLFNFPPGVRPVPLEIHIQGVDISSFEARMQAMTKPTYTAIVQHAKNKKPAIVFVPTRKHVRLTAVDLMAYSHMDNPQSPDFLLGKLEELDPFVEQIREETLKETLCHGIGYLHEGLSSLDQEIVTQLFEAGRIQVCVMSSSLCWGTPLTAHLVVVMGTQYYDGRENSHSDYPVPDLLQMMGRASRPLLDNAGKCVIFCHAPRKEYYKKFLYEAFPVESQLQHFLHDNFNAEVVAGVIENKQDAVDYLTWTFMYRRLPQNPNYYNLQGVSHRHLSDHLSELVENTLSDLEASKCIEVEDEMELSPLNLGMIASYYYISYTTIERFSSLLSSKTKMKGLLEILTSASEYDMIPIRPGEEDTVRRLINHQRFSFENPKCTDPHVKANALLQAHFSRQNIGGNLAMDQRDVLLSATRLLQAMVDVISSNGWLNLALLAMEVSQMVTQGMWERDSMLLQLPHFTKDLAKRCQENPGKNIETVFDLVEMEDEERQELLKMSDAQLLDIARFCNRFPNIDLTYEIVGSEEVNPGKEVTLQVMLERDMEGRTEVGPVDSLRYPKTKEEGWWLVVGDTKTNQLLAIKRVSLQRKVKVKLDFTAPSEPGEKSYTLYFMCDSYLGCDQEYSFSVDVKGSGAGDRMEE 6D40D04E080574B6 prp8a_arath Pre-mRNA-processing-splicing factor 8A PRP8A EMB14 EMB177 EMB33 PRP8 homolog A Protein ABNORMAL SUSPENSOR 2 Protein EMBRYO DEFECTIVE 14 Protein EMBRYO DEFECTIVE 177 Protein EMBRYO DEFECTIVE 33 Protein SUPPRESSOR OF OVEREXPRESSED FCA 81 SOF81 SUS2 F18B13.15 At1g80070 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MWNNNDGMPLAPPGTGGSMMPPPPAAHPSYTALPPPSNPTPPVEPTPEEAEAKLEEKARKWMQLNSKRYGDKRKFGFVETQKEDMPPEHVRKIIRDHGDMSSKKFRHDKRVYLGALKFVPHAVFKLLENMPMPWEQVRDVKVLYHITGAITFVNEIPWVVEPIYMAQWGTMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNLLDVDPLEPIQLELDEEEDSAVHTWFYDHKPLVKTKLINGPSYRRWNLSLPIMATLHRLAGQLLSDLIDRNYFYLFDMPSFFTAKALNMCIPGGPKFEPLYRDMEKGDEDWNEFNDINKLIIRSPLRTEYRIAFPHLYNNRPRKVKLCVYHSPMIMYIKTEDPDLPAFYYDPLIHPISNTNKEKRERKVYDDEDDFALPEGVEPLLRDTQLYTDTTAAGISLLFAPRPFNMRSGRTRRAEDIPLVSEWFKEHCPPAYPVKVRVSYQKLLKCYVLNELHHRPPKAQKKKHLFRSLAATKFFQSTELDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREILRLTKLVVDANVQFRLGNVDAFQLADGLQYIFSHVGQLTGMYRYKYRLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAPMWRVWLFFLRGIVPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDVLDAMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPVPIENMILRYVKSKADWWTNVAHYNRERIRRGATVDKTVCRKNLGRLTRLWLKAEQERQHNYLKDGPYVTPEEALAIYTTTVHWLESRKFSPIPFPPLSYKHDTKLLILALERLKESYSVAVRLNQQQREELGLIEQAYDNPHEALSRIKRHLLTQRGFKEVGIEFMDLYSYLIPVYEIEPLEKITDAYLDQYLWYEGDKRHLFPNWIKPADSEPPPLLVYKWCQGINNLQGIWDTGDGQCVVMLQTKFEKFFEKIDLTMLNRLLRLVLDHNIADYVSAKNNVVLSYKDMSHTNSYGLIRGLQFASFVVQFYGLLLDLLLLGLTRASEIAGPPQMPNEFMTFWDTKVETRHPIRLYSRYIDKVHIMFKFTHEEARDLIQRYLTEHPDPNNENMVGYNNKKCWPRDARMRLMKHDVNLGRSVFWDMKNRLPRSITTLEWENGFVSVYSKDNPNLLFSMCGFEVRILPKIRMTQEAFSNTKDGVWNLQNEQTKERTAVAFLRVDDEHMKVFENRVRQILMSSGSTTFTKIVNKWNTALIGLMTYFREATVHTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVIFYTPKEIGGLGMLSMGHILIPQSDLRYSKQTDVGVTHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAQAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDVIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKYKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESVVMDLCQVLDQELDALEIETVQKETIHPRKSYKMNSSCADVLLFAAHKWPMSKPSLVAESKDMFDQKASNKYWIDVQLRWGDYDSHDIERYTRAKFMDYTTDNMSIYPSPTGVMIGLDLAYNLHSAFGNWFPGSKPLLAQAMNKIMKSNPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGEIFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKVIHTSVWAGQKRLGQLAKWKTAEEVAALVRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKIEKFGDLILKATEPQMVLFNIYDDWLKSISSYTAFSRLILILRALHVNNEKAKMLLKPDKSVVTEPHHIWPSLTDDQWMKVEVALRDLILSDYAKKNNVNTSALTQSEIRDIILGAEITPPSQQRQQIAEIEKQAKEASQLTAVTTRTTNVHGDELIVTTTSPYEQSAFGSKTDWRVRAISATNLYLRVNHIYVNSDDIKETGYTYIMPKNILKKFICVADLRTQIAGYLYGISPPDNPQVKEIRCVVMVPQWGNHQLVHLPSSLPEHDFLNDLEPLGWLHTQPNELPQLSPQDVTSHSRILENNKQWDGEKCIILTCSFTPGSCSLTSYKLTQTGYEWGRLNKDNGSNPHGYLPTHYEKVQMLLSDRFLGFYMVPESGPWNYSFTGVKHTLSMKYSVKLGSPKEFYHEEHRPTHFLEFSNMEEADITEGDREDTFT A2E2A74BCB024DE3 gfa1-brr2a-1 1 3 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 2 unspecified role unspecified role bait bait physical association physical association Figure 7C Protein-protein interactions of GFA1 and AtBrr2a, AtPrp8a. gfa1-brr2a-2 1 2 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 7C Protein-protein interactions of GFA1 and AtBrr2a, AtPrp8a. The UniProt record for protein Q9SSD2 (At1g80070) has an amino acid length of 2359 whereas the TAIR protein for At1g80070 is 2382 amino acids in length. The difference occurs because of a 23 amino acid sequence missing in the UniProt record starting at amino acid 95. Note the authors state the At1g80070 protein is 2383 amino acids in length. The authors used the last 308 amino acids of the protein in the prey construct which is equivalent to amino acids 2051 - 2359 of the UniProt record. This is the region annotated in this entry.